WO2005095618A3 - Plants with reduced activity of the starch phosphorylating enzyme phosphoglucan, water dikinase - Google Patents

Plants with reduced activity of the starch phosphorylating enzyme phosphoglucan, water dikinase Download PDF

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Publication number
WO2005095618A3
WO2005095618A3 PCT/EP2005/002450 EP2005002450W WO2005095618A3 WO 2005095618 A3 WO2005095618 A3 WO 2005095618A3 EP 2005002450 W EP2005002450 W EP 2005002450W WO 2005095618 A3 WO2005095618 A3 WO 2005095618A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
starch
plant cells
relates
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2005/002450
Other languages
French (fr)
Other versions
WO2005095618A2 (en
Inventor
Claus Frohberg
Oliver Koetting
Gerhard Ritte
Martin Steup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience AG
Original Assignee
Bayer CropScience AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer CropScience AG filed Critical Bayer CropScience AG
Priority to DK05715846.1T priority Critical patent/DK1725666T3/en
Priority to EP05715846A priority patent/EP1725666B1/en
Priority to CA2557843A priority patent/CA2557843C/en
Priority to US10/591,540 priority patent/US7919682B2/en
Priority to AT05715846T priority patent/ATE541042T1/en
Publication of WO2005095618A2 publication Critical patent/WO2005095618A2/en
Publication of WO2005095618A3 publication Critical patent/WO2005095618A3/en
Anticipated expiration legal-status Critical
Priority to US13/033,495 priority patent/US8257502B2/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B31/00—Preparation of derivatives of starch
    • C08B31/02—Esters
    • C08B31/06—Esters of inorganic acids
    • C08B31/066—Starch phosphates, e.g. phosphorylated starch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1294—Phosphotransferases with paired acceptors (2.7.9)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a starch phosphorylating OKl protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. The OKl protein acts as a phosphoglucan, water dikinase (PWD) which predominately phosphorylates the C3 position. Genetically modified plants in which the expression of PWD is reduced show a starch excess phenotype. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules, which ar suitable for manufacturing plants according to the invention.
PCT/EP2005/002450 2004-03-05 2005-03-04 Plants with reduced activity of the starch phosphorylating enzyme phosphoglucan, water dikinase Ceased WO2005095618A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DK05715846.1T DK1725666T3 (en) 2004-03-05 2005-03-04 Plants with reduced activity for the starch phosphorylation enzyme phosphoglucan-water dikinase
EP05715846A EP1725666B1 (en) 2004-03-05 2005-03-04 Plants with reduced activity of the starch phosphorylating enzyme phosphoglucan, water dikinase
CA2557843A CA2557843C (en) 2004-03-05 2005-03-04 Plants with reduced activity of a starch phosphorylating enzyme
US10/591,540 US7919682B2 (en) 2004-03-05 2005-03-04 Plants with reduced activity of a starch phosphorylating enzyme
AT05715846T ATE541042T1 (en) 2004-03-05 2005-03-04 PLANTS WITH REDUCED ACTIVITY OF THE STARCH PHOSPHORYLATING ENZYME PHOSPHOGLUCAN-WATER DIKINASE
US13/033,495 US8257502B2 (en) 2004-03-05 2011-02-23 Plants with reduced activity of a starch phosphorylating enzyme

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US55002204P 2004-03-05 2004-03-05
EP04090088.8 2004-03-05
EP04090088 2004-03-05
US60/550,022 2004-03-05
EP04090121.7 2004-03-29
EP04090121 2004-03-29
EP04090203 2004-05-21
EP04090203.3 2004-05-21
EP04090484 2004-12-09
EP04090484.9 2004-12-09

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/591,540 A-371-Of-International US7919682B2 (en) 2004-03-05 2005-03-04 Plants with reduced activity of a starch phosphorylating enzyme
US13/033,495 Division US8257502B2 (en) 2004-03-05 2011-02-23 Plants with reduced activity of a starch phosphorylating enzyme

Publications (2)

Publication Number Publication Date
WO2005095618A2 WO2005095618A2 (en) 2005-10-13
WO2005095618A3 true WO2005095618A3 (en) 2006-03-02

Family

ID=34981782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/002450 Ceased WO2005095618A2 (en) 2004-03-05 2005-03-04 Plants with reduced activity of the starch phosphorylating enzyme phosphoglucan, water dikinase

Country Status (6)

Country Link
US (2) US7919682B2 (en)
EP (1) EP1725666B1 (en)
AT (1) ATE541042T1 (en)
CA (1) CA2557843C (en)
DK (1) DK1725666T3 (en)
WO (1) WO2005095618A2 (en)

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US9574201B2 (en) 2010-06-09 2017-02-21 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
US9593317B2 (en) 2010-06-09 2017-03-14 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

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