WO2008034896A3 - Identification and quantification of a plurality of biological (micro)organisms or their components - Google Patents

Identification and quantification of a plurality of biological (micro)organisms or their components Download PDF

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Publication number
WO2008034896A3
WO2008034896A3 PCT/EP2007/060037 EP2007060037W WO2008034896A3 WO 2008034896 A3 WO2008034896 A3 WO 2008034896A3 EP 2007060037 W EP2007060037 W EP 2007060037W WO 2008034896 A3 WO2008034896 A3 WO 2008034896A3
Authority
WO
WIPO (PCT)
Prior art keywords
solid support
amplicons
pcr
quantification
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/060037
Other languages
French (fr)
Other versions
WO2008034896A2 (en
WO2008034896A8 (en
Inventor
Isabelle Alexandre
Dieter Husar
Nathalie Zammatteo
Heinz Koehn
Jose Remacle
Sylvain Margaine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf Array Technologies SA
Original Assignee
Eppendorf Array Technologies SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Array Technologies SA filed Critical Eppendorf Array Technologies SA
Priority to EP07803579A priority Critical patent/EP2069069A2/en
Publication of WO2008034896A2 publication Critical patent/WO2008034896A2/en
Publication of WO2008034896A3 publication Critical patent/WO2008034896A3/en
Anticipated expiration legal-status Critical
Publication of WO2008034896A8 publication Critical patent/WO2008034896A8/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0803—Disc shape
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed is a system and method conducting real-time PCR. Capture molecules of a specific design are immobilized on a solid support, and contacted with amplicons produced in one or more PCR cycles. Detection of amplicons may take place during or between the PCR cycles while the solid support is in fluidic contact with the PCR solution. In one embodiment detection of the amplicons takes place when the solid support is not in fluidic contact with the PCR solution. In another embodiment, labeled amplicons are detected using an evanescent field while hybridized to capture probes immobilized on a solid support in the Real-time PCR reaction chamber. The method is suitable for the simultaneous detection and quantification of closely homologous target molecules.
PCT/EP2007/060037 2006-09-22 2007-09-21 Identification and quantification of a plurality of biological (micro)organisms or their components Ceased WO2008034896A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP07803579A EP2069069A2 (en) 2006-09-22 2007-09-21 Identification and quantification of a plurality of biological (micro)organisms or their components

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52615906A 2006-09-22 2006-09-22
US11/526,159 2006-09-22

Publications (3)

Publication Number Publication Date
WO2008034896A2 WO2008034896A2 (en) 2008-03-27
WO2008034896A3 true WO2008034896A3 (en) 2008-05-08
WO2008034896A8 WO2008034896A8 (en) 2009-11-05

Family

ID=38828646

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/060037 Ceased WO2008034896A2 (en) 2006-09-22 2007-09-21 Identification and quantification of a plurality of biological (micro)organisms or their components

Country Status (2)

Country Link
EP (1) EP2069069A2 (en)
WO (1) WO2008034896A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2107125A1 (en) * 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) Real-time PCR of targets on a micro-array
DE102009044431A1 (en) * 2009-11-05 2011-06-22 FRIZ Biochem Gesellschaft für Bioanalytik mbH, 82061 Device for carrying out a PCR
EP2441520A1 (en) 2010-10-12 2012-04-18 Eppendorf AG Real-time amplification and micro-array based detection of nucleic acid targets in a flow chip assay
US9347096B2 (en) * 2011-01-31 2016-05-24 Hitachi High-Technologies Corporation Genetic analyzer
KR101168165B1 (en) * 2012-02-29 2012-07-24 케이맥(주) Device chip for biological reaction
KR101184566B1 (en) * 2012-05-11 2012-09-20 케이맥(주) Method for integrated analysis of real-time pcr and dna chip
EP3064928B1 (en) * 2013-10-31 2019-04-17 Konica Minolta, Inc. Detection device, detection method using said detection device
KR101707456B1 (en) * 2015-10-01 2017-02-17 한국과학기술원 Appratus for film based pcr
CN108884430B (en) 2016-04-20 2019-11-19 希森美康株式会社 Nucleic acid analysis device and nucleic acid analysis method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6620623B1 (en) * 2002-05-06 2003-09-16 The University Of Chicago Biochip reader with enhanced illumination and bioarray positioning apparatus
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6620623B1 (en) * 2002-05-06 2003-09-16 The University Of Chicago Biochip reader with enhanced illumination and bioarray positioning apparatus
US20060088844A1 (en) * 2004-10-22 2006-04-27 Honeywell International Inc. Real-time PCR microarray based on evanescent wave biosensor

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HEALEY B G ET AL: "FIBEROPTIC DNA SENSOR ARRAY CAPABLE OF DETECTING POINT MUTATIONS", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 251, no. 2, 5 September 1997 (1997-09-05), pages 270 - 279, XP000703841, ISSN: 0003-2697 *
LEHR H-P ET AL: "REAL-TIME DETECTION OF NUCLEIC ACID INTERACTIONS BY TOTAL INTERNAL REFLECTION FLUORESCENCE", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 75, no. 10, 15 May 2003 (2003-05-15), pages 2414 - 2420, XP001170913, ISSN: 0003-2700 *
ROBIN HUI LIU ET AL: "SELF-CONTAINED, FULLY INTEGRATED BIOCHIP FOR SAMPLE PREPARATION, POLYMERASE CHAIN REACTION AMPLIFICATION, AND DNA MICROARRAY DETECTION", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 76, no. 7, 1 April 2004 (2004-04-01), pages 1824 - 1831, XP001196720, ISSN: 0003-2700 *
SCHENA MARK: "Microarray Analysis", 2003, JOHN WILEY & SONS, NEW JERSEY, XP002463284 *
STIMPSON D I ET AL: "REAL-TIME DETECTION OF DNA HYBRIDIZATION AND MELTING ON OLIGONUCLEOTIDE ARRAYS BY USING OPTICAL WAVE GUIDES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 92, July 1995 (1995-07-01), pages 6379 - 6383, XP002912130, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2008034896A2 (en) 2008-03-27
WO2008034896A8 (en) 2009-11-05
EP2069069A2 (en) 2009-06-17

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