WO2006016392A3 - Complex able to detect an analyte, method for its preparation and uses thereof - Google Patents

Complex able to detect an analyte, method for its preparation and uses thereof Download PDF

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Publication number
WO2006016392A3
WO2006016392A3 PCT/IT2005/000488 IT2005000488W WO2006016392A3 WO 2006016392 A3 WO2006016392 A3 WO 2006016392A3 IT 2005000488 W IT2005000488 W IT 2005000488W WO 2006016392 A3 WO2006016392 A3 WO 2006016392A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
complex
detect
preparation
cdcls
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2005/000488
Other languages
French (fr)
Other versions
WO2006016392A2 (en
Inventor
Roberto Burioni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BAIT BIOTECNOLOGIE APPLICATE ITALIANE Srl
BAIT BIOTECNOLOGIE APPLICATE I
Original Assignee
BAIT BIOTECNOLOGIE APPLICATE ITALIANE Srl
BAIT BIOTECNOLOGIE APPLICATE I
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BAIT BIOTECNOLOGIE APPLICATE ITALIANE Srl, BAIT BIOTECNOLOGIE APPLICATE I filed Critical BAIT BIOTECNOLOGIE APPLICATE ITALIANE Srl
Priority to EP05778743A priority Critical patent/EP1776476A2/en
Priority to US11/659,460 priority patent/US20090203548A1/en
Publication of WO2006016392A2 publication Critical patent/WO2006016392A2/en
Publication of WO2006016392A3 publication Critical patent/WO2006016392A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A complex able to detect an analyte (CRA) comprising a particle expressing on its outer surface a compound having specific binding capability (CDCLS) for the analyte and stably including at least one nucleic acid reporter sequence being univocally associated to the CDCLS; process for its construction and uses thereof.
PCT/IT2005/000488 2004-08-10 2005-08-09 Complex able to detect an analyte, method for its preparation and uses thereof Ceased WO2006016392A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP05778743A EP1776476A2 (en) 2004-08-10 2005-08-09 Complex able to detect an analyte, method for its preparation and uses thereof
US11/659,460 US20090203548A1 (en) 2004-08-10 2005-08-09 Complex able to detect an analyte, method for its preparation and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM2004A000406 2004-08-10
IT000406A ITRM20040406A1 (en) 2004-08-10 2004-08-10 COMPLEX ABLE TO DETECT AN ANALITY, PROCEDURE FOR ITS PREPARATION AND USES OF IT.

Publications (2)

Publication Number Publication Date
WO2006016392A2 WO2006016392A2 (en) 2006-02-16
WO2006016392A3 true WO2006016392A3 (en) 2006-04-20

Family

ID=35540677

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2005/000488 Ceased WO2006016392A2 (en) 2004-08-10 2005-08-09 Complex able to detect an analyte, method for its preparation and uses thereof

Country Status (4)

Country Link
US (1) US20090203548A1 (en)
EP (1) EP1776476A2 (en)
IT (1) ITRM20040406A1 (en)
WO (1) WO2006016392A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000029555A1 (en) * 1998-11-17 2000-05-25 Selective Genetics, Inc. Methods using genetic package display for detecting and identifying protein-protein interactions
WO2002039120A1 (en) * 2000-11-09 2002-05-16 Bionova Pharmaceutials, Inc. A method for identifying the proteome of cells using an antibody library microarray
WO2002048403A2 (en) * 2000-12-11 2002-06-20 Hk Pharmaceuticals, Inc. Multiplexed protein expression and activity assay

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9015198D0 (en) * 1990-07-10 1990-08-29 Brien Caroline J O Binding substance

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000029555A1 (en) * 1998-11-17 2000-05-25 Selective Genetics, Inc. Methods using genetic package display for detecting and identifying protein-protein interactions
WO2002039120A1 (en) * 2000-11-09 2002-05-16 Bionova Pharmaceutials, Inc. A method for identifying the proteome of cells using an antibody library microarray
WO2002048403A2 (en) * 2000-12-11 2002-06-20 Hk Pharmaceuticals, Inc. Multiplexed protein expression and activity assay

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BAEK HYUNJUNG ET AL: "An improved helper phage system for efficient isolation of specific antibody molecules in phage display.", NUCLEIC ACIDS RESEARCH. 1 MAR 2002, vol. 30, no. 5, 1 March 2002 (2002-03-01), pages e18, XP002241950, ISSN: 1362-4962 *
BARBAS C F ET AL: "ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES: THE GENE III SITE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 88, September 1991 (1991-09-01), pages 7978 - 7982, XP000652426, ISSN: 0027-8424 *
BURIONI R ET AL: "An improved phage display vector for antibody repertoire cloning by construction of combinatorial libraries", RESEARCH IN VIROLOGY, vol. 148, no. 2, 1997, pages 161 - 164, XP002368463, ISSN: 0923-2516 *
RONDOT SUSANNE ET AL: "A helper phage to improve single-chain antibody presentation in phage display", NATURE BIOTECHNOLOGY, vol. 19, no. 1, January 2001 (2001-01-01), pages 75 - 78, XP002368462, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
US20090203548A1 (en) 2009-08-13
EP1776476A2 (en) 2007-04-25
ITRM20040406A1 (en) 2004-11-10
WO2006016392A2 (en) 2006-02-16

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