WO2005075501A1 - Polyoxyalkylene compound and method for making - Google Patents

Polyoxyalkylene compound and method for making Download PDF

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WO2005075501A1
WO2005075501A1 PCT/US2005/003201 US2005003201W WO2005075501A1 WO 2005075501 A1 WO2005075501 A1 WO 2005075501A1 US 2005003201 W US2005003201 W US 2005003201W WO 2005075501 A1 WO2005075501 A1 WO 2005075501A1
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compound
nmr
polyethylene glycol
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derived
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Dillip K. Mohanty
Ajit Sharma
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Central Michigan University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C251/00—Compounds containing nitrogen atoms doubly-bound to a carbon skeleton
    • C07C251/02—Compounds containing nitrogen atoms doubly-bound to a carbon skeleton containing imino groups
    • C07C251/24—Compounds containing nitrogen atoms doubly-bound to a carbon skeleton containing imino groups having carbon atoms of imino groups bound to carbon atoms of six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C229/06—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton
    • C07C229/10—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings
    • C07C229/14—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings to carbon atoms of carbon skeletons containing rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C229/06—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton
    • C07C229/10—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings
    • C07C229/16—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to acyclic carbon atoms or to carbon atoms of rings other than six-membered aromatic rings to carbon atoms of hydrocarbon radicals substituted by amino or carboxyl groups, e.g. ethylenediamine-tetra-acetic acid, iminodiacetic acids

Definitions

  • the instant invention is in the field of chemical compounds comprising polyoxyalkylene sub-structures such as polyethylene glycol sub-structures.
  • the instant invention also relates to methods for producing chemical compounds comprising polyoxyalkylene sub-structures.
  • Biologically active compounds comprising polyoxyalkylene sub-structures can provide enhanced biocompatibility for the compound, See, for example, USP 5,366,735 and USP 6,280,745.
  • polyethylene glycol as one of the best biocompatible polymers to conjugate with a biologically active compound (such as a drug, a protein, a peptide or an enzyme) to produce a conjugate having improved properties such as compatible solubility characteristics, reduced toxicity and reduced immunogenicity.
  • a biologically active compound such as a drug, a protein, a peptide or an enzyme
  • PEG polyethylene glycol
  • monomethoxy polyethylene glycol As discussed by Henmanson in Chapter 15 of Bioconjugate Techniques (1966), monomethoxy polyethylene glycol (mPEG) generally represented by the formula: CH 3 0- (CH 2 CH 2 0) n - CH 2 CH 2 -OH, is usually used to prepare a polyethylene glycol conjugate with a biologically active compound typically by way of a coupling reaction between an amine group of the biologically active compound and an amine receptive derivative (such as trichloro-s-triazine activated mPEG) formed via the remaining terminal hydroxyl group of the monomethoxy polyethylene glycol.
  • amine receptive derivative such as trichloro-s-triazine activated mPEG
  • An acrylate terminated PEG is offered commercially by Shearwater Corporation (Huntsville, AL) for vinyl polymerization or co-polymerization to produce graft polymers or cross-linked materials with excellent properties for biomaterial applications. More recently, so called "second generation" PEGylation chemistry has been developed to, for example, minimize problems of diol impurity contamination of mPEG, to increase the molecular weight of the polyoxyalkylene sub-structure and to increase stability of the conjugate, see Roberts et al., Advanced Drug Delivery Reviews 54 (2002) p459-476. Dendrimers are hyperbranched, uniformLy distributed structures, having (at least ideally) definite molecular weight, shape and nanometer size characteristics.
  • Dendrimers were discovered by Tomalia and co-workers at the Dow Chemical Company, see Poly . J. 17 (1985) pll7-132. Dendrimers have been widely studied as a drug delivery means, see for example Knusli et al., J. Haematology, 82, 654 (1992). Dendrimers carrying the anti cancer drug 5-fluorouracil have been PEGylated to reduce hemolytic toxicity, drug leakage and macrophageal uptake while improving stability and efficacy, see Bhadra et al., International Journal of Pharmaceutics 257 (2003) plll-124.
  • PAMAM dendrimers are the most common type of dendrimer and are commercially available from Aldrich (Milwaukee, WI) in the form of various "generations". PAMAM dendrimers are made by a successive Michael addition synthesis scheme involving the reaction of an acrylate group with an amine group. The so called “Generation 0" PAMAM dendrimer has the following formula:
  • the above Generation 0 PAMAM dendrimer has a molecular weight of about 517 grams per mole.
  • a Generation 1 PAMAM dendrimer has a molecular weight of about 1,430 grams per mole and has eight terminal primary amine groups.
  • a Generation 2 PAMAM dendrimer has a molecular weight of about 3,256 grams per mole and has sixteen terminal primary amine groups.
  • a Generation 10 PAMAM dendrimer has a theoretical molecular weight of almost 935 kilograms per mole and in theory has 4096 primary amine groups on the surface of the dendrimer.
  • the method of the instant invention is a solution, at least in part, to the above described problems of the prior art.
  • the instant invention provides a one step pegylation method that ideally produces no by-products.
  • the method of the instant invention can be practiced at room temperature and under conditions such as solvent compatibility that are mild relative to maintenance of biological activity.
  • the instant invention is applicable to biologically active compounds containing an amine group.
  • the instant invention is applicable to biologically active compounds containing a sulfide group.
  • the invention is the compound of formula 1 and the compound of formula 2 as described above.
  • IR spectra are obtained using a thin film on a sodium chloride plate. Spectra are recorded using a Nicolet 20DXB Fourier Transform (FT-IR) Spectrometer and absorption is reported in wave number (cm -1 ) . IR spectra cover the range 1000-4000 cm -1 .
  • Proton nuclear magnetic resonance spectra and carbon-13 nuclear magnetic resonance spectra are recorded for solutions in appropriate solvents containing tetramethylsilane in case of chloroform and methanol and 3 (trimethylsilyl) propane Sulfonic acid sodium salt (DSS) in case of Deuterium Oxide as internal standard using a General Electric QE-300 NMR spectrometer.
  • the NMR shifts are reported in parts per million ( ⁇ , PPM) .
  • Mass Spectra are obtained by using a Hewlett-Packard Model 5995A gas Chromatograph/Mass Spectrometer with an ionizing potential of 70 electron volts.
  • PAMAM Dendrimer is purchased in 20% solution in methanol and used without further purification.
  • a PEG chain may be represented:
  • N-EthylBenzyl amine-PEGMEA conjugates Structure: Reactive
  • Benzaldehyde and acetophenone were used for above purpose (and almost any aldehyde or ketone can be used for this purpose and an aldehyde substituted cyclodextrin is believed to be especially useful in this respect) .
  • Model reactions 6.a and 6.b show that formation of Schiff base followed by reduction of imine functional group can be achieved.
  • the presence of absorbance at 1642 cm “1 confirm the formation of imine.
  • the absence of that absorbance in IR spectrum after reduction proved that imine functional group can be reduced.
  • the synthesis routes are shown in scheme 7 and 8 to remove the hydrogen atoms attached to residual primary amine groups.
  • Aldehyde substituted beta cyclodextrins are especially useful in the instant invention to block primary amines.
  • an amine terminated polyethylene glycol can be reacted with an aldehyde substituted beta cyclodextrin at room temperature in aqueous sodium cyanohydridoborate to couple the cyclodextrine to the polyethylene glycol via a nitrogen atom to form a pegylated cyclodextrine adduct.
  • the pegylated cyclodextrine adduct can be reacted with the polyoxyalkylene acrylate to form a cyclodextrin adduct that is further pegylated.
  • Chitosan can be reacted with an aldehyde substituted beta cyclodextrin at room temperature in aqueous sodium cyanohydridoborate to block the primary amines of the chitosan followed by reaction with the polyoxyalkylene acrylate to form a pegylated and beta cyclodextrin substituted chitosan.
  • Peptides, polypeptides and proteins containing primary amines can be reacted with an aldehyde substituted beta cyclodextrin at room temperature in aqueous sodium cyanohydridoborate to block the primary amines of the peptide, polypeptide or protein followed by reaction with the polyoxyalkylene acrylate to form a pegylated and beta cyclodextrin substituted peptide, polypeptide or protein.
  • the following scheme can be used in the instant invention to first convert the primary amines of poly-L-arginine to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate.
  • a 3 necked, 25-mL, round- bottomed, flask is fitted with nitrogen inlet, a condenser with drying tube, a rubber septum, and a magnetic stir bar.
  • the following ingredients are added to the flask: poly-L-arginine hydrochloride (5mg, 0.663 ⁇ mol), beta cyclodextrin monoaldehyde
  • reaction mixture is then stirred for an additional 72 hours at room temperature and then precipitated in 10-mL of acetone and centrifuged to yield a white sold that was dried under vacuum overnight.
  • Analysis of the white solid confirms the desired formation of a pegylated beta cyclodextrin-poly-L-lysine conjugate.
  • the following scheme can be used in the instant invention to first convert the primary amines of Chitosan to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate.
  • a 3 necked, 25-mL, round- bottomed, flask is fitted with a nitrogen inlet, a condenser with drying tube, a rubber septum and a magnetic stir bar.
  • the following ingredients are added to the flask: low molecular weight chitosan (50mg, 0.185mmol) dissolved in 15-mL of 0.1M hydrochloric acid, 2.00g of beta-glucero-phosphate dissolved in 4-mL of deionized water, beta cyclodextrin monoaldehyde (421mg, 0.370mmol) and sodium cyanoborohydride (33 mg, 0.525mmol).
  • low molecular weight chitosan 50mg, 0.185mmol
  • beta-glucero-phosphate dissolved in 4-mL of deionized water
  • beta cyclodextrin monoaldehyde 421mg, 0.370mmol
  • sodium cyanoborohydride 33 mg, 0.525mmol
  • the clear aqueous solution is allowed to mix on a tabletop shaker for 3h at room temperature.
  • the entire reaction mixture is lypholized to produce the desired pegylated glutathione.
  • the following scheme can be used in the instant invention for the pegylation of proteins: (a) the protein is dissolved or dispersed in 0.1M bicarbonate buffer, pH 9.1 (the concentration of proteins as a rule can be measured from their extinction coefficients at 280 nm) ; (b) mPEG acrylate solutions are prepared at various concentrations in 0.1M bicarbonate buffer, pH 9.1; (c) a known volume of the protein solution is mixed with the mPEG solution in various vials to yield various amino / mPEG ratios; (d) samples are incubated under defined temperatures and times with appropriate control tubes; and (e) after reaction, the reaction mixture is subjected to native gel electrophoresis in 10% polyacrylamide gels (protein staining, as a rule, is performed with Coomassie Blue).
  • the following scheme can be used in the instant invention to first convert the primary amines of a PAMAM dendrimer to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate.
  • a 3 necked, 25-mL, round- bottomed, flask is fitted with a nitrogen inlet, a condenser with drying tube, a rubber septum and a magnetic stir bar.
  • PAMAM generation 0 dendrimer 160mg, 0.310mmol dissolved in 1- mL deionized water
  • beta cyclodextrin monoaldehyde (1.5010g, 1.32mmol) dissolved in 15-mL of deionized water
  • sodium cyanoborohydride (229.6mg, 3.65mmol).
  • the reaction mixture is then stirred for 72 hours at room temperature.
  • 6-mL of the reaction mixture is then removed and precipitated in methanol to confirm the production of the desired product.
  • 9-mL poly (ethylene glycol) methyl ether acrylate aqueous solution (0.0880M) are added.
  • the amine group is a primary amine
  • Many drug compounds contain amine group (s) and it should be understood that the instant invention is an excellent means of converting such drugs to a polyoxyalkylene conjugate of the drug.
  • polyoxyalkylene is defined in the above referenced US Patent 6,280,745, herein fully incorporated by reference, and includes polyethylene glycol, polypropylene glycol, as well as block and random polyethylene glycol/polypropylene glycol co-polymers.
  • acrylate terminated polyethylene glycols are commercially available, acrylate terminated polyethylene glycol can be prepared, for example, by reacting a monomethoxy polyethylene glycol with acryloyl chloride or, for example, with methacroloyl chloride.
  • the molecular weight of the polyoxyalkylene sub-structure of the instant invention can be tailored so that the conjugate has desired properties such as solubility characteristics that are more compatible with the biologic system.
  • the preferred molecular weight of the polyoxyalkylene sub- structure of the instant invention will be in the range of from about 500 to about 5000 grams per mole.
  • the acrylate terminated polyoxylakylene of the instant invention also can be reacted with a terminal sulfur (sulfide) group (s) of a biologically active compound to produce novel compounds.
  • Polycysteine can be similarly pegylated. The process of the instant invention produces novel compounds that, as expected, maintain their biological activity.
  • bovine erythrocyte carbonic anhydrase (CAB) pegylated with mPEG acrylate at room temperature in a pH 9.1 aqueous buffer (mole ratio of CAB to mPEG acrylate of 1:8; 1:2 and 8:1) maintains its biological activity.
  • hen egg white lysozyme (HEWL) pegylated with mPEG acrylate at room temperature in a pH 9.1 aqueous buffer (mole ratio of HEWL to mPEG acrylate of 1:2 and 8:1) also maintains its biological activity.

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Abstract

A process for forming a conjugate of a polyoxyalkylene polymer (such as polyethylene glycol) with a compound containing an amine group(s) and/or a sulfide group(s) by reacting the compound with an acrylate terminated polyoxyalkylene (such as polyethylene glycol terminate at one end with acrylate or methacrylate and terminated at the other end with a methoxy group). The reaction is believed to be a Michael addition. When the compound contains primary amine groups (such as the surface primary amine groups of a PAMAM dendrimer), it is usually desirable to convert the primary amine groups to secondary amine groups before the reaction with the acrylate terminated polyoxyalkylene.

Description

POLYOXYALKYLENE COMPOUND AND METHOD FOR MAKING This application claims priority from US Application No. 10/770,224 filed February 3, 2004. BACKGROUND The instant invention is in the field of chemical compounds comprising polyoxyalkylene sub-structures such as polyethylene glycol sub-structures. The instant invention also relates to methods for producing chemical compounds comprising polyoxyalkylene sub-structures. Biologically active compounds comprising polyoxyalkylene sub-structures can provide enhanced biocompatibility for the compound, See, for example, USP 5,366,735 and USP 6,280,745. A review of this subject by Zalipsky, in Bioconjugate Chem., 1995, 6, pl50-165, identified polyethylene glycol as one of the best biocompatible polymers to conjugate with a biologically active compound (such as a drug, a protein, a peptide or an enzyme) to produce a conjugate having improved properties such as compatible solubility characteristics, reduced toxicity and reduced immunogenicity. Polyethylene glycol (PEG) is a linear or branched polyoxyalkylene terminated at the ends thereof with hydroxyl groups and generally represented by the formula: HO- (CH2CH20) n- CH2CH2-OH. As discussed by Henmanson in Chapter 15 of Bioconjugate Techniques (1966), monomethoxy polyethylene glycol (mPEG) generally represented by the formula: CH30- (CH2CH20) n- CH2CH2-OH, is usually used to prepare a polyethylene glycol conjugate with a biologically active compound typically by way of a coupling reaction between an amine group of the biologically active compound and an amine receptive derivative (such as trichloro-s-triazine activated mPEG) formed via the remaining terminal hydroxyl group of the monomethoxy polyethylene glycol. An acrylate terminated PEG is offered commercially by Shearwater Corporation (Huntsville, AL) for vinyl polymerization or co-polymerization to produce graft polymers or cross-linked materials with excellent properties for biomaterial applications. More recently, so called "second generation" PEGylation chemistry has been developed to, for example, minimize problems of diol impurity contamination of mPEG, to increase the molecular weight of the polyoxyalkylene sub-structure and to increase stability of the conjugate, see Roberts et al., Advanced Drug Delivery Reviews 54 (2002) p459-476. Dendrimers are hyperbranched, uniformLy distributed structures, having (at least ideally) definite molecular weight, shape and nanometer size characteristics. Dendrimers were discovered by Tomalia and co-workers at the Dow Chemical Company, see Poly . J. 17 (1985) pll7-132. Dendrimers have been widely studied as a drug delivery means, see for example Knusli et al., J. Haematology, 82, 654 (1992). Dendrimers carrying the anti cancer drug 5-fluorouracil have been PEGylated to reduce hemolytic toxicity, drug leakage and macrophageal uptake while improving stability and efficacy, see Bhadra et al., International Journal of Pharmaceutics 257 (2003) plll-124. PAMAM dendrimers are the most common type of dendrimer and are commercially available from Aldrich (Milwaukee, WI) in the form of various "generations". PAMAM dendrimers are made by a successive Michael addition synthesis scheme involving the reaction of an acrylate group with an amine group. The so called "Generation 0" PAMAM dendrimer has the following formula:
Figure imgf000003_0001
The above Generation 0 PAMAM dendrimer has a molecular weight of about 517 grams per mole. A Generation 1 PAMAM dendrimer has a molecular weight of about 1,430 grams per mole and has eight terminal primary amine groups. A Generation 2 PAMAM dendrimer has a molecular weight of about 3,256 grams per mole and has sixteen terminal primary amine groups. A Generation 10 PAMAM dendrimer has a theoretical molecular weight of almost 935 kilograms per mole and in theory has 4096 primary amine groups on the surface of the dendrimer. Despite the significant advances that have been made in the field of methods for the PEGylation of biologically active compounds (and more generally in the field of methods for the conjugation of polyoxyalkylene sub-structures with biologically active compounds) , the existing methods generally require multiple reactions and extensive purification of the product.
It would be an advance in this art if a process were discovered that required only one reaction step and produced no byproducts.
SUMMARY OF THE INVENTION The method of the instant invention is a solution, at least in part, to the above described problems of the prior art. The instant invention provides a one step pegylation method that ideally produces no by-products. In addition, the method of the instant invention can be practiced at room temperature and under conditions such as solvent compatibility that are mild relative to maintenance of biological activity. In one embodiment, the instant invention is applicable to biologically active compounds containing an amine group. In another embodiment, the instant invention is applicable to biologically active compounds containing a sulfide group. The biologically active compound is reacted with an acrylate terminated polyoxyalkylene (such as H2C=CH-CO-0-PEG-0-CH3) in a one step process to produce novel conjugates having many if not all of the benefits of the prior art conjugates. More specifically, the instant invention is a method for preparing a compound corresponding to the formula: formula 1 R2 R30 I I II Rι-N-CH2-C-C-0-R4-0-R5 where Ri is an organic radical where R2 is H or an organic radical where R3 is H or an organic radical where R4 is a polyoxyalkylene radical and where R5 is an organic radical or H, comprising the step of: reacting A with B, wherein A is Rι~N-R2 and wherein R30 B is C=C-C-0-R-0-R5.
In another embodiment, the instant invention is a method for preparing a compound corresponding to the formula: formula 2 R70 i // R6-S-CH2-C-C-0-R8-0-R9 where R6 is an organic radical where R is H or an organic radical where R8 is a polyoxyalkylene radical and where R9 is an organic radical or H, comprising the step of: reacting D with E, wherein D is R6-S and wherein R70 l II E is C=C-C-0-R8-0-R9. In addition, the invention is the compound of formula 1 and the compound of formula 2 as described above.
DETAILED DESCRIPTION OF THE INVENTION In general, the process of the instant invention can be conducted at room temperature. Infrared (IR) spectra are obtained using a thin film on a sodium chloride plate. Spectra are recorded using a Nicolet 20DXB Fourier Transform (FT-IR) Spectrometer and absorption is reported in wave number (cm-1) . IR spectra cover the range 1000-4000 cm-1. Proton nuclear magnetic resonance spectra and carbon-13 nuclear magnetic resonance spectra are recorded for solutions in appropriate solvents containing tetramethylsilane in case of chloroform and methanol and 3 (trimethylsilyl) propane Sulfonic acid sodium salt (DSS) in case of Deuterium Oxide as internal standard using a General Electric QE-300 NMR spectrometer. The NMR shifts are reported in parts per million (δ, PPM) . The following standard abbreviations were used in describing NMR data: s = singlet, d = doublet, t = triplet, q = quartet, = multiplet. Mass Spectra are obtained by using a Hewlett-Packard Model 5995A gas Chromatograph/Mass Spectrometer with an ionizing potential of 70 electron volts. Starting Materials
1. PAMAM Dendrimer (Generation = 0 ) Source : Aldrich Chemical ; Structure :
Figure imgf000007_0001
The structure above may also be represented
H2N
Figure imgf000007_0002
H2N
Note: PAMAM Dendrimer is purchased in 20% solution in methanol and used without further purification.
2. Poly Ethylene glycol methyl ether acrylate Source: Aldrich Chemical Company
Structure:
double bond
Figure imgf000008_0001
-0 C ?— CH: O CHj— Ciτ-OCH3 PEGM n
Used without further purification. A PEG chain may be represented:
Figure imgf000008_0002
3. Ethylene diamine Source: Aldrich Chemical Company Structure: H H H2N—C-C-NH2 π π
Purification procedure: Ethylene diamine is distilled over
CaH2.
4. Benzaldehyde Source: Fisher Scientific Structure:
Figure imgf000008_0003
Purification procedure: Benzaldehyde (about 75 mL) is placed in a separatory funnel. It is washed with 10% NaC03 until no more C02 evolved. Saturated NaCl solution is added to the solution. The solution is then washed with a saturated solution of Na2S03 followed by washing with water. The organic layer is collected and dried with MgS0. The organic layer is filtered and then distilled under vacuum. 5. Acetophenone Source: Fisher Scientific Structure: .
used without further purification. 6. Propylamine Source: Aldrich Chemical Company Structure: NH2—C—C—CH3 H2 H2 3 It is distilled over CaH2 before use. 7. N-ethyl N-benzyl amine Source: Aldrich Chemical Company Structure:
Figure imgf000009_0002
used without further purification. 8. Methanol Source: Burdick and Jackson
Structure CH3OH
used without further purification. 9. Molecular Sieve (Size 3A) Source: EM Science 1. Model Reaction Molecular Weight: 1108 g/mole Reaction scheme:
Figure imgf000010_0001
Room CH3OH (Methanol,
H3CO-vww O— C IJ -C-C-N-C-C-N-C-C-C II — Q w w* H H2 π H H2 π π H OCH3
Procedure : Ethylene diamine (0.5714 g, 0.9525*10"3 moles) and PEGMA (1 gm, 1.905*10"3) and 3 mL of methanol are added to a clean and dry vial. The vial is capped and allowed to shake for 2 hours. Methanol is removed under vacuum at room temperature. The product is a sticky solid. Spectral Data :
IR: cm"1
3514.3, 2873.2, 1723.8, 1656.3, 1462.3, 1449.6, 1407.5, 1344.2,
1293.6 1276.7, 1243.0, 1192.4, 1108.0 LH NMR: (δ ppm) in CD3OD
2.52(m), 2.69(s), 2.84(t), 3.35(s), 3.53 (m) , 3.62(d), 3.66(m),
4.70(t)
13C NMR: (δ ppm)
33.33, 34.76, 45.67, 52.10, 53.92, 59.08, 62.17, 64.68, 70.05, 71.31, 72.91, 73.64
2. PAMAM Dendrimer (Generation = 0) -PEGMA (1/4 equivalent) conjugate
Molecular weight: 1041 g/mole
Reaction scheme:
Figure imgf000012_0001
Room Temperature CH 3OH (Methanol, Solvent)
NH,
H2N— Q— i NH -
Figure imgf000012_0002
Procedure: 1 mL of G0 solution (20 % solution in methanol) is taken in a clean dry vial. PEGMA (0.0254g, 0.00019 moles) is added to the vial along with the 2 mL methanol. The vial is capped tightly and allowed to shake for about 3 hours. The methanol is removed after the reaction is over. The product is a sticky solid. Spectral data
XH NMR: (δ ppm) in D20
2.42(t), 2.60(s), 2.65(t), 2.70(t), 3.24(t), 3.28(s), 3.40(s),
3.62(m), 3.65(s), 3.80(m), 4.24(t).
13C NMR: (δ ppm)
32.59, 36.41, 36.65, 40.96, 41.13, 45.07, 46.97, 48.80, 49.06,
49.96, 58.01, 60.30, 69.41, 69.54, 69.63, 70.94, 71.69, 174.92,
175.03, 175.21, 175.30, 175.97, 180.62, 180.70
3. PAMAM Dendrimer (Generation = 0) - PEGMA (4 equivalents] conjugate
Molecular weight : 2613 g/mole
Reaction Scheme :
Figure imgf000014_0001
Hi—cHr- o J-CH2—CH— ∞H : Generation, G Q PEGMA n
Room Temperature CH 3OH (Methanol, Solvent)
Figure imgf000014_0002
Procedure: 1 mL of Go solution (20 % solution in methanol) is taken in a clean dry vial. PEGMA (0.1016g, 0.00076 moles) is added to the vial along with the 2 mL methanol. The vial is capped tightly and allowed to shake for about 3 hours. The methanol is removed after the reaction is over. The product is a sticky solid. Spectral Data : XH NMR: (δ ppm) in D20
2.42 (t), 2.59(m), 2.70(t), 2.82(1-0, 3.29(t), 3.37(s), 3.62 (m) , 3.69(s), 3.80(m), 4.30(d) 13C NMR: (δ ppm)
21.65, 33.90, 34.52, 34.90, 38.52, .39.21, 45.70, 47.05, 49.29, 49.29, 49.68, 50.82, 51.19, 51.35, 52.28, 53.26, 53.88, 54.01, 54.72, 54.90, 56.92, 60.55, 62.84, 66.43, 70.93, 71.95, 72.08, 72.17, 73.49, 74.23, 176.60, 176.70, 177.06, 177.51, 177.83, 180.58.
4. PAMAM Dendrimer (Generation=0) - PEGMA (2 equivalents) - Benzaldehyde (3 equivalents) Molecular weight: 1305 g/mol
Reaction scheme :
Figure imgf000016_0001
-L cHr- CH- O -C HΓ-CHT- OCHS Generation, G 0 PEGMA n +
Figure imgf000016_0002
Benzaldehyde
Room Temperature CH3OH (Methanol, Solvent)
Figure imgf000016_0003
Procedure: 2mL of G0 and Benzaldehyde (0.24603 g, 0.00232107 moles) are placed in a clean dry vial, capped tightly and allowed to shake for about 24 hours. PEGMA (0.40612 g, 0.000774 moles) is added thereafter. The vial is allowed to shake for about 100 hours. The methanol is removed under vacuum at room temperature. The product is a sticky solid. Spectral data IR: cm"1 1107.4, 1196.6, 1273.6, 1293.9, 1350.6, 1407.3, 1451.9, 1723.5, 2865.6
XH NMR: (δ ppm) in CD3OD
2.30 (m) , 2.45 (m) , 2.60 ( ) , 2.70 (m) , 2.95 (m) , 3.34 (s), 3.62 (s), 4.20 (d), 7.40 (m) , 7.75 (m) , 7.795 (m) , 8.30 (d) 13C NMR: (δ ppm)
33.50, 33.63, 33.83, 38.47, 39.11, 40.74, 41.02, 45.14, 50.41, 50.85, 51.76, 52.27, 53.65, 59.07, 60.99, 62.14, 64.76, 71.27, 71.30, 71.45, 72.87, 73.61, 128.78, 129.40, 129.78, 130.26, 131.40, 132.21, 137.01, 165.20, 173.86, 174.09, 174.63, 174.95
5. PAMAM Dendrimer (Generation=0) - PEGMA (2 equivalents) - Acetophenone (3 equivalents) Molecular weight: 1347 g/mole
Procedure: l L of G0 and Acetophenone (0.1395 g, 0.0021161 moles) are placed in a clean dry vial. 2 scoops of molecular sieve were added to the vial. It was capped tightly and allowed to shake for about 24 hours. PEGMA (0.4062 g, 0.000774 moles) was added thereafter and allowed to shake for about 100 hours. The methanol was removed under pump at room temperature. The product is a sticky solid. Spectral data IR: cm"1
1036.2, 1107.4, 1139.8, 1196.6, 1251.3, 1273.6, 1295.9, 1348.6,
1407.3, 1447.3, 1597.8, 1634.3, 1658.6, 1723.5, 2865.6, 3514.1.
XH NMR: (δ ppm) in D20 2.22(s), 2.43(t), 2.57(m), 2.65(s), 2.70(m), 2.80(d), 3.28(t),
3.30(m), 3.35(s), 3.38(s), 3.70(3), 3.63 (m) , 7.46(q), 7.55(t),
7.69(m) , 7.98 (d) .
13C NMR: (δ ppm)
17.65, 21.76, 28.42, 28.92, 32.90, 34.05, 35.21, 36.11, 41.12, 42.21, 42.62, 42.98,46.12, 47.60, 49.61, 51.51, 52.61, 54.87,
57.03, 60.70, 63.00, 72.10, 72.23, 73.63, 74.37, 131.18,
131.47, 136.81, 139.00, 165.00, 177.50, 177.89, 177.80, 182.64.
6. Model Reaction a. Schiff base formation by the reaction between propylamine and Acetophenone; Molecular weight: 161g/mol Structure scheme :
Figure imgf000019_0001
Procedure: Acetophenone (1.006 g, 0.00779 moles) and Propylamine (2.510 g, 0.0425 moles) are added in a clean dry vial. About 4 scoops of warm molecular sieve are added to the vial and capped tightly. The vial is allowed to shake for about 96 hours. The solution is collected using the solvent CH2C12 and dried over MgS04. The solution is filtered through celite and dried under vacuum at room temperature. Spectral data
IR: cm"1 (intensity)
1027.04, 1074.89, 1180.02, 1376.10, 1446.25, 1492.57, 1578.19, 1634.03, 2872.57, 2930.89, 2958.92, 3024.00, 3058.14, 3078.24. MS: m/e (% of base) 162, 16, 160, 146, 132, 104, 91 (100%), 77 XH NMR: (δ ppm) in CDC13
1.01 (t), 1.78 (q), 2.20 (s) , 3.42 (t) , 7.34 (m) , 7.76 (m) . 13C NMR (CD3OD) : (δ ppm) 12.07, 15.31, 24.05, 29.05, 53.85, 54.99, 77.00, 125.61, 128.02, 129.10, 141.34, 164.67, 165.41, 168.22. b. Reduction of imine:
Molecular weight : 163 g/mole Reaction scheme :
Figure imgf000020_0001
Procedure: Product (1.348g, 0.008373 moles) of the above reaction is placed in a clean dry vial. NaBH3CN (0.350g, 0.00557moles) was taken in a clean dry vial and about 1.5 mL of methanol is added to that in order to make a clear solution. The solution is added to the reaction vial and capped tightly. The vial is allowed to shake for about 72 hours at room temperature. After 72 hours about 6(N) HC1 is added drop by drop until the pH is <2. Then the solution is brought to a pH of >10 with 10% NaOH solution. The opaque solution is then dried over MgS0, filtered through celite and dried over vacuum at room temperature. Spectral data IR: cm"1 (intensity) 1027.53, 1071.61, 1286.90, 1370.64, 1450.87, 1492.02, 1601.13, 2871.74, 2931.94, 2959.54, 3025.48, 3062.21. MS: m/e (% of base) 163 , 162 , 148 , 134 , 105 ( 100% ) , 77 lH NMR: (δ ppm) in CD3OD 0.94 (t), 3.66 (d), 2.69 (m) , 2.88 (m) , 3.34 (s), 4.35 (q) ,
4.93 (s) , 7.47 (m) .
13C NMR: (δ ppm) in CD3OD
9.87, 18.30, 19.36, 58.14, 127.15, 129.05, 129.16, 136.54.
c. N-EthylBenzyl amine-PEGMEA conjugates: Structure: Reactive
θ — CH2 — CH. OCH, n
Figure imgf000021_0001
8
Procedure: PEGMA (lg, 0.00191 moles), N-ethyl benzylamine (0.258 g, 0.00191 moles) and methanol are placed in a clean dry vial. The vial is capped tightly and allowed to shake for about 72 hours. The methanol is evaporated off under vacuum at room temperature. Spectral data H NMR: (δ ppm) in CD3OD
1.03 (t), 2.48 (q) , 2.77 (t) , 3.34 (s), 3.57 (m) , 3.82 (m) , 4.19 (t), 4.28 (t), 7.23 (m) , 7.29 (d) . 13C NMR: (δ ppm) in CD3OD
11.96, 32.91, 33.05, 48.06, 49.57, 52.02, 58.80, 59.08, 62.18, 64.66, 64.81, 71.32, 71.51, 72.92, 73.64, 128.06, 129.21, 130.12, 140.15, 167.44, 174.10, 174.63.
7. Pegylation of reduced PAMAM Dendrimer (Generation=0) - Benzaldehyde (4 equivalents) conjugates: Molecular weight: 2977 g/mole Reaction scheme:
Figure imgf000022_0001
b.
H
Figure imgf000023_0001
Figure imgf000024_0001
Room Temperature
Figure imgf000024_0002
Procedure: 2mL (7.737X 10" moles) of Go and benzaldehyde (0.328 g, 3.095X10"3 moles) are placed in a clean dry vial. About 2.5 mL methanol is added to the reaction vial, capped tightly and allowed to shake for about 24 hours. Sodium cyanohydridoborate (0.129 g, 2.058X10"3 moles) is taken in a clean dry vial and a clean solution with minimum volume (about lmL) of methanol is made. The solution is added to the reaction vial and capped tightly. The vial is allowed to shake for 24 hours more. Thereafter PEGMA (0.8124 g, 1.547X10"3 moles) is added and the vial is shaken for another 60 hours. Then concentrated HCl (about 6 N) is added drop by drop until the pH is <2. The acidic solution is kept for about 2 hours and then is brought up to a pH of >10 by the drop by drop addition of 10% NaOH solution and then dried over MgS0 and filtered through celite. The methanol is then evaporated under vacuum at room temperature. Spectral data: A. G0+ 4Benzaldehyde XR NMR: (δ ppm) in CD30D 2.23 (t), 2.35 (s), 2.59 (t) , 3.34 (s), 3.48 (t) , 3.66 (t) , 4.92 (s), 5.46 (s), 7.40 (m) , 7.53 (t) , 7.60 (t) , 7.63 (t) , 7.65 (t), 7.73 (m) , 7.84 (m) , 7.86 (t) , 8.25 (s), 10.00 (s) . 13C NMR: (δ ppm) in CD3OD
32.56, 34.24, 35.93, 39.10, 40.94, 42.80, 50.93, 51.784, 52.65, 53.58, 59.06, 59.11, 60.86, 61.05, 62.67, 62.73, 128.28, 128.62, 128.92, 129.02, 129.50, 129.59, 130.39, 130.72, 130.73, 130.95, 131.05, 131.15, 131.55, 131.63, 131.71, 133.08, 133.18, 133.28, 134.35, 134.45, 136.83, 136.93, 137.07, 163.94, 166.04, 174.66, 192.77, 192.83, 192.90, 195.09, 195.15, 195.21. B. G0+ 4Benzaldehyde+ NaBH3CN XR NMR: (δ ppm) in CD3OD
2.54 (m) , 2.53 (m) , 2.73 (m) , 2.86 (m) , 3.34 (s) , 3.38 (s) , 3.59 (s), 3.76 (s), 4.622 (s) , 4.89 (s) , 5.01 (t) , 5.11 (m) , 7.34 (m) , 7.54 (m) , 7.76 (m) , 8.35 (m) . 13C NMR: (δ ppm) in CD3OD
34.67, 38.41, 39.85, 42.01, 42.96, 47.43, 51.23, 52.33, 53.71, 54.15, 57.10, 59.42, 65.19, 116.96, 120.39, 127.92, 128.13, 128.51, 128.76, 128.89, 129.26, 129.43, 129.66, 129.87, 135.37, 136.62, 139.00, 140.63, 142.63, 174.51, 174.60, 175.02, 175.09, 175.16.
C. G0+ 4Benzaldehyde+ NaBH3CN+PEGMEA IR: cm"1 (intensity) 11091, 1198.77, 1249.93, 1293.76, 1350.91, 1452.86, 1542.64, 1564.72, 1630.09, 1658.20, 1736.78, 2871.20. XH NMR: (δ ppm) in DMSO-d6
2.15 (s), 2.42 (t), 2.49 (m) , 2.58 (s) , 2.60 (d) , 3.08 (d) , 3.15 (s), 3.22 (s), 3.41 (t) , 3.49 (s) , 3.53 (s), 4.47 (d) , 4.66 (t), 7.21 ( ), 7.29 (m) , 7.41 (m) , 7.54 (m) , 7.88 (m) . 13C NMR: (δ ppm) in DMSO-d6
31.86, 32.04, 33.21, 36.48, 48,61, 48.86, 49.79, 51.25, 52.30, 57.57, 58.08, 60.20, 62.87, 69.60, 69.80, 71.30, 72.35, 126.43, 126.63, 126.81, 127.31, 127.51, 128.70, 128.17, 128.51, 128.71, 139.32, 142.57, 171.21, 172.52. 8. Pegylation of reduced PAMAM Dendrimer (Generation=0) Acetophenone (4 equivalents) conjugates: Molecular weight: 3033 g/mole Reaction scheme:
Figure imgf000027_0001
12
Figure imgf000027_0002
12
Figure imgf000028_0001
Room Temperature
Figure imgf000028_0002
14
Procedure: 2mL (7.737X 10 ,- moles) of G0 and acetophenone (0.372 g, 3.095X10"3 moles) are placed in a clean dry vial. About 2.5 mL methanol is added to the reaction vial along with 3 scoops of molecular sieve (3A) , capped tightly and allowed to shake for about 24 hours. Sodium cyanohydridoborate (0.129 g, 2.058X10"3 moles) is taken in a clean dry vial and a clean solution with minimum volume (about lmL) of methanol is made. The solution is added to the reaction vial and capped tightly. The vial is allowed shake for 72 hours more. Thereafter PEGMA (0.8124 g, 1.547X10"3 moles) is added and the vial is allowed to shake for another 120 hours. The solution turns to a pink color. Then concentrated HCl (about 6 N) is added drop by drop until the pH is <2. The acidic solution is kept for about 2 hours and then is brought up to a pH of >10 by the drop by drop addition of 10% NaOH solution, dried over MgS04 and filtered through celite. The methanol is then evaporated under vacuum at room temperature. Spectral data:
A. GQ+4 Acetophenone: lE NMR: (δ ppm) in CDC13
2.20 (m), 2.65 (d) , 2.57 (t) , 2.62 (S) , 2.72 (t) , 3.22 (m) , 3.34 (m) , 3.49 (d) , 3.55 (t) , 7.07 (m) , 7.27 (s), 7.35 (m) , 7.47 (t), 7.59 (t), 7.72 ( ) , 7.98 (d) . 13C NMR: (δ ppm) in CDC13 16.19, 16.27, 34.03, 34.13, 34.31, 50.36, 50.68, 50.80, 51.38, 51.54, 51.69, 76.58, 77.42, 125.84, 126.56, 128.17, 129.62, 133.10, 140.83, 167.14, 167.32, 172.68, 172.77, 173.01.
B. Go+4 Acetophenone+ NaBH3CN l H NMR: (δ ppm) in CD3OD 1.37 (m) , 1.43 (d) , 2.36 (m) , 2.51 (t) , 2.60 (s) , 2.72 (m) , 2.85 (t), 3.27 ( ) , 3.30(m), 3.37 (s), 3.78 (m) , 7.22 ( ) , 7.31(m), 7.47 (m) , 7.49 (d) , 7.52 (d) , 7.58 (d) , 7.98 (d) , 8.01 (t) . 13C NMR: (δ ppm) in CD3OD
23.99, 25.62, 34.57, 39.96, 41.10, 42.918, 47.66, 49.85, 51.19, 52.29, 59.12, 70.81, 126.42, 127.77, 128.05, 128.15, 129.24, 129.56, 145.93, 147.80, 174.99, 175.21.
C. Go+4 Acetophenone+ NaBH3CN +PEGMEA
IR: cm"1 (intensity)
1101.38, 1199.40, 1249.53, 1288.77, 1350.85, 1452.80, 1580.67,
1630.78, 1657.81, 1736.70, 2873.04.
1 H NMR: (δ ppm) in DMSO-d6 1.18 (d) , 1.28 (d), 2.15 (s) , 2.36 (t) , 2.49 (t) , 2.56 (s),
2.65 (t), 3.03 (t), 3.14 (d) , 3.21 (s) , 3.40 (t) , 3.48 (s) ,
3.54 (m) , 4.22 (q) , 4.68 (t) , 7.64 (d) , 7.27 (m) , 7.87 (m) ,
8.07 (m) .
13C NMR: (δ ppm) in DMSO-d6 24.64, 32.11, 33.33, 36.72, 46.76, 48.62, 48.98, 49.98, 49.98,
51.29, 52.34, 57.42, 58.12, 60.22, 69.64, 69.84, 71.34, 72.38,
125.35, 126.54, 127.48, 128.03, 128.23, 146.24, 171.35, 171.43,
172.55. Analysis of spectral data especially the XH NMR analysis of the pegylated PAMAM dendrimer revealed the fact that the double bond of PEGMA is allowed to react with the terminal amine groups of PAMAM dendrimer qualitatively. However, it was observed that the desired product, 3, is not apparently formed. Detailed analysis of NMR spectra suggested that although 3 forms first, it is rapidly converted into 15 in the presence of solvent methanol. A considerable amount of compound 15 gets converted back to 3 during the removal of methanol at the completion of the reaction. Moreover, both 3 and 15 are unstable in presence of water and are hydrolyzed rapidly to 16. The mechanism is believed to be a series of substitution reactions described below. The intra molecular hydrogen bond appears to be playing an important role to facilitate above described reactions.
Figure imgf000031_0001
OCH,
III 1,1 CZZ^ Denotes H-bonding
Figure imgf000032_0001
-Methanol lfi Transesterification
Figure imgf000032_0002
15 Major
Major
Figure imgf000032_0003
17 An examination of the above scheme clearly demonstrates the proposition that the hydrogen atom on the nitrogen atom plays a pivotal role in the production of 15 and 17, instead of the desired product 3. Furthermore, it is suspected that the dendrimer molecule under investigation can speed up transesterification and hydrolysis processes readily. It is also suspected that trans-esterification and hydrolysis processes occurs for quarter as well as per pegylation. It is observed from the NMR spectrum that the intensity ratio of the peaks corresponding to the methoxy groups (OCH3) of methanol and compound 16 was changed with the time upon the addition of the water. When water is added to the pegylated PAMAM dendrimer, the changes of the intensity ratio of the absorbance correspond to methoxy groups of methanol and compound 16 can be monitored by 1H NMR spectrum. The ratio Vs time is noted as illustrated in Table 1 below. It is suspected from the data that rates of hydrolysis follow the same trend.
Table 1
Time(min) Ratio 1/Rato 7 0.48 2.1 19 0.47 2.13 22 0.51 1.97 27 0.65 1.54 31 0.41 2.44 35 0.57 1.74 39 0.66 1.51 44 0.53 1.87 48 0.67 1.5 52 0.76 1.3 56 0.69 1.44 61 0.71 1.41 67 0.71 1.4 6870 1.49 0.67 In order to circumvent the undesired reactions after pegylation, it was decided to block three of the four amine groups of PAMAM Dendrimer (Generation =0) to remove all the hydrogen attached to three primary residual nitrogen atoms. The remaining primary residual nitrogen atom can then be pegylated without any unwanted product. Benzaldehyde and acetophenone were used for above purpose (and almost any aldehyde or ketone can be used for this purpose and an aldehyde substituted cyclodextrin is believed to be especially useful in this respect) . As carbonyl group of benzaldehyde and acetophenone reacts with primary residual amine groups of dendrimer (Generation = 0) to form an imine. Reactions were carried out using little excess of such reagents. Analysis of the product by NMR indicated that the expected product 5 was formed along with the other side products. Blocking only three residual amine groups out of four was not achieved successfully and was complicated due to distribution of benzaldehyde and acetophenone molecules as well, since side products 9, 10 and others formed along with the expected product 5.
Hr-C r-OCH3
Figure imgf000035_0001
CHO
Benzaldehyde
Figure imgf000035_0002
CH2— CHr- O -hCH — CHτ~ OCH3 PEGMA
Figure imgf000036_0001
Acetophenone Room Temperature CH3OH (Methanol, Solvent)
Figure imgf000036_0002
10
Model reactions 6.a and 6.b show that formation of Schiff base followed by reduction of imine functional group can be achieved. The presence of absorbance at 1642 cm"1 confirm the formation of imine. On the other hand the absence of that absorbance in IR spectrum after reduction proved that imine functional group can be reduced. Furthermore, the secondary amine can be pegylated and form the desired product. All the four primary amine groups of dendrimer molecules (Generation = 0) can be converted to imine groups by reacting with carbonyl groups of, for example, benzaldehyde as well as acetophenone. The synthesis routes are shown in scheme 7 and 8 to remove the hydrogen atoms attached to residual primary amine groups. An examination of the spectra reveals the presence of absorbance corresponding to imine functional groups. Analysis of the products by IR and NMR indicates that all amine groups were converted to imines. The imine groups of products 9 and 12 can be reduced by selective reducing agent as dendrimer molecule contains acid amide groups which are very sensitive to reducing agents. Sodium cyanohydridoborate hydride is used for this specific purpose. An examination of 1H and 13C NMR spectra as well as IR spectra showed that the absence of the absorbance due to imine group. The absorbance of the reduced products 10 and 13 are identified. The hydrogen atoms of compounds 10 and 13 associated with four nitrogen atoms are pegylated in the following step. NMR analysis of the products indicates that compounds 10 as well as 13 were pegylated. The examination of spectral data showed the formation of alcohol of the corresponding aldehyde as side product as excess aldehyde was used for those reactions. In addition, Boron complex compound was formed during the reduction step. Most of the absorbance of the final product in 1H and 13C NMR spectra is identified with the help of 1H-1H and 1H-13C correlation spectra. It is noticed that reaction schemes 7 as well as 8 can not apparently be accomplished step by step. It is thought necessary that reduced compounds 10 and 13 to be worked up in order to remove unwanted Boron complex compounds after reduction of the imine. It is observed that reduced product can not be dissolved further in low boiling point solvent such as methanol once solvent was removed after working up. This is apparently because of the formation of intra-molecular H-bonding after the solvent is removed at room temperature under vacuum. This kind of intra-molecular H-bond makes reduced compounds 10 and 13 reluctant to form further inter-molecular H-bonding with solvent. For the sake of simplicity in isolating the product, a low boiling solvent is probably the best choice for this reaction. Therefore, a modified procedure is developed in order to avoid such solubility problem. The problem associated with solubility is avoided by pegylating compounds 10 and 13 before work up. The analysis of 1H NMR indicated that double bonds of PEGMEA are reacted with secondary amine groups of compounds 10 and 13. The reactions are carried and dendrimers are pegylated first without working it up. Spectral data showed that dendrimer is successfully pegylated. It can be problematic to work up the pegylated products 11 and 14 as cleavage or hydrolysis could have been possible during work up. However, examination of the 1H, 13C NMR spectra showed that the PEG molecules remain unchanged after treatment with strong acid and base. It is observed that delicate ether and carbonyl moieties of pegylated products 11 and 14 are neither cleaved nor hydrolyzed. Gel electrophoresis of compound 11 and 14 along with different generation of dendrimer (ladder) are carried out to confirm the formation of compounds 11 and 14. The migration of 10 and 11 is slower than that of 13 and 14 and is explained on the basis of steric effects. Aldehyde substituted beta cyclodextrins are especially useful in the instant invention to block primary amines. For example, an amine terminated polyethylene glycol can be reacted with an aldehyde substituted beta cyclodextrin at room temperature in aqueous sodium cyanohydridoborate to couple the cyclodextrine to the polyethylene glycol via a nitrogen atom to form a pegylated cyclodextrine adduct. Then, the pegylated cyclodextrine adduct can be reacted with the polyoxyalkylene acrylate to form a cyclodextrin adduct that is further pegylated. Chitosan can be reacted with an aldehyde substituted beta cyclodextrin at room temperature in aqueous sodium cyanohydridoborate to block the primary amines of the chitosan followed by reaction with the polyoxyalkylene acrylate to form a pegylated and beta cyclodextrin substituted chitosan.
Peptides, polypeptides and proteins containing primary amines can be reacted with an aldehyde substituted beta cyclodextrin at room temperature in aqueous sodium cyanohydridoborate to block the primary amines of the peptide, polypeptide or protein followed by reaction with the polyoxyalkylene acrylate to form a pegylated and beta cyclodextrin substituted peptide, polypeptide or protein. As a specific example, the following scheme can be used in the instant invention to first convert the primary amines of poly-L-arginine to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate. A 3 necked, 25-mL, round- bottomed, flask is fitted with nitrogen inlet, a condenser with drying tube, a rubber septum, and a magnetic stir bar. The following ingredients are added to the flask: poly-L-arginine hydrochloride (5mg, 0.663μmol), beta cyclodextrin monoaldehyde
(58.0mg, 51.2μmol), 2-mL of deionized water and 1-mL sodium hydroxide solution (0.2586M). Immediately after addition of the base, sodium cyanoborohydride (8.6mg, 0.137mmol) is added. The reaction mixture is stirred for 72 hours at room temperature. Half the reaction mixture (1.5-mL) is then removed and precipitated in 5-mL acetone for analysis to confirm the desired reaction. The remainder of the reaction mixture is mixed with 1-mL of poly (ethylene glycol) methyl ether acrylate aqueous solution (0.0286M). The reaction mixture is then stirred for an additional 72 hours at room temperature and then precipitated in 10-mL of acetone and centrifuged to yield a white solid that is dried under vacuum overnight. Analysis of the white solid confirms the desired formation of a pegylated beta cyclodextrin-poly-L-arginine conjugate. As a specific further example, the following scheme can be used in the instant invention to first convert the primary amines of poly-L-lysine to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate. A 3 necked, 25-mL, round - bottomed, flask is fitted with a nitrogen inlet, a condenser with drying tube, a rubber septum and a magnetic stir bar. The following ingredients are added to the flask: poly-L-lysine hydrochloride (8mg, 0.5μmol), beta cyclodextrin monoaldehyde
(55.0mg, 48.5μmol), 2-mL of deionized water and 1-mL sodium hydroxide solution (0.0485M). Immediately after addition of the base, sodium cyanoborohydride (8.1mg, 0.129mmol) is added. The reaction mixture is then stirred for 72 hours at room temperature. Half the reaction mixture (1.5-mL) is then removed and precipitated in 5-mL acetone for analysis to confirm the production of the desired product. To the remainder of the reaction mixture, 0.5-mL poly (ethylene glycol) methyl ether acrylate aqueous solution (0.0502M) is added. The reaction mixture is then stirred for an additional 72 hours at room temperature and then precipitated in 10-mL of acetone and centrifuged to yield a white sold that was dried under vacuum overnight. Analysis of the white solid confirms the desired formation of a pegylated beta cyclodextrin-poly-L-lysine conjugate. As a specific additional example, the following scheme can be used in the instant invention to first convert the primary amines of Chitosan to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate. A 3 necked, 25-mL, round- bottomed, flask is fitted with a nitrogen inlet, a condenser with drying tube, a rubber septum and a magnetic stir bar. The following ingredients are added to the flask: low molecular weight chitosan (50mg, 0.185mmol) dissolved in 15-mL of 0.1M hydrochloric acid, 2.00g of beta-glucero-phosphate dissolved in 4-mL of deionized water, beta cyclodextrin monoaldehyde (421mg, 0.370mmol) and sodium cyanoborohydride (33 mg, 0.525mmol). The reaction mixture is then stirred for 72 hours at room temperature. 14-mL of the reaction mixture is removed for analysis to confirm the production of the desired product. To the remainder of the reaction mixture, 4.50-mL poly (ethylene glycol) methyl ether acrylate aqueous solution (0.0220M) is added. The reaction mixture is then stirred for and additional 72 hours at room temperature. The resulting solution is then lyophilized to yield a white fibrous solid. The solid is then washed with acetone using a soxhlet and dried under vacuum overnight. Analysis of the solid confirms the formation of the desired pegylated beta cyclodextrin-chitosan conjugate. As a yet further specific additional example, the following scheme can be used in the instant invention to pegylate glutathione. Glutathione (0.153 g, 0.0005 mole), poly (ethylene glycol) methyl ether acrylate (0.225 g, 0.0005 mole) are dissolved in 4 mL of a buffer solution (pH= 5.8) in a screw cap vial. The clear aqueous solution is allowed to mix on a tabletop shaker for 3h at room temperature. The entire reaction mixture is lypholized to produce the desired pegylated glutathione. The following scheme can be used in the instant invention for the pegylation of proteins: (a) the protein is dissolved or dispersed in 0.1M bicarbonate buffer, pH 9.1 (the concentration of proteins as a rule can be measured from their extinction coefficients at 280 nm) ; (b) mPEG acrylate solutions are prepared at various concentrations in 0.1M bicarbonate buffer, pH 9.1; (c) a known volume of the protein solution is mixed with the mPEG solution in various vials to yield various amino / mPEG ratios; (d) samples are incubated under defined temperatures and times with appropriate control tubes; and (e) after reaction, the reaction mixture is subjected to native gel electrophoresis in 10% polyacrylamide gels (protein staining, as a rule, is performed with Coomassie Blue). As a final additional specific example, the following scheme can be used in the instant invention to first convert the primary amines of a PAMAM dendrimer to secondary amines by the addition of a cyclodextrin to the amine group and then pegylation with a polyethylene glycol acrylate. A 3 necked, 25-mL, round- bottomed, flask is fitted with a nitrogen inlet, a condenser with drying tube, a rubber septum and a magnetic stir bar. The following ingredients are added to the flask: PAMAM generation 0 dendrimer (160mg, 0.310mmol) dissolved in 1- mL deionized water, beta cyclodextrin monoaldehyde (1.5010g, 1.32mmol) dissolved in 15-mL of deionized water and sodium cyanoborohydride (229.6mg, 3.65mmol). The reaction mixture is then stirred for 72 hours at room temperature. 6-mL of the reaction mixture is then removed and precipitated in methanol to confirm the production of the desired product. To the remainder of the reaction mixture, 9-mL poly (ethylene glycol) methyl ether acrylate aqueous solution (0.0880M) are added. The reaction mixture is then stirred for an additional 72 hours at room temperature and then precipitated in 10-mL of acetone and centrifuged to yield a white sold that was dried under vacuum overnight. Analysis confirms the desired production of pegylated beta cyclodextrin PAMAM dendrimer conjugate. Thus, it should be appreciated that in the instant invention any compound containing an amine group can be reacted with the polyoxyalkylene acrylate to form a conjugate comprising a polyoxyalkylene sub-structure. Furthermore, when the amine group is a primary amine, then it may be necessary (such as in the case of a PAMAM dendrimer) as a preliminary step to "block" the primary amine (s), as discussed above in detail, by reaction of such primary amine (s) with an aldehyde or ketone followed by conversion of the resulting imine to a secondary amine. Many drug compounds contain amine group (s) and it should be understood that the instant invention is an excellent means of converting such drugs to a polyoxyalkylene conjugate of the drug. The term "polyoxyalkylene" is defined in the above referenced US Patent 6,280,745, herein fully incorporated by reference, and includes polyethylene glycol, polypropylene glycol, as well as block and random polyethylene glycol/polypropylene glycol co-polymers. Although acrylate terminated polyethylene glycols are commercially available, acrylate terminated polyethylene glycol can be prepared, for example, by reacting a monomethoxy polyethylene glycol with acryloyl chloride or, for example, with methacroloyl chloride. The molecular weight of the polyoxyalkylene sub-structure of the instant invention can be tailored so that the conjugate has desired properties such as solubility characteristics that are more compatible with the biologic system. In many cases, the preferred molecular weight of the polyoxyalkylene sub- structure of the instant invention will be in the range of from about 500 to about 5000 grams per mole. In addition to reactions with amines, the acrylate terminated polyoxylakylene of the instant invention also can be reacted with a terminal sulfur (sulfide) group (s) of a biologically active compound to produce novel compounds. For example an aqueous buffered (pH=5.8) solution of glutathione can be pegylated at room temperature by a two hour reaction with the acrylate terminated polyethylene glycol of the instant invention. Polycysteine can be similarly pegylated. The process of the instant invention produces novel compounds that, as expected, maintain their biological activity. For example, bovine erythrocyte carbonic anhydrase (CAB) pegylated with mPEG acrylate at room temperature in a pH 9.1 aqueous buffer (mole ratio of CAB to mPEG acrylate of 1:8; 1:2 and 8:1) maintains its biological activity. As a further example, hen egg white lysozyme (HEWL) pegylated with mPEG acrylate at room temperature in a pH 9.1 aqueous buffer (mole ratio of HEWL to mPEG acrylate of 1:2 and 8:1) also maintains its biological activity.

Claims

WHAT IS CLAIMED IS: 1. A compound corresponding to the formula: formula 1 R2 R30 I I II Ri-N-CH2-C-C-0-R-0-R5 where Rj. is an organic radical where R2 is H or an organic radical where R3 is H or an organic radical where R is a polyoxyalkylene radical and where R5 is an organic radical or H.
2. The compound of Claim 1, wherein Rx is derived from a dendrimer. 3. The compound of Claim 1, wherein Ri is selected from the group consisting of a group derived from a peptide or a protein.
4. The compound of Claim 1, wherein R is derived from polyethylene glycol.
5. The compound of Claim 2, wherein R2 is derived from a cyclodextrin, wherein R3 is H, wherein R is derived from polyethylene glycol and wherein R5 is CH3.
6. The compound of Claim 3, wherein R2 is derived from a cyclodextrin, wherein R3 is H, wherein R is derived from polyethylene glycol and wherein R is CH3.
7. The compound of Claim 3, wherein R2 is H, wherein R3 is H, wherein R is derived from polyethylene glycol and wherein R5 is CH3.
8. A compound corresponding to the formula: formula 2 R70 I II
R6-S-CH2-C-C-0-R8-0-R9 where R6 is an organic radical where R is H or an organic radical where Re is a polyoxyalkylene radical and where R9 is an organic radical or H.
9. The compound of Claim 8, wherein R6 is selected from the group consisting of a group derived from a peptide or a protein.
10. The compound of Claim 8, wherein R8 is derived from polyethylene glycol.
11. The compound of Claim 9, wherein R is H, wherein Re is derived from polyethylene glycol and wherein R9 is CH3.
12. A method for preparing a compound corresponding to the formula : formula 1 R2 R30 I I tl R1-N-CH2-C-C-0-R4-0-R5 where Ri is an organic radical where R2 is H or an organic radical where R3 is H or an organic radical where R4 is a polyoxyalkylene radical and where R5 is an organic radical or H, comprising the step of: reacting A with B, wherein A is Rι-N-R2 and wherein R30 I II
B is C=C-C-0-R4-0-R5.
13. The process of Claim 12, wherein Ri is derived from a dendrimer.
14. The process of Claim 12, wherein Ri is selected from the group consisting of a group derived from a peptide or a protein.
15. The process of Claim 12, wherein R is derived from polyethylene glycol.
16. The process of Claim 13, wherein R2 is derived from a cyclodextrin, wherein R3 is H, wherein R4 is derived from polyethylene glycol and wherein R5 is CH3.
17. The process of Claim 14, wherein R2 is derived from a cyclodextrin, wherein R3 is H, wherein R4 is derived from polyethylene glycol and wherein R5 is CH3.
18. The process of Claim 14, wherein R2 is H, wherein R3 is H, wherein R4 is derived from polyethylene glycol and wherein R5 is CH3.
19. A method for preparing a compound corresponding to the formula: formula 2 R70 I K
R6-S-CH2-C-C-0-R8-0-R9 where R6 is an organic radical where R is H or an organic radical where R8 is a polyoxyalkylene radical and where R9 is an organic radical or H, comprising the step of: reacting D with E, wherein D is R6-S and wherein R70 t II
E is C=C-C-0-R8-0-R9.
20. The compound of Claim 19, wherein R6 is selected from the group consisting of a group derived from a peptide or a protein.
21. The compound of Claim 19, wherein R8 is derived from polyethylene glycol.
2. The compound of Claim 20, wherein R7 is H, wherein R8s derived from polyethylene glycol and wherein R9 is CH3.
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