WO2007128441A3 - A novel group of esterases for the production of fine and speciality chemicals - Google Patents

A novel group of esterases for the production of fine and speciality chemicals Download PDF

Info

Publication number
WO2007128441A3
WO2007128441A3 PCT/EP2007/003772 EP2007003772W WO2007128441A3 WO 2007128441 A3 WO2007128441 A3 WO 2007128441A3 EP 2007003772 W EP2007003772 W EP 2007003772W WO 2007128441 A3 WO2007128441 A3 WO 2007128441A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
present
enzyme
nucleotide sequence
encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/003772
Other languages
French (fr)
Other versions
WO2007128441A2 (en
Inventor
Christian Elend
Karl-Erich Jaeger
Christian Leggewie
Christel Schmeisser
Wolfgang Streit
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BRAIN Biotech AG
Original Assignee
BRAIN Biotechnology Research and Information Network AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BRAIN Biotechnology Research and Information Network AG filed Critical BRAIN Biotechnology Research and Information Network AG
Priority to DK07724699.9T priority Critical patent/DK2013336T3/en
Priority to EP07724699.9A priority patent/EP2013336B1/en
Priority to CA2650608A priority patent/CA2650608C/en
Priority to US12/298,874 priority patent/US8956838B2/en
Publication of WO2007128441A2 publication Critical patent/WO2007128441A2/en
Publication of WO2007128441A3 publication Critical patent/WO2007128441A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E. C. 3.1.1.1] activity, wherein the coding sequence is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as depicted in any one of SEQ ID NOs: 2, 4, 6 and 8; (b) a polynucleotide having or comprising a nucleotide sequence encoding an enzyme, wherein the nucleic acid sequence is as shown in any one of SEQ ID NOs: 1, 3, 5 and 7; (c) a polynucleotide having or comprising a nucleotide sequence encoding a fragment or derivative of an enzyme encoded by a polynucleotide of (a) or (b), wherein in said derivative one or more amino acid residues are conservatively substituted compared to the amino acid sequence of (a); (d) a polynucleotide encoding an enzyme having carboxyl esterase activity which polynucleotide is at least 65% identical to a polynucleotide encoding an enzyme as shown in one of SEQ ID NOs: 2, 4, 6 and 8; (e) a polynucleotide having or comprising a nucleotide sequence the complementary strand of which hybridizes to a polynucleotide as defined in any one of (a) to (d); and (T) a polynucleotide having or comprising a nucleotide sequence being degenerate to the nucleotide sequence of the polynucleotide of (d) or (e); or the complementary strand of such a polynucleotide of (a) to (f) or fragments thereof useful as specific probes or primers. The present invention also relates to a host, genetically engineered with the polynucleotide of the present invention or the vector of the present invention. The present invention also relates to a polypeptide comprising the amino acid sequence encoded by a polynucleotide of the present invention or which is obtainable by the process of the present invention. Moreover, the present invention relates to a process for producing said polypeptide and for producing bacteria expressing said polypeptide. Finally, the present invention relates to a composition comprising the polynucleotide of the present invention, the vector of the present invention, the host of the present invention, the polypeptide of the present invention, the antibody of the present invention and/or one or more primers of the present invention.
PCT/EP2007/003772 2006-04-28 2007-04-27 A novel group of esterases for the production of fine and speciality chemicals Ceased WO2007128441A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DK07724699.9T DK2013336T3 (en) 2006-04-28 2007-04-27 New group of esterases for the production of fine and specialty chemicals
EP07724699.9A EP2013336B1 (en) 2006-04-28 2007-04-27 A novel group of esterases for the production of fine and speciality chemicals
CA2650608A CA2650608C (en) 2006-04-28 2007-04-27 A novel group of esterases for the production of fine and speciality chemicals
US12/298,874 US8956838B2 (en) 2006-04-28 2007-04-27 Carboxyl esterase polypeptides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06008953 2006-04-28
EP06008953.9 2006-04-28

Publications (2)

Publication Number Publication Date
WO2007128441A2 WO2007128441A2 (en) 2007-11-15
WO2007128441A3 true WO2007128441A3 (en) 2008-02-28

Family

ID=38421450

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/003772 Ceased WO2007128441A2 (en) 2006-04-28 2007-04-27 A novel group of esterases for the production of fine and speciality chemicals

Country Status (5)

Country Link
US (1) US8956838B2 (en)
EP (1) EP2013336B1 (en)
CA (1) CA2650608C (en)
DK (1) DK2013336T3 (en)
WO (1) WO2007128441A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CO7020177A1 (en) * 2014-04-30 2014-08-11 Corporacion Corpogen Enantioselective modified esterase enzyme and process for obtaining it
CN105779410B (en) * 2014-12-22 2020-08-14 丰益(上海)生物技术研发中心有限公司 Esterase and coding gene thereof
JP2021506252A (en) * 2017-12-13 2021-02-22 コデクシス， インコーポレイテッド Carboxylesterase polypeptide for amide coupling
CN108753805A (en) * 2018-05-29 2018-11-06 安徽师范大学 A kind of heat-resisting esterase encoding gene, carrier, engineering bacteria and its purposes for encoding albumen
CN114540456B (en) * 2020-11-25 2025-04-22 弈柯莱生物科技(集团)股份有限公司 Method for splitting methyl 2-(benzyloxycarbonylamino)-2-(oxetane-3-yl)acetate

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939534A (en) * 1993-12-29 1999-08-17 Sumitomo Pharmaceuticals Company, Limited Factors mutated in the D1 cap region

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ARPIGNY JEAN LOUIS ET AL: "Bacterial lipolytic enzymes: Classification and properties", BIOCHEMICAL JOURNAL, THE BIOCHEMICAL SOCIETY, LONDON, GB, vol. 343, no. 1, 1 October 1999 (1999-10-01), pages 177 - 183, XP002375631, ISSN: 0264-6021 *
DATABASE EMBL [online] 19 May 2006 (2006-05-19), XP002448744, retrieved from EMBL accession no. CP000356 Database accession no. CP000356 *
ELEND C ET AL: "Isolation and biochemical characterization of two novel metagenome-derived esterases", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 5, May 2006 (2006-05-01), pages 3637 - 3645, XP002448481, ISSN: 0099-2240 *
WAGNER ULRIKE G ET AL: "EstB from Burkholderia gladioli: A novel esterase with a beta-lactamase fold reveals steric factors to discriminate between esterolytic and beta-lactam cleaving activity", PROTEIN SCIENCE, vol. 11, no. 3, March 2002 (2002-03-01), pages 467 - 478, XP002448480, ISSN: 0961-8368 *

Also Published As

Publication number Publication date
CA2650608C (en) 2017-02-14
CA2650608A1 (en) 2007-11-15
US8956838B2 (en) 2015-02-17
DK2013336T3 (en) 2016-09-05
EP2013336A2 (en) 2009-01-14
WO2007128441A2 (en) 2007-11-15
US20090221031A1 (en) 2009-09-03
EP2013336B1 (en) 2016-06-22

Similar Documents

Publication Publication Date Title
WO2005080559B1 (en) Fungall cell wall degrading enzyme
WO2008101893A3 (en) Novel sialidase
NZ601188A (en) Woody plants having improved growth characteristics and method for making the same using transcription factors
JP2011224002A5 (en)
RU2002130278A (en) NEW REGULATORY EXPRESS SEQUENCES AND EXPRESS PRODUCTS IN THE FIELD OF GYTHOMYCETES
JP2008526236A5 (en)
WO2003100070A3 (en) The frog prince, a transposon vector for gene transfer in vertebrates
CA2474734A1 (en) Thermostable taq polymerase fragment
WO2008027472A3 (en) Compositions and methods for improved protein production
WO2006076189A3 (en) Maize cyclo1 gene and promoter
WO2007128496A3 (en) A group of esterases for the enantioselective production of fine and speciality chemicals
WO2007128441A3 (en) A novel group of esterases for the production of fine and speciality chemicals
NZ601950A (en) Method for producing pyripyropene
UA97642C2 (en) Genetic markers for high oleic acid content in plants
Zackova Suchanova et al. Diatom adhesive trail proteins acquired by horizontal gene transfer from bacteria serve as primers for marine biofilm formation
JP5392942B2 (en) Novel alkaline alginate lyase and its utilization
WO2009143155A3 (en) Delayed fruit deterioration allele in plants and methods of detection
WO2012154024A1 (en) Cis-prenyl transferase from the plant hevea brasiliensis
WO2014028773A3 (en) Lignin degrading enzymes from macrophomina phaseolina and uses thereof
CN104232604B (en) Polygalacturonase PG7fn and gene and application thereof
AU2001273977A1 (en) Novel nucleotide sequences coding for the glbo gene
WO2006019095A1 (en) Novel urethanase gene
CN106566837A (en) Iota-carrageenan enzyme gene derived from marine bacteria and applications of iota-carrageenan enzyme gene
EP3550014B1 (en) Directed evolution of cyp52a12 gene and its use in dicarboxylic acid production
WO2006095769A1 (en) Microorganism-derived psychrophilic endonuclease

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07724699

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2650608

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2007724699

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 12298874

Country of ref document: US