WO2007025097A3 - Use - Google Patents
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- Publication number
- WO2007025097A3 WO2007025097A3 PCT/US2006/033167 US2006033167W WO2007025097A3 WO 2007025097 A3 WO2007025097 A3 WO 2007025097A3 US 2006033167 W US2006033167 W US 2006033167W WO 2007025097 A3 WO2007025097 A3 WO 2007025097A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- target nucleic
- relates
- present
- transcription product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Polymers & Plastics (AREA)
- Plant Pathology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Animal Husbandry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/990,885 US10066233B2 (en) | 2005-08-26 | 2006-08-25 | Method of modulating cell resistance |
| AU2006282983A AU2006282983B2 (en) | 2005-08-26 | 2006-08-25 | Use |
| EP10169298.6A EP2336362B1 (en) | 2005-08-26 | 2006-08-25 | Use of crispr associated genes (cas) |
| NZ565563A NZ565563A (en) | 2005-08-26 | 2006-08-25 | Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell |
| CA2619833A CA2619833C (en) | 2005-08-26 | 2006-08-25 | Use of crispr associated genes (cas) |
| DK06802307.6T DK1916903T3 (en) | 2005-08-26 | 2006-08-25 | Use of CRISPR-associated genes (CAS) |
| AT06802307T ATE473637T1 (en) | 2005-08-26 | 2006-08-25 | USE OF CRISPR ASSOCIATED GENES (CAS) |
| NZ597299A NZ597299A (en) | 2005-08-26 | 2006-08-25 | Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell |
| EP17188839.9A EP3284833B1 (en) | 2005-08-26 | 2006-08-25 | Use of crispr associated genes (cas) |
| EP06802307A EP1916903B1 (en) | 2005-08-26 | 2006-08-25 | Use of crispr associated genes (cas) |
| DE602006015484T DE602006015484D1 (en) | 2005-08-26 | 2006-08-25 | USE OF CRISPR ASSOCIATED GENES (CAS) |
| US13/490,050 US9879269B2 (en) | 2005-08-26 | 2012-06-06 | Method for modulating resistance |
| US13/960,796 US20140199767A1 (en) | 2005-08-26 | 2013-08-06 | Use |
| US15/229,103 US10640778B2 (en) | 2005-08-26 | 2016-08-04 | Method of modulating cell resistance |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US71139605P | 2005-08-26 | 2005-08-26 | |
| US60/711,396 | 2005-08-26 | ||
| US74768306P | 2006-05-19 | 2006-05-19 | |
| US60/747,683 | 2006-05-19 |
Related Child Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US11/990,885 A-371-Of-International US10066233B2 (en) | 2005-08-26 | 2006-08-25 | Method of modulating cell resistance |
| US13/490,050 Continuation US9879269B2 (en) | 2005-08-26 | 2012-06-06 | Method for modulating resistance |
| US15/229,103 Continuation US10640778B2 (en) | 2005-08-26 | 2016-08-04 | Method of modulating cell resistance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| WO2007025097A2 WO2007025097A2 (en) | 2007-03-01 |
| WO2007025097A3 true WO2007025097A3 (en) | 2007-07-12 |
Family
ID=37692491
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/US2006/033167 Ceased WO2007025097A2 (en) | 2005-08-26 | 2006-08-25 | Use |
Country Status (12)
| Country | Link |
|---|---|
| US (4) | US10066233B2 (en) |
| EP (5) | EP2325332B1 (en) |
| AT (1) | ATE473637T1 (en) |
| AU (1) | AU2006282983B2 (en) |
| CA (2) | CA2961849C (en) |
| DE (1) | DE602006015484D1 (en) |
| DK (5) | DK2341149T3 (en) |
| ES (1) | ES2398918T3 (en) |
| NZ (2) | NZ597299A (en) |
| PL (1) | PL2325332T3 (en) |
| PT (1) | PT1916903E (en) |
| WO (1) | WO2007025097A2 (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9567603B2 (en) | 2013-03-15 | 2017-02-14 | The General Hospital Corporation | Using RNA-guided FokI nucleases (RFNs) to increase specificity for RNA-guided genome editing |
| US10000772B2 (en) | 2012-05-25 | 2018-06-19 | The Regents Of The University Of California | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| US10660316B2 (en) | 2016-11-04 | 2020-05-26 | Akeagen, Inc. | Genetically modified non-human animals and methods for producing heavy chain-only antibodies |
| US10765740B2 (en) | 2016-06-05 | 2020-09-08 | Snipr Technologies Limited | Selectively altering microbiota for immune modulation |
| US10920222B2 (en) | 2018-04-30 | 2021-02-16 | Snipr Biome Aps | Treating and preventing microbial infections |
| US11147830B2 (en) | 2015-05-06 | 2021-10-19 | Snipr Technologies Limited | Altering microbial populations and modifying microbiota |
| US12076375B2 (en) | 2022-06-29 | 2024-09-03 | Snipr Biome Aps | Treating and preventing E coli infections |
Families Citing this family (291)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ597299A (en) | 2005-08-26 | 2013-03-28 | Dupont Nutrition Biosci Aps | Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell |
| US9399801B2 (en) * | 2006-05-19 | 2016-07-26 | Dupont Nutrition Biosciences Aps | Tagged microorganisms and methods of tagging |
| EP2034848B1 (en) | 2006-06-16 | 2016-10-19 | DuPont Nutrition Biosciences ApS | Streptococcus thermophilus bacterium |
| PT2126130E (en) * | 2007-03-02 | 2015-08-03 | Dupont Nutrition Biosci Aps | Cultures with improved phage resistance |
| AU2013234432B2 (en) * | 2007-03-02 | 2015-09-24 | Dupont Nutrition Biosciences Aps | Cultures with improved phage resistance |
| WO2010011961A2 (en) | 2008-07-25 | 2010-01-28 | University Of Georgia Research Foundation, Inc. | Prokaryotic rnai-like system and methods of use |
| US9404098B2 (en) | 2008-11-06 | 2016-08-02 | University Of Georgia Research Foundation, Inc. | Method for cleaving a target RNA using a Cas6 polypeptide |
| WO2010054154A2 (en) | 2008-11-07 | 2010-05-14 | Danisco A/S | Bifidobacteria crispr sequences |
| US9062293B2 (en) * | 2008-12-11 | 2015-06-23 | Danisco A/S | Bacterial compositions of Staphylococcus vitulinus having nitrate reductase activity and of lactic acid bacteria and methods using these compositions |
| PL2473058T5 (en) | 2009-09-01 | 2025-04-28 | Chr. Hansen A/S | Lactic bacterium with modified galactokinase expression for texturizing food products by overexpression of exopolysaccharide |
| EP2529035B1 (en) | 2010-01-28 | 2018-03-14 | Chr. Hansen A/S | Lactic bacterium for texturizing food products selected on basis of phage resistance |
| EA024121B9 (en) | 2010-05-10 | 2017-01-30 | Дзе Реджентс Ов Дзе Юниверсити Ов Калифорния | COMPOSITIONS OF ENDORIBONUCLEAS AND METHODS OF THEIR USE |
| DK2630156T3 (en) | 2010-10-20 | 2018-12-17 | Dupont Nutrition Biosci Aps | CRISPR-CAS SEQUENCES OF LACTOCOCCUS |
| WO2012052557A1 (en) | 2010-10-22 | 2012-04-26 | Chr. Hansen A/S | Texturizing lactic acid bacteria strains |
| CN102172278B (en) * | 2011-01-21 | 2015-08-12 | 吉林紫鑫药物研究有限公司 | A kind of method preparing fruit-vegetable fermentation converting liquid |
| EP2689019A2 (en) | 2011-03-23 | 2014-01-29 | Pioneer Hi-Bred International Inc. | Methods for producing a complex transgenic trait locus |
| WO2012164565A1 (en) | 2011-06-01 | 2012-12-06 | Yeda Research And Development Co. Ltd. | Compositions and methods for downregulating prokaryotic genes |
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP2790708B1 (en) | 2011-12-16 | 2024-10-23 | Targetgene Biotechnologies Ltd. | Compositions and methods for modifying a predetermined target nucleic acid sequence |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| US9637739B2 (en) * | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| WO2013141680A1 (en) * | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
| EP3135765A1 (en) | 2012-12-06 | 2017-03-01 | Sigma-Aldrich Co. LLC | Crispr-based genome modification and regulation |
| IL239317B (en) * | 2012-12-12 | 2022-07-01 | Broad Inst Inc | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
| WO2014093701A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| ES2576126T3 (en) * | 2012-12-12 | 2016-07-05 | The Broad Institute, Inc. | Modification by genetic technology and optimization of improved enzyme systems, methods and compositions for sequence manipulation |
| DK2825654T3 (en) * | 2012-12-12 | 2017-08-21 | Broad Inst Inc | SYSTEMS, PROCEDURES, AND COMPOSITIONS WITH CRISPR-CAS COMPONENTS FOR SEQUENCE MANIPULATION. |
| EP3434776A1 (en) | 2012-12-12 | 2019-01-30 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| SG10201912991WA (en) | 2012-12-17 | 2020-03-30 | Harvard College | Rna-guided human genome engineering |
| EP3919505B1 (en) | 2013-01-16 | 2023-08-30 | Emory University | Uses of cas9-nucleic acid complexes |
| US10660943B2 (en) | 2013-02-07 | 2020-05-26 | The Rockefeller University | Sequence specific antimicrobials |
| US11135273B2 (en) | 2013-02-07 | 2021-10-05 | The Rockefeller University | Sequence specific antimicrobials |
| ES2522765B2 (en) * | 2013-02-22 | 2015-03-18 | Universidad De Alicante | Method to detect spacer insertions in CRISPR structures |
| US10612043B2 (en) | 2013-03-09 | 2020-04-07 | Agilent Technologies, Inc. | Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events |
| UA123532C2 (en) | 2013-03-12 | 2021-04-21 | Е. І. Дю Пон Де Немур Енд Компані | Methods for the identification of variant recognition sites for rare-cutting engineered double-strand-break-inducing agents and compositions and uses thereof |
| US9803214B2 (en) | 2013-03-12 | 2017-10-31 | Pioneer Hi-Bred International, Inc. | Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility |
| SG11201507378UA (en) | 2013-03-14 | 2015-10-29 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| JP2016522679A (en) * | 2013-04-04 | 2016-08-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Therapeutic use of genome editing with the CRISPR / Cas system |
| US9902973B2 (en) | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
| JP6738729B2 (en) * | 2013-06-17 | 2020-08-12 | ザ・ブロード・インスティテュート・インコーポレイテッド | Delivery, engineering and optimization of systems, methods and compositions for targeting and modeling postmitotic cell diseases and disorders |
| CN105793425B (en) * | 2013-06-17 | 2021-10-26 | 布罗德研究所有限公司 | Delivery, use and therapeutic applications of CRISPR-CAS systems and compositions for targeting disorders and diseases using viral components |
| JP6665088B2 (en) | 2013-06-17 | 2020-03-13 | ザ・ブロード・インスティテュート・インコーポレイテッド | Optimized CRISPR-Cas double nickase system, method and composition for sequence manipulation |
| MX2015017312A (en) | 2013-06-17 | 2017-04-10 | Broad Inst Inc | SUPPLY AND USE OF CRISPR-CAS COMPOSITIONS, VECTORS AND SYSTEMS FOR DIRECTED MODIFICATION AND HEPATIC THERAPY. |
| WO2014204724A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
| EP3725885A1 (en) | 2013-06-17 | 2020-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| JP2016528890A (en) | 2013-07-09 | 2016-09-23 | プレジデント アンド フェローズ オブ ハーバード カレッジ | Therapeutic use of genome editing using the CRISPR / Cas system |
| CN103388006B (en) * | 2013-07-26 | 2015-10-28 | 华东师范大学 | A kind of construction process of site-directed point mutation |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
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| CA3221516A1 (en) | 2013-08-22 | 2015-02-26 | E. I. Du Pont De Nemours And Company | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
| US10760065B2 (en) | 2013-09-05 | 2020-09-01 | Massachusetts Institute Of Technology | Tuning microbial populations with programmable nucleases |
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| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| EP4063503A1 (en) | 2014-02-11 | 2022-09-28 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
| US10150985B2 (en) | 2014-02-13 | 2018-12-11 | Takara Bio Usa, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| CN106536739B (en) * | 2014-04-14 | 2021-08-03 | 内梅西斯生物有限公司 | therapeutic agent |
| EP3919621A1 (en) | 2014-06-23 | 2021-12-08 | The General Hospital Corporation | Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq) |
| CN106687594A (en) | 2014-07-11 | 2017-05-17 | 纳幕尔杜邦公司 | Compositions and methods for producing plants resistant to glyphosate herbicide |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US20170218384A1 (en) | 2014-08-08 | 2017-08-03 | Pioneer Hi-Bred International, Inc. | Ubiquitin promoters and introns and methods of use |
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