WO2007123961A3 - Purification processes for isolating purified vesicular stomatitis virus from cell culture - Google Patents

Purification processes for isolating purified vesicular stomatitis virus from cell culture Download PDF

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Publication number
WO2007123961A3
WO2007123961A3 PCT/US2007/009510 US2007009510W WO2007123961A3 WO 2007123961 A3 WO2007123961 A3 WO 2007123961A3 US 2007009510 W US2007009510 W US 2007009510W WO 2007123961 A3 WO2007123961 A3 WO 2007123961A3
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WO
WIPO (PCT)
Prior art keywords
vsv
cell culture
recovering
vesicular stomatitis
stomatitis virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/009510
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French (fr)
Other versions
WO2007123961A2 (en
Inventor
Yun Kang
Mark William Cutler
Amadou Affrey Ouattara
Kristen Elissa Syvertsen
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Wyeth LLC
Original Assignee
Wyeth LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP07755685A priority Critical patent/EP2007883B1/en
Priority to PL07755685T priority patent/PL2007883T3/en
Priority to CA2648792A priority patent/CA2648792C/en
Priority to CN2007800140908A priority patent/CN101426905B/en
Priority to ES07755685T priority patent/ES2377356T3/en
Priority to BRPI0710820-6A priority patent/BRPI0710820A2/en
Priority to AT07755685T priority patent/ATE539149T1/en
Priority to MX2008013494A priority patent/MX2008013494A/en
Priority to RU2008140137/10A priority patent/RU2484135C2/en
Priority to DK07755685.0T priority patent/DK2007883T3/en
Priority to SI200730829T priority patent/SI2007883T1/en
Priority to AU2007240797A priority patent/AU2007240797B2/en
Application filed by Wyeth LLC filed Critical Wyeth LLC
Priority to JP2009506566A priority patent/JP5129805B2/en
Priority to KR1020087028205A priority patent/KR101385927B1/en
Publication of WO2007123961A2 publication Critical patent/WO2007123961A2/en
Publication of WO2007123961A3 publication Critical patent/WO2007123961A3/en
Priority to IL194629A priority patent/IL194629A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N7/02—Recovery or purification
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5256—Virus expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
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    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20241—Use of virus, viral particle or viral elements as a vector
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    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20251—Methods of production or purification of viral material
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    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20211—Vesiculovirus, e.g. vesicular stomatitis Indiana virus
    • C12N2760/20251—Methods of production or purification of viral material
    • C12N2760/20252—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
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  • Animal Behavior & Ethology (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Novel purification processes for obtaining vesicular stomatitis virus (VSV) of improved purity from mammalian cell culture are described herein. More particularly, in certain embodiments, a process is described for purifying VSV from cell culture fluid of a mammalian cell culture infected with VSV, the process comprising: clarifying the cell culture fluid by low-speed centrifugation and recovering the VSV in the supernatant; filtering the supernatant through a 0.2 to 0.45 µm filter and recovering the VSV in the filtered solution; loading the VSV filtered solution onto a anion exchange membrane adsorber equilibrated with a first pH buffered salt solution, eluting the VSV from the anion exchange membrane adsorber with a second pH buffered salt solution and recovering the eluted VSV fractions; purifying the recovered VSV by tangential flow filtration (TFF) using a TFF membrane having a molecular weight cutoff between 300 kDa and 1,000 kDa and recovering the VSV in the retentate, and filtering the VSV retentate through a 0.2 to 0.22 µm filter and recovering the VSV in the filtered solution.
PCT/US2007/009510 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture Ceased WO2007123961A2 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
PL07755685T PL2007883T3 (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture
CA2648792A CA2648792C (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture
CN2007800140908A CN101426905B (en) 2006-04-20 2007-04-19 Purification method of vesicular stomatitis virus isolated and purified from cell culture
ES07755685T ES2377356T3 (en) 2006-04-20 2007-04-19 Purification procedure for the isolation of the purified vesicular stomatitis virus from a cell culture
BRPI0710820-6A BRPI0710820A2 (en) 2006-04-20 2007-04-19 process for purifying the vesicular stomatitis virus (vsv) from the cell culture fluid of a vsv infected mammalian cell culture; vsv purified according to the process; pharmaceutical composition; and immunogenic composition
AT07755685T ATE539149T1 (en) 2006-04-20 2007-04-19 PURIFICATION METHOD FOR ISOLATION OF PURIFIED VESICULAR STOMATITIS VIRUS FROM CELL CULTURE
MX2008013494A MX2008013494A (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture.
DK07755685.0T DK2007883T3 (en) 2006-04-20 2007-04-19 Purification Methods for Isolating Purified Vesicular Stomatitis Virus from Cell Culture
RU2008140137/10A RU2484135C2 (en) 2006-04-20 2007-04-19 Method of cleaning intended to produce cleaned virus of vesicular stomatitis from cellular culture
EP07755685A EP2007883B1 (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture
AU2007240797A AU2007240797B2 (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture
SI200730829T SI2007883T1 (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture
JP2009506566A JP5129805B2 (en) 2006-04-20 2007-04-19 Purification process for isolation of purified vesicular stomatitis virus from cell culture
KR1020087028205A KR101385927B1 (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture
IL194629A IL194629A (en) 2006-04-20 2008-10-07 Purification processes for isolating purified vesicular stomatitis virus from cell culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79337606P 2006-04-20 2006-04-20
US60/793,376 2006-04-20

Publications (2)

Publication Number Publication Date
WO2007123961A2 WO2007123961A2 (en) 2007-11-01
WO2007123961A3 true WO2007123961A3 (en) 2008-03-06

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PCT/US2007/009510 Ceased WO2007123961A2 (en) 2006-04-20 2007-04-19 Purification processes for isolating purified vesicular stomatitis virus from cell culture

Country Status (19)

Country Link
US (1) US7875446B2 (en)
EP (1) EP2007883B1 (en)
JP (1) JP5129805B2 (en)
KR (1) KR101385927B1 (en)
CN (1) CN101426905B (en)
AT (1) ATE539149T1 (en)
AU (1) AU2007240797B2 (en)
BR (1) BRPI0710820A2 (en)
CA (1) CA2648792C (en)
CR (1) CR10380A (en)
DK (1) DK2007883T3 (en)
ES (1) ES2377356T3 (en)
IL (1) IL194629A (en)
MX (1) MX2008013494A (en)
PL (1) PL2007883T3 (en)
PT (1) PT2007883E (en)
RU (1) RU2484135C2 (en)
SI (1) SI2007883T1 (en)
WO (1) WO2007123961A2 (en)

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