WO2007133496A3 - Development of a novel assay for mgmt (methyl guanine transferase) - Google Patents

Development of a novel assay for mgmt (methyl guanine transferase) Download PDF

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Publication number
WO2007133496A3
WO2007133496A3 PCT/US2007/010998 US2007010998W WO2007133496A3 WO 2007133496 A3 WO2007133496 A3 WO 2007133496A3 US 2007010998 W US2007010998 W US 2007010998W WO 2007133496 A3 WO2007133496 A3 WO 2007133496A3
Authority
WO
WIPO (PCT)
Prior art keywords
mgmt
alkylating agents
present
technique
development
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/010998
Other languages
French (fr)
Other versions
WO2007133496A2 (en
Inventor
Bimalendu Dasmahapatra
Mark Demma
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme LLC
Original Assignee
Schering Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering Corp filed Critical Schering Corp
Priority to EP07776830A priority Critical patent/EP2018558A2/en
Publication of WO2007133496A2 publication Critical patent/WO2007133496A2/en
Publication of WO2007133496A3 publication Critical patent/WO2007133496A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91005—Transferases (2.) transferring one-carbon groups (2.1)
    • G01N2333/91011—Methyltransferases (general) (2.1.1.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides improved methods for assessing the level of MGMT activity in a variety of biological preparations. MGMT, a DNA repair enzyme, can reduce the chemotherapeutic efficacy of alkylating agents by repairing the damage that alkylating agents do to tumor cell DNA. The methods of the present invention can be used, inter alia, to measure MGMT levels and to thereby predict the clinical response to alkylating agents. The present invention includes three preferred assays for assessment of MGMT activity: (1 ) the immunoassay technique, (2) the labeled O6-BG technique, and (3) the fluorescence polarization technique. Kits useful for the performance of such assays are also provided.
PCT/US2007/010998 2006-05-09 2007-05-07 Development of a novel assay for mgmt (methyl guanine transferase) Ceased WO2007133496A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07776830A EP2018558A2 (en) 2006-05-09 2007-05-07 Development of a novel assay for mgmt (methyl guanine transferase)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79891406P 2006-05-09 2006-05-09
US60/798,914 2006-05-09

Publications (2)

Publication Number Publication Date
WO2007133496A2 WO2007133496A2 (en) 2007-11-22
WO2007133496A3 true WO2007133496A3 (en) 2008-07-24

Family

ID=38646602

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/010998 Ceased WO2007133496A2 (en) 2006-05-09 2007-05-07 Development of a novel assay for mgmt (methyl guanine transferase)

Country Status (3)

Country Link
US (1) US20070264672A1 (en)
EP (1) EP2018558A2 (en)
WO (1) WO2007133496A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070264667A1 (en) * 2003-09-03 2007-11-15 Robert Gellibolian Method and system for assaying transferase activity
CN102443018B (en) * 2011-10-06 2014-09-10 浙江大学 Fluorescence-labeled O6-benzylguanine and its preparation and application
CN102707060B (en) * 2012-05-31 2015-05-20 杭州师范大学 Chemiluminescent test kit for testing activity of MGMT (O6-Methylguanine DNA-Methyltransferase) and test method
CN102798717B (en) * 2012-06-15 2014-11-26 杭州师范大学 A kind of detection method of O6-methylguanine-DNA methyltransferase activity
CN103713119B (en) * 2013-12-31 2015-06-17 上海科华生物工程股份有限公司 Kit for detecting performance of full-automatic chemiluminescence immunoassay instrument
CN103901020B (en) * 2014-03-31 2015-10-21 西北大学 Method and application of detecting DNA methyltransferase with electrochemiluminescence biosensor
CN117030998A (en) * 2023-08-24 2023-11-10 南京医科大学 A method for quantitative detection of O6-methylguanine levels

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993024837A1 (en) * 1992-05-22 1993-12-09 Applied Genetics Inc. Assays for o6-methylguanine-dna methyltransferase

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US5731304A (en) * 1982-08-23 1998-03-24 Cancer Research Campaign Technology Potentiation of temozolomide in human tumour cells
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6017706A (en) * 1997-05-07 2000-01-25 The United States Of America As Represented By The Department Of Health And Human Services Process for identifying compounds which protect against the formation of fluorescent light induced DNA lesions and x-ray-induced lesions
CA2358683A1 (en) * 1999-02-02 2000-08-10 Caliper Technologies Corporation Methods, devices and systems for characterizing proteins
US6773897B2 (en) * 2000-09-29 2004-08-10 The Johns Hopkins University School Of Medicine Method of predicting the clinical response to chemotherapeutic treatment with alkylating agents
NZ554831A (en) * 2004-11-09 2009-10-30 Schering Corp Improved dosing regimen of temozolomide for treating cancer based on the patientÆs MGMT level

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993024837A1 (en) * 1992-05-22 1993-12-09 Applied Genetics Inc. Assays for o6-methylguanine-dna methyltransferase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DOLAN M E ET AL: "DEPLETION OF MAMMALIAN O-6 ALKYLGUANINE DNA ALKYLTRANSFERASE ACTIVITY BY O-6 BENZYLGUANINE PROVIDES A MEANS TO EVALUATE THE ROLE OF THIS PROTEIN IN PROTECTION AGAINST CARCINOGENIC THERAPEUTIC ALKYLATING AGENTS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 87, no. 14, 1990, pages 5368 - 5372, XP002459184, ISSN: 0027-8424 *
NAGEL G ET AL: "DNA repair protein O<6>-methylguanine-DNA methyltransferase in testis and testicular tumors as determined by a novel nonradioactive assay", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 321, no. 1, 1 October 2003 (2003-10-01), pages 38 - 43, XP004453607, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
EP2018558A2 (en) 2009-01-28
WO2007133496A2 (en) 2007-11-22
US20070264672A1 (en) 2007-11-15

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