WO2006045936A3 - Systeme et procede d’imagerie microscopique multiphotonique fibre d’un echantillon - Google Patents

Systeme et procede d’imagerie microscopique multiphotonique fibre d’un echantillon Download PDF

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Publication number
WO2006045936A3
WO2006045936A3 PCT/FR2005/002630 FR2005002630W WO2006045936A3 WO 2006045936 A3 WO2006045936 A3 WO 2006045936A3 FR 2005002630 W FR2005002630 W FR 2005002630W WO 2006045936 A3 WO2006045936 A3 WO 2006045936A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
image guide
fibered
carrying
multiphoton
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2005/002630
Other languages
English (en)
Other versions
WO2006045936A2 (fr
Inventor
Bertrand Viellerobe
Francois Lacombe
Alexandre Loiseau
Frederic Louradour
Mickael Lelek
Alain Barthelemy
Dominique Pagnoux
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Limoges
Mauna Kea Technologies SA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Limoges
Mauna Kea Technologies SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Limoges, Mauna Kea Technologies SA filed Critical Centre National de la Recherche Scientifique CNRS
Priority to EP05812037A priority Critical patent/EP1817620B8/fr
Priority to ES05812037T priority patent/ES2396426T3/es
Priority to JP2007537342A priority patent/JP5102622B2/ja
Priority to US11/665,768 priority patent/US8237131B2/en
Priority to AU2005298494A priority patent/AU2005298494B2/en
Priority to CA2584748A priority patent/CA2584748C/fr
Publication of WO2006045936A2 publication Critical patent/WO2006045936A2/fr
Publication of WO2006045936A3 publication Critical patent/WO2006045936A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2476—Non-optical details, e.g. housings, mountings, supports
    • G02B23/2484—Arrangements in relation to a camera or imaging device
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
    • A61B5/0086—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters using infrared radiation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B23/00—Telescopes, e.g. binoculars; Periscopes; Instruments for viewing the inside of hollow bodies; Viewfinders; Optical aiming or sighting devices
    • G02B23/24—Instruments or systems for viewing the inside of hollow bodies, e.g. fibrescopes
    • G02B23/2407—Optical details
    • G02B23/2461—Illumination
    • G02B23/2469—Illumination using optical fibres
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0062—Arrangements for scanning
    • A61B5/0068—Confocal scanning
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/08—Optical fibres; light guides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/105—Purely optical scan
    • G01N2201/1053—System of scan mirrors for composite motion of beam
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
    • G02B6/04—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings formed by bundles of fibres

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Surgery (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Astronomy & Astrophysics (AREA)
  • Radiology & Medical Imaging (AREA)
  • Multimedia (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)
  • Endoscopes (AREA)

Abstract

L'invention concerne un système d'imagerie multiphotonique fibre d'un échantillon (10) pour une utilisation en endoscopie ou en microscopie de fluorescence, Ce système comprend : un laser puisé femtoseconde (1, 2) pour générer un rayonnement laser multiphotonique d'excitation, un guide d'image (8) constitué d'une pluralité de fibres optiques et permettant d'illuminer l'échantillon par un balayage point par point dans un plan subsurfacique, des moyens de pré-compensation (4) pour compenser des effets de dispersion des impulsions d'excitation dans le guide d'image (8), ces moyens étant disposés entre le laser puisé et le guide d'image (8), des moyens de balayage pour diriger tour à tour le faisceau laser d'excitation dans une fibre du guide d'image, et notamment une tête optique (9) pour focaliser le faisceau laser d'excitation sortant du guide d'image dans l'échantillon (10).
PCT/FR2005/002630 2004-10-22 2005-10-21 Systeme et procede d’imagerie microscopique multiphotonique fibre d’un echantillon Ceased WO2006045936A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP05812037A EP1817620B8 (fr) 2004-10-22 2005-10-21 Systeme et procede d`imagerie microscopique multiphotonique fibre d`un echantillon
ES05812037T ES2396426T3 (es) 2004-10-22 2005-10-21 Sistema y procedimiento de diagnóstico por imagen microscópica multifotónica fibrada de una muestra
JP2007537342A JP5102622B2 (ja) 2004-10-22 2005-10-21 検体のファイバ式マルチフォトン式顕微鏡イメージング装置および方法
US11/665,768 US8237131B2 (en) 2004-10-22 2005-10-21 System and method for carrying out fibre-type multiphoton microscopic imaging of a sample
AU2005298494A AU2005298494B2 (en) 2004-10-22 2005-10-21 System and method for carrying out fibered multiphoton microscopic imagery of a sample
CA2584748A CA2584748C (fr) 2004-10-22 2005-10-21 Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0411313 2004-10-22
FR0411313A FR2877103B1 (fr) 2004-10-22 2004-10-22 Systeme et procede d'imagerie microscopique multiphotonique fibre d'un echantillon

Publications (2)

Publication Number Publication Date
WO2006045936A2 WO2006045936A2 (fr) 2006-05-04
WO2006045936A3 true WO2006045936A3 (fr) 2006-06-22

Family

ID=34951919

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2005/002630 Ceased WO2006045936A2 (fr) 2004-10-22 2005-10-21 Systeme et procede d’imagerie microscopique multiphotonique fibre d’un echantillon

Country Status (8)

Country Link
US (1) US8237131B2 (fr)
EP (1) EP1817620B8 (fr)
JP (1) JP5102622B2 (fr)
AU (1) AU2005298494B2 (fr)
CA (1) CA2584748C (fr)
ES (1) ES2396426T3 (fr)
FR (1) FR2877103B1 (fr)
WO (1) WO2006045936A2 (fr)

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US8059274B2 (en) * 2007-12-07 2011-11-15 The Spectranetics Corporation Low-loss polarized light diversion
DE102009029831A1 (de) 2009-06-17 2011-01-13 W.O.M. World Of Medicine Ag Vorrichtung und Verfahren für die Mehr-Photonen-Fluoreszenzmikroskopie zur Gewinnung von Informationen aus biologischem Gewebe
US8507877B2 (en) * 2009-11-25 2013-08-13 Ofs Fitel, Llc Non-linear optical system and techniques
US9304308B2 (en) * 2009-12-09 2016-04-05 Advanced Micro Devices, Inc. Laser scanning module including an optical isolator
WO2011079126A2 (fr) * 2009-12-23 2011-06-30 Laser Biopsy, Inc. Procédé et appareil pour système d'imagerie microscopique à large champ de vue et à rendement de collection élevé
EP2542145B1 (fr) 2010-03-05 2020-08-12 The General Hospital Corporation Systèmes qui procurent des images microscopiques d'au moins une structure anatomique à une résolution particulière
US8792098B2 (en) 2011-06-01 2014-07-29 Digital Light Innovations System and method for hyperspectral illumination
US8891087B2 (en) 2011-06-01 2014-11-18 Digital Light Innovations System and method for hyperspectral imaging
US9356413B2 (en) * 2011-09-30 2016-05-31 Olympus Corporation Laser source apparatus and laser microscope
EP2785250A4 (fr) * 2011-11-28 2015-07-29 Univ Leland Stanford Junior Système et procédé utiles pour imagerie de sarcomère par l'intermédiaire de microscopie basée sur objectif
WO2015108991A2 (fr) 2014-01-17 2015-07-23 Imra America, Inc. Modification de materiaux transparents induite par traitement laser
JP2017513211A (ja) * 2014-02-28 2017-05-25 イムラ アメリカ インコーポレイテッド 顕微鏡に適用される多波長超短パルスの生成及び放出
DE112015001154T5 (de) * 2014-04-24 2016-12-08 Olympus Corporation Mikroskop und Mikroskopie-Verfahren
JP2015227778A (ja) * 2014-05-30 2015-12-17 ソニー株式会社 画像取得装置及び画像取得方法
FR3030956B1 (fr) * 2014-12-18 2018-03-23 Centre National De La Recherche Scientifique - Cnrs Dispositif de transport et de controle d'impulsions lumineuses pour l'imagerie endo-microscopique sans lentille
JP6594437B2 (ja) 2015-09-15 2019-10-23 オリンパス株式会社 顕微鏡および顕微鏡観察方法
CN115919266A (zh) 2016-03-08 2023-04-07 恩斯派克特拉健康公司 皮肤疾病的非侵入式检测
WO2017174998A1 (fr) 2016-04-06 2017-10-12 The University Court Of The University Of Edinburgh Appareil et procédé d'imagerie endoscopique
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WO2020010330A1 (fr) * 2018-07-06 2020-01-09 The Trustees Of Columbia University In The City Of New York Modification de tissu de collagène
CN113473900B (zh) 2018-11-13 2024-09-24 恩斯派克特拉健康公司 用于生成深度剖面的方法和系统
CN109656014B (zh) * 2019-01-31 2024-03-19 北京超维景生物科技有限公司 多路荧光收集装置及三维非线性激光扫描腔体内窥镜
CN113349708B (zh) * 2021-06-08 2023-05-26 之江实验室 一种基于大色散镜头的双模态高分辨大深度显微内窥成像系统
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Also Published As

Publication number Publication date
FR2877103B1 (fr) 2012-02-10
JP2008517291A (ja) 2008-05-22
JP5102622B2 (ja) 2012-12-19
AU2005298494B2 (en) 2011-08-11
EP1817620B8 (fr) 2012-12-12
US8237131B2 (en) 2012-08-07
AU2005298494A1 (en) 2006-05-04
EP1817620A2 (fr) 2007-08-15
US20070290145A1 (en) 2007-12-20
CA2584748A1 (fr) 2006-05-04
FR2877103A1 (fr) 2006-04-28
CA2584748C (fr) 2015-10-13
EP1817620B1 (fr) 2012-09-26
ES2396426T3 (es) 2013-02-21
WO2006045936A2 (fr) 2006-05-04

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