WO2007103521A3 - Preventing norvaline and norleucine misincorporation in recombinant proteins - Google Patents

Preventing norvaline and norleucine misincorporation in recombinant proteins Download PDF

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Publication number
WO2007103521A3
WO2007103521A3 PCT/US2007/005963 US2007005963W WO2007103521A3 WO 2007103521 A3 WO2007103521 A3 WO 2007103521A3 US 2007005963 W US2007005963 W US 2007005963W WO 2007103521 A3 WO2007103521 A3 WO 2007103521A3
Authority
WO
WIPO (PCT)
Prior art keywords
norvaline
preventing
recombinant proteins
norleucine misincorporation
misincorporation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/005963
Other languages
French (fr)
Other versions
WO2007103521A2 (en
Inventor
Andrew C Macdonald
Nicholas Abu-Absi
Duane Inlow
Mary Poulhzan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Priority to EP07752647A priority Critical patent/EP1996697A2/en
Publication of WO2007103521A2 publication Critical patent/WO2007103521A2/en
Publication of WO2007103521A3 publication Critical patent/WO2007103521A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Accommodation For Nursing Or Treatment Tables (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Methods of preventing norvaline and norleucine misincorporation in recombinant proteins.
PCT/US2007/005963 2006-03-07 2007-03-07 Preventing norvaline and norleucine misincorporation in recombinant proteins Ceased WO2007103521A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07752647A EP1996697A2 (en) 2006-03-07 2007-03-07 Preventing norvaline and norleucine misincorporation in recombinant proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77942906P 2006-03-07 2006-03-07
US60/779,429 2006-03-07

Publications (2)

Publication Number Publication Date
WO2007103521A2 WO2007103521A2 (en) 2007-09-13
WO2007103521A3 true WO2007103521A3 (en) 2008-02-21

Family

ID=38475570

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/005963 Ceased WO2007103521A2 (en) 2006-03-07 2007-03-07 Preventing norvaline and norleucine misincorporation in recombinant proteins

Country Status (2)

Country Link
EP (1) EP1996697A2 (en)
WO (1) WO2007103521A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10179925B2 (en) 2012-09-19 2019-01-15 Genentech, Inc. Compositions for preventing norleucine misincorporation into proteins

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190140090A (en) 2007-07-09 2019-12-18 제넨테크, 인크. Prevention of disulfide bond reduction during recombinant production of polypeptides
KR102888586B1 (en) * 2018-12-20 2025-11-21 사노피-아벤티스 도이칠란트 게엠베하 Methods for reducing misincorporation of nonstandard branched-chain amino acids
KR20250084208A (en) 2022-10-11 2025-06-10 유씨비 바이오파마 에스알엘 Method for producing recombinant proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050037475A1 (en) * 2003-08-13 2005-02-17 Chiron Corporation Method of purifying TFPI and TFPI analogs
WO2005038017A2 (en) * 2003-09-25 2005-04-28 Monsanto Technology Llc Prevention of incorporation of non-standard amino acids into protein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050037475A1 (en) * 2003-08-13 2005-02-17 Chiron Corporation Method of purifying TFPI and TFPI analogs
WO2005038017A2 (en) * 2003-09-25 2005-04-28 Monsanto Technology Llc Prevention of incorporation of non-standard amino acids into protein

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
APOSTOL IZYDOR ET AL: "Incorporation of norvaline at leucine positions in recombinant human hemoglobin expressed in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 46, 14 November 1997 (1997-11-14), pages 28980 - 28988, XP002461320, ISSN: 0021-9258 *
BOGOSIAN G ET AL: "Biosynthesis and incorporation into protein of norleucine by Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 264, no. 1, 5 January 1989 (1989-01-05), pages 531 - 539, XP002324310, ISSN: 0021-9258 *
TSAI L B ET AL: "CONTROL OF MISINCORPORATION OF DE-NOVO SYNTHESIZED NORLEUCINE INTO RECOMBINANT INTERLEUKIN-2 ESCHERICHIA-COLI", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 156, no. 2, 1988, pages 733 - 739, XP009093276, ISSN: 0006-291X *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10179925B2 (en) 2012-09-19 2019-01-15 Genentech, Inc. Compositions for preventing norleucine misincorporation into proteins
US10421984B2 (en) 2012-09-19 2019-09-24 Genentech, Inc. Methods for reducing norleucine misincorporation into proteins using a microorganism comprising a mutant metA allele
US11015214B2 (en) 2012-09-19 2021-05-25 Genentech, Inc. Microorganisms comprising a mutant metA allele for reducing norleucine misincorporation into proteins

Also Published As

Publication number Publication date
EP1996697A2 (en) 2008-12-03
WO2007103521A2 (en) 2007-09-13

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