WO2006127809A3 - Methods for expansion and analysis of cultured hematopoietic stem cells - Google Patents

Methods for expansion and analysis of cultured hematopoietic stem cells Download PDF

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Publication number
WO2006127809A3
WO2006127809A3 PCT/US2006/020078 US2006020078W WO2006127809A3 WO 2006127809 A3 WO2006127809 A3 WO 2006127809A3 US 2006020078 W US2006020078 W US 2006020078W WO 2006127809 A3 WO2006127809 A3 WO 2006127809A3
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Prior art keywords
angiopoietin
stem cells
hematopoietic stem
methods
protein
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Ceased
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PCT/US2006/020078
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French (fr)
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WO2006127809A2 (en
Inventor
Chengcheng Zhang
Harvey Lodish
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Whitehead Institute for Biomedical Research
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Whitehead Institute for Biomedical Research
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Priority to JP2008513668A priority Critical patent/JP5139271B2/en
Priority to EP06771063A priority patent/EP1899457B1/en
Publication of WO2006127809A2 publication Critical patent/WO2006127809A2/en
Publication of WO2006127809A3 publication Critical patent/WO2006127809A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/17—Angiopoietin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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  • Microbiology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Developmental Biology & Embryology (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • AIDS & HIV (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Methods and kits for propagating hematopoietic stem cells are provided. The methods comprise culturing cells in medium comprising one or more angiopoietin-like proteins, under conditions sufficient for expansion of HSCs. Angiopoietin-like proteins include angiopoietin-like protein 2, angiopoietin-like protein 3, angiopoietin-like protein 4, angiopoietin-like protein 5, angiopoietin-like protein 7, and microfibrillar-associated glycoprotein (Mfap4). Methods for identifying hematopoietic stem cells are provided and isolated hematopoietic stem cells are also provided.
PCT/US2006/020078 2005-05-24 2006-05-23 Methods for expansion and analysis of cultured hematopoietic stem cells Ceased WO2006127809A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008513668A JP5139271B2 (en) 2005-05-24 2006-05-23 Method for expanding and analyzing cultured hematopoietic stem cells
EP06771063A EP1899457B1 (en) 2005-05-24 2006-05-23 Methods for expansion and analysis of cultured hematopoietic stem cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US68414705P 2005-05-24 2005-05-24
US60/684,147 2005-05-24
US75321205P 2005-12-22 2005-12-22
US60/753,212 2005-12-22

Publications (2)

Publication Number Publication Date
WO2006127809A2 WO2006127809A2 (en) 2006-11-30
WO2006127809A3 true WO2006127809A3 (en) 2007-02-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/020078 Ceased WO2006127809A2 (en) 2005-05-24 2006-05-23 Methods for expansion and analysis of cultured hematopoietic stem cells

Country Status (4)

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US (3) US7807464B2 (en)
EP (1) EP1899457B1 (en)
JP (1) JP5139271B2 (en)
WO (1) WO2006127809A2 (en)

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WO2004069172A2 (en) 2003-01-30 2004-08-19 The Government of the United States of America as represented by the Department of Veterans Affairs Multilineage-inducible cells and uses thereof
CN100377165C (en) * 2003-04-24 2008-03-26 皇家飞利浦电子股份有限公司 Noninvasive left ventricular volume measurement
JP5139271B2 (en) 2005-05-24 2013-02-06 ホワイトヘッド インスティチュート フォー バイオメディカル リサーチ Method for expanding and analyzing cultured hematopoietic stem cells
WO2008024996A2 (en) 2006-08-24 2008-02-28 Johnstone Brian H Production of neural protective and regenerative factors from stem cells and treatment of nervous system conditions therewith
EP2094839B1 (en) * 2006-12-08 2020-02-05 University of Rochester Expansion of hematopoietic stem cells
US8609411B2 (en) 2007-05-04 2013-12-17 Whitehead Institute For Biomedical Research Ex vivo expansion of human hematopoietic stem cells
CN102171337A (en) * 2008-08-26 2011-08-31 智能纳米股份有限公司 Enhanced animal cell growth using ultrasound
US8962290B2 (en) 2008-08-26 2015-02-24 Intelligentnano Inc. Enhanced animal cell growth using ultrasound
US9012192B2 (en) 2008-08-26 2015-04-21 Intelligentnano Inc. Ultrasound enhanced growth of microorganisms
WO2010062999A1 (en) * 2008-11-28 2010-06-03 Stematix, Inc Diabetes cell therapy
EP2419511B1 (en) * 2009-04-09 2018-01-17 Sangamo Therapeutics, Inc. Targeted integration into stem cells
WO2010138873A1 (en) * 2009-05-29 2010-12-02 Maroun Khoury Long term expansion of human hematopoietic stem cells
JP5602850B2 (en) 2009-07-14 2014-10-08 ザ スクリプス リサーチ インスティテュート Mesenchymal stem cell differentiation
KR101334208B1 (en) 2011-09-01 2013-11-29 서울대학교산학협력단 A Method for Inducing Myogenic-Lineage Differentiation of Stem Cells
US20150174203A1 (en) * 2012-05-30 2015-06-25 Icahn School Of Medicine At Mount Sinai Compositions And Methods For Modulating Pro-Inflammatory Immune Response
UY35368A (en) 2013-03-08 2014-10-31 Irm Llc PEPTIDES AND COMPOSITIONS FOR THE TREATMENT OF ARTICULAR DAMAGE
MA38369B1 (en) 2013-03-08 2018-10-31 Novartis Ag Peptides and compositions for the treatment of a lesion of the joint
WO2014183066A2 (en) 2013-05-10 2014-11-13 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
CN103509101B (en) * 2013-08-12 2016-11-02 中国科学院广州生物医药与健康研究院 A kind of cytokine and its medium for expanding umbilical cord blood hematopoietic stem cells
CA2948871A1 (en) * 2014-05-22 2015-11-26 Fred Hutchinson Cancer Research Center Lilrb2 and notch-mediated expansion of hematopoietic precursor cells
WO2016014794A1 (en) 2014-07-25 2016-01-28 Sangamo Biosciences, Inc. Methods and compositions for modulating nuclease-mediated genome engineering in hematopoietic stem cells
DK3194570T3 (en) 2014-09-16 2021-09-13 Sangamo Therapeutics Inc PROCEDURES AND COMPOSITIONS FOR NUCLEASE MEDIATED GENOMIFICATION AND CORRECTION IN HEMATOPOETIC STEM CELLS
US12146165B2 (en) 2018-03-23 2024-11-19 The Children's Medical Center Corporation Endothelial cell factors and methods thereof
TW202027794A (en) 2018-10-03 2020-08-01 瑞士商諾華公司 Sustained delivery of angiopoetin-like 3 polypeptides
WO2021013669A1 (en) * 2019-07-24 2021-01-28 Miltenyi Biotec B.V. & Co. KG Assay for identifing colony-forming cells
WO2023196932A2 (en) * 2022-04-06 2023-10-12 The Regents Of The University Of California Isolating active t-cell cars from bulk transduced human t-cells

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WO2000052167A1 (en) * 1999-03-02 2000-09-08 Regeneron Pharmaceuticals, Inc. Angiopoietin-related protein and nucleic acids
EP1308511A1 (en) * 2001-10-31 2003-05-07 Mermaid Pharmaceuticals GmbH Use of angiopoietin-like 1 and angiopoietin-like 2 nucleic acids and proteins for the treatment of blood-related disorders and defects in vasculature.

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Also Published As

Publication number Publication date
US20070020757A1 (en) 2007-01-25
WO2006127809A2 (en) 2006-11-30
US20140017784A1 (en) 2014-01-16
EP1899457B1 (en) 2012-09-05
JP5139271B2 (en) 2013-02-06
US7807464B2 (en) 2010-10-05
EP1899457A2 (en) 2008-03-19
JP2008545412A (en) 2008-12-18
US20110196343A1 (en) 2011-08-11
US8506955B2 (en) 2013-08-13

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