WO2008052991A3 - Butanol production in a eukaryotic cell - Google Patents

Butanol production in a eukaryotic cell Download PDF

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Publication number
WO2008052991A3
WO2008052991A3 PCT/EP2007/061685 EP2007061685W WO2008052991A3 WO 2008052991 A3 WO2008052991 A3 WO 2008052991A3 EP 2007061685 W EP2007061685 W EP 2007061685W WO 2008052991 A3 WO2008052991 A3 WO 2008052991A3
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WO
WIPO (PCT)
Prior art keywords
dehydrogenase
coa
butanol
eukaryotic cell
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/061685
Other languages
French (fr)
Other versions
WO2008052991A2 (en
Inventor
Lourina Madeleine Raamsdonk
De Wilhelmus Theodorus An Laat
Van Den Marco Alexander Berg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
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DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Priority to BRPI0718101-9A priority Critical patent/BRPI0718101A2/en
Priority to US12/447,740 priority patent/US20100036174A1/en
Priority to EP07822039A priority patent/EP2097528A2/en
Publication of WO2008052991A2 publication Critical patent/WO2008052991A2/en
Publication of WO2008052991A3 publication Critical patent/WO2008052991A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
    • C12Y103/99002—Butyryl-CoA dehydrogenase (1.3.99.2), i.e. short chain acyl-CoA dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a transformed eukaryotic cell comprising one or more nucleotide sequence(s) encoding acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxybutyryl-CoA dehydratase, butyryl-CoA dehydrogenase, alcohol dehydrogenase or acetaldehyde dehydrogenase and/or NAD(P)H-dependent butanol dehydrogenase, whereby the nucleotide sequence(s) upon transformation of the cell confer(s) the cell the ability to produce butanol. The invention also relates to a process for the production of butanol.
PCT/EP2007/061685 2006-10-31 2007-10-30 Butanol production in a eukaryotic cell Ceased WO2008052991A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BRPI0718101-9A BRPI0718101A2 (en) 2006-10-31 2007-10-30 BUTHANOL PRODUCTION IN A EUCHARIOTIC CELL
US12/447,740 US20100036174A1 (en) 2006-10-31 2007-10-30 Butanol production in a eukaryotic cell
EP07822039A EP2097528A2 (en) 2006-10-31 2007-10-30 Butanol production in a eukaryotic cell

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US85537006P 2006-10-31 2006-10-31
US60/855,370 2006-10-31
EP06123259.1 2006-10-31
EP06123259 2006-10-31
US93502907P 2007-07-23 2007-07-23
EP07112954.8 2007-07-23
EP07112954 2007-07-23
US60/935,029 2007-07-23

Publications (2)

Publication Number Publication Date
WO2008052991A2 WO2008052991A2 (en) 2008-05-08
WO2008052991A3 true WO2008052991A3 (en) 2008-06-19

Family

ID=39271230

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/061685 Ceased WO2008052991A2 (en) 2006-10-31 2007-10-30 Butanol production in a eukaryotic cell

Country Status (3)

Country Link
US (1) US20100036174A1 (en)
EP (1) EP2097528A2 (en)
WO (1) WO2008052991A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9297028B2 (en) 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols
US8273558B2 (en) 2005-10-26 2012-09-25 Butamax(Tm) Advanced Biofuels Llc Fermentive production of four carbon alcohols
US20090155869A1 (en) * 2006-12-01 2009-06-18 Gevo, Inc. Engineered microorganisms for producing n-butanol and related methods
US8426173B2 (en) * 2007-05-02 2013-04-23 Butamax (Tm) Advanced Biofuels Llc Method for the production of 1-butanol
WO2009086423A2 (en) 2007-12-23 2009-07-09 Gevo, Inc. Yeast organism producing isobutanol at a high yield
US8455239B2 (en) 2007-12-23 2013-06-04 Gevo, Inc. Yeast organism producing isobutanol at a high yield
MY153731A (en) 2007-12-27 2015-03-13 Gevo Inc Recovery of higher alcohols from dilute aqueous solutions
DE102008004253B4 (en) * 2008-01-14 2011-07-28 Butalco Gmbh Increased production of acetyl coenzyme A
DK2262901T3 (en) 2008-03-05 2019-01-21 Genomatica Inc ORGANISMS PRODUCING PRIMARY ALCOHOL
BRPI0909989A2 (en) 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc recombinant yeast cell and method for producing a product
WO2010031772A2 (en) * 2008-09-16 2010-03-25 Dsm Ip Assets B.V. Alternative butanol production process in a microbial cell
MY176050A (en) 2009-04-30 2020-07-22 Genomatica Inc Organisms for the production of 1,3-butanediol
JP4821886B2 (en) 2009-06-04 2011-11-24 トヨタ自動車株式会社 Recombinant yeast and method for producing branched alcohol using the recombinant yeast
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
JP5787360B2 (en) * 2009-10-30 2015-09-30 株式会社ダイセル Genetically modified microorganisms provided with 1,3-butanediol production function and use thereof
KR20120120493A (en) * 2009-12-10 2012-11-01 게노마티카 인코포레이티드 Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol
NZ600509A (en) 2009-12-29 2014-08-29 Butamax Tm Advanced Biofuels Alcohol dehydrogenases (adh) useful for fermentive production of lower alkyl alcohols
CN102947457B (en) * 2010-06-18 2016-12-21 布特马斯先进生物燃料有限责任公司 Method and the system of undissolved solid was removed before extracting in production of butanol is fermented
US9689004B2 (en) 2012-03-23 2017-06-27 Butamax Advanced Biofuels Llc Acetate supplemention of medium for butanologens
US20160185633A1 (en) * 2014-12-30 2016-06-30 University Of Florida Research Foundation, Inc. Recovery of nutrients from water and wastewater by precipitation as struvite
CN114507608B (en) * 2020-11-17 2023-08-22 中国科学院天津工业生物技术研究所 Recombinant filamentous fungus for producing ethanol in online granule body and construction and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0088656A1 (en) * 1982-03-08 1983-09-14 Institut Français du Pétrole Production of mutants of clostridium acetobutylicum with high productivity of butanol and acetone, mutants obtained and use of these mutants in the joint production of butanol and acetone
WO1998051813A1 (en) * 1997-05-14 1998-11-19 The Board Of Trustees Of The University Of Illinois A METHOD OF PRODUCING BUTANOL USING A MUTANT STRAIN OF $i(CLOSTRIDIUM BEIJERINCKII)
US6960465B1 (en) * 2001-06-27 2005-11-01 Northwestern University Increased cell resistance to toxic organic substances
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0088656A1 (en) * 1982-03-08 1983-09-14 Institut Français du Pétrole Production of mutants of clostridium acetobutylicum with high productivity of butanol and acetone, mutants obtained and use of these mutants in the joint production of butanol and acetone
WO1998051813A1 (en) * 1997-05-14 1998-11-19 The Board Of Trustees Of The University Of Illinois A METHOD OF PRODUCING BUTANOL USING A MUTANT STRAIN OF $i(CLOSTRIDIUM BEIJERINCKII)
US6960465B1 (en) * 2001-06-27 2005-11-01 Northwestern University Increased cell resistance to toxic organic substances
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BERMEJO L L ET AL: "Expression of Clostridium acetobutylicum ATCC 824 genes in Escherichia coli for acetone production and acetate detoxification", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, no. 3, March 1998 (1998-03-01), pages 1079 - 1085, XP002334443, ISSN: 0099-2240 *
CORNILLOT E ET AL: "The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain", JOURNAL OF BACTERIOLOGY, vol. 179, no. 17, 1997, pages 5442 - 5447, XP002412768, ISSN: 0021-9193 *
FONTAINE L ET AL: "Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824", JOURNAL OF BACTERIOLOGY, vol. 184, no. 3, February 2002 (2002-02-01), pages 821 - 830, XP002412767, ISSN: 0021-9193 *
JONES D T ET AL: "Acetone-butanol fermentation revisited.", MICROBIOLOGICAL REVIEWS, vol. 50, no. 4, December 1986 (1986-12-01), pages 484 - 524, XP002425366, ISSN: 0146-0749 *
WOODS D R: "The genetic engineering of microbial solvent production", TRENDS IN BIOTECHNOLOGY, vol. 13, no. 7, July 1995 (1995-07-01), pages 259 - 264, XP004207180, ISSN: 0167-7799 *

Also Published As

Publication number Publication date
US20100036174A1 (en) 2010-02-11
WO2008052991A2 (en) 2008-05-08
EP2097528A2 (en) 2009-09-09

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