WO2006029262A3 - Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment - Google Patents

Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment Download PDF

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Publication number
WO2006029262A3
WO2006029262A3 PCT/US2005/031982 US2005031982W WO2006029262A3 WO 2006029262 A3 WO2006029262 A3 WO 2006029262A3 US 2005031982 W US2005031982 W US 2005031982W WO 2006029262 A3 WO2006029262 A3 WO 2006029262A3
Authority
WO
WIPO (PCT)
Prior art keywords
conditioned medium
angiogenesis
cell
autologous
progenitor cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2005/031982
Other languages
French (fr)
Other versions
WO2006029262A2 (en
Inventor
Ran Kornowski
Shmuel Fuchs
Stephen E Epstein
Martin B Leon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Myocardial Therapeutics Inc
Original Assignee
Myocardial Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Myocardial Therapeutics Inc filed Critical Myocardial Therapeutics Inc
Priority to AU2005282384A priority Critical patent/AU2005282384B2/en
Priority to CA002578815A priority patent/CA2578815A1/en
Priority to EP05796100A priority patent/EP1789538A2/en
Priority to JP2007531326A priority patent/JP2008512198A/en
Publication of WO2006029262A2 publication Critical patent/WO2006029262A2/en
Anticipated expiration legal-status Critical
Publication of WO2006029262A3 publication Critical patent/WO2006029262A3/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A61K38/1709—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/19—Cytokines; Lymphokines; Interferons
    • A61K38/195—Chemokines, e.g. RANTES
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/21—Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

A therapeutic composition is provided that comprises a cell-free conditioned medium containing mixed secretion products of isolated angiogenic progenitor cells obtained from bone marrow, peripheral blood, or adipose tissue. The composition may additionally contain angiogenesis-promoting proteins obtained by transfecting the progenitors cells in culture with an angiogenesis promoting transgene. The composition is useful to promote angiogenesis when introduced into or adjacent to an ischemic site in a patient, such as in myocardium or peripheral limb. Methods are also provided for utilizing such cell-free conditioned medium to deliver angiogenesis-promoting proteins to a patient. The cell-free conditioned medium can also be injected into the blood stream for delivery to the ischemic tissue. The cells can derive from either an autologous or allogenic source and can be lyophilized or frozen for storage.
PCT/US2005/031982 2004-09-08 2005-09-07 Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment Ceased WO2006029262A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2005282384A AU2005282384B2 (en) 2004-09-08 2005-09-07 Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment
CA002578815A CA2578815A1 (en) 2004-09-08 2005-09-07 Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment
EP05796100A EP1789538A2 (en) 2004-09-08 2005-09-07 Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment
JP2007531326A JP2008512198A (en) 2004-09-08 2005-09-07 Conditioned medium of autologous or allogeneic progenitor cells for the treatment of angiogenesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60827204P 2004-09-08 2004-09-08
US60/608,272 2004-09-08

Publications (2)

Publication Number Publication Date
WO2006029262A2 WO2006029262A2 (en) 2006-03-16
WO2006029262A3 true WO2006029262A3 (en) 2009-04-09

Family

ID=36036998

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/031982 Ceased WO2006029262A2 (en) 2004-09-08 2005-09-07 Conditioned medium of autologous or allogenic progenitor cells for angiogenesis treatment

Country Status (6)

Country Link
EP (1) EP1789538A2 (en)
JP (1) JP2008512198A (en)
CN (1) CN101432420A (en)
AU (1) AU2005282384B2 (en)
CA (1) CA2578815A1 (en)
WO (1) WO2006029262A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008024996A2 (en) * 2006-08-24 2008-02-28 Johnstone Brian H Production of neural protective and regenerative factors from stem cells and treatment of nervous system conditions therewith
FI20085839A0 (en) * 2008-09-08 2008-09-08 Timo Ylikomi Methods and tools for soft tissue technology
WO2011127090A1 (en) * 2010-04-05 2011-10-13 Medstar Health Research Institute, Inc. Conditioned medium obtained form stem cells and its use in therapy
US11684574B2 (en) 2016-06-30 2023-06-27 University of Pittsburgh—of the Commonwealth System of Higher Education Artificial cells and delivery devices for use in tissue engineering, and related methods
WO2025208818A1 (en) * 2024-04-02 2025-10-09 深圳近邻生物科技有限公司 Nucleic acid molecule and pharmaceutical composition for treatment of peripheral arterial disease in lower limb, and use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040131601A1 (en) * 2000-03-30 2004-07-08 Foundry Networks, Inc., A Delaward Corporation Injection of bone marrow-derived cells and medium for angiogenesis
US6372494B1 (en) * 1999-05-14 2002-04-16 Advanced Tissue Sciences, Inc. Methods of making conditioned cell culture medium compositions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
REHMAN, J ET AL.: "Peripheral Blood ''Endothelial Progenitor Cells'' Are Derived From Monocyte/Macrophages and Secrete Angiogenic Growth Factors.", CIRCULATION, vol. 107, 2003, pages 1164 - 1169, XP008022340 *
WANG, C. ET AL.: "Mechnical, Cellular, and Molecular Factors Interact to Modulate Circulating Endothelial Cell Progenitors.", AMERICAN JOURNAL OF PHYSIOLOGICAL HEART CIRCULATION PHYSIOLOGY, vol. 286, 2004, pages H1985 - H1993, XP008117720 *

Also Published As

Publication number Publication date
JP2008512198A (en) 2008-04-24
WO2006029262A2 (en) 2006-03-16
AU2005282384B2 (en) 2011-09-01
CA2578815A1 (en) 2006-03-16
AU2005282384A1 (en) 2006-03-16
EP1789538A2 (en) 2007-05-30
CN101432420A (en) 2009-05-13

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