WO2007111847A3 - Use of additives for the reduction of non-specific binding in assays - Google Patents

Use of additives for the reduction of non-specific binding in assays Download PDF

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Publication number
WO2007111847A3
WO2007111847A3 PCT/US2007/006619 US2007006619W WO2007111847A3 WO 2007111847 A3 WO2007111847 A3 WO 2007111847A3 US 2007006619 W US2007006619 W US 2007006619W WO 2007111847 A3 WO2007111847 A3 WO 2007111847A3
Authority
WO
WIPO (PCT)
Prior art keywords
specific binding
assays
additives
reduction
reaction mixture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/006619
Other languages
French (fr)
Other versions
WO2007111847A2 (en
Inventor
Stuart Levison
Paul Nix
Derek Levison
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aokin AG
Original Assignee
Aokin AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aokin AG filed Critical Aokin AG
Priority to US12/294,293 priority Critical patent/US8124359B2/en
Priority to EP07753260A priority patent/EP2005169A2/en
Publication of WO2007111847A2 publication Critical patent/WO2007111847A2/en
Publication of WO2007111847A3 publication Critical patent/WO2007111847A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for reducing non-specific binding in an assay is provided herein. The method includes (a) providing a reaction mixture, which includes or is suspected to include a first component and a second component capable of binding to each other in a specific binding reaction, and (b) adding non-physiological amounts of at least one additive to the reaction mixture before, during or after binding in a sufficient amount to reduce non-specific binding in the reaction mixture. The method further includes (c) monitoring or measuring the presence and/or concentration of at least one of the first and second components after step (b).
PCT/US2007/006619 2006-03-24 2007-03-16 Use of additives for the reduction of non-specific binding in assays Ceased WO2007111847A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/294,293 US8124359B2 (en) 2006-03-24 2007-03-16 Use of additives for the reduction of non-specific binding in assays
EP07753260A EP2005169A2 (en) 2006-03-24 2007-03-16 Use of additives for the reduction of non-specific binding in assays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78561206P 2006-03-24 2006-03-24
US60/785,612 2006-03-24

Publications (2)

Publication Number Publication Date
WO2007111847A2 WO2007111847A2 (en) 2007-10-04
WO2007111847A3 true WO2007111847A3 (en) 2007-11-22

Family

ID=38226438

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/006619 Ceased WO2007111847A2 (en) 2006-03-24 2007-03-16 Use of additives for the reduction of non-specific binding in assays

Country Status (3)

Country Link
US (1) US8124359B2 (en)
EP (1) EP2005169A2 (en)
WO (1) WO2007111847A2 (en)

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* Cited by examiner, † Cited by third party
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US7745153B2 (en) 2008-02-06 2010-06-29 Pierce Biotechnology, Inc. Pyrocatechol violet-metal protein assay
DE102009010563A1 (en) 2009-02-16 2010-08-26 Matthias W. Engel Device for the detection of analytes in body fluids
US8486717B2 (en) 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
US8956816B2 (en) 2012-06-05 2015-02-17 Pacific Biosciences Of California, Inc. Methods and compositions for performing analytical operations
US9874556B2 (en) 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
DE102012107651A1 (en) * 2012-08-21 2014-02-27 Astrium Gmbh Method for carrying out a biochemical analysis, in particular in space
US20140072959A1 (en) 2012-09-12 2014-03-13 Force Diagnostics, Inc. Rapid tests for insurance underwriting
CN105102980B (en) 2013-02-26 2017-11-03 阿斯图特医药公司 Lateral flow assays with strip keeper
CN108051590B (en) 2013-09-13 2020-12-11 Symbolics有限责任公司 Lateral tomography detection using 2D assay and control signal readout modes
ES2740851T3 (en) * 2013-12-13 2020-02-06 Siemens Healthcare Diagnostics Inc Pretreatment agent in trials without agglutination
BR112017021305A2 (en) 2015-04-06 2018-06-26 Bludiagnostics Inc lateral flow test device, and method and kit for quantitative detection of an analyte in a saliva sample
WO2018039047A1 (en) 2016-08-23 2018-03-01 Qoolabs, Inc. Lateral flow assay for assessing recombinant protein expression or reporter gene expression
JP2019158791A (en) * 2018-03-16 2019-09-19 ウシオ電機株式会社 Immunochromatography assay method, diluent for immunochromatography, and immunochromatography test kit
CN110850078B (en) * 2019-11-14 2023-04-07 苏州和迈精密仪器有限公司 Detection method based on double-antibody sandwich double-waveband fluorescence immunity
CN116859039A (en) * 2023-07-05 2023-10-10 谱天（天津）生物科技有限公司 Blocking agent of magnetic porous material and application thereof
CN119470342A (en) * 2024-10-17 2025-02-18 中山大学 A method for accelerating analyte detection

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0155104A2 (en) * 1984-02-24 1985-09-18 AMERSHAM INTERNATIONAL plc Free analyte assay
EP0163393A2 (en) * 1984-04-05 1985-12-04 Seragen Diagnostics, Inc. A method of performing an agglutination immunoassay
EP0392332A2 (en) * 1989-04-12 1990-10-17 F. Hoffmann-La Roche Ag Fluorescence polarisation immunoassay and reagents for same
EP0535239A1 (en) * 1991-03-18 1993-04-07 Shiseido Company Limited Collagen assaying method and kit

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0155104A2 (en) * 1984-02-24 1985-09-18 AMERSHAM INTERNATIONAL plc Free analyte assay
EP0163393A2 (en) * 1984-04-05 1985-12-04 Seragen Diagnostics, Inc. A method of performing an agglutination immunoassay
EP0392332A2 (en) * 1989-04-12 1990-10-17 F. Hoffmann-La Roche Ag Fluorescence polarisation immunoassay and reagents for same
EP0535239A1 (en) * 1991-03-18 1993-04-07 Shiseido Company Limited Collagen assaying method and kit

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AOKIN: "fluorescence polarisation to quantify contamination by zearalenone (ZON)", INTERNET ARTICLE, 29 September 2006 (2006-09-29), XP002442558, Retrieved from the Internet <URL:mycoglobe.ispa.cnr.it/download.php?sezione=results&id=28> [retrieved on 20070713] *
DANDLIKER W B ET AL: "INVESTIGATION OF ANTIGEN ANTIBODY KINETICS BY FLUORESCENCE POLARIZATION", IMMUNOCHEMISTRY, vol. 5, no. 2, 1968, pages 171 - 183, XP002442557, ISSN: 0019-2791 *
LEVISON S A ET AL: "Fluorescence polarization and intensity kinetic studies of antifluorescein antibody obtained at different stages of the immune response.", BIOCHEMISTRY 26 AUG 1975, vol. 14, no. 17, 26 August 1975 (1975-08-26), pages 3778 - 3786, XP002442556, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
WO2007111847A2 (en) 2007-10-04
US20090311724A1 (en) 2009-12-17
EP2005169A2 (en) 2008-12-24
US8124359B2 (en) 2012-02-28

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