WO2006040313A3 - Mutant expandases - Google Patents

Mutant expandases Download PDF

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Publication number
WO2006040313A3
WO2006040313A3 PCT/EP2005/055148 EP2005055148W WO2006040313A3 WO 2006040313 A3 WO2006040313 A3 WO 2006040313A3 EP 2005055148 W EP2005055148 W EP 2005055148W WO 2006040313 A3 WO2006040313 A3 WO 2006040313A3
Authority
WO
WIPO (PCT)
Prior art keywords
expandase
mutant
activity
mutant expandases
expandases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2005/055148
Other languages
French (fr)
Other versions
WO2006040313A2 (en
Inventor
Dick Schipper
Richard Kerkman
Lourina Madeleine Raamsdonk
Roelof Ary Lans Bovenberg
Stephane J Jenne
Yan Chen
Yong Hong Chen
Xiaodong Gao
Anke Krebber
Claus Krebber
La Ching Charlene To
Matthew Tobin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Priority to CN200580034984.4A priority Critical patent/CN101040043B/en
Priority to EP05801289A priority patent/EP1799817B1/en
Priority to BRPI0515976-8A priority patent/BRPI0515976A/en
Priority to ES05801289T priority patent/ES2395945T3/en
Publication of WO2006040313A2 publication Critical patent/WO2006040313A2/en
Publication of WO2006040313A3 publication Critical patent/WO2006040313A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a mutant expandase that is a variant of a model polypeptide with expandase activity whereby the mutant expandase has an at least 2-fold improved in vitro expandase activity towards adipyl-6-APA in comparison with the model polypeptide with expandase activity.
PCT/EP2005/055148 2004-10-13 2005-10-11 Mutant expandases Ceased WO2006040313A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN200580034984.4A CN101040043B (en) 2004-10-13 2005-10-11 mutant expandase
EP05801289A EP1799817B1 (en) 2004-10-13 2005-10-11 Mutant expandases
BRPI0515976-8A BRPI0515976A (en) 2004-10-13 2005-10-11 mutant expandases
ES05801289T ES2395945T3 (en) 2004-10-13 2005-10-11 Mutant Expandases

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP04105022.0 2004-10-13
EP04105022 2004-10-13
EP05106347.7 2005-07-12
EP05106347 2005-07-12

Publications (2)

Publication Number Publication Date
WO2006040313A2 WO2006040313A2 (en) 2006-04-20
WO2006040313A3 true WO2006040313A3 (en) 2006-10-19

Family

ID=35840672

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/055148 Ceased WO2006040313A2 (en) 2004-10-13 2005-10-11 Mutant expandases

Country Status (6)

Country Link
EP (1) EP1799817B1 (en)
KR (1) KR20070073779A (en)
CN (1) CN101040043B (en)
BR (1) BRPI0515976A (en)
ES (1) ES2395945T3 (en)
WO (1) WO2006040313A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104805047B (en) * 2014-01-23 2017-09-22 中国科学院微生物研究所 Produce recombinant bacterium and its construction method and the application of Cephalosporin G
CN107304418B (en) 2016-04-18 2022-08-26 百瑞全球有限公司 Penicillin expandase mutant, DNA encoding the mutant, kit containing the mutant and use thereof
CN107557348B (en) * 2016-07-01 2022-07-08 百瑞全球有限公司 Cephalosporin hydroxylase mutants, DNA encoding the same, methods of using the same and uses thereof
CN112852913B (en) * 2020-04-17 2021-12-10 中国科学院天津工业生物技术研究所 Deacetyloxycephalosporin C synthase mutants and their application in the synthesis of β-lactam antibiotics
CN113234093A (en) * 2021-04-13 2021-08-10 伊犁川宁生物技术股份有限公司 3-deacetoxy cephalosporin C sodium salt and preparation method thereof
CN115838753B (en) * 2021-09-18 2024-10-29 中国科学院天津工业生物技术研究所 Method for producing 7-amino-deacetyl-cephalosporanic acid
CN117363591B (en) * 2023-10-10 2026-04-10 上海邦林生物科技有限公司 Expandase mutants for the synthesis of G-7-ADCA
CN119286809B (en) * 2024-12-10 2025-10-14 河北凯恩利生物技术有限公司 Penicillin G expandase mutant and polynucleotide, expression vector, and use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020053A2 (en) * 1995-11-27 1997-06-05 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
WO1998002551A2 (en) * 1996-07-16 1998-01-22 Gist-Brocades B.V. Process for the production of adipoyl cephalosporins
WO2001085951A1 (en) * 2000-05-09 2001-11-15 Acs Dobfar Uk Limited A modified expandase and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9407108A (en) * 1993-07-30 1996-08-27 Gist Brocades Nv Process for the preparation and recovery of cephalosporanic 7-amino deacetoxy acid (7-adca) recombinant DNA vector and host cell transformed with a defined vector

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020053A2 (en) * 1995-11-27 1997-06-05 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
WO1998002551A2 (en) * 1996-07-16 1998-01-22 Gist-Brocades B.V. Process for the production of adipoyl cephalosporins
WO2001085951A1 (en) * 2000-05-09 2001-11-15 Acs Dobfar Uk Limited A modified expandase and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE GENESEQ [online] Derwent; 12 March 2002 (2002-03-12), JOHNSON ET AL.: "S. clavuligerus penicillin N expandase 5 residue deletion at C-terminus", XP002370230, Database accession no. AAU11523 *
DATABASE GENESEQ [online] Derwent; 12 March 2002 (2002-03-12), JOHNSON ET AL.: "S. clavuligerus penicillin N expandase K310S deletion mutant", XP002370231, Database accession no. AAU11535 *
DATABASE GENESEQ [online] Derwent; 17 February 1998 (1998-02-17), SUTHERLAND ET AL.: "Expandase mutant C155V", XP002370228, Database accession no. AAW34172 *
DATABASE GENESEQ [online] Derwent; 17 February 1998 (1998-02-17), SUTHERLAND ET AL.: "Expandase mutant R74F", XP002370229, Database accession no. AAW34166 *
DATABASE GENESEQ [online] Derwent; 2 July 1998 (1998-07-02), BOVENBERG ET AL.: "S. clavuligerus expandase protein", XP002370227, Database accession no. AAW40424 *

Also Published As

Publication number Publication date
EP1799817B1 (en) 2012-11-21
WO2006040313A2 (en) 2006-04-20
KR20070073779A (en) 2007-07-10
EP1799817A2 (en) 2007-06-27
ES2395945T3 (en) 2013-02-18
CN101040043A (en) 2007-09-19
BRPI0515976A (en) 2008-08-12
CN101040043B (en) 2014-05-28

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