WO2008025662A3 - Verfahren zur quantitativen bestimmung der kolokalisation von molekülmarkern in gewebeschnitten - Google Patents

Verfahren zur quantitativen bestimmung der kolokalisation von molekülmarkern in gewebeschnitten Download PDF

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Publication number
WO2008025662A3
WO2008025662A3 PCT/EP2007/058280 EP2007058280W WO2008025662A3 WO 2008025662 A3 WO2008025662 A3 WO 2008025662A3 EP 2007058280 W EP2007058280 W EP 2007058280W WO 2008025662 A3 WO2008025662 A3 WO 2008025662A3
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WO
WIPO (PCT)
Prior art keywords
molecular markers
tissue
colocalization
quantitative determination
sections
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/058280
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English (en)
French (fr)
Other versions
WO2008025662A2 (de
Inventor
Ansgar Pommer
Bernd Bonnekoh
Raik Boeckelmann
Harald Gollnik
Lars Phillipsen
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SKINSYSTEC GmbH
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SKINSYSTEC GmbH
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Publication date
Application filed by SKINSYSTEC GmbH filed Critical SKINSYSTEC GmbH
Priority to EP07788341A priority Critical patent/EP2059906A2/de
Publication of WO2008025662A2 publication Critical patent/WO2008025662A2/de
Publication of WO2008025662A3 publication Critical patent/WO2008025662A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Processing (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur quantitativen Bestimmung der Kolokalisation von wenigstens zwei Molekülmarkern in Gewebeschnitten, insbesondere in Haut- und Schleimhautgewebeschnitten. Das erfindungsgemäße Verfahren ist durch folgende Verfahrensschritte gekennzeichnet: Erzeugung eines digitalisierten Bildes des Gewebeschnittes für jeden der wenigstens zwei Molekülmarker zur Darstellung des jeweiligen Molekülmarkers, Überlagerung der digitalisierten Bilder zu einem Summenbild, bildpunktweise Quantisierung des Molekülmarkersignals für jeden der wenigstens zwei Molekülmarker in dem Summenbild, Erzeugung einer Matrix, wobei jedem Bildpunkt ein Vektor zugeordnet wird, dessen Komponenten die quantisierten Molekülmarkersignale der wenigstens zwei Molekülmarker sind und gewebespezifische standardisierte Ausrichtung der Matrix. Ferner betrifft die Erfindung eine Vorrichtung zur quantitativen Bestimmung der Kolokalisation von wenigstens zwei Molekülmarkern in Gewebeschnitten, insbesondere in Haut- und Schleimhautgewebeschnitten sowie eine Verwendung des erfindungsgemäßen Verfahrens.
PCT/EP2007/058280 2006-08-30 2007-08-09 Verfahren zur quantitativen bestimmung der kolokalisation von molekülmarkern in gewebeschnitten Ceased WO2008025662A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07788341A EP2059906A2 (de) 2006-08-30 2007-08-09 Verfahren zur quantitativen bestimmung der kolokalisation von molekülmarkern in gewebeschnitten

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200610040513 DE102006040513A1 (de) 2006-08-30 2006-08-30 Verfahren zur quantitativen Bestimmung der Kolokalisation von Molekülmarkern in Gewebeschnitten
DE102006040513.7 2006-08-30

Publications (2)

Publication Number Publication Date
WO2008025662A2 WO2008025662A2 (de) 2008-03-06
WO2008025662A3 true WO2008025662A3 (de) 2008-07-24

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PCT/EP2007/058280 Ceased WO2008025662A2 (de) 2006-08-30 2007-08-09 Verfahren zur quantitativen bestimmung der kolokalisation von molekülmarkern in gewebeschnitten

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Country Link
EP (1) EP2059906A2 (de)
DE (1) DE102006040513A1 (de)
WO (1) WO2008025662A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102369530B (zh) 2009-05-28 2016-05-18 皇家飞利浦电子股份有限公司 用于副作用预后和监测的装置
CN104165873A (zh) * 2014-07-22 2014-11-26 中国科学院植物研究所 一种检测活体植物细胞中两种膜蛋白共定位程度的方法

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DE19709348C2 (de) * 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
US6294331B1 (en) * 1997-08-08 2001-09-25 The United States Of America As Represented By The Department Of Health And Human Services Methods for assessing genetic and phenotypic markers by simultaneous multicolor visualization of chromogenic dyes using brightfield microscopy and spectral imaging
CA2350692A1 (en) * 1998-10-29 2000-05-11 Cell Works Diagnostics, Inc. Multiple marker characterization of single cells
US6678398B2 (en) * 2000-09-18 2004-01-13 Sti Medical Systems, Inc. Dual mode real-time screening and rapid full-area, selective-spectral, remote imaging and analysis device and process
WO2002079752A2 (en) * 2001-03-30 2002-10-10 Molecular Diagnostics, Inc Detection of abnormal cells
WO2006075333A2 (en) * 2005-01-13 2006-07-20 Spectrum Dynamics Llc Multi-dimensional image reconstruction and analysis for expert-system diagnosis

Non-Patent Citations (4)

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Title
BONNEKOH B ET AL: "THE CD11A BINDING SITE OF EFALIZUMAB IN PSORIATIC SKIN TISSUE AS ANALYZED BY MULTI-EPITOPE LIGAND CARTOGRAPHY ROBOT TECHNOLOGY. INTRODUCTION OF A NOVEL BIOLOGICAL DRUG-BINDING BIOCHIP ASSAY", SKIN PHARMACOLOGY AND SKIN APPLIED PHYSIOLOGY, KARGER, BASEL,, CH, vol. 20, no. 2, 13 December 2006 (2006-12-13), pages 96 - 111, XP008079834, ISSN: 1422-2868 *
SCHUBERT W: "MOLEKULARE NETZWERKE HERAUSFORDERUNG FUER BIOLOGIE UND PHARMAFORSCHUNG", MAGDEBURGER WISSENSCHAFTSJOURNAL, 2004, XP002442971, Retrieved from the Internet <URL:http://www.uni-magdeburg.de/MWJ/MWJ2004/schubert.pdf> [retrieved on 20070718] *
SCHUBERT WALTER: "Exploring molecular networks directly in the cell", CYTOMETRY. PART A, JOHN WILEY, HOBOKEN, NJ, US, vol. 69, no. 3, March 2006 (2006-03-01), pages 109 - 112, XP002442970, ISSN: 1552-4922 *
SCHUBERT WALTER: "TOPOLOGICAL PROTEOMICS, TOPONOMICS, MELK-TECHNOLOGY", ADVANCES IN BIOCHEMICAL ENGINEERING, BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 83, 2003, pages 189 - 209, XP008079848, ISSN: 0724-6145 *

Also Published As

Publication number Publication date
DE102006040513A1 (de) 2008-03-27
EP2059906A2 (de) 2009-05-20
WO2008025662A2 (de) 2008-03-06

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