WO2006133182A3 - Insect-specific protease recognition sequences - Google Patents

Insect-specific protease recognition sequences Download PDF

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Publication number
WO2006133182A3
WO2006133182A3 PCT/US2006/021902 US2006021902W WO2006133182A3 WO 2006133182 A3 WO2006133182 A3 WO 2006133182A3 US 2006021902 W US2006021902 W US 2006021902W WO 2006133182 A3 WO2006133182 A3 WO 2006133182A3
Authority
WO
WIPO (PCT)
Prior art keywords
insect
modified
toxin
gut
activation site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/021902
Other languages
French (fr)
Other versions
WO2006133182A2 (en
Inventor
Andre R Abad
Gary C Chun
Ronald D Flannagan
Rafael Herrmann
Albert L Lu
Billy F Mccutchen
Janet A Rice
Eric J Schepers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
EIDP Inc
Original Assignee
Pioneer Hi Bred International Inc
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc, EI Du Pont de Nemours and Co filed Critical Pioneer Hi Bred International Inc
Priority to CA2611539A priority Critical patent/CA2611539C/en
Priority to BRPI0611681-7A priority patent/BRPI0611681B1/en
Priority to MX2007015538A priority patent/MX2007015538A/en
Priority to AT06772278T priority patent/ATE519778T1/en
Priority to EP06772278A priority patent/EP1893638B1/en
Publication of WO2006133182A2 publication Critical patent/WO2006133182A2/en
Publication of WO2006133182A3 publication Critical patent/WO2006133182A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Insects & Arthropods (AREA)
  • Agronomy & Crop Science (AREA)
  • Physics & Mathematics (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
PCT/US2006/021902 2005-06-08 2006-06-06 Insect-specific protease recognition sequences Ceased WO2006133182A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2611539A CA2611539C (en) 2005-06-08 2006-06-06 Insect-specific protease recognition sequences
BRPI0611681-7A BRPI0611681B1 (en) 2005-06-08 2006-06-06 ISOLATED NUCLEIC ACID MOLECULES, EXPRESSION CASSETTE, METHODS FOR PROTECTING AN INSECT PEST PLANT, PROCESSED PLANT METHOD, ISOLATED PROTOXIN, COMPOSITION, IMPACT METHOD
MX2007015538A MX2007015538A (en) 2005-06-08 2006-06-06 SEQUENCES OF RECOGNITION OF SPECIFIC PROTEASES FOR INSECTS.
AT06772278T ATE519778T1 (en) 2005-06-08 2006-06-06 INSECT-SPECIFIC PROTEASE DETECTION SEQUENCES
EP06772278A EP1893638B1 (en) 2005-06-08 2006-06-06 Insect-specific protease recognition sequences

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US68863505P 2005-06-08 2005-06-08
US60/688,635 2005-06-08
US72278705P 2005-09-30 2005-09-30
US60/722,787 2005-09-30

Publications (2)

Publication Number Publication Date
WO2006133182A2 WO2006133182A2 (en) 2006-12-14
WO2006133182A3 true WO2006133182A3 (en) 2007-02-08

Family

ID=37142618

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/021902 Ceased WO2006133182A2 (en) 2005-06-08 2006-06-06 Insect-specific protease recognition sequences

Country Status (8)

Country Link
US (1) US7473821B2 (en)
EP (1) EP1893638B1 (en)
AR (1) AR053403A1 (en)
AT (1) ATE519778T1 (en)
BR (1) BRPI0611681B1 (en)
CA (1) CA2611539C (en)
MX (1) MX2007015538A (en)
WO (1) WO2006133182A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
WO2005063996A2 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Plant activation of insect toxin
US8609936B2 (en) 2007-04-27 2013-12-17 Monsanto Technology Llc Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis
US8486887B2 (en) * 2007-08-10 2013-07-16 The University Of Georgia Research Foundation, Inc. Enhancement of Bacillus thuringiensis Cry protein toxicities to coleopterans, and novel insect cadherin fragments
US7968525B1 (en) 2007-12-03 2011-06-28 University Of Florida Research Foundation, Inc. Use of RNA interference to validate new termiticide target sites and a method of termite control
MX2010007416A (en) * 2008-01-10 2010-08-16 Pioneer Hi Bred Int Novel bacillus thuringiensis gene with coleopteran activity.
US8269069B1 (en) 2008-07-30 2012-09-18 Dow Agrosciences, Llc Modified Bacillus thuringiensis cry proteins that inhibit coleopterans
US20110214208A1 (en) * 2008-08-25 2011-09-01 Dow Agrosciences Llc Modified Bacillus Thuringiensis Cry5 Proteins For Nematode Control
US20110239334A1 (en) * 2008-08-25 2011-09-29 Dow Agrosciences Llc Nematode-resistant plants, and modified bacillus thuringiensis cry genes and proteins
BRPI0917185A2 (en) * 2008-08-25 2015-07-28 Dow Agrosciences Llc Bacillus thuringiensis cry12 proteins modified for nematode control
BRPI0917140A2 (en) * 2008-08-25 2015-07-28 Dow Agrosciences Llc Modified cry6 bacillus thuringiensis proteins for nematode control
WO2011009182A2 (en) 2009-07-24 2011-01-27 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Isolated nucleic acid molecule, genetic construct, vector, transgenic cell, method for producing a transgenic cell and plant, isolated and purified polypeptide, biodegradable pesticide composition, pest control method, method for producing transgenic strains resistant to insect pests
PH12012501429B1 (en) * 2009-12-16 2020-02-05 Dow Agrosciences Llc Modified cry1ca insecticidal cry proteins
BR112014024861B1 (en) 2012-04-06 2021-11-16 Monsanto Technology Llc INSECT INHIBITOR POLYPEPTIDE, POLYNUCLEOTIDE ENCODING SAME, BACTERIAL HOST CELL, INSECT INHIBITORY COMPOSITION, GOODS AND METHODS OF CONTROLLING A HEMIPTERA PEST AND MAKING GOODS
CN104812901B (en) * 2012-09-13 2018-08-10 美国印第安纳大学研究和技术公司 The composition and system and its application method of conferring disease resistance in plants
CN108064298A (en) 2014-09-11 2018-05-22 马罗内生物创新公司 Color bacillus (CHROMOBACTERIUM SUBTSUGAE) genome under Chinese hemlock spruce
US10612037B2 (en) 2016-06-20 2020-04-07 Monsanto Technology Llc Insecticidal proteins toxic or inhibitory to hemipteran pests
AR117409A1 (en) * 2018-03-29 2021-08-04 Oxitec Ltd SELF-LIMITING NIGHTS
CN113610108B (en) * 2021-07-06 2022-05-20 中南民族大学 Rice pest identification method based on improved residual error network
CN118175931A (en) * 2021-10-08 2024-06-11 安道麦阿甘有限公司 Novel formulation system for carboxylic acid herbicides

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002034774A2 (en) * 2000-10-24 2002-05-02 E.I. Du Pont De Nemours And Company Bacillus thuringiensis proteins and variants thereof with pesticidal activity against coleopterans
US20030120054A1 (en) * 2001-08-31 2003-06-26 Eric Chen Modified Cry3A toxins and nucleic acid sequences coding therefor
WO2004003148A2 (en) * 2002-06-26 2004-01-08 E.I. Du Pont De Nemours And Company Genes encoding proteins with pesticidal activity
WO2005063996A2 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Plant activation of insect toxin
WO2005066349A2 (en) * 2003-12-24 2005-07-21 Pioneer Hi-Bred International, Inc. Genes encoding proteins with pesticidal activity

Family Cites Families (3)

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US5436002A (en) * 1991-01-29 1995-07-25 Mycogen Corporation Bacillus thuringiensisisolate PS201T6 toxin
US20020038005A1 (en) * 2000-01-07 2002-03-28 Wojciechowska Jana Alexandrovna Novel delta-endotoxins and nucleic acid sequences coding therefor
US20060235061A1 (en) * 2002-06-21 2006-10-19 Qlt,Inc. Methods of using benzothiophenone derivatives to treat cancer or inflammation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002034774A2 (en) * 2000-10-24 2002-05-02 E.I. Du Pont De Nemours And Company Bacillus thuringiensis proteins and variants thereof with pesticidal activity against coleopterans
US20030120054A1 (en) * 2001-08-31 2003-06-26 Eric Chen Modified Cry3A toxins and nucleic acid sequences coding therefor
WO2004003148A2 (en) * 2002-06-26 2004-01-08 E.I. Du Pont De Nemours And Company Genes encoding proteins with pesticidal activity
WO2005063996A2 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Plant activation of insect toxin
WO2005066349A2 (en) * 2003-12-24 2005-07-21 Pioneer Hi-Bred International, Inc. Genes encoding proteins with pesticidal activity

Non-Patent Citations (6)

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Title
BOWN D P ET AL: "Characterisation of cysteine proteinases responsible for digestive proteolysis in guts of larval western corn rootworm (Diabrotica virgifera) by expression in the yeast Pichia pastoris", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, ELSEVIER SCIENCE LTD, GB, vol. 34, April 2004 (2004-04-01), pages 305 - 320, XP002335995, ISSN: 0965-1748 *
CRICKMORE N ET AL: "Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 62, no. 3, September 1998 (1998-09-01), pages 807 - 813, XP002132141, ISSN: 1092-2172 *
KOIWA H ET AL: "A plant defensive cystatin (soyacystatin) targets cathepsin L-like digestive cysteine proteinases (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera virgifera)", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 471, no. 1, 7 April 2000 (2000-04-07), pages 67 - 70, XP004336986, ISSN: 0014-5793 *
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Also Published As

Publication number Publication date
BRPI0611681B1 (en) 2018-01-16
EP1893638B1 (en) 2011-08-10
BRPI0611681A2 (en) 2010-09-28
WO2006133182A2 (en) 2006-12-14
CA2611539C (en) 2017-02-28
US20060288448A1 (en) 2006-12-21
US7473821B2 (en) 2009-01-06
ATE519778T1 (en) 2011-08-15
CA2611539A1 (en) 2006-12-14
MX2007015538A (en) 2008-03-06
EP1893638A2 (en) 2008-03-05
AR053403A1 (en) 2007-05-02

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