WO2008012785A3 - A probe for cellular oxygen - Google Patents

A probe for cellular oxygen Download PDF

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Publication number
WO2008012785A3
WO2008012785A3 PCT/IE2007/000071 IE2007000071W WO2008012785A3 WO 2008012785 A3 WO2008012785 A3 WO 2008012785A3 IE 2007000071 W IE2007000071 W IE 2007000071W WO 2008012785 A3 WO2008012785 A3 WO 2008012785A3
Authority
WO
WIPO (PCT)
Prior art keywords
probe
oxygen
cellular oxygen
sensing
cellular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IE2007/000071
Other languages
French (fr)
Other versions
WO2008012785A2 (en
Inventor
Dimitri Papkovsky
Tomas O'riordan
Gelii V Ponomarev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University College Cork
Original Assignee
University College Cork
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University College Cork filed Critical University College Cork
Priority to EP07766783A priority Critical patent/EP2043694A2/en
Publication of WO2008012785A2 publication Critical patent/WO2008012785A2/en
Publication of WO2008012785A3 publication Critical patent/WO2008012785A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0015—Phosphorescence
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0036—Porphyrins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/005—Fluorescence in vivo characterised by the carrier molecule carrying the fluorescent agent
    • A61K49/0056—Peptides, proteins, polyamino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Sustainable Development (AREA)
  • Optics & Photonics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

A probe for sensing and imaging intracellular oxygen comprises an oxygen-sensitive fluorescent or phosphorescent dye combined with a hydrophilic macromolecular carrier and a cell loading agent. A method for sensing cellular oxygen using the probe is also described.
PCT/IE2007/000071 2006-07-24 2007-07-24 A probe for cellular oxygen Ceased WO2008012785A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07766783A EP2043694A2 (en) 2006-07-24 2007-07-24 A probe for cellular oxygen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IE20060544 2006-07-24
IE2006/0544 2006-07-24

Publications (2)

Publication Number Publication Date
WO2008012785A2 WO2008012785A2 (en) 2008-01-31
WO2008012785A3 true WO2008012785A3 (en) 2008-03-27

Family

ID=38828576

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IE2007/000071 Ceased WO2008012785A2 (en) 2006-07-24 2007-07-24 A probe for cellular oxygen

Country Status (3)

Country Link
US (1) US20080051646A1 (en)
EP (1) EP2043694A2 (en)
WO (1) WO2008012785A2 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4358785B2 (en) * 2005-05-27 2009-11-04 麒麟麦酒株式会社 Method for measuring oxygen content in sealed container and piercing device for sealed container used therefor
US9874520B1 (en) 2008-11-07 2018-01-23 Mocon, Inc. Epi-fluoresence confocal optical analyte sensor
US8241911B2 (en) * 2008-11-07 2012-08-14 Mocon, Inc. Calibration card for photoluminescent oxygen sensors with zero point maintained with a metal-air battery
CN102265136B (en) * 2008-11-07 2014-08-13 膜康公司 Calibration card for oxygen optical sensors
US8323978B2 (en) * 2008-11-07 2012-12-04 Mocon, Inc. Calibration system and technique for photoluminescent oxygen sensors with zero point maintained with a metal-air battery
US8834795B2 (en) 2008-12-11 2014-09-16 Luxcel Biosciences Limited Optochemical sensor for sensing O2, and method of its preparation
WO2010143172A1 (en) * 2009-06-08 2010-12-16 University College Cork, National University Of Ireland Cork Conjugates of cell- penetrating peptides and phosphorescent metalloporphyrins for intracellular oxygen measurement
US20110136247A1 (en) 2009-12-07 2011-06-09 Dmitri Boris Papkovsky Photoluminescent oxygen probe with reduced cross-sensitivity to humidity
US9188536B2 (en) 2010-01-27 2015-11-17 Luxcel Biosciences, Ltd Photoluminescent pressure probe
JP2011185842A (en) * 2010-03-10 2011-09-22 Fujifilm Corp Method and device for analyzing low oxygen region of biosample by time resolving measurement of light induced self-fluorescence
EP2371925B1 (en) * 2010-03-29 2012-12-12 University College Cork, National University of Ireland, Cork A sensor material, and uses thereof to simultaneously sense two analytes
US20120129268A1 (en) 2010-11-19 2012-05-24 Mayer Daniel W Photoluminescent oxygen probe with reduced cross-sensitivity to humidity
US9274060B1 (en) * 2011-01-13 2016-03-01 Mocon, Inc. Methods for transmembrane measurement of oxygen concentration and monitoring changes in oxygen concentration within a space enclosed by a membrane employing a photoluminescent transmembrane oxygen probe
US9121827B2 (en) 2011-06-30 2015-09-01 Mocon, Inc. Method of contemporaneously monitoring changes in analyte concentration in a plurality of samples on individual schedules
WO2013023697A1 (en) 2011-08-17 2013-02-21 Luxcel Biosciences Limited Tool and method for validating operational performance of a photoluminesence based analytical instrument
EP2786128A1 (en) 2011-09-06 2014-10-08 Luxcel Biosciences Limited Dry laminated photoluminescent probe and methods of manufacture and use
US20140329332A1 (en) 2011-11-22 2014-11-06 Luxcel Biosciences Ltd. Device and method for rapid assay of multiple biological samples for oxygen consumption
CN102633932B (en) * 2012-03-16 2014-06-04 华东理工大学 Characteristic fluorophore contained metal complex and application thereof
US11293866B2 (en) 2012-03-22 2022-04-05 John EASTMAN Fiber optic analyte sensor
US9057687B2 (en) 2012-04-20 2015-06-16 Mocon, Inc. Calibration vial and technique for calibrating a fiber optic oxygen sensing needle
US8658429B1 (en) 2012-08-06 2014-02-25 Mocon, Inc. Photoluminescent oxygen probe tack
WO2014086411A1 (en) 2012-12-05 2014-06-12 Luxcel Biosciences Limited Individually and flexibly deployable target-analyte sensitive particulate probes and method of making and using
WO2014153428A1 (en) * 2013-03-19 2014-09-25 Surgisense Corporation Apparatus, systems and methods for determining tissue oxygenation
US20150282749A1 (en) 2014-04-05 2015-10-08 Surgisense Corporation Apparatus, systems, and methods for mapping of tissue oxygenation
US9316554B1 (en) 2014-12-23 2016-04-19 Mocon, Inc. Fiber optic analyte sensor with integrated in situ total pressure correction
CN111326059B (en) * 2020-02-22 2021-08-17 复旦大学 An encryption and anti-counterfeiting method based on fluorescence or phosphorescence stimulus response
US11467151B1 (en) 2021-12-23 2022-10-11 United Arab Emirates University Phosphorescence oxygen analyzer and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998026708A1 (en) * 1996-12-16 1998-06-25 The Trustees Of The University Of Pennsylvania Phosphorescent dendritic macromolecular compounds for imaging tissue oxygen

Family Cites Families (11)

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DE2508637C3 (en) * 1975-02-28 1979-11-22 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V., 3400 Goettingen Arrangement for the optical measurement of blood gases
US5030420A (en) * 1982-12-23 1991-07-09 University Of Virginia Alumni Patents Foundation Apparatus for oxygen determination
US4810655A (en) * 1985-07-03 1989-03-07 Abbott Laboratories Method for measuring oxygen concentration
US5232839A (en) * 1990-06-14 1993-08-03 Difco Laboratories Incorporated Detecting microbiological growth
US5718842A (en) * 1994-10-07 1998-02-17 Joanneum Reserach Forschungsgesellschaft Mbh Luminescent dye comprising metallocomplex of a oxoporphyrin
FI982422A0 (en) * 1998-11-09 1998-11-09 Arctic Diagnostics Oy Porphyrin compounds, their conjugates and assay methods based on the use of said conjugate
US6395555B1 (en) * 1999-10-14 2002-05-28 David F. Wilson Method and apparatus for determining the effect of a drug on cells
US7789039B2 (en) * 2001-06-22 2010-09-07 Université Catholique de Louvain Hydrogel beads or capsules as artificial media for insects oviposition and rearing of endoparasitoids
ATE500894T1 (en) * 2002-01-17 2011-03-15 Univ College Cork Nat Univ Ie TEST DEVICE AND METHOD FOR CHEMICAL OR BIOLOGICAL SCREENING
CA2541385C (en) * 2003-09-05 2013-11-19 The Ohio State University Research Foundation Nanoparticulate probe for in vivo monitoring of tissue oxygenation
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Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO1998026708A1 (en) * 1996-12-16 1998-06-25 The Trustees Of The University Of Pennsylvania Phosphorescent dendritic macromolecular compounds for imaging tissue oxygen

Non-Patent Citations (7)

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Title
HOGAN M C: "Phosphorescence quenching method for measurement of intracellular PO2 in isolated skeletal muscle fibers.", JOURNAL OF APPLIED PHYSIOLOGY (BETHESDA, MD. : 1985) FEB 1999, vol. 86, no. 2, February 1999 (1999-02-01), pages 720 - 724, XP002465643, ISSN: 8750-7587 *
JI J ET AL: "Molecular oxygen-sensitive fluorescent lipobeads for intracellular oxygen measurements in murine macrophages.", ANALYTICAL CHEMISTRY 1 AUG 2001, vol. 73, no. 15, 1 August 2001 (2001-08-01), pages 3521 - 3527, XP002465648, ISSN: 0003-2700 *
JOHANNES TANJA ET AL: "Dual-wavelength phosphorimetry for determination of cortical and subcortical microvascular oxygenation in rat kidney.", JOURNAL OF APPLIED PHYSIOLOGY (BETHESDA, MD. : 1985) APR 2006, vol. 100, no. 4, April 2006 (2006-04-01), pages 1301 - 1310, XP002465646, ISSN: 8750-7587 *
MARTSEV S P ET AL: "Modification of monoclonal and polyclonal IgG with palladium (II) coproporphyrin I: stimulatory and inhibitory functional effects induced by two different methods", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 186, no. 2, 16 October 1995 (1995-10-16), pages 293 - 304, XP004021230, ISSN: 0022-1759 *
O'RIORDAN TOMÁS C ET AL: "Sensing intracellular oxygen using near-infrared phosphorescent probes and live-cell fluorescence imaging.", AMERICAN JOURNAL OF PHYSIOLOGY. REGULATORY, INTEGRATIVE AND COMPARATIVE PHYSIOLOGY APR 2007, vol. 292, no. 4, April 2007 (2007-04-01), pages R1613 - R1620, XP008087651, ISSN: 0363-6119 *
TAKAHASHI EIJI ET AL: "In vivo oxygen imaging using green fluorescent protein.", AMERICAN JOURNAL OF PHYSIOLOGY. CELL PHYSIOLOGY OCT 2006, vol. 291, no. 4, 31 May 2006 (2006-05-31), pages C781 - C787, XP002465644, ISSN: 0363-6143 *
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Also Published As

Publication number Publication date
US20080051646A1 (en) 2008-02-28
WO2008012785A2 (en) 2008-01-31
EP2043694A2 (en) 2009-04-08

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