WO2007052096A3 - Production of bone morphogenic proteins (bmps) in transgenic mammals - Google Patents

Production of bone morphogenic proteins (bmps) in transgenic mammals Download PDF

Info

Publication number
WO2007052096A3
WO2007052096A3 PCT/IB2006/001812 IB2006001812W WO2007052096A3 WO 2007052096 A3 WO2007052096 A3 WO 2007052096A3 IB 2006001812 W IB2006001812 W IB 2006001812W WO 2007052096 A3 WO2007052096 A3 WO 2007052096A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
bmps
bone morphogenic
morphogenic proteins
transgenic mammals
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2006/001812
Other languages
French (fr)
Other versions
WO2007052096A2 (en
Inventor
Cameron Malcolm Lang Clokie
Jeffrey Donald Turner
Sean Alexander Fitzgerald Peel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to EP06842193A priority Critical patent/EP1924697A2/en
Priority to CA002612479A priority patent/CA2612479A1/en
Publication of WO2007052096A2 publication Critical patent/WO2007052096A2/en
Anticipated expiration legal-status Critical
Publication of WO2007052096A3 publication Critical patent/WO2007052096A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/102—Caprine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention provides materials and methods for the production of recombinant BMPs in transgenic animals. In particular, the invention provides materials and methods for the production of recombinant BMPs in the milk of transgenic animals that express recombinant BMPs in the mammary gland.
PCT/IB2006/001812 2005-06-29 2006-06-29 Production of bone morphogenic proteins (bmps) in transgenic mammals Ceased WO2007052096A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06842193A EP1924697A2 (en) 2005-06-29 2006-06-29 Production of bone morphogenic proteins (bmps) in transgenic mammals
CA002612479A CA2612479A1 (en) 2005-06-29 2006-06-29 Production of bone morphogenic proteins (bmps) in transgenic mammals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69526305P 2005-06-29 2005-06-29
US60/695,263 2005-06-29

Publications (2)

Publication Number Publication Date
WO2007052096A2 WO2007052096A2 (en) 2007-05-10
WO2007052096A3 true WO2007052096A3 (en) 2008-02-14

Family

ID=38006236

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2006/001812 Ceased WO2007052096A2 (en) 2005-06-29 2006-06-29 Production of bone morphogenic proteins (bmps) in transgenic mammals

Country Status (4)

Country Link
US (1) US20070056050A1 (en)
EP (1) EP1924697A2 (en)
CA (1) CA2612479A1 (en)
WO (1) WO2007052096A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8193312B2 (en) 2004-01-28 2012-06-05 The Regents Of The University Of California Bone morphogenic protein binding peptide
US8188219B2 (en) 2004-01-28 2012-05-29 The Regents Of The University Of California Bone morphogenic protein binding peptide
US8415302B2 (en) * 2004-01-28 2013-04-09 The Regents Of The University Of California Surgical applications for BMP binding protein
US8753660B2 (en) * 2004-11-29 2014-06-17 The Regents Of The University Of California Activating extraction of demineralized bone matrix
WO2008144900A1 (en) * 2007-05-25 2008-12-04 Peel Sean A A method of enhancing recombinant protein production
WO2009064922A1 (en) * 2007-11-14 2009-05-22 Osteosphere, Llc Production of bone morphogenic proteins (bmps) using a novel tissue culture platform
MX2012000214A (en) 2009-06-23 2012-01-25 Univ California Enhancement of bmp retention.
HRP20210694T1 (en) 2016-07-15 2021-09-17 Acceleron Pharma, Inc. PREPARATIONS CONTAINING ACTRIIA POLYPEPTIDES FOR USE FOR THE TREATMENT OF PULMONARY HYPERTENSION
CN109242837B (en) * 2018-08-29 2021-09-14 上海市第六人民医院 Method, system, apparatus and storage medium for measuring cervical shaft angle in pelvic X-ray film
EP4142769A4 (en) 2020-04-28 2024-05-15 Acceleron Pharma Inc. ACTRII PROTEINS AND THEIR USE IN THE TREATMENT OF POST-CAPILLARY PULMONARY HYPERTENSION

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5959171A (en) * 1994-08-17 1999-09-28 Pharming B.V. Method for the production of biologically active polypeptides in a mammal's
WO1999061044A1 (en) * 1998-05-28 1999-12-02 The Board Of Trustees Of The University Of Arkansas Noggin and antagonists of bone morphogenetic proteins to suppress pathologic bone resorption
WO2001026455A1 (en) * 1999-10-14 2001-04-19 Genzyme Transgenics Corporation Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4789732A (en) * 1980-08-04 1988-12-06 Regents Of The University Of California Bone morphogenetic protein composition
US4761471A (en) * 1980-08-04 1988-08-02 The Regents Of The University Of California Bone morphogenetic protein composition
US4795804A (en) * 1983-08-16 1989-01-03 The Regents Of The University Of California Bone morphogenetic agents
US4857456A (en) * 1985-04-30 1989-08-15 The Regents Of The University Of California Assay of Bone morphogenetic protein (BMP) and anti-BMP antibody for the diagnosis of bone disorders
US6727405B1 (en) * 1986-04-09 2004-04-27 Genzyme Corporation Transgenic animals secreting desired proteins into milk
US5013649A (en) * 1986-07-01 1991-05-07 Genetics Institute, Inc. DNA sequences encoding osteoinductive products
US5631142A (en) * 1986-07-01 1997-05-20 Genetics Institute, Inc. Compositions comprising bone morphogenetic protein-2 (BMP-2)
US4877864A (en) * 1987-03-26 1989-10-31 Genetics Institute, Inc. Osteoinductive factors
US6150328A (en) * 1986-07-01 2000-11-21 Genetics Institute, Inc. BMP products
EP0279582A3 (en) * 1987-02-17 1989-10-18 Pharming B.V. Dna sequences to target proteins to the mammary gland for efficient secretion
US4873316A (en) * 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5750172A (en) * 1987-06-23 1998-05-12 Pharming B.V. Transgenic non human mammal milk
US5633076A (en) * 1989-12-01 1997-05-27 Pharming Bv Method of producing a transgenic bovine or transgenic bovine embryo
US5831141A (en) * 1991-01-11 1998-11-03 United States Of America As Represented By The Department Of Health And Human Services Expression of a heterologous polypeptide in mammary tissue of transgenic non-human mammals using a long whey acidic protein promoter
US6268545B1 (en) * 1991-06-12 2001-07-31 Institut National De La Recherche Agronomique Transgenic non-human mammal comprising a rabbit WAP promoter, uses thereof, and a DNA construct comprising the rabbit WAP promoter
ATE238417T1 (en) * 1991-11-04 2003-05-15 Inst Genetics Llc RECOMBINANT BONE MORPHOGENETIC PROTEIN HETERODIMERS, COMPOSITIONS AND METHODS OF USE
DE69434651T2 (en) * 1993-12-07 2007-03-08 Genetics Institute, Inc., Cambridge BMP-12, BMP-13 AND THESE INCLUDING SEWNE-INDUCTIVE COMPOSITIONS
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5907080A (en) * 1995-11-30 1999-05-25 Nexia Biotechnologies, Inc. Method for development of transgenic dwarf goats
EP0941310A1 (en) * 1996-10-11 1999-09-15 THE TEXAS A&M UNIVERSITY SYSTEM Methods for the generation of primordial germ cells and transgenic animal species
US20040226052A1 (en) * 2000-10-13 2004-11-11 Meade Harry M. Methods of producing a target molecule in a transgenic animal and purification of the target molecule
US6756215B1 (en) * 2000-10-20 2004-06-29 The United States Of America As Represented By The Secretary Of The Department Of Health & Human Services Functionalized TGF-β fusion proteins
DE10059336A1 (en) * 2000-11-29 2002-06-13 Scil Proteins Gmbh Production of recombinant BMP-2
US20040126375A1 (en) * 2001-01-12 2004-07-01 John Langenfeld Bone morphogenetic protein-2 in the treatment and diagnosis of cancer
AU2002243495A1 (en) * 2001-01-12 2002-07-24 University Of Medicine And Dentistry Of New Jersey Bone morphogenetic protein-2 in the treatment and diagnosis of cancer
US20030134790A1 (en) * 2002-01-11 2003-07-17 University Of Medicine And Dentistry Of New Jersey Bone Morphogenetic Protein-2 And Bone Morphogenetic Protein-4 In The Treatment And Diagnosis Of Cancer
US7459527B2 (en) * 2004-03-31 2008-12-02 Xencor, Inc. BMP-7 variants with improved properties

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5959171A (en) * 1994-08-17 1999-09-28 Pharming B.V. Method for the production of biologically active polypeptides in a mammal's
WO1999061044A1 (en) * 1998-05-28 1999-12-02 The Board Of Trustees Of The University Of Arkansas Noggin and antagonists of bone morphogenetic proteins to suppress pathologic bone resorption
WO2001026455A1 (en) * 1999-10-14 2001-04-19 Genzyme Transgenics Corporation Methods of producing a target molecule in a transgenic animal and purification of the target molecule

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
A. HOFFMANN, H. WEICH, G. GROSS, G. HILLMANN: "Perspectives in the biological function, the technical and therapeutic application of bone morphogenetic proteins", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 57, no. 3, October 2001 (2001-10-01), pages 294 - 308, XP002435759 *
GROPPE J ET AL: "STRUCTURAL BASIS OF BMP SIGNALING INHIBITION BY NOGGIN, A NOVEL TWELVE-MEMBERED CYSTINE KNOT PROTEIN", JOURNAL OF BONE AND JOINT SURGERY, JOURNAL OF BONE AND JOINT SURGERY. BOSTON, US, vol. 85, no. SUPPL 3, 2003, pages 52 - 58, XP009036882, ISSN: 0021-9355 *
GROPPE J ET AL: "STRUCTURAL BASIS OF BMP SIGNALLING INHIBITION BY THE CYSTINE KNOT PROTEIN NOGGIN", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 420, no. 6916, 12 December 2002 (2002-12-12), pages 636 - 642, XP001159617, ISSN: 0028-0836 *
PYATI UJ, WEBB AE, KIMELMAN D: "Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development.", DEVELOPMENT., vol. 132, no. 10, 13 April 2005 (2005-04-13) - May 2005 (2005-05-01), pages 2333 - 2343, XP002435761 *
VALLEJO LF, RINAS U.: "Optimized procedure for renaturation of recombinant human bone morphogenetic protein-2 at high protein concentration.", BIOTECHNOL BIOENG., vol. 85, no. 6, 20 March 2004 (2004-03-20), pages 601 - 609, XP002435760 *
YANAGITA M.: "BMP antagonists: their roles in development and involvement in pathophysiology.", CYTOKINE GROWTH FACTOR REV., vol. 16, no. 3, 1 June 2005 (2005-06-01), pages 309 - 317, XP004964511 *
ZHU W. ET AL.: "Combined morphogenetic protein -2 and 7- transfer enhances osteoblastic differentiation and spine fusion in a rodent model", JOURNAL OF BONE AND MINERAL RESEARCH, vol. 19, no. 12, 30 August 2004 (2004-08-30), XP002435758 *
ZHU W. ET AL.: "Noggin regulation of bone-morphogenetic protein ( BMP) 2/7 heterodimer activity in vitro", BONE, vol. 39, 20 February 2006 (2006-02-20), pages 61 - 71, XP002435762 *

Also Published As

Publication number Publication date
WO2007052096A2 (en) 2007-05-10
EP1924697A2 (en) 2008-05-28
US20070056050A1 (en) 2007-03-08
CA2612479A1 (en) 2007-05-10

Similar Documents

Publication Publication Date Title
CY1113199T1 (en) STABILIZED GLUCOSEREMPRODUCTION COMPOSITIONS
WO2007047112A3 (en) Anti-myostatin antibodies
PT1969007E (en) Compositions and methods for producing a composition
WO2009058379A3 (en) Protein scaffolds
WO2006017538A3 (en) Hk1-binding proteins
PT2207809E (en) Heparin-binding epidermal growth factor-like growth factor antigen binding proteins
MX389347B (en) PREVENTION OF DISULFIDE BOND REDUCTION DURING RECOMBINANT POLYPEPTIDE PRODUCTION.
WO2005014650A3 (en) Antibodies specific for sclerostin and methods for increasing bone mineralization
TW200626171A (en) Fixed dosing of HER antibodies
WO2005104835A3 (en) Transgenic animals and uses thereof
IL184235A0 (en) Monoclonal antibodies against angiopoietin-like protein 4 (angptl4)
WO2008143708A3 (en) Methods and materials related to anti-amyloid antibodies
IS2786B (en) Protein binding to Nogo receptors
WO2008011107A3 (en) Antagonists of protease activated receptor-1 (par1)
WO2007130697A3 (en) Anti-ephb4 antibodies and methods using same
WO2006132969A3 (en) Incorporation of non-naturally encoded amino acids into proteins
WO2006113767A3 (en) Hgf beta chain variants
WO2007052096A3 (en) Production of bone morphogenic proteins (bmps) in transgenic mammals
WO2007124019A3 (en) Methods and products related to the transfer of molecules from blood to the mammary gland
WO2007127506A8 (en) Anti-ephrinb2 antibodies and methods using same
WO2020065252A8 (en) Antagonists
WO2007134325A3 (en) Methods and compositions for protein production using adenoviral vectors
IL172729A0 (en) Reagents and methods for the formation of disulfide bonds and the glycosylation of proteins
EA200801180A1 (en) СЕЛЕКТИВНЫЕ ПЕПТИДНЫЕ АГОНИСТЫ РЕЦЕПТОРА VPAC2
EA200702193A1 (en) GLYCOSILIZATION BELKOV

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2612479

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 2006842193

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2006842193

Country of ref document: EP