WO2005059547A3 - Assay - Google Patents

Assay Download PDF

Info

Publication number
WO2005059547A3
WO2005059547A3 PCT/GB2004/005146 GB2004005146W WO2005059547A3 WO 2005059547 A3 WO2005059547 A3 WO 2005059547A3 GB 2004005146 W GB2004005146 W GB 2004005146W WO 2005059547 A3 WO2005059547 A3 WO 2005059547A3
Authority
WO
WIPO (PCT)
Prior art keywords
species
immobilized
binding
analyte
capture reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2004/005146
Other languages
French (fr)
Other versions
WO2005059547A2 (en
Inventor
Balbir Raj
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alere Switzerland GmbH
Original Assignee
Inverness Medical Switzerland GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0328892A external-priority patent/GB0328892D0/en
Priority to HK07102438.9A priority Critical patent/HK1099360B/en
Application filed by Inverness Medical Switzerland GmbH filed Critical Inverness Medical Switzerland GmbH
Priority to CN2004800370304A priority patent/CN1894585B/en
Priority to AT04805966T priority patent/ATE449337T1/en
Priority to AU2004298660A priority patent/AU2004298660B2/en
Priority to JP2006543616A priority patent/JP4624364B2/en
Priority to DE602004024236T priority patent/DE602004024236D1/en
Priority to CA2548164A priority patent/CA2548164C/en
Priority to EP04805966A priority patent/EP1706743B1/en
Publication of WO2005059547A2 publication Critical patent/WO2005059547A2/en
Publication of WO2005059547A3 publication Critical patent/WO2005059547A3/en
Priority to US11/423,581 priority patent/US7901949B2/en
Anticipated expiration legal-status Critical
Priority to US13/018,828 priority patent/US20110223690A1/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The present invention provides an assay kit for detecting an analyte of interest in a sample. The kit comprises a) a reporter species; b) a labeled species having first and second binding regions, wherein the first binding region is capable of binding to the analyte of interest and the second binding region is capable of binding to the reporter species; c) an immobilized species capable of binding to the first binding region of the labeled species; and d) immobilized capture reagent capable of binding to the reporter species. The arrangement is such that the sample is contacted with the labeled species, is then contacted with the immobilized species and is subsequently contacted with the immobilized capture reagent, the reporter species being added prior to exposure of the sample to the immobilized capture reagent. If no analyte is present in the sample, the labeled species becomes bound to the immobilized species and is therefore unable to bind to the immobilized capture reagent. If analyte is present in the sample, the analyte binds to the labeled species such that the labeled species is unable to bind to the immobilized species but can bind to the immobilized capture reagent via the reporter species, the presence of the analyte thus being determined by the presence of labeled species bound to the immobilized capture reagent via the reporter species.
PCT/GB2004/005146 2003-12-12 2004-12-10 Assay Ceased WO2005059547A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
CA2548164A CA2548164C (en) 2003-12-12 2004-12-10 Assay kit for detecting analytes of interest in samples
DE602004024236T DE602004024236D1 (en) 2003-12-12 2004-12-10 Assay
CN2004800370304A CN1894585B (en) 2003-12-12 2004-12-10 An assay device and assay method for detecting an analyte of interest in a sample
AT04805966T ATE449337T1 (en) 2003-12-12 2004-12-10 ASSAY
AU2004298660A AU2004298660B2 (en) 2003-12-12 2004-12-10 Assay
JP2006543616A JP4624364B2 (en) 2003-12-12 2004-12-10 Assay
EP04805966A EP1706743B1 (en) 2003-12-12 2004-12-10 Assay
HK07102438.9A HK1099360B (en) 2003-12-12 2004-12-10 Assay
US11/423,581 US7901949B2 (en) 2003-12-12 2006-06-12 Assay
US13/018,828 US20110223690A1 (en) 2003-12-12 2011-02-01 Assay

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0328892.5 2003-12-12
GB0328892A GB0328892D0 (en) 2003-12-12 2003-12-12 Positive signal assay for analytes
US58511804P 2004-07-01 2004-07-01
US60/585,118 2004-07-01

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/423,581 Continuation US7901949B2 (en) 2003-12-12 2006-06-12 Assay

Publications (2)

Publication Number Publication Date
WO2005059547A2 WO2005059547A2 (en) 2005-06-30
WO2005059547A3 true WO2005059547A3 (en) 2005-11-03

Family

ID=34702506

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/005146 Ceased WO2005059547A2 (en) 2003-12-12 2004-12-10 Assay

Country Status (8)

Country Link
US (2) US7901949B2 (en)
EP (1) EP1706743B1 (en)
JP (1) JP4624364B2 (en)
AT (1) ATE449337T1 (en)
AU (1) AU2004298660B2 (en)
CA (1) CA2548164C (en)
DE (1) DE602004024236D1 (en)
WO (1) WO2005059547A2 (en)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8470608B2 (en) * 2008-05-20 2013-06-25 Rapid Pathogen Screening, Inc Combined visual/fluorescence analyte detection test
US8669052B2 (en) 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
GB0524746D0 (en) * 2005-12-03 2006-01-11 Inverness Medical Switzerland Assay device and method
CN101021526B (en) * 2007-03-16 2012-01-25 艾博生物医药(杭州)有限公司 Apparatus and method for visual display of detecting result
US8609433B2 (en) 2009-12-04 2013-12-17 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with sample compressor
US20130196310A1 (en) 2008-05-20 2013-08-01 Rapid Pathogen Screening, Inc. Method and Device for Combined Detection of Viral and Bacterial Infections
US9068981B2 (en) 2009-12-04 2015-06-30 Rapid Pathogen Screening, Inc. Lateral flow assays with time delayed components
US8962260B2 (en) 2008-05-20 2015-02-24 Rapid Pathogen Screening, Inc. Method and device for combined detection of viral and bacterial infections
US8815609B2 (en) 2008-05-20 2014-08-26 Rapid Pathogen Screening, Inc. Multiplanar lateral flow assay with diverting zone
US20110086359A1 (en) * 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
US9523701B2 (en) 2009-07-29 2016-12-20 Dynex Technologies, Inc. Sample plate systems and methods
GB0913258D0 (en) 2009-07-29 2009-09-02 Dynex Technologies Inc Reagent dispenser
US8088594B2 (en) * 2010-03-16 2012-01-03 Saladax Biomedical Inc. Risperidone immunoassay
US8486717B2 (en) 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
JP6352258B2 (en) 2012-07-16 2018-07-04 センターズ フォー ディジーズ コントロール アンド プリベンション Direct-reading detection kit for surface contamination with antitumor agents
US9874556B2 (en) 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
CA2882562C (en) 2012-08-21 2019-08-27 Eric Hryhorenko Antibodies to aripiprazole and use thereof
CN104736565B (en) * 2012-08-21 2019-03-12 詹森药业有限公司 Antibody to risperidone hapten and its use
ES2654413T3 (en) 2012-08-21 2018-02-13 Janssen Pharmaceutica, N.V. Quetiapine antibodies and their use
ES2978909T3 (en) * 2012-08-21 2024-09-23 Janssen Pharmaceutica Nv Antibodies to aripiprazole haptens and their use
ES2788716T3 (en) 2012-08-21 2020-10-22 Janssen Pharmaceutica Nv Antibodies to Quetiapine Haptens and their Use
EP3462173B1 (en) 2012-08-21 2021-03-31 Janssen Pharmaceutica NV Antibodies to risperidone and use thereof
CA2882490A1 (en) 2012-08-21 2014-02-27 Ortho-Clinical Diagnostics, Inc. Antibodies to paliperidone and use thereof
ES2652641T3 (en) 2012-08-21 2018-02-05 Janssen Pharmaceutica Nv Haptenosis of aripiprazole and its use in immunoassays
JP6389177B2 (en) 2012-08-21 2018-09-12 ヤンセン ファーマシューティカ エヌ．ベー． Antibodies against olanzapine hapten and use thereof
JP2015529199A (en) 2012-08-21 2015-10-05 オルソ−クリニカル ダイアグノスティクス，インコーポレイティド Antibodies against paliperidone hapten and use thereof
US9494608B2 (en) 2012-08-21 2016-11-15 Janssen Pharmaceutica Nv Antibodies to olanzapine and use thereof
US10890590B2 (en) 2012-09-27 2021-01-12 Ellume Limited Diagnostic devices and methods
EP3044592B1 (en) 2013-09-13 2019-07-17 Symbolics, LLC Lateral flow assays using two dimensional test and control signal readout patterns
WO2016115608A1 (en) 2015-01-22 2016-07-28 Ellume Pty Ltd Diagnostic devices and methods for mitigating hook effect and use thereof
ES2903034T3 (en) * 2015-07-15 2022-03-30 Gestvision Inc Device for the detection of misfolded proteins and methods for its use
US10808287B2 (en) 2015-10-23 2020-10-20 Rapid Pathogen Screening, Inc. Methods and devices for accurate diagnosis of infections
CN108431040B (en) 2015-12-17 2022-07-26 詹森药业有限公司 Antibodies to risperidone and uses thereof
EP3390455A1 (en) 2015-12-17 2018-10-24 Janssen Pharmaceutica NV Antibodies to quetiapine and use thereof
US10871485B2 (en) 2018-04-13 2020-12-22 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
GB201905090D0 (en) 2019-04-10 2019-05-22 Spd Swiss Prec Diagnostics Gmbh Assay device
CN112730825B (en) * 2020-12-17 2022-09-06 北京丹大生物技术有限公司 Detection reagent and detection kit for detecting risperidone and/or 9-hydroxy risperidone

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995027081A1 (en) * 1994-03-31 1995-10-12 E.I. Du Pont De Nemours And Company A method for detection of nucleic acid fragments
US5874216A (en) * 1996-02-23 1999-02-23 Ensys Environmental Products, Inc. Indirect label assay device for detecting small molecules and method of use thereof
US20020142291A1 (en) * 2000-04-14 2002-10-03 Bauer Jeffrey S. Positive detection lateral-flow apparatus and method for small and large analytes

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE195022T1 (en) 1987-04-27 2000-08-15 Unilever Nv SPECIFIC BINDING TESTING METHODS
US5939272A (en) 1989-01-10 1999-08-17 Biosite Diagnostics Incorporated Non-competitive threshold ligand-receptor assays
US6352862B1 (en) 1989-02-17 2002-03-05 Unilever Patent Holdings B.V. Analytical test device for imuno assays and methods of using same
GB8903627D0 (en) 1989-02-17 1989-04-05 Unilever Plc Assays
US5296347A (en) * 1991-02-08 1994-03-22 Ciba Corning Diagnostics Corp. Bridge immunoassay
AU1772992A (en) 1991-04-10 1992-11-17 Biosite Diagnostics Incorporated Crosstalk inhibitors and their uses
US5451504A (en) 1991-07-29 1995-09-19 Serex, Inc. Method and device for detecting the presence of analyte in a sample
US6037127A (en) 1994-03-31 2000-03-14 E. I. Du Pont De Nemours And Company Method for detection of non-denatured nucleic acid fragments
ATE228247T1 (en) 1996-09-27 2002-12-15 Inverness Medical Switzerland TEST REAGENTS AND TEST DEVICES
SE9704933D0 (en) * 1997-12-30 1997-12-30 Pharmacia & Upjohn Diag Ab Method utilizing a new calibrator and test kit containing the calibrator
US6753189B1 (en) 1998-06-04 2004-06-22 Mizuho Medy Co., Ltd. Detection apparatus and method for the same
US6414133B1 (en) * 1998-10-13 2002-07-02 Ventana Medical Systems, Inc. Multiple fusion probes
DE19927783A1 (en) * 1999-06-18 2000-12-21 Roche Diagnostics Gmbh Element for determining an analyte in a liquid, corresponding determination method using the element and kit for determining an analyte
DE10009503A1 (en) * 2000-02-29 2001-08-30 Roche Diagnostics Gmbh Procedure for immobilizing conjugates in diagnostic tests
US7029918B2 (en) * 2002-01-25 2006-04-18 Roche Diagnostics Operations, Inc. Water-soluble derivatives of lipophilic drugs
US8101429B2 (en) * 2003-06-03 2012-01-24 Siemens Healthcare Diagnostics Inc. Native analyte as a reference in lateral flow assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995027081A1 (en) * 1994-03-31 1995-10-12 E.I. Du Pont De Nemours And Company A method for detection of nucleic acid fragments
US5874216A (en) * 1996-02-23 1999-02-23 Ensys Environmental Products, Inc. Indirect label assay device for detecting small molecules and method of use thereof
US20020142291A1 (en) * 2000-04-14 2002-10-03 Bauer Jeffrey S. Positive detection lateral-flow apparatus and method for small and large analytes

Also Published As

Publication number Publication date
EP1706743A2 (en) 2006-10-04
US7901949B2 (en) 2011-03-08
US20070015290A1 (en) 2007-01-18
EP1706743B1 (en) 2009-11-18
WO2005059547A2 (en) 2005-06-30
JP4624364B2 (en) 2011-02-02
CA2548164A1 (en) 2005-06-30
AU2004298660A1 (en) 2005-06-30
HK1099360A1 (en) 2007-08-10
US20110223690A1 (en) 2011-09-15
CA2548164C (en) 2013-10-08
JP2007514157A (en) 2007-05-31
DE602004024236D1 (en) 2009-12-31
ATE449337T1 (en) 2009-12-15
AU2004298660B2 (en) 2010-04-22

Similar Documents

Publication Publication Date Title
WO2005059547A3 (en) Assay
WO2008051762A3 (en) Immunoassay of analytes in samples containing endogenous anti-analyte antibodies
EP2063269A3 (en) Chromatography apparatus for detecting Staphylococcus aureus
AU2002304640A1 (en) Method and diagnosis kit for selecting and or qualitative and/or quantitative detection of cells
WO2004041061A8 (en) Substrates, devices, and methods for cellular assays
NO20025371L (en) Improvements to fluorescence polymer-QTL approach for bio-detection
WO2005047863A3 (en) Substrates, devices, and methods for cellular assays
DE60136034D1 (en) Molecular modification assays
WO2007106598A3 (en) Devices and methods for detecting cells and other analytes
WO1999022242A3 (en) Measurement of glycated hemoglobin
WO2012038820A3 (en) Immunochromatography devices, methods and kits
WO2003023051A3 (en) Lateral flow test format for enzyme assays
WO2006047591A3 (en) Rapid microfluidic assay for analyte interactions
DE602006010242D1 (en) IMMUNOASSAY WITH DOUBLE STANDARD CURVE
ATE405837T1 (en) SOLID PHASE IMMUNOCHROMATOGRAPHIC TESTING METHODS
EP1138781A3 (en) Method for Quantifying DNA binding activity of DNA binding proteins
WO2007024633A3 (en) Multi-directional immunochromatographic assays
SE0301058D0 (en) Method and kit for cell analyte assay
WO2008021071A3 (en) Substrates, devices, and methods for cellular assays
WO2006103450A3 (en) Electrochemical assay
WO2007076013A3 (en) Homocysteine immunoassay
WO2004039963A3 (en) Antibodies specific for phosphorylated insulin receptor substrate-1/2 (ser 1101/ser1149) and uses thereof
EP2674757A3 (en) Agglutination assay
DK1057021T3 (en) Method for measuring total analyte
WO2005079420A3 (en) Chromatographic exclusion agglutination assay and methods of use thereof

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 200480037030.4

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2004298660

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2548164

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2006543616

Country of ref document: JP

Ref document number: 3347/DELNP/2006

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 11423581

Country of ref document: US

ENP Entry into the national phase

Ref document number: 2004298660

Country of ref document: AU

Date of ref document: 20041210

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2004298660

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2004805966

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004805966

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 11423581

Country of ref document: US