WO2006082402A3 - Biochemical reagents and their uses - Google Patents

Biochemical reagents and their uses Download PDF

Info

Publication number
WO2006082402A3
WO2006082402A3 PCT/GB2006/000350 GB2006000350W WO2006082402A3 WO 2006082402 A3 WO2006082402 A3 WO 2006082402A3 GB 2006000350 W GB2006000350 W GB 2006000350W WO 2006082402 A3 WO2006082402 A3 WO 2006082402A3
Authority
WO
WIPO (PCT)
Prior art keywords
region
oligonucleotide
nucleic acid
reaction mixture
double stranded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2006/000350
Other languages
French (fr)
Other versions
WO2006082402A2 (en
Inventor
David James Squirrell
Martin Alan Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enigma Diagnostics Ltd
Original Assignee
Enigma Diagnostics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enigma Diagnostics Ltd filed Critical Enigma Diagnostics Ltd
Priority to EP06709601A priority Critical patent/EP1844160A2/en
Priority to US11/814,849 priority patent/US20080131891A1/en
Priority to JP2007552731A priority patent/JP2008528021A/en
Publication of WO2006082402A2 publication Critical patent/WO2006082402A2/en
Publication of WO2006082402A3 publication Critical patent/WO2006082402A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for adding a first and a second functional nucleic acid sequence to a reaction mixture, in particular an amplification reaction mixture in a predetermined stoichiometry and/or at a predetermined point in time, said method comprising adding to the reaction mixture an oligonucleotide comprising a first and a second functional nucleic acid sequence separated by a spacer sequence, said spacer sequence comprising a region which, when double stranded, comprises a cleavable region, forming a cleavable double stranded region within the spacer region of said oligonucleotide, and cleaving the double stranded region within said oligonucleotide. Oligonucleotides for use in the method, and comprising a first and a second functional nucleic acid sequence, such as primers or probes used in an amplification reaction, separated by a spacer sequence, is also provided.
PCT/GB2006/000350 2005-02-01 2006-02-01 Biochemical reagents and their uses Ceased WO2006082402A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP06709601A EP1844160A2 (en) 2005-02-01 2006-02-01 Biochemical reagents and their uses
US11/814,849 US20080131891A1 (en) 2005-02-01 2006-02-01 Biochemical Reagents And Their Uses
JP2007552731A JP2008528021A (en) 2005-02-01 2006-02-01 Biochemical reagents and their use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0502010.2A GB0502010D0 (en) 2005-02-01 2005-02-01 Biochemical reagents and their uses
GB0502010.2 2005-02-01

Publications (2)

Publication Number Publication Date
WO2006082402A2 WO2006082402A2 (en) 2006-08-10
WO2006082402A3 true WO2006082402A3 (en) 2006-10-19

Family

ID=34307760

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/000350 Ceased WO2006082402A2 (en) 2005-02-01 2006-02-01 Biochemical reagents and their uses

Country Status (5)

Country Link
US (1) US20080131891A1 (en)
EP (1) EP1844160A2 (en)
JP (1) JP2008528021A (en)
GB (1) GB0502010D0 (en)
WO (1) WO2006082402A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201004339D0 (en) * 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002024955A2 (en) * 2000-09-19 2002-03-28 Atom Sciences, Inc. Detection of unlabeled hybridized dna and rna using restriction enzyme digestion
WO2002079520A1 (en) * 2001-03-30 2002-10-10 Applied Gene Technologies, Inc. Methods and compositions for analyzing nucleotide sequence mismatches using rnase h
WO2002088387A2 (en) * 2001-04-30 2002-11-07 The Secretary Of State For Defence Amplification process
EP1319719A1 (en) * 2001-12-03 2003-06-18 Roche Diagnostics GmbH Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro
WO2004044549A2 (en) * 2002-11-06 2004-05-27 Geneohm Sciences Universal tag assay

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5853990A (en) * 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
JPH1156398A (en) * 1997-08-11 1999-03-02 Hamamatsu Photonics Kk Assay of double-stranded nucleic acid splitting enzyme activity
JP4242637B2 (en) * 2002-12-12 2009-03-25 オリンパス株式会社 Splicing variant detection method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002024955A2 (en) * 2000-09-19 2002-03-28 Atom Sciences, Inc. Detection of unlabeled hybridized dna and rna using restriction enzyme digestion
WO2002079520A1 (en) * 2001-03-30 2002-10-10 Applied Gene Technologies, Inc. Methods and compositions for analyzing nucleotide sequence mismatches using rnase h
WO2002088387A2 (en) * 2001-04-30 2002-11-07 The Secretary Of State For Defence Amplification process
EP1319719A1 (en) * 2001-12-03 2003-06-18 Roche Diagnostics GmbH Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro
WO2004044549A2 (en) * 2002-11-06 2004-05-27 Geneohm Sciences Universal tag assay

Also Published As

Publication number Publication date
EP1844160A2 (en) 2007-10-17
WO2006082402A2 (en) 2006-08-10
JP2008528021A (en) 2008-07-31
US20080131891A1 (en) 2008-06-05
GB0502010D0 (en) 2005-03-09

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