WO2007114693A3 - High throughput detection of molecular markers based on aflp and high throughput sequencing - Google Patents

High throughput detection of molecular markers based on aflp and high throughput sequencing Download PDF

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Publication number
WO2007114693A3
WO2007114693A3 PCT/NL2007/000094 NL2007000094W WO2007114693A3 WO 2007114693 A3 WO2007114693 A3 WO 2007114693A3 NL 2007000094 W NL2007000094 W NL 2007000094W WO 2007114693 A3 WO2007114693 A3 WO 2007114693A3
Authority
WO
WIPO (PCT)
Prior art keywords
high throughput
molecular markers
restriction fragments
aflp
amplified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/NL2007/000094
Other languages
French (fr)
Other versions
WO2007114693A2 (en
Inventor
Eijk Michael Josephus Ther Van
Rene Cornelis Josephus Hogers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Keygene NV
Original Assignee
Keygene NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keygene NV filed Critical Keygene NV
Priority to DK07747276.9T priority Critical patent/DK2002017T3/en
Priority to US12/296,009 priority patent/US20090253581A1/en
Priority to JP2009504137A priority patent/JP5389638B2/en
Priority to EP07747276.9A priority patent/EP2002017B1/en
Priority to EP17163116.1A priority patent/EP3239304B1/en
Priority to ES07747276.9T priority patent/ES2545264T3/en
Publication of WO2007114693A2 publication Critical patent/WO2007114693A2/en
Publication of WO2007114693A3 publication Critical patent/WO2007114693A3/en
Anticipated expiration legal-status Critical
Priority to US13/364,799 priority patent/US20120135871A1/en
Priority to US13/449,629 priority patent/US20120202698A1/en
Priority to US14/285,430 priority patent/US10023907B2/en
Priority to US16/000,252 priority patent/US20180291439A1/en
Priority to US16/516,866 priority patent/US20200181694A1/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3' end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers. The high troughput method combines the AFLP (amplified-fragment lengh polymorphism) method with high-throughput sequencing technology.
PCT/NL2007/000094 2006-04-04 2007-04-04 High throughput detection of molecular markers based on aflp and high throughput sequencing Ceased WO2007114693A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
DK07747276.9T DK2002017T3 (en) 2006-04-04 2007-04-04 High-capacity detection of molecular markers based on restriction fragments
US12/296,009 US20090253581A1 (en) 2006-04-04 2007-04-04 High Throughput Detection of Molecular Markers Based on AFLP and High Throughput Sequencing
JP2009504137A JP5389638B2 (en) 2006-04-04 2007-04-04 High-throughput detection of molecular markers based on restriction fragments
EP07747276.9A EP2002017B1 (en) 2006-04-04 2007-04-04 High throughput detection of molecular markers based on restriction fragments
EP17163116.1A EP3239304B1 (en) 2006-04-04 2007-04-04 High throughput detection of molecular markers based on aflp and high troughput sequencing
ES07747276.9T ES2545264T3 (en) 2006-04-04 2007-04-04 High performance detection of molecular markers based on restriction fragments
US13/364,799 US20120135871A1 (en) 2006-04-04 2012-02-02 High throughput detection of molecular markers based on aflp and high through-put sequencing
US13/449,629 US20120202698A1 (en) 2006-04-04 2012-04-18 High throughput detection of molecular markers based on aflp and high through-put sequencing
US14/285,430 US10023907B2 (en) 2006-04-04 2014-05-22 High throughput detection of molecular markers based on AFLP and high through-put sequencing
US16/000,252 US20180291439A1 (en) 2006-04-04 2018-06-05 High throughput detection of molecular markers based on aflp and high through-put sequencing
US16/516,866 US20200181694A1 (en) 2006-04-04 2019-07-19 High throughput detection of molecular markers based on aflp and high through-put sequencing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US78870606P 2006-04-04 2006-04-04
US60/788,706 2006-04-04
US88005207P 2007-01-12 2007-01-12
US60/880,052 2007-01-12

Related Child Applications (3)

Application Number Title Priority Date Filing Date
EP15158621.1A Previously-Filed-Application EP2963127B1 (en) 2006-04-04 2007-04-04 High throughput detection of molecular markers based on restriction fragments
US12/296,009 A-371-Of-International US20090253581A1 (en) 2006-04-04 2007-04-04 High Throughput Detection of Molecular Markers Based on AFLP and High Throughput Sequencing
US13/364,799 Continuation US20120135871A1 (en) 2006-04-04 2012-02-02 High throughput detection of molecular markers based on aflp and high through-put sequencing

Publications (2)

Publication Number Publication Date
WO2007114693A2 WO2007114693A2 (en) 2007-10-11
WO2007114693A3 true WO2007114693A3 (en) 2007-12-21

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Family Applications (1)

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PCT/NL2007/000094 Ceased WO2007114693A2 (en) 2006-04-04 2007-04-04 High throughput detection of molecular markers based on aflp and high throughput sequencing

Country Status (8)

Country Link
US (6) US20090253581A1 (en)
EP (3) EP3239304B1 (en)
JP (2) JP5389638B2 (en)
DK (2) DK3239304T3 (en)
ES (3) ES2829295T3 (en)
PL (1) PL2963127T3 (en)
PT (1) PT2963127T (en)
WO (1) WO2007114693A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
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US10450606B2 (en) 2012-02-17 2019-10-22 Fred Hutchinson Cancer Research Center Compositions and methods for accurately identifying mutations

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