WO2007119263A3 - System for acquiring 2d and 3d imagse ofsingle particles in a flow stream - Google Patents

System for acquiring 2d and 3d imagse ofsingle particles in a flow stream Download PDF

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Publication number
WO2007119263A3
WO2007119263A3 PCT/IT2007/000299 IT2007000299W WO2007119263A3 WO 2007119263 A3 WO2007119263 A3 WO 2007119263A3 IT 2007000299 W IT2007000299 W IT 2007000299W WO 2007119263 A3 WO2007119263 A3 WO 2007119263A3
Authority
WO
WIPO (PCT)
Prior art keywords
particles
cell
ingle
image capture
imagse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2007/000299
Other languages
French (fr)
Other versions
WO2007119263A2 (en
Inventor
Umberto Martinelli
Giancarlo Fausto Abate
Giuseppe Castello
Pasquale Daponte
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TECNOLOGIE BIOMEDICHE Srl Soc
TECNOLOGIE BIOMEDICHE Srl SO
Original Assignee
TECNOLOGIE BIOMEDICHE Srl Soc
TECNOLOGIE BIOMEDICHE Srl SO
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from ITCE20060008 external-priority patent/ITCE20060008A1/en
Priority claimed from ITCE20060010 external-priority patent/ITCE20060010A1/en
Priority claimed from ITCE20060009 external-priority patent/ITCE20060009A1/en
Priority claimed from ITCE20060011 external-priority patent/ITCE20060011A1/en
Application filed by TECNOLOGIE BIOMEDICHE Srl Soc, TECNOLOGIE BIOMEDICHE Srl SO filed Critical TECNOLOGIE BIOMEDICHE Srl Soc
Priority to EP07736803A priority Critical patent/EP2111539A2/en
Publication of WO2007119263A2 publication Critical patent/WO2007119263A2/en
Publication of WO2007119263A3 publication Critical patent/WO2007119263A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1027—Determining speed or velocity of a particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup
    • G01N2015/1445—Three-dimensional imaging, imaging in different image planes, e.g. under different angles or at different depths, e.g. by a relative motion of sample and detector, for instance by tomography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

An innovative opto-electronic system, which integrates in a single module, combining them simultaneously, the 2d/3d image capture functionality of single cell/ particles (tomography) carried in a laminar or non-laminar flow with the measurement possibilities of a flow-cytometer (cell dimension, granularity). The 2d/3d image capture unit of ingle particles, comprising a microscope objective and a micro- channeled measurement chamber, has been demonstrated and applied, as industrial application, in a cyto-tomographic equipment. The software for control acquisition and analysis of signal and of images originated from a ingle cell in a laminar flow has been developed with a high degree of operative implicity and reliability in the automatic data analysis, making easier the interpretative approach of the results.
PCT/IT2007/000299 2006-04-19 2007-04-19 System for acquiring 2d and 3d imagse ofsingle particles in a flow stream Ceased WO2007119263A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07736803A EP2111539A2 (en) 2006-04-19 2007-04-19 Images acquisition system in 2d and reconstructed tomographically in 3d of single cell/particles in flow carrier (laminar and non).

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
ITCE20060008 ITCE20060008A1 (en) 2006-04-19 2006-04-19 TOMOGRAPHIC SYSTEM WITH NEURAL NETWORK FOR THE ACQUISITION OF 3D IMAGE OF INDIVIDUAL CELLS AND / OR PARTICULARS IN MOVEMENT THROUGH TRANSPORT IN LAMINAR FLOW
ITCE2006A000008 2006-04-19
ITCE2006A000009 2006-04-20
ITCE2006A000010 2006-04-20
ITCE2006A000011 2006-04-20
ITCE20060010 ITCE20060010A1 (en) 2006-04-20 2006-04-20 LIGHTING SYSTEM FOR THE GENERATION AND ACQUISITION OF BIRTH IMAGES ON AND THROUGH LAMINA DI LUCE FOR TOMOGRAPHS OF CELLS AND / OR PARTICULATE IN LAMINAR FLOW.
ITCE20060009 ITCE20060009A1 (en) 2006-04-20 2006-04-20 MEASURING CHAMBER WITH MICROCELLETTE SPECIFICALLY DESIGNED FOR IMAGES OF CELLS AND / OR PARTICULATED IN LAMINAR FLOW.
ITCE20060011 ITCE20060011A1 (en) 2006-04-20 2006-04-20 SIGNAL GENERATOR OF OPTOELECTRONIC START (TRIGGER) THROUGH CLASSIFICATION OF THE EVENT AND DELAY CHECK.

Publications (2)

Publication Number Publication Date
WO2007119263A2 WO2007119263A2 (en) 2007-10-25
WO2007119263A3 true WO2007119263A3 (en) 2007-11-29

Family

ID=38515823

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2007/000299 Ceased WO2007119263A2 (en) 2006-04-19 2007-04-19 System for acquiring 2d and 3d imagse ofsingle particles in a flow stream

Country Status (2)

Country Link
EP (1) EP2111539A2 (en)
WO (1) WO2007119263A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140146157A1 (en) * 2012-11-29 2014-05-29 Fluid Imaging Technologies, Inc. System and method for sample dilution and particle imaging
JP6796917B2 (en) * 2015-09-18 2020-12-09 シスメックス株式会社 Particle imaging device and particle imaging method
US9983115B2 (en) 2015-09-21 2018-05-29 Fluid Imaging Technologies, Inc. System and method for monitoring particles in a fluid using ratiometric cytometry
WO2025236104A1 (en) * 2024-05-17 2025-11-20 Ecole Polytechnique De Montreal System and method for 3d imaging flow cytometry

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1070952A2 (en) * 1999-07-23 2001-01-24 Eauxsys (UK) Limited Method and means for particle measurement
US20020141625A1 (en) * 2001-03-28 2002-10-03 Nelson Alan C. Apparatus and method for imaging small objects in a flow stream using optical tomography
US20040260157A1 (en) * 2003-06-20 2004-12-23 Montes Miguel A. Method for automated screening of cervical/endocervical malignant and premalignant epithelial lesions using flow cytometry with HPV DNA fluorescent in-situ hybridization ( FISH) technology
WO2005050558A2 (en) * 2003-11-18 2005-06-02 University Of Washington Method and apparatus of shadowgram formation for optical tomography

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1070952A2 (en) * 1999-07-23 2001-01-24 Eauxsys (UK) Limited Method and means for particle measurement
US20020141625A1 (en) * 2001-03-28 2002-10-03 Nelson Alan C. Apparatus and method for imaging small objects in a flow stream using optical tomography
US20040260157A1 (en) * 2003-06-20 2004-12-23 Montes Miguel A. Method for automated screening of cervical/endocervical malignant and premalignant epithelial lesions using flow cytometry with HPV DNA fluorescent in-situ hybridization ( FISH) technology
WO2005050558A2 (en) * 2003-11-18 2005-06-02 University Of Washington Method and apparatus of shadowgram formation for optical tomography

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ALTENDORF E ET AL: "Differential blood cell counts obtained using a microchannel based flow cytometer", 1997 INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS AND ACTUATORS. DIGEST OF TECHNICAL PAPERS. TRANSDUCERS 97. CHICAGO, IL, JUNE 16 - 19, 1997. SESSIONS 3A1 - 4D3. PAPERS NO. 3A1.01 - 4D3.14P, INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS AND ACTU, vol. VOL. 2, 16 June 1997 (1997-06-16), pages 531 - 534, XP010240530, ISBN: 0-7803-3829-4 *
GRIMALDI D ET AL: "Hardware and software improvements in flow-cytometry measurements", MEASUREMENT, INSTITUTE OF MEASUREMENT AND CONTROL. LONDON, GB, vol. 36, no. 2, September 2004 (2004-09-01), pages 111 - 119, XP004576357, ISSN: 0263-2241 *

Also Published As

Publication number Publication date
WO2007119263A2 (en) 2007-10-25
EP2111539A2 (en) 2009-10-28

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