WO2007017684A3 - Ph tolerant luciferase - Google Patents

Ph tolerant luciferase Download PDF

Info

Publication number
WO2007017684A3
WO2007017684A3 PCT/GB2006/002974 GB2006002974W WO2007017684A3 WO 2007017684 A3 WO2007017684 A3 WO 2007017684A3 GB 2006002974 W GB2006002974 W GB 2006002974W WO 2007017684 A3 WO2007017684 A3 WO 2007017684A3
Authority
WO
WIPO (PCT)
Prior art keywords
luciferase
tolerant
wild
specific activity
type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2006/002974
Other languages
French (fr)
Other versions
WO2007017684A2 (en
Inventor
Laurence Carlo Tisi
Gim Hoong Erica Law
Olga Gandelman
James Augustus Henry Murray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambridge University Technical Services Ltd CUTS
Lumora Ltd
Original Assignee
Cambridge University Technical Services Ltd CUTS
Lumora Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge University Technical Services Ltd CUTS, Lumora Ltd filed Critical Cambridge University Technical Services Ltd CUTS
Priority to EP06779098A priority Critical patent/EP1924702A2/en
Priority to US11/990,228 priority patent/US20100068739A1/en
Publication of WO2007017684A2 publication Critical patent/WO2007017684A2/en
Publication of WO2007017684A3 publication Critical patent/WO2007017684A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Use of a luciferase that has a mutation of at least one amino acid selected from the group consisting of positions 14, 35, 182, 232 and 465, where the numbering is according to the sequence of the luciferase from P. pyralis (SEQ ID NO:1) in a method that is performed at a pH below the optimal pH for the wild-type luciferase during at least part of the time period over which bioluminescence measurements are taken, wherein the specific activity of the mutant luciferase is higher than the specific activity of wild-type at the pH at which the method is carried out.
PCT/GB2006/002974 2005-08-10 2006-08-09 Ph tolerant luciferase Ceased WO2007017684A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06779098A EP1924702A2 (en) 2005-08-10 2006-08-09 Ph tolerant luciferase
US11/990,228 US20100068739A1 (en) 2005-08-10 2006-08-09 PH Tolerant Luciferase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0516455.3A GB0516455D0 (en) 2005-08-10 2005-08-10 pH tolerant luciferase
GB0516455.3 2005-08-10

Publications (2)

Publication Number Publication Date
WO2007017684A2 WO2007017684A2 (en) 2007-02-15
WO2007017684A3 true WO2007017684A3 (en) 2007-05-31

Family

ID=34984408

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/002974 Ceased WO2007017684A2 (en) 2005-08-10 2006-08-09 Ph tolerant luciferase

Country Status (4)

Country Link
US (1) US20100068739A1 (en)
EP (1) EP1924702A2 (en)
GB (1) GB0516455D0 (en)
WO (1) WO2007017684A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011120559A (en) 2008-12-26 2011-06-23 Kikkoman Corp Firefly luciferase, gene thereof, and process for production of firefly luciferase
EP2525715A4 (en) * 2010-01-19 2014-06-04 Medtronic Ardian Luxembourg S R L Methods and apparatus for renal neuromodulation via stereotactic radiotherapy
US20150111918A1 (en) 2012-03-08 2015-04-23 Medtronic Ardian Luxembourg S.a.r.l Immune system neuromodulation and associated systems and methods
CN109971824A (en) * 2018-12-21 2019-07-05 安徽汇泽通环境技术有限公司 A method of utilizing the mould quick detection bio-toxicity of fluorescein
JPWO2024158050A1 (en) 2023-01-27 2024-08-02

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000024878A2 (en) * 1998-10-28 2000-05-04 The Secretary Of State For Defence Mutant luciferase having increased thermostability
US20030232404A1 (en) * 1997-09-19 2003-12-18 Promega Corporation Thermostable luciferases and methods of production
WO2004059294A2 (en) * 2002-12-23 2004-07-15 Promega Corporation Improved luciferase-based assays
WO2004062338A2 (en) * 2003-01-14 2004-07-29 Tisi Laurence C Method for determining the amount of template nucleic acid present in a sample

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030232404A1 (en) * 1997-09-19 2003-12-18 Promega Corporation Thermostable luciferases and methods of production
WO2000024878A2 (en) * 1998-10-28 2000-05-04 The Secretary Of State For Defence Mutant luciferase having increased thermostability
WO2004059294A2 (en) * 2002-12-23 2004-07-15 Promega Corporation Improved luciferase-based assays
WO2004062338A2 (en) * 2003-01-14 2004-07-29 Tisi Laurence C Method for determining the amount of template nucleic acid present in a sample

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HIROKAWA K ET AL: "Improved practical usefulness of firefly luciferase by gene chimerization and random mutagenesis", BIOCHIMICA ET BIOPHYSICA ACTA. PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 1597, no. 2, 3 June 2002 (2002-06-03), pages 271 - 279, XP004361031, ISSN: 0167-4838 *
KITAYAMA ATSUSHI ET AL: "Creation of a thermostable firefly luciferase with pH-insensitive luminescent color.", PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 77, no. 3, March 2003 (2003-03-01), pages 333 - 338, XP009080342, ISSN: 0031-8655 *
LAW G H ERICA ET AL: "Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance", BIOCHEMICAL JOURNAL, vol. 397, no. Part 2, July 2006 (2006-07-01), pages 305 - 312, XP002424746, ISSN: 0264-6021 *
TISI L C ET AL: "Development of a thermostable firefly luciferase", ANALYTICA CHIMICA ACTA, vol. 457, no. 1, 15 April 2002 (2002-04-15), pages 115 - 123, XP002424745, ISSN: 0003-2670 *

Also Published As

Publication number Publication date
US20100068739A1 (en) 2010-03-18
GB0516455D0 (en) 2005-09-14
WO2007017684A2 (en) 2007-02-15
EP1924702A2 (en) 2008-05-28

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