WO2006095342A3 - Natural tissue-derived decellularized matrix and methods of generating and using same - Google Patents

Natural tissue-derived decellularized matrix and methods of generating and using same Download PDF

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Publication number
WO2006095342A3
WO2006095342A3 PCT/IL2006/000304 IL2006000304W WO2006095342A3 WO 2006095342 A3 WO2006095342 A3 WO 2006095342A3 IL 2006000304 W IL2006000304 W IL 2006000304W WO 2006095342 A3 WO2006095342 A3 WO 2006095342A3
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WO
WIPO (PCT)
Prior art keywords
tissue
generating
methods
decellularized
same
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2006/000304
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French (fr)
Other versions
WO2006095342A2 (en
Inventor
Marcelle Machluf
Yuval Eitan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technion Research and Development Foundation Ltd
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Technion Research and Development Foundation Ltd
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Filing date
Publication date
Application filed by Technion Research and Development Foundation Ltd filed Critical Technion Research and Development Foundation Ltd
Priority to US11/885,640 priority Critical patent/US9216236B2/en
Priority to EP06711286.2A priority patent/EP1856246B1/en
Publication of WO2006095342A2 publication Critical patent/WO2006095342A2/en
Publication of WO2006095342A3 publication Critical patent/WO2006095342A3/en
Anticipated expiration legal-status Critical
Priority to IL185837A priority patent/IL185837A0/en
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3873—Muscle tissue, e.g. sphincter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3633—Extracellular matrix [ECM]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/367—Muscle tissue, e.g. sphincter
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3826—Muscle cells, e.g. smooth muscle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/20—Materials or treatment for tissue regeneration for reconstruction of the heart, e.g. heart valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Zoology (AREA)
  • Dermatology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Vascular Medicine (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cardiology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Decellularized tissue-derived extracellular matrices (ECM) and methods of generating and using same are provided. The method of generating a decellularized matrix includes the steps of: (a) subjecting the tissue to washes and a hypertonic buffer; (b) subjecting the tissue to an enzymatic proteolytic digestion with an enzyme such as trypsin; and (c) removing all cellular components from the tissue using a detergent solution which includes Triton-X-100 and ammonium hydroxide. Specifically, there is provided a decellularized myocardium-derived matrix which is completely devoid of all cellular components and hence non-immunogenic in a subject, exhibits suitable structural and mechanical properties for cardiac tissue engineering or replacement therapy of damaged cardiac tissue, and is capable of remodeling upon seeding of cells.
PCT/IL2006/000304 2005-03-07 2006-03-07 Natural tissue-derived decellularized matrix and methods of generating and using same Ceased WO2006095342A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US11/885,640 US9216236B2 (en) 2005-03-07 2006-03-07 Natural tissue-derived decellularized matrix and methods of generating and using same
EP06711286.2A EP1856246B1 (en) 2005-03-07 2006-03-07 Natural tissue-derived decellularized matrix and methods of generating and using same
IL185837A IL185837A0 (en) 2005-03-07 2007-09-09 Natural tissue-derived decellularized matrix and methods of generating and using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65858505P 2005-03-07 2005-03-07
US60/658,585 2005-03-07

Publications (2)

Publication Number Publication Date
WO2006095342A2 WO2006095342A2 (en) 2006-09-14
WO2006095342A3 true WO2006095342A3 (en) 2007-03-01

Family

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Family Applications (1)

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PCT/IL2006/000304 Ceased WO2006095342A2 (en) 2005-03-07 2006-03-07 Natural tissue-derived decellularized matrix and methods of generating and using same

Country Status (3)

Country Link
US (1) US9216236B2 (en)
EP (1) EP1856246B1 (en)
WO (1) WO2006095342A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8109995B2 (en) 2002-01-04 2012-02-07 Colibri Heart Valve Llc Percutaneously implantable replacement heart valve device and method of making same
US8361144B2 (en) 2010-03-01 2013-01-29 Colibri Heart Valve Llc Percutaneously deliverable heart valve and methods associated therewith
US9119738B2 (en) 2010-06-28 2015-09-01 Colibri Heart Valve Llc Method and apparatus for the endoluminal delivery of intravascular devices
US9789224B2 (en) 2010-08-24 2017-10-17 The Regents Of The University Of California Compositions and methods for cardiac therapy

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1856246B1 (en) 2005-03-07 2015-07-15 Technion Research & Development Foundation Limited Natural tissue-derived decellularized matrix and methods of generating and using same
KR20230146124A (en) 2006-10-06 2023-10-18 셀룰래리티 인코포레이티드 Native(telopeptide) Placental Collagen Compositions
US8361503B2 (en) 2007-03-02 2013-01-29 University of Pittsburgh—of the Commonwealth System of Higher Education Extracellular matrix-derived gels and related methods
CN101274106B (en) * 2008-03-24 2011-11-09 中山大学中山眼科中心 Method for preparing acellular matrix
CN103751842A (en) * 2008-07-30 2014-04-30 米辛瑟斯有限公司 Tissue scaffolds derived from forestomach extracellular matrix
ES2628100T3 (en) 2008-09-30 2017-08-01 The Regents Of The University Of California Compositions comprising decellularized extracellular matrix obtained from cardiac tissue
US9150318B1 (en) * 2009-01-02 2015-10-06 Lifecell Corporation Method for sterilizing an acellular tissue matrix
RU2407459C1 (en) * 2009-07-27 2010-12-27 Учреждение Российской академии медицинских наук Научно-исследовательский институт кардиологии Сибирского отделения РАМН Method of obtaining connective tissue frame of main vessel of mammals and humans
WO2011038023A1 (en) * 2009-09-22 2011-03-31 Colorado State University Research Foundation Methods for processing biological tissues
US20110165676A1 (en) * 2009-11-06 2011-07-07 The Children's Mercy Hospital Method for decellularization
MX2012006759A (en) 2009-12-17 2012-07-30 Univ Kingston Decellularized adipose tissue.
CN103391756B (en) 2010-12-14 2016-06-01 科利柏心脏瓣膜有限责任公司 Including have integrate leaflet folded membrane cusp can percutaneous conveying cardiac valve
WO2012142569A2 (en) * 2011-04-15 2012-10-18 The Regents Of The University Of California Decellularized extracellular matrix
US9238793B2 (en) 2011-04-28 2016-01-19 Lifecell Corporation Method for enzymatic treatment of tissue products
US10207025B2 (en) 2011-04-28 2019-02-19 Lifecell Corporation Method for enzymatic treatment of tissue products
CN105854083A (en) 2011-04-28 2016-08-17 生命细胞公司 Method for enzymatic treatment of tissue products
WO2013028968A1 (en) * 2011-08-25 2013-02-28 Wisconsin Alumni Research Foundation 3-dimensional cardiac fibroblast derived extracellular matrix
WO2013036708A2 (en) 2011-09-07 2013-03-14 The Regents Of The University Of California Compositions and methods for tissue repair with extracellular matrices
EP3777906A1 (en) * 2012-07-06 2021-02-17 LifeCell Corporation Decellularized muscle matrix
US10085946B2 (en) 2012-09-04 2018-10-02 Technion Research & Development Foundation Limited Use of decellularized extracellular matrix for encapsulating cells
JP6650393B2 (en) 2013-06-13 2020-02-19 オージェネシス リミテッド Cell population, transdifferentiation method and use thereof
US20150037436A1 (en) 2013-07-30 2015-02-05 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
WO2015066668A1 (en) * 2013-11-04 2015-05-07 Lifecell Corporation Methods of removing alpha-galactose
WO2015081226A1 (en) 2013-11-27 2015-06-04 The Johns Hopkins University Engineered cardiac derived compositions and methods of use
AU2015231110B2 (en) 2014-03-21 2019-03-07 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Methods for preparation of a terminally sterilized hydrogel derived from extracellular matrix
MA41296A (en) 2014-12-30 2017-11-07 Orgenesis Ltd TRANSDIFFERENTIATION PROCESSES AND METHODS FOR USING THE SAME
US10898609B2 (en) * 2015-07-15 2021-01-26 University Of Florida Research Foundation, Inc. Tissue decellularization methods
US10912864B2 (en) 2015-07-24 2021-02-09 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
US11185610B2 (en) * 2015-08-11 2021-11-30 Acro Biomedical Company. Ltd. Preparation of acellular cartilage graft and uses thereof
US11052175B2 (en) 2015-08-19 2021-07-06 Musculoskeletal Transplant Foundation Cartilage-derived implants and methods of making and using same
WO2017044570A1 (en) * 2015-09-08 2017-03-16 Clemson University Decellularized biomaterial and method for formation
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CA3049990A1 (en) 2017-03-02 2018-09-07 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Extracellular matrix (ecm) hydrogel and soluble fraction thereof for the treatment of cancer
EP3635106A4 (en) 2017-05-08 2021-01-06 Orgenesis Ltd. TRANSDIFFERENTIAL CELL POPULATIONS AND METHOD OF USING THEM
EP3624864A1 (en) * 2017-05-19 2020-03-25 Colorado State University Research Foundation Methods for preparing a decellularized muscle scaffold
US10217720B2 (en) 2017-06-15 2019-02-26 Invensas Corporation Multi-chip modules formed using wafer-level processing of a reconstitute wafer
WO2019051476A1 (en) 2017-09-11 2019-03-14 Incubar, LLC Conduit vascular implant sealing device for reducing endoleak
KR102042114B1 (en) * 2017-12-29 2019-11-07 울산과학기술원 Manufacturing method for decellularized tissue and decellularized tissue manufactured by the same
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CN110732041A (en) * 2019-10-12 2020-01-31 广东省人民医院(广东省医学科学院) decellularized small-caliber intravascular stent and preparation method thereof
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WO2025059306A1 (en) * 2023-09-14 2025-03-20 Pioneer Hi-Bred International, Inc. Insect primary cell isolation methods
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996032905A1 (en) * 1995-04-19 1996-10-24 St. Jude Medical, Inc. Matrix substrate for a viable body tissue-derived prosthesis and method for making the same
WO2002014480A2 (en) * 2000-08-16 2002-02-21 Duke University Decellularized tissue engineered constructs and tissues
WO2005009497A1 (en) * 2003-07-17 2005-02-03 Boston Scientific Limited Decellularized extracellular matrix of conditioned body tissues and uses thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6734018B2 (en) 1999-06-07 2004-05-11 Lifenet Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced
US6743574B1 (en) 2000-09-12 2004-06-01 Lifenet Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced
US6866686B2 (en) 2000-01-28 2005-03-15 Cryolife, Inc. Tissue graft
AU2001288458A1 (en) 2000-08-28 2002-03-13 Collagenesis, Inc. Methods for processing animal tissues
US6933103B1 (en) 2001-03-21 2005-08-23 Brennen Medical, Inc. Biocompatible tissue for therapeutic use and method of making same
JP4426285B2 (en) 2001-06-28 2010-03-03 クック・バイオテック・インコーポレーテッド Graft prosthesis device containing renal capsule collagen
EP1517715A2 (en) 2002-06-28 2005-03-30 Cardio, Inc. Decellularized tissue
EP1856246B1 (en) 2005-03-07 2015-07-15 Technion Research & Development Foundation Limited Natural tissue-derived decellularized matrix and methods of generating and using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996032905A1 (en) * 1995-04-19 1996-10-24 St. Jude Medical, Inc. Matrix substrate for a viable body tissue-derived prosthesis and method for making the same
WO2002014480A2 (en) * 2000-08-16 2002-02-21 Duke University Decellularized tissue engineered constructs and tissues
WO2005009497A1 (en) * 2003-07-17 2005-02-03 Boston Scientific Limited Decellularized extracellular matrix of conditioned body tissues and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BADYLAK STEPHEN ET AL: "Extracellular matrix for myocardial repair.", THE HEART SURGERY FORUM. 2003, vol. 6, no. 2, 2003, pages E20 - E26, XP002405778, ISSN: 1522-6662 *
GRAUSS R W ET AL: "Histological evaluation of decellularised porcine aortic valves: matrix changes due to different decellularisation methods", EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, SPRINGER VERLAG, BERLIN, DE, vol. 27, no. 4, April 2005 (2005-04-01), pages 566 - 571, XP004794090, ISSN: 1010-7940 *
KIM WON GON ET AL: "Time related histopathologic changes of acellularized xenogenic pulmonary valved conduits.", ASAIO JOURNAL (AMERICAN SOCIETY FOR ARTIFICIAL INTERNAL ORGANS : 1992) 2004 NOV-DEC, vol. 50, no. 6, November 2004 (2004-11-01), pages 601 - 605, XP009074492, ISSN: 1058-2916 *
WILSON G J ET AL: "Acellular matrix allograft small caliber vascular prostheses.", ASAIO TRANSACTIONS / AMERICAN SOCIETY FOR ARTIFICIAL INTERNAL ORGANS. 1990 JUL-SEP, vol. 36, no. 3, July 1990 (1990-07-01), pages M340 - M343, XP009074482, ISSN: 0889-7190 *
ZELTINGER JOAN ET AL: "Development and characterization of tissue-engineered aortic valves", TISSUE ENGINEERING, vol. 7, no. 1, February 2001 (2001-02-01), pages 9 - 22, XP002405759, ISSN: 1076-3279 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8109995B2 (en) 2002-01-04 2012-02-07 Colibri Heart Valve Llc Percutaneously implantable replacement heart valve device and method of making same
US8308797B2 (en) 2002-01-04 2012-11-13 Colibri Heart Valve, LLC Percutaneously implantable replacement heart valve device and method of making same
US8790398B2 (en) 2002-01-04 2014-07-29 Colibri Heart Valve Llc Percutaneously implantable replacement heart valve device and method of making same
US8900294B2 (en) 2002-01-04 2014-12-02 Colibri Heart Valve Llc Method of controlled release of a percutaneous replacement heart valve
US9125739B2 (en) 2002-01-04 2015-09-08 Colibri Heart Valve Llc Percutaneous replacement heart valve and a delivery and implantation system
US9186248B2 (en) 2002-01-04 2015-11-17 Colibri Heart Valve Llc Percutaneously implantable replacement heart valve device and method of making same
US9554898B2 (en) 2002-01-04 2017-01-31 Colibri Heart Valve Llc Percutaneous prosthetic heart valve
US8361144B2 (en) 2010-03-01 2013-01-29 Colibri Heart Valve Llc Percutaneously deliverable heart valve and methods associated therewith
US9119738B2 (en) 2010-06-28 2015-09-01 Colibri Heart Valve Llc Method and apparatus for the endoluminal delivery of intravascular devices
US9789224B2 (en) 2010-08-24 2017-10-17 The Regents Of The University Of California Compositions and methods for cardiac therapy
US10456501B2 (en) 2010-08-24 2019-10-29 Ventrix, Inc. Compositions and methods for cardiac therapy

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