WO2008028115A3 - Methods for producing transgenic plants - Google Patents

Methods for producing transgenic plants Download PDF

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Publication number
WO2008028115A3
WO2008028115A3 PCT/US2007/077361 US2007077361W WO2008028115A3 WO 2008028115 A3 WO2008028115 A3 WO 2008028115A3 US 2007077361 W US2007077361 W US 2007077361W WO 2008028115 A3 WO2008028115 A3 WO 2008028115A3
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WO
WIPO (PCT)
Prior art keywords
methods
transgenic plants
producing transgenic
producing
ergonomic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/077361
Other languages
French (fr)
Other versions
WO2008028115A2 (en
Inventor
Anisha Akula
David R Duncan
Brenda Lowe
Michael T Mann
William L Petersen
Jyoti R Rout
David D Songstad
Joel B Wilks
Zhang Wanggen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Technology LLC
Original Assignee
Monsanto Technology LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to BRPI0716373-8A priority Critical patent/BRPI0716373B1/en
Priority to DE602007012269T priority patent/DE602007012269D1/en
Priority to AU2007289105A priority patent/AU2007289105B2/en
Priority to AT07814616T priority patent/ATE497008T1/en
Priority to EP07814616A priority patent/EP2057272B1/en
Priority to CA2666821A priority patent/CA2666821C/en
Priority to MX2009002248A priority patent/MX2009002248A/en
Priority to CN2007800400731A priority patent/CN101528934B/en
Application filed by Monsanto Technology LLC filed Critical Monsanto Technology LLC
Priority to BR122017012510-9A priority patent/BR122017012510B1/en
Priority to JP2009526926A priority patent/JP5350246B2/en
Publication of WO2008028115A2 publication Critical patent/WO2008028115A2/en
Publication of WO2008028115A3 publication Critical patent/WO2008028115A3/en
Priority to ZA2009/01140A priority patent/ZA200901140B/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265—Transgene containment, e.g. gene dispersal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Inorganic Compounds Of Heavy Metals (AREA)

Abstract

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
PCT/US2007/077361 2006-08-31 2007-08-31 Methods for producing transgenic plants Ceased WO2008028115A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
MX2009002248A MX2009002248A (en) 2006-08-31 2007-08-31 Methods for producing transgenic plants.
AU2007289105A AU2007289105B2 (en) 2006-08-31 2007-08-31 Methods for producing transgenic plants
AT07814616T ATE497008T1 (en) 2006-08-31 2007-08-31 METHOD FOR PRODUCING TRANSGENIC PLANTS
EP07814616A EP2057272B1 (en) 2006-08-31 2007-08-31 Methods for producing transgenic plants
CA2666821A CA2666821C (en) 2006-08-31 2007-08-31 Method for producing transgenic corn plants in a single container
CN2007800400731A CN101528934B (en) 2006-08-31 2007-08-31 Method for producing transgenic plants
BR122017012510-9A BR122017012510B1 (en) 2006-08-31 2007-08-31 Processes of production of a culture of transformable and embryogenic maize cells
BRPI0716373-8A BRPI0716373B1 (en) 2006-08-31 2007-08-31 "PROCESSES FOR PRODUCTION OF A TRANSGENIC CORN PLANT".
DE602007012269T DE602007012269D1 (en) 2006-08-31 2007-08-31 PROCESS FOR PRODUCING TRANSGENIC PLANTS
JP2009526926A JP5350246B2 (en) 2006-08-31 2007-08-31 Method for producing a transgenic plant
ZA2009/01140A ZA200901140B (en) 2006-08-31 2009-02-17 Methods for producing producing transgenic plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US84151906P 2006-08-31 2006-08-31
US60/841,519 2006-08-31

Publications (2)

Publication Number Publication Date
WO2008028115A2 WO2008028115A2 (en) 2008-03-06
WO2008028115A3 true WO2008028115A3 (en) 2008-06-19

Family

ID=38779916

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/US2007/077361 Ceased WO2008028115A2 (en) 2006-08-31 2007-08-31 Methods for producing transgenic plants
PCT/US2007/077366 Ceased WO2008028119A2 (en) 2006-08-31 2007-08-31 Methods for rapidly transforming monocots
PCT/US2007/077370 Ceased WO2008028121A1 (en) 2006-08-31 2007-08-31 Plant transformation without selection

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/US2007/077366 Ceased WO2008028119A2 (en) 2006-08-31 2007-08-31 Methods for rapidly transforming monocots
PCT/US2007/077370 Ceased WO2008028121A1 (en) 2006-08-31 2007-08-31 Plant transformation without selection

Country Status (12)

Country Link
US (16) US8581035B2 (en)
EP (5) EP2057272B1 (en)
JP (3) JP5350246B2 (en)
CN (5) CN104357535B (en)
AT (1) ATE497008T1 (en)
AU (3) AU2007289105B2 (en)
BR (4) BRPI0716225B1 (en)
CA (8) CA2884046C (en)
DE (1) DE602007012269D1 (en)
MX (3) MX2009002250A (en)
WO (3) WO2008028115A2 (en)
ZA (3) ZA200901140B (en)

Families Citing this family (33)

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US20040210958A1 (en) * 2003-03-19 2004-10-21 Monsanto Technology Llc A Novel Culture Method for Corn Transformation
US7682829B2 (en) * 2003-05-30 2010-03-23 Monsanto Technology Llc Methods for corn transformation
JP4525314B2 (en) * 2004-11-26 2010-08-18 沖電気工業株式会社 Optical code division multiplexing transmission / reception method and optical code division multiplexing transmission / reception apparatus
CN104357535B (en) 2006-08-31 2018-03-27 孟山都技术有限公司 plant transformation without selection
CN105830921A (en) * 2007-08-31 2016-08-10 孟山都技术公司 Method and apparatus for substantially isolating plant tissues
JP2011120478A (en) * 2008-03-31 2011-06-23 Japan Tobacco Inc Agrobacterium-mediated method for producing transformed plant
US8952219B2 (en) 2009-06-25 2015-02-10 Syngenta Participations Ag Methods for Agrobacterium-mediated transformation of sugar cane
EP2475242B1 (en) * 2009-09-11 2017-03-22 Imperial Innovations Limited Method for producing leafy biomass in culture
PH12012501967A1 (en) 2010-06-24 2013-01-07 Univ Queensland Technology Methods for agrobacterium-mediated transformation of sugar cane
EP4059342A1 (en) 2010-12-17 2022-09-21 Monsanto Technology LLC Methods for improving competency of plant cells
US20130091606A1 (en) * 2011-06-24 2013-04-11 Bayer Cropscience Lp Methods and compositions for enhanced Agrobacterium-mediated transformation Efficiency
US11807846B2 (en) * 2012-04-29 2023-11-07 Monsanto Technology Llc Methods for transforming corn explants
US11713465B2 (en) 2013-11-21 2023-08-01 Monsanto Technology, Llc Methods for transforming wheat explants and compositions therefor
DE102013020605A1 (en) 2013-12-15 2015-06-18 Kws Saat Ag SELECTION-MARKER-FREE RHIZOBIACEAE-PROCESSED METHOD FOR PRODUCING A TRANSGENIC PLANT OF THE GENUS TRITICUM
CN103766221B (en) * 2014-02-10 2016-08-17 中国农业大学 A kind of monocotyledonous method for tissue culture
US9307708B2 (en) 2014-03-07 2016-04-12 Pioneer Hi-Bred International Inc Systems for extracting monocot embryos
GB2533129A (en) * 2014-12-11 2016-06-15 Alkion Biopharma Sas Bioreactor
WO2016105185A1 (en) * 2014-12-22 2016-06-30 Keygene N.V. Plant callus populations
MX2017016557A (en) * 2015-06-29 2018-05-11 Dow Agrosciences Llc System for automated explant preparation and method of use.
JP6716876B2 (en) * 2015-09-14 2020-07-01 住友ゴム工業株式会社 Method for producing transformed plant for producing genetically modified plant, and genetically modified transformed plant produced by this production method
ES2881392T3 (en) * 2015-10-30 2021-11-29 Pioneer Hi Bred Int Methods and compositions for rapid plant transformation
CN105660422B (en) * 2016-04-20 2017-09-29 遵义医学院 A kind of method for tissue culture of bletilla seedling
EP3400962A1 (en) 2017-05-09 2018-11-14 Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. Inhibitors of il-38 for use in treating and/or preventing cancer in a subject
ES2962273T3 (en) * 2017-06-02 2024-03-18 Fraunhofer Ges Forschung Procedure for the automated transformation of a package of plant cells
CN110226517B (en) * 2019-06-26 2021-06-01 北京市农林科学院 In-vitro regeneration method of onion and culture medium used by same
CN110607323A (en) * 2019-09-24 2019-12-24 四川育良生物科技有限公司 Agrobacterium tumefaciens-mediated rice genetic transformation method
AR127146A1 (en) * 2021-09-27 2023-12-20 Monsanto Technology Llc COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS
AR127147A1 (en) * 2021-09-27 2023-12-20 Monsanto Technology Llc COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS
WO2023049901A1 (en) * 2021-09-27 2023-03-30 Monsanto Technology Llc Compositions and methods for transformation of monocot seed excised embryo explants
US20240301435A1 (en) * 2021-09-27 2024-09-12 Monsanto Technology Llc Compositions and methods for transformation of monocot seed excised embryo explants
US20250051793A1 (en) * 2021-12-22 2025-02-13 BASF Agricultural Solutions Seed US LLC Improved screening method for genome edited events
AR128429A1 (en) * 2022-02-03 2024-05-08 Corteva Agriscience Llc PREPARATION OF MONOCOTYLEDON LEAF EXPLANTS
CA3238057A1 (en) * 2023-05-11 2025-06-25 Wisconsin Alumni Research Foundation Meristem transformation method using a liquid selection medium

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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