WO2007138049A3 - Chloramphenicol resistance selection in bacillus licheniformis - Google Patents

Chloramphenicol resistance selection in bacillus licheniformis Download PDF

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Publication number
WO2007138049A3
WO2007138049A3 PCT/EP2007/055190 EP2007055190W WO2007138049A3 WO 2007138049 A3 WO2007138049 A3 WO 2007138049A3 EP 2007055190 W EP2007055190 W EP 2007055190W WO 2007138049 A3 WO2007138049 A3 WO 2007138049A3
Authority
WO
WIPO (PCT)
Prior art keywords
bacillus licheniformis
chloramphenicol resistance
resistance selection
chloramphenicol
cat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/055190
Other languages
French (fr)
Other versions
WO2007138049A2 (en
Inventor
Steen Troels Joergensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to CN200780027873XA priority Critical patent/CN101495625B/en
Priority to US12/302,047 priority patent/US8431382B2/en
Priority to EP07729612A priority patent/EP2029732B1/en
Priority to DE602007002568T priority patent/DE602007002568D1/en
Priority to DK07729612.7T priority patent/DK2029732T3/en
Priority to AT07729612T priority patent/ATE443760T1/en
Publication of WO2007138049A2 publication Critical patent/WO2007138049A2/en
Publication of WO2007138049A3 publication Critical patent/WO2007138049A3/en
Anticipated expiration legal-status Critical
Priority to US13/855,090 priority patent/US8993304B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a modified Bacillus licheniformis host cell, wherein one or more naturally occurring chromosomal chloramphenicol acetyl transferase encoding gene(s), cat, has been inactivated. The inactivation of the chromosomal cat gene(s) allows the use of chloramphenicol as an efficient selective agent in methods for DNA introduction into the modified cell.
PCT/EP2007/055190 2006-05-31 2007-05-29 Chloramphenicol resistance selection in bacillus licheniformis Ceased WO2007138049A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN200780027873XA CN101495625B (en) 2006-05-31 2007-05-29 Chloramphenicol resistance selection in bacillus licheniformis
US12/302,047 US8431382B2 (en) 2006-05-31 2007-05-29 Chloramphenicol resistance selection in Bacillus licheniformis
EP07729612A EP2029732B1 (en) 2006-05-31 2007-05-29 Chloramphenicol resistance selection in bacillus licheniformis
DE602007002568T DE602007002568D1 (en) 2006-05-31 2007-05-29 CHLORAMPHENICOL RESISTANCE SELECTION AT BACILLUS LICHENIFORMIS
DK07729612.7T DK2029732T3 (en) 2006-05-31 2007-05-29 Chloramphenicol resistance selection in Bacillus licheniformis
AT07729612T ATE443760T1 (en) 2006-05-31 2007-05-29 CHLORAMPHENICOL RESISTANCE SELECTION IN BACILLUS licheniformis
US13/855,090 US8993304B2 (en) 2006-05-31 2013-04-02 Chloramphenicol resistance selection in Bacillus licheniformis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200600742 2006-05-31
DKPA200600742 2006-05-31

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/302,047 A-371-Of-International US8431382B2 (en) 2006-05-31 2007-05-29 Chloramphenicol resistance selection in Bacillus licheniformis
US13/855,090 Continuation US8993304B2 (en) 2006-05-31 2013-04-02 Chloramphenicol resistance selection in Bacillus licheniformis

Publications (2)

Publication Number Publication Date
WO2007138049A2 WO2007138049A2 (en) 2007-12-06
WO2007138049A3 true WO2007138049A3 (en) 2008-01-31

Family

ID=38529453

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/055190 Ceased WO2007138049A2 (en) 2006-05-31 2007-05-29 Chloramphenicol resistance selection in bacillus licheniformis

Country Status (7)

Country Link
US (2) US8431382B2 (en)
EP (1) EP2029732B1 (en)
CN (1) CN101495625B (en)
AT (1) ATE443760T1 (en)
DE (1) DE602007002568D1 (en)
DK (1) DK2029732T3 (en)
WO (1) WO2007138049A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007138049A2 (en) * 2006-05-31 2007-12-06 Novozymes A/S Chloramphenicol resistance selection in bacillus licheniformis
US11130969B2 (en) 2013-07-12 2021-09-28 Novozymes, A/S Direct transfer of polynucleotides between genomes
EP3481959A1 (en) 2016-07-06 2019-05-15 Novozymes A/S Improving a microorganism by crispr-inhibition
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis
WO2018134386A1 (en) 2017-01-23 2018-07-26 Novozymes A/S Host cells and methods for producing double-stranded rna
DK3641550T3 (en) 2017-06-22 2021-07-05 Novozymes As PROCEDURE FOR IMPROVING STRETCHABILITY OF DOUGH USING GAMMA-GLUTAMYL TRANSPPTIDASE
EP3707252A1 (en) 2017-11-10 2020-09-16 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
CN108913630A (en) * 2018-08-01 2018-11-30 广东容大生物股份有限公司 A kind of slant medium of bacillus licheniformis
FR3090691B1 (en) * 2018-12-20 2023-06-09 Ifp Energies Now GENETICALLY MODIFIED CLOSTRIDIUM BACTERIA, PREPARATION AND USES THEREOF
CN113939588A (en) 2019-05-15 2022-01-14 诺维信公司 Temperature sensitive RNA guided endonucleases
CN114207125B (en) 2019-06-25 2025-01-28 诺维信公司 Counterselection by repression of conditionally essential genes
EP4370534A1 (en) 2021-07-13 2024-05-22 Novozymes A/S Recombinant cutinase expression
WO2025132815A1 (en) 2023-12-20 2025-06-26 Novozymes A/S Novel cas nucleases and polynucleotides encoding the same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003083125A1 (en) * 2002-03-29 2003-10-09 Genencor International, Inc. Ehanced protein expression in bacillus

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1177380A (en) * 1995-01-23 1998-03-25 诺沃挪第克公司 DNA integration by transposition
US20020068352A1 (en) * 2000-05-12 2002-06-06 Novozymes A/S Alpha-amylase variants with altered 1, 6-activity
CN1318575C (en) * 2003-09-10 2007-05-30 中国人民解放军军事医学科学院生物工程研究所 Protein conformational disease cell model and its use
JP2007532117A (en) 2004-04-08 2007-11-15 ジェネンコー・インターナショナル・インク α-amylase mutant
WO2007138049A2 (en) * 2006-05-31 2007-12-06 Novozymes A/S Chloramphenicol resistance selection in bacillus licheniformis
BRPI0808513A2 (en) * 2007-03-09 2014-08-19 Danisco Us Inc Genencor Div ALPHA-AMILASE VARIANTS OF ALKALIFYL BACILLUS SPECIES, COMPOSITIONS UNDERSTANDING ALPHA-AMYLASE VARIANTS AND METHODS OF USE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003083125A1 (en) * 2002-03-29 2003-10-09 Genencor International, Inc. Ehanced protein expression in bacillus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VEITH B ET AL: "THE COMPLETE GENOME SEQUENCE OF BACILLUS LICHENIFORMIS DSM13, AN ORGANISM WITH GREAT INDUSTRIAL POTENTIAL", JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY, HORIZON SCIENTIFIC PRESS, WYMONDHAM,, GB, vol. 7, no. 4, 2004, pages 204 - 211, XP009047713, ISSN: 1464-1801 *

Also Published As

Publication number Publication date
EP2029732A2 (en) 2009-03-04
DE602007002568D1 (en) 2009-11-05
ATE443760T1 (en) 2009-10-15
CN101495625A (en) 2009-07-29
EP2029732B1 (en) 2009-09-23
US20090280569A1 (en) 2009-11-12
CN101495625B (en) 2012-11-14
WO2007138049A2 (en) 2007-12-06
DK2029732T3 (en) 2010-02-01
US8993304B2 (en) 2015-03-31
US8431382B2 (en) 2013-04-30
US20130210152A1 (en) 2013-08-15

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