WO2007149791A3 - System for isolating biomolecules from a sample - Google Patents

System for isolating biomolecules from a sample Download PDF

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Publication number
WO2007149791A3
WO2007149791A3 PCT/US2007/071402 US2007071402W WO2007149791A3 WO 2007149791 A3 WO2007149791 A3 WO 2007149791A3 US 2007071402 W US2007071402 W US 2007071402W WO 2007149791 A3 WO2007149791 A3 WO 2007149791A3
Authority
WO
WIPO (PCT)
Prior art keywords
cartridge
fluids
purification
sample
isolating biomolecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/071402
Other languages
French (fr)
Other versions
WO2007149791A2 (en
Inventor
Jeffrey C Braman
Lee Scott Basehore
Natalia Novoradovskaya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stratagene California
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California filed Critical Stratagene California
Priority to EP07812176A priority Critical patent/EP2035540A2/en
Priority to JP2009530708A priority patent/JP2009540868A/en
Publication of WO2007149791A2 publication Critical patent/WO2007149791A2/en
Publication of WO2007149791A3 publication Critical patent/WO2007149791A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The present invention provides an automated system for purification of a substance of interest. The system generally comprises an instrument for moving fluids through the system, a reagent pack for storing fluids, and a purification cartridge. The cartridge comprises two filtration units for binding substances based on different physical properties. The cartridge also comprises rotary valves for control of movement of fluids on the cartridge. In preferred embodiments, the system is useful for purifying RNA from blood samples.
PCT/US2007/071402 2006-06-15 2007-06-15 System for isolating biomolecules from a sample Ceased WO2007149791A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07812176A EP2035540A2 (en) 2006-06-15 2007-06-15 System for isolating biomolecules from a sample
JP2009530708A JP2009540868A (en) 2006-06-15 2007-06-15 System for isolating biomolecules from samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81462206P 2006-06-15 2006-06-15
US60/814,622 2006-06-15

Publications (2)

Publication Number Publication Date
WO2007149791A2 WO2007149791A2 (en) 2007-12-27
WO2007149791A3 true WO2007149791A3 (en) 2008-11-27

Family

ID=38834277

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/071402 Ceased WO2007149791A2 (en) 2006-06-15 2007-06-15 System for isolating biomolecules from a sample

Country Status (4)

Country Link
US (1) US20080026451A1 (en)
EP (1) EP2035540A2 (en)
JP (1) JP2009540868A (en)
WO (1) WO2007149791A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104833626A (en) * 2014-05-16 2015-08-12 麦君宇 Method and apparatus for biomolecular analysis

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Publication number Priority date Publication date Assignee Title
CN104833626A (en) * 2014-05-16 2015-08-12 麦君宇 Method and apparatus for biomolecular analysis

Also Published As

Publication number Publication date
US20080026451A1 (en) 2008-01-31
JP2009540868A (en) 2009-11-26
EP2035540A2 (en) 2009-03-18
WO2007149791A2 (en) 2007-12-27

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