WO2007033385A3 - Microfluidic assay devices and methods - Google Patents

Microfluidic assay devices and methods Download PDF

Info

Publication number
WO2007033385A3
WO2007033385A3 PCT/US2006/036365 US2006036365W WO2007033385A3 WO 2007033385 A3 WO2007033385 A3 WO 2007033385A3 US 2006036365 W US2006036365 W US 2006036365W WO 2007033385 A3 WO2007033385 A3 WO 2007033385A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
assay devices
microfluidic assay
microfluidic
talk
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/036365
Other languages
French (fr)
Other versions
WO2007033385A2 (en
Inventor
Hany Ramez Nassef
Hou-Pu Chou
Michael Lucero
Andrew May
Kathy Yokobata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Standard Biotools Inc
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Priority to EP20060803816 priority Critical patent/EP1938101A2/en
Publication of WO2007033385A2 publication Critical patent/WO2007033385A2/en
Publication of WO2007033385A3 publication Critical patent/WO2007033385A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0003—Constructional types of microvalves; Details of the cutting-off member
    • F16K99/0015—Diaphragm or membrane valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0034—Operating means specially adapted for microvalves
    • F16K99/0042—Electric operating means therefor
    • F16K99/0051—Electric operating means therefor using electrostatic means
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K99/0001—Microvalves
    • F16K99/0034—Operating means specially adapted for microvalves
    • F16K99/0055—Operating means specially adapted for microvalves actuated by fluids
    • F16K99/0059—Operating means specially adapted for microvalves actuated by fluids actuated by a pilot fluid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00389—Feeding through valves
    • B01J2219/00396—Membrane valves
    • B01J2219/00398—Membrane valves in multiple arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/0074—Fabrication methods specifically adapted for microvalves using photolithography, e.g. etching
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/008—Multi-layer fabrications
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K99/00—Subject matter not provided for in other groups of this subclass
    • F16K2099/0082—Microvalves adapted for a particular use
    • F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Mechanical Engineering (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.
PCT/US2006/036365 2005-09-13 2006-09-13 Microfluidic assay devices and methods Ceased WO2007033385A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20060803816 EP1938101A2 (en) 2005-09-13 2006-09-13 Microfluidic assay devices and methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71682305P 2005-09-13 2005-09-13
US60/716,823 2005-09-13

Publications (2)

Publication Number Publication Date
WO2007033385A2 WO2007033385A2 (en) 2007-03-22
WO2007033385A3 true WO2007033385A3 (en) 2007-07-12

Family

ID=37663179

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/036365 Ceased WO2007033385A2 (en) 2005-09-13 2006-09-13 Microfluidic assay devices and methods

Country Status (3)

Country Link
US (2) US20070074972A1 (en)
EP (1) EP1938101A2 (en)
WO (1) WO2007033385A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use

Families Citing this family (133)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US8052792B2 (en) 2001-04-06 2011-11-08 California Institute Of Technology Microfluidic protein crystallography techniques
US7459022B2 (en) 2001-04-06 2008-12-02 California Institute Of Technology Microfluidic protein crystallography
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
US7306672B2 (en) 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US20050118073A1 (en) 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
EP1343973B2 (en) 2000-11-16 2020-09-16 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
JP2006501056A (en) 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー Micro fluid large scale integration
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
AU2004240944A1 (en) 2003-05-20 2004-12-02 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
US7407799B2 (en) 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
CN103884698B (en) 2004-06-07 2017-04-12 先锋生物科技股份有限公司 Optical lens system and method for microfluidic devices
EP1838431A4 (en) 2004-12-03 2012-08-22 California Inst Of Techn MICROFLUIDIC DEVICES WITH CIRCUITS FOR CHEMICAL REACTIONS
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
US7883669B2 (en) 2005-04-20 2011-02-08 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
EP1938101A2 (en) 2005-09-13 2008-07-02 Fluidigm Corporation Microfluidic assay devices and methods
US20070095407A1 (en) * 2005-10-28 2007-05-03 Academia Sinica Electrically controlled addressable multi-dimensional microfluidic device and method
WO2007081385A2 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US8492098B2 (en) * 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
CA2659745A1 (en) * 2006-08-02 2008-02-07 California Institute Of Technology Methods and systems for detecting and/or sorting targets
US8055034B2 (en) 2006-09-13 2011-11-08 Fluidigm Corporation Methods and systems for image processing of microfluidic devices
EP2078199B1 (en) * 2006-10-12 2013-03-06 Koninklijke Philips Electronics N.V. Magnetic and/or electric label assisted detection system and method
US8473216B2 (en) 2006-11-30 2013-06-25 Fluidigm Corporation Method and program for performing baseline correction of amplification curves in a PCR experiment
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US20080243865A1 (en) * 2007-03-28 2008-10-02 Oracle International Corporation Maintaining global state of distributed transaction managed by an external transaction manager for clustered database systems
US7906074B2 (en) 2007-04-09 2011-03-15 Auburn University Microfluidic array system for biological, chemical, and biochemical assessments
EP2167633A4 (en) 2007-07-16 2014-12-24 California Inst Of Techn MICROFLUIDIC DEVICES, METHODS AND SYSTEMS FOR DETECTING TARGET MOLECULES
US8016260B2 (en) * 2007-07-19 2011-09-13 Formulatrix, Inc. Metering assembly and method of dispensing fluid
US8545773B2 (en) * 2007-07-25 2013-10-01 The Board Of Trustees Of The University Of Arkansas Versatile multichannel capillary biosensor system
JP5503540B2 (en) * 2007-08-30 2014-05-28 トラスティーズ・オブ・タフツ・カレッジ Method for determining analyte concentration in solution
JP5641184B2 (en) * 2007-09-10 2014-12-17 日本電気株式会社 Microchip sample processing equipment
US8623294B2 (en) * 2008-03-24 2014-01-07 Nec Corporation Flow passage control mechanism for microchip
WO2009126826A1 (en) * 2008-04-11 2009-10-15 Fluidigm Corporation Multilevel microfluidic systems and methods
US8961902B2 (en) 2008-04-23 2015-02-24 Bioscale, Inc. Method and apparatus for analyte processing
US8122901B2 (en) 2008-06-30 2012-02-28 Canon U.S. Life Sciences, Inc. System and method for microfluidic flow control
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
CN104043490A (en) 2008-07-25 2014-09-17 弗卢丁公司 Method and system for manufacturing integrated fluidic chips
WO2010017210A1 (en) 2008-08-07 2010-02-11 Fluidigm Corporation Microfluidic mixing and reaction systems for high efficiency screening
JP5781436B2 (en) 2008-08-11 2015-09-24 バンヤン・バイオマーカーズ・インコーポレーテッド Biomarker detection methods and assays for neurological conditions
US20140342381A1 (en) * 2008-08-11 2014-11-20 Banyan Biomarkers, Inc. Devices and methods for biomarker detection process and assay of neurological condition
US12601749B2 (en) 2008-08-11 2026-04-14 Banyan Biomarkers, Inc. Biomarker detection process and assay of neurological condition
US20100075862A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US20100075439A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification
US8222047B2 (en) * 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
EP3572796B8 (en) 2008-10-27 2023-01-18 Genalyte, Inc. Biosensors based on optical probing and sensing
CN101629143B (en) * 2008-12-02 2011-09-21 中国科学院上海微系统与信息技术研究所 Microfluidic cell array chip, method and application for high-throughput drug screening
CN102281950B (en) * 2008-12-08 2015-05-06 富鲁达公司 Programmable microfluidic digital array
US8058630B2 (en) 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods
US8100293B2 (en) * 2009-01-23 2012-01-24 Formulatrix, Inc. Microfluidic dispensing assembly
SG174617A1 (en) 2009-04-02 2011-10-28 Fluidigm Corp Multi-primer amplification method for barcoding of target nucleic acids
WO2011011669A1 (en) * 2009-07-23 2011-01-27 University Of Wyoming Methods and compositions for detection of biological materials using microfluidic devices
US8551787B2 (en) 2009-07-23 2013-10-08 Fluidigm Corporation Microfluidic devices and methods for binary mixing
CN102597774B (en) 2009-09-23 2015-05-13 皇家飞利浦电子股份有限公司 Binding assays utilizing multiple magnetic labels to track binding reagents
SG169918A1 (en) 2009-10-02 2011-04-29 Fluidigm Corp Microfluidic devices with removable cover and methods of fabrication and application
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
WO2012071069A1 (en) * 2010-11-23 2012-05-31 Cyvek, Inc. Method and apparatus for performing assays
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
US9651568B2 (en) 2009-11-23 2017-05-16 Cyvek, Inc. Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
WO2011066588A1 (en) * 2009-11-30 2011-06-03 Fluidigm Corporation Microfluidic device regeneration
US9188243B2 (en) 2010-01-29 2015-11-17 The Trustees Of Columbia University In The City Of New York Microfluidic flow devices, methods and systems
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US9678068B2 (en) * 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
CN103026232B (en) 2010-03-01 2015-02-04 匡特里克斯公司 Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US20110312537A1 (en) 2010-06-17 2011-12-22 Geneasys Pty Ltd Loc device for amplifying and detecting target nucleic acid sequences using electrochemiluminescent resonant energy transfer, linear probes with covalently attached primers
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
WO2012054933A2 (en) 2010-10-22 2012-04-26 Fluidigm Corporation Universal probe assay methods
US8975087B2 (en) 2010-11-24 2015-03-10 Inanovate, Inc. Longitudinal assay
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
CN103765224A (en) * 2011-03-10 2014-04-30 生物检测仪器有限公司 Kit-based systems and methods for detecting analytes in samples
US9168531B2 (en) 2011-03-24 2015-10-27 Fluidigm Corporation Method for thermal cycling of microfluidic samples
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
US8809238B2 (en) 2011-05-09 2014-08-19 Fluidigm Corporation Probe based nucleic acid detection
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
JP6018636B2 (en) 2011-09-30 2016-11-02 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ Multi-channel flow cell
AU2013204332B2 (en) 2012-04-16 2015-07-16 Commonwealth Scientific And Industrial Research Organisation Methods and systems for detecting an analyte or classifying a sample
WO2013166857A1 (en) 2012-05-07 2013-11-14 Capitalbio Corporation Microfluidic devices for multi-index biochemical detection
WO2013177206A2 (en) 2012-05-21 2013-11-28 Fluidigm Corporation Single-particle analysis of particle populations
EP4148118A1 (en) 2012-08-24 2023-03-15 Yale University System, device and method for high-throughput multi-plexed detection
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
WO2014150860A1 (en) * 2013-03-15 2014-09-25 Inanovate, Inc. Analysis methods of time-resolved assay data
WO2014150869A1 (en) * 2013-03-15 2014-09-25 Inanovate, Inc. Cartridge device for processing time-resolved assays
JP6625519B2 (en) * 2013-03-15 2019-12-25 イナノベイト， インコーポレイテッド Assay systems and cartridge devices
PT106942B (en) * 2013-05-15 2020-09-10 STAB VIDA, Investigação e Serviços em Ciências Biológicas, Lda BIOCHIP (1) AND BOUQUET OF 16 ANTIGENS (2) FOR DETECTION OF ACUTE AND CHRONIC LYME DISEASE
US9724692B2 (en) 2013-06-27 2017-08-08 Quark Biosciences, Inc. Multiplex slide plate
US10415084B2 (en) 2013-06-27 2019-09-17 Quark Biosciences Taiwan, Inc. Multiplex slide plate device and operation method thereof
US20160258005A1 (en) * 2013-07-02 2016-09-08 The Trustees Of The University Of Pennsylvania Methods for rapid ribonucleic acid fluorescence in situ hybridization
WO2015009688A1 (en) * 2013-07-17 2015-01-22 The Johns Hopkins University Microfluidic chip for analysis of cell motility and methods for using same
US10584366B2 (en) 2014-12-03 2020-03-10 IsoPlexis Corporation Analysis and screening of cell secretion profiles
WO2016116790A1 (en) * 2015-01-22 2016-07-28 Kwazulu-Natal Research Institute For Tb-Hiv (K-Rith) Npc Microdialysis device
US11353448B2 (en) 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
WO2017027838A1 (en) * 2015-08-13 2017-02-16 President And Fellows Of Harvard College Microfluidic devices and systems for cell culture and/or assay
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
CN108603224B (en) 2015-12-16 2022-06-28 富鲁达公司 High level multiplexed amplification
JP6784970B2 (en) * 2016-08-02 2020-11-18 国立研究開発法人産業技術総合研究所 Fluid control mechanism and kit for immunochromatographic analysis using it
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
US11078298B2 (en) 2016-10-28 2021-08-03 Banyan Biomarkers, Inc. Antibodies to ubiquitin C-terminal hydrolase L1 (UCH-L1) and glial fibrillary acidic protein (GFAP) and related methods
EP3538891B1 (en) 2016-11-11 2022-01-05 Isoplexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
PL3538665T3 (en) 2016-11-14 2024-05-06 PPB Technology Pty Ltd Protease sensor molecules
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
CN109201128A (en) 2017-07-05 2019-01-15 京东方科技集团股份有限公司 Micro-fluidic chip, chemiluminescence immunoassay system and analysis method
AR112932A1 (en) 2017-08-08 2020-01-08 Commw Scient Ind Res Org CARBOHYDRATE SENSORS
US11402376B2 (en) 2018-03-23 2022-08-02 University Of Wyoming Methods and devices for detection of biological materials using electric field assisted rapid analyte capture
PL425106A1 (en) * 2018-03-30 2019-10-07 Bacteromic Spółka Z Ograniczoną Odpowiedzialnością Microflow chip
PL425107A1 (en) 2018-03-30 2019-10-07 Bacteromic Spółka Z Ograniczoną Odpowiedzialnością Incubation section
CN108872081B (en) * 2018-09-04 2023-06-23 重庆科技学院 Multilayer microfluidic chip for detecting heavy metal ions
RU200301U1 (en) * 2019-05-31 2020-10-15 Общество с ограниченной ответственностью "ИНФРАКАП-МЕДИЦИНА" MICROFLUID CHIP FOR MULTI-PARAMETRIC IMMUNO ASSAY
CN110346585A (en) * 2019-07-10 2019-10-18 深圳金迈隆电子技术有限公司 A kind of on piece laboratory testing method and system
WO2021202929A1 (en) * 2020-04-02 2021-10-07 Genalyte, Inc. Rapid multiplexed serological test
WO2022051570A1 (en) * 2020-09-07 2022-03-10 Berkeley Lights, Inc. Methods of assaying a biological cell
US20250011852A1 (en) * 2021-09-28 2025-01-09 The Regents Of The University Of California Methods and devices for using airflow-driven, evaporative gradients
US20240424491A1 (en) * 2021-10-21 2024-12-26 Agency For Science, Technology And Research Microfluidic chip for sample preparation and sample preparation system including same
CN118059969B (en) * 2024-01-11 2025-09-12 杭州联川生物技术股份有限公司 Oscillation method for reagent oscillation using a microfluidic chip

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001007889A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO2001008799A1 (en) * 1999-08-01 2001-02-08 Febit Ferrarius Biotechnology Gmbh Microfluid reaction carrier having three flow levels and a transparent protective layer
DE10041853C1 (en) * 2000-08-25 2002-02-28 Gmd Gmbh Configurable microreactor network
US20050047967A1 (en) * 2003-09-03 2005-03-03 Industrial Technology Research Institute Microfluidic component providing multi-directional fluid movement
US20050129581A1 (en) * 2003-04-03 2005-06-16 Fluidigm Corporation Microfluidic devices and methods of using same

Family Cites Families (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US7160687B1 (en) * 1997-05-29 2007-01-09 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6833242B2 (en) 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US7214298B2 (en) 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
US6540896B1 (en) * 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
US6958865B1 (en) 1998-11-12 2005-10-25 California Institute Of Technology Microlicensing particles and applications
US7306672B2 (en) 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7459022B2 (en) 2001-04-06 2008-12-02 California Institute Of Technology Microfluidic protein crystallography
WO2001067369A2 (en) 2000-03-03 2001-09-13 California Institute Of Technology Combinatorial array for nucleic acid analysis
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US20050118073A1 (en) 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6885982B2 (en) 2000-06-27 2005-04-26 Fluidigm Corporation Object oriented microfluidic design method and system
US7062418B2 (en) 2000-06-27 2006-06-13 Fluidigm Corporation Computer aided design method and system for developing a microfluidic system
US6301055B1 (en) 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US7097809B2 (en) 2000-10-03 2006-08-29 California Institute Of Technology Combinatorial synthesis system
AU2002211389A1 (en) 2000-10-03 2002-04-15 California Institute Of Technology Microfluidic devices and methods of use
US7678547B2 (en) 2000-10-03 2010-03-16 California Institute Of Technology Velocity independent analyte characterization
EP1336097A4 (en) 2000-10-13 2006-02-01 Fluidigm Corp SAMPLE INJECTION SYSTEM USING A MICROFLUIDIC DEVICE, FOR ANALYSIS DEVICES
US7232109B2 (en) 2000-11-06 2007-06-19 California Institute Of Technology Electrostatic valves for microfluidic devices
EP1343973B2 (en) 2000-11-16 2020-09-16 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
AU2002248149A1 (en) 2000-11-16 2002-08-12 Fluidigm Corporation Microfluidic devices for introducing and dispensing fluids from microfluidic systems
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
WO2002081183A1 (en) 2001-04-06 2002-10-17 Fluidigm Corporation Polymer surface modification
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES
US6802342B2 (en) 2001-04-06 2004-10-12 Fluidigm Corporation Microfabricated fluidic circuit elements and applications
US7075162B2 (en) 2001-08-30 2006-07-11 Fluidigm Corporation Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes
WO2003031163A2 (en) 2001-10-08 2003-04-17 California Institute Of Technology Microfabricated lenses, methods of manufacture thereof, and applications therefor
US7192629B2 (en) 2001-10-11 2007-03-20 California Institute Of Technology Devices utilizing self-assembled gel and method of manufacture
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
WO2003048295A1 (en) 2001-11-30 2003-06-12 Fluidigm Corporation Microfluidic device and methods of using same
AU2003224817B2 (en) 2002-04-01 2008-11-06 Fluidigm Corporation Microfluidic particle-analysis systems
US6864951B1 (en) * 2002-05-08 2005-03-08 University Of Central Florida Tunable electronic lens and prisms using inhomogeneous nano scale liquid crystal droplets
US7059348B2 (en) 2002-05-13 2006-06-13 Fluidigm Corporation Drug delivery system
US20060086309A1 (en) 2002-06-24 2006-04-27 Fluiding Corporation Recirculating fluidic network and methods for using the same
JP2006501056A (en) * 2002-09-25 2006-01-12 カリフォルニア インスティテュート オブ テクノロジー Micro fluid large scale integration
WO2004040001A2 (en) 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
GB0302302D0 (en) 2003-01-31 2003-03-05 Glaxo Group Ltd Microfluidic apparatus and method
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
WO2004094020A2 (en) 2003-04-17 2004-11-04 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
US7366163B1 (en) * 2003-04-25 2008-04-29 At&T Corp. Method for providing local and toll services with LNP, and toll-free services to a calling party which originates the call from an IP location connected to a sip-enabled IP network
AU2004240944A1 (en) 2003-05-20 2004-12-02 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
AU2004261655A1 (en) 2003-07-28 2005-02-10 Fluidigm Corporation Image processing method and system for microfluidic devices
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7042649B2 (en) 2003-08-11 2006-05-09 California Institute Of Technology Microfabricated rubber microscope using soft solid immersion lenses
US20060172408A1 (en) 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
US7407799B2 (en) 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
CN103884698B (en) 2004-06-07 2017-04-12 先锋生物科技股份有限公司 Optical lens system and method for microfluidic devices
EP1838431A4 (en) 2004-12-03 2012-08-22 California Inst Of Techn MICROFLUIDIC DEVICES WITH CIRCUITS FOR CHEMICAL REACTIONS
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
US7883669B2 (en) 2005-04-20 2011-02-08 Fluidigm Corporation Analysis engine and database for manipulating parameters for fluidic systems on a chip
WO2007044091A2 (en) 2005-06-02 2007-04-19 Fluidigm Corporation Analysis using microfluidic partitioning devices
US20070054293A1 (en) 2005-08-30 2007-03-08 California Institute Of Technology Microfluidic chaotic mixing systems and methods
EP1938101A2 (en) 2005-09-13 2008-07-02 Fluidigm Corporation Microfluidic assay devices and methods
US8206975B2 (en) 2005-10-28 2012-06-26 California Institute Of Technology Method and device for regulating fluid flow in microfluidic devices
US20070248971A1 (en) 2006-01-26 2007-10-25 California Institute Of Technology Programming microfluidic devices with molecular information
WO2007089587A2 (en) 2006-01-26 2007-08-09 California Institute Of Technology Mechanically induced trapping of molecular interactions
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US8828661B2 (en) 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
US8055034B2 (en) 2006-09-13 2011-11-08 Fluidigm Corporation Methods and systems for image processing of microfluidic devices
EP2074341A4 (en) 2006-10-04 2013-04-10 Fluidigm Corp MICROFLUIDIC NO-RETURN VALVES
US8473216B2 (en) 2006-11-30 2013-06-25 Fluidigm Corporation Method and program for performing baseline correction of amplification curves in a PCR experiment
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US7974380B2 (en) 2007-05-09 2011-07-05 Fluidigm Corporation Method and system for crystallization and X-ray diffraction screening
JP4488031B2 (en) * 2007-06-14 2010-06-23 株式会社デンソー Air flow measurement device
WO2009033178A1 (en) 2007-09-07 2009-03-12 Fluidigm Corporation Copy number variation determination, methods and systems
WO2009100449A1 (en) 2008-02-08 2009-08-13 Fluidigm Corporation Dynamic array assay methods
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
CN104043490A (en) 2008-07-25 2014-09-17 弗卢丁公司 Method and system for manufacturing integrated fluidic chips
WO2010017210A1 (en) 2008-08-07 2010-02-11 Fluidigm Corporation Microfluidic mixing and reaction systems for high efficiency screening
CN102281950B (en) 2008-12-08 2015-05-06 富鲁达公司 Programmable microfluidic digital array
US8058630B2 (en) 2009-01-16 2011-11-15 Fluidigm Corporation Microfluidic devices and methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001007889A2 (en) * 1999-07-27 2001-02-01 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
WO2001008799A1 (en) * 1999-08-01 2001-02-08 Febit Ferrarius Biotechnology Gmbh Microfluid reaction carrier having three flow levels and a transparent protective layer
DE10041853C1 (en) * 2000-08-25 2002-02-28 Gmd Gmbh Configurable microreactor network
US20050129581A1 (en) * 2003-04-03 2005-06-16 Fluidigm Corporation Microfluidic devices and methods of using same
US20050047967A1 (en) * 2003-09-03 2005-03-03 Industrial Technology Research Institute Microfluidic component providing multi-directional fluid movement

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216412B2 (en) 2009-11-23 2015-12-22 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
US9229001B2 (en) 2009-11-23 2016-01-05 Cyvek, Inc. Method and apparatus for performing assays
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9546932B2 (en) 2009-11-23 2017-01-17 Cyvek, Inc. Microfluidic assay operating system and methods of use

Also Published As

Publication number Publication date
US20070074972A1 (en) 2007-04-05
WO2007033385A2 (en) 2007-03-22
EP1938101A2 (en) 2008-07-02
US20110020918A1 (en) 2011-01-27
US9103825B2 (en) 2015-08-11

Similar Documents

Publication Publication Date Title
WO2007033385A3 (en) Microfluidic assay devices and methods
WO2008073222A3 (en) Indirect lateral flow sandwich assay
WO2006121422A3 (en) Antibodies against clostridium difficile toxins and uses thereof
WO2008143697A3 (en) Antibody with protein a selectivity
WO2008022152A3 (en) Optimized antibodies that target cd19
WO2008140570A3 (en) Antibody with protein a selectivity
WO2009155324A3 (en) Pigf-1 assay and kits and components thereof
WO2006104989A3 (en) Altered antibody fc regions and uses thereof
WO2008122793A3 (en) Blood group antibody screening
WO2008095905A3 (en) Pathogen binding
EP2674217A3 (en) Device and process for assays using binding members
WO2008048970A3 (en) Synthetic antibodies
WO2012161288A8 (en) Immunoassay methods and reagents for decreasing nonspecific binding
WO2012038820A3 (en) Immunochromatography devices, methods and kits
WO2010096674A3 (en) A+ biomarker assays
WO2009053927A3 (en) Biologic sample assay device
WO2009014787A3 (en) Multianalyte assay
WO2007130721A3 (en) Assay for lanthanum hydroxycarbonate
WO2011056606A8 (en) Assays for detecting antibodies specific to therapeutic anti-ige antibodies and their use in anaphylaxis
WO2008076367A3 (en) Polymer-protein substrates for immunosorbent fluorescence assays
WO2006088700A3 (en) Human ring specific bnp antibodies
WO2010056889A3 (en) Use of an antibody and a rare-earth based crystal
GB2427272B (en) Immunochromatographic assay device and method
WO2006076687A3 (en) Elisa assays using prion-specific peptide reagents
WO2007014304A3 (en) Antibody profiles characteristic of tuberculosis state

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2006803816

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2006803816

Country of ref document: EP

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 06803816

Country of ref document: EP

Kind code of ref document: A2