WO2005118608A3 - 2’-nitrobenzyl-modified ribonucleotides - Google Patents

2’-nitrobenzyl-modified ribonucleotides Download PDF

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Publication number
WO2005118608A3
WO2005118608A3 PCT/IB2005/002488 IB2005002488W WO2005118608A3 WO 2005118608 A3 WO2005118608 A3 WO 2005118608A3 IB 2005002488 W IB2005002488 W IB 2005002488W WO 2005118608 A3 WO2005118608 A3 WO 2005118608A3
Authority
WO
WIPO (PCT)
Prior art keywords
nitrobenzyl
modified ribonucleotides
ribonucleotides
disclsoed
nucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2005/002488
Other languages
French (fr)
Other versions
WO2005118608A2 (en
Inventor
Olaf Piepenburg
Derek L Stemple
Niall A Armes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TwistDix Inc
Original Assignee
TwistDix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2007514217A priority Critical patent/JP2008501679A/en
Priority to DE602005009693T priority patent/DE602005009693D1/en
Priority to EP05769767A priority patent/EP1753774B1/en
Priority to CA002569491A priority patent/CA2569491A1/en
Priority to US11/628,343 priority patent/US7759061B2/en
Priority to AU2005250239A priority patent/AU2005250239A1/en
Application filed by TwistDix Inc filed Critical TwistDix Inc
Publication of WO2005118608A2 publication Critical patent/WO2005118608A2/en
Publication of WO2005118608A3 publication Critical patent/WO2005118608A3/en
Anticipated expiration legal-status Critical
Priority to US12/803,261 priority patent/US8022194B2/en
Priority to US13/206,926 priority patent/US8299226B2/en
Priority to US13/658,023 priority patent/US20130045475A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclsoed nucleotides are also provided.
PCT/IB2005/002488 2004-06-02 2005-06-02 2’-nitrobenzyl-modified ribonucleotides Ceased WO2005118608A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE602005009693T DE602005009693D1 (en) 2004-06-02 2005-06-02 2 "-NITROBENZYL-MODIFIED RIBONUCLEOTIDE
EP05769767A EP1753774B1 (en) 2004-06-02 2005-06-02 2"-nitrobenzyl-modified ribonucleotides
CA002569491A CA2569491A1 (en) 2004-06-02 2005-06-02 2'-nitrobenzyl-modified ribonucleotides
US11/628,343 US7759061B2 (en) 2004-06-02 2005-06-02 2′-nitrobenzyl-modified ribonucleotides
AU2005250239A AU2005250239A1 (en) 2004-06-02 2005-06-02 2'-nitrobenzyl-modified ribonucleotides
JP2007514217A JP2008501679A (en) 2004-06-02 2005-06-02 2'-nitrobenzyl modified ribonucleotide
US12/803,261 US8022194B2 (en) 2004-06-02 2010-06-21 2′-nitrobenzyl-modified ribonucleotides
US13/206,926 US8299226B2 (en) 2004-06-02 2011-08-10 2-nitrobenzyl-modified ribonucleotides
US13/658,023 US20130045475A1 (en) 2004-06-02 2012-10-23 2-Nitrobenzyl-Modified Ribonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57690704P 2004-06-02 2004-06-02
US60/576,907 2004-06-02

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US11/628,343 A-371-Of-International US7759061B2 (en) 2004-06-02 2005-06-02 2′-nitrobenzyl-modified ribonucleotides
US12/803,261 Continuation US8022194B2 (en) 2004-06-02 2010-06-21 2′-nitrobenzyl-modified ribonucleotides

Publications (2)

Publication Number Publication Date
WO2005118608A2 WO2005118608A2 (en) 2005-12-15
WO2005118608A3 true WO2005118608A3 (en) 2006-04-27

Family

ID=35463420

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/002488 Ceased WO2005118608A2 (en) 2004-06-02 2005-06-02 2’-nitrobenzyl-modified ribonucleotides

Country Status (8)

Country Link
US (4) US7759061B2 (en)
EP (1) EP1753774B1 (en)
JP (3) JP2008501679A (en)
AT (1) ATE407939T1 (en)
AU (1) AU2005250239A1 (en)
CA (1) CA2569491A1 (en)
DE (1) DE602005009693D1 (en)
WO (1) WO2005118608A2 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7928207B2 (en) 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US8808988B2 (en) 2006-09-28 2014-08-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9206476B2 (en) 2005-12-21 2015-12-08 Roche Molecular Systems, Inc Sequencing and genotyping using reversibly terminating nucleotides

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2569491A1 (en) 2004-06-02 2005-12-15 Asm Scientific, Inc. 2'-nitrobenzyl-modified ribonucleotides
US8486717B2 (en) 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
US9874556B2 (en) 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
WO2015038978A1 (en) 2013-09-13 2015-03-19 Symbolics, Llc Lateral flow assays using two dimensional test and control signal readout patterns
US10351918B2 (en) 2013-10-30 2019-07-16 Green Life Biotech, Llc Pathogenesis quantification systems and treatment methods for citrus greening blight
JP2024518331A (en) 2021-04-29 2024-05-01 アボット・ラボラトリーズ High-throughput nucleic acid testing of biological samples

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor

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JPS58109498A (en) * 1981-08-24 1983-06-29 Agency Of Ind Science & Technol Oligoribonucleotide
JPS5836396A (en) * 1981-08-24 1983-03-03 Agency Of Ind Science & Technol Method for producing oligoribonucleotides
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US20010018514A1 (en) * 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
EP1159453B1 (en) * 1999-03-10 2008-05-28 ASM Scientific, Inc. A method for direct nucleic acid sequencing
JP2001288197A (en) * 2000-04-10 2001-10-16 Fuji Photo Film Co Ltd Fluorescent nucleotide
ATE356222T1 (en) * 2000-10-06 2007-03-15 Univ Columbia MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA
CN1348096A (en) * 2000-10-10 2002-05-08 栾国彦 Homogeneous specific nucleic acid detecting probe and its application method
CA2425663C (en) * 2000-10-11 2009-12-29 Applera Corporation Fluorescent nucleobase conjugates having anionic linkers
CA2476481C (en) 2002-02-21 2016-01-26 Asm Scientific, Inc. Recombinase polymerase amplification
EP3985125A1 (en) 2004-06-01 2022-04-20 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
CA2569491A1 (en) * 2004-06-02 2005-12-15 Asm Scientific, Inc. 2'-nitrobenzyl-modified ribonucleotides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136543A (en) * 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
METZKER M L ET AL: "TERMINATION OF DNA SYNTHESIS BY NOVEL 3'-MODIFIED-DEOXYRIBONUCLEOSIDE 5'-TRIPHOSPHATES", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 22, no. 20, 1994, pages 4259 - 4267, XP001068669, ISSN: 0305-1048 *
SHIN, SEUNGJIN ET AL: "Synthesis and photoaffinity labeling of 3'(2')-O-(p- azidobenzoyl )-ATP", JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY , 30(3), 211-215 CODEN: JBMBE5; ISSN: 1225-8687, 1997, XP009060719 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7919249B2 (en) 2003-06-30 2011-04-05 Roche Molecular Systems, Inc. 2′-Terminator nucleotide-related methods and systems
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US8163487B2 (en) 2003-06-30 2012-04-24 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
US7928207B2 (en) 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US9206476B2 (en) 2005-12-21 2015-12-08 Roche Molecular Systems, Inc Sequencing and genotyping using reversibly terminating nucleotides
US8808988B2 (en) 2006-09-28 2014-08-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
US9469873B2 (en) 2006-09-28 2016-10-18 Illumina, Inc. Compositions and methods for nucleotide sequencing

Also Published As

Publication number Publication date
CA2569491A1 (en) 2005-12-15
ATE407939T1 (en) 2008-09-15
EP1753774B1 (en) 2008-09-10
US8299226B2 (en) 2012-10-30
DE602005009693D1 (en) 2008-10-23
US8022194B2 (en) 2011-09-20
AU2005250239A1 (en) 2005-12-15
JP2008501679A (en) 2008-01-24
US20130045475A1 (en) 2013-02-21
US20110053154A1 (en) 2011-03-03
WO2005118608A2 (en) 2005-12-15
JP2012095665A (en) 2012-05-24
US20120040345A1 (en) 2012-02-16
US7759061B2 (en) 2010-07-20
JP2012157369A (en) 2012-08-23
US20080261208A1 (en) 2008-10-23
EP1753774A2 (en) 2007-02-21

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