WO2008063892A3 - Rationally designed media for cell culture - Google Patents

Rationally designed media for cell culture Download PDF

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Publication number
WO2008063892A3
WO2008063892A3 PCT/US2007/083947 US2007083947W WO2008063892A3 WO 2008063892 A3 WO2008063892 A3 WO 2008063892A3 US 2007083947 W US2007083947 W US 2007083947W WO 2008063892 A3 WO2008063892 A3 WO 2008063892A3
Authority
WO
WIPO (PCT)
Prior art keywords
concentration
amino acid
cell
media
cell culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/083947
Other languages
French (fr)
Other versions
WO2008063892A2 (en
Inventor
Yen-Tung Luan
Wenge Wang
Ryan Nolan
Denis Drapeau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth LLC
Original Assignee
Wyeth LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=39430451&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2008063892(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to MX2009004974A priority Critical patent/MX2009004974A/en
Priority to CA2668771A priority patent/CA2668771C/en
Priority to ES07868688.8T priority patent/ES2538986T3/en
Priority to EP07868688.8A priority patent/EP2078071B1/en
Priority to RU2009114855/10A priority patent/RU2520810C2/en
Priority to AU2007323978A priority patent/AU2007323978B2/en
Priority to SI200731638T priority patent/SI2078071T1/en
Priority to BRPI0718713A priority patent/BRPI0718713B1/en
Application filed by Wyeth LLC filed Critical Wyeth LLC
Priority to JP2009536462A priority patent/JP5564259B2/en
Priority to HK09112222.6A priority patent/HK1132300B/en
Priority to KR1020097011505A priority patent/KR101523782B1/en
Priority to PL07868688T priority patent/PL2078071T3/en
Priority to DK07868688.8T priority patent/DK2078071T3/en
Publication of WO2008063892A2 publication Critical patent/WO2008063892A2/en
Publication of WO2008063892A3 publication Critical patent/WO2008063892A3/en
Priority to NO20091607A priority patent/NO20091607L/en
Priority to IL198486A priority patent/IL198486A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest. The rationally designed media may contain a baseline- adjusted concentration, A, of at least one amino acid that is calculated according to the formula A=[(M*X)+(N*P)+(Y*M*X)]*F, wherein X is the concentration of the amino acid that is used per unit of cell mass, P is the concentration of the amino acid that is used for incorporation into the polypeptide of interest per unit of polypeptide titer, M is the multiplier for the desired peak cell density of the cell culture, N is the multiplier for the desired concentration of the polypeptide of interest, Y is the cell maintenance factor; and F is the baseline factor. This rationally designed media may also be used to produce a starting cell culture medium comprising a concentration, B, of at least one amino acid according to the formula B=[A- (Z*V)]/(1-V), wherein Z is a concentration of the amino acid in the feeding cell culture medium, and V is a volume of the feeding culture medium as a proportion of the desired cell culture medium volume.
PCT/US2007/083947 2006-11-08 2007-11-07 Rationally designed media for cell culture Ceased WO2008063892A2 (en)

Priority Applications (15)

Application Number Priority Date Filing Date Title
DK07868688.8T DK2078071T3 (en) 2006-11-08 2007-11-07 Rationally crafted cell culture media
CA2668771A CA2668771C (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
ES07868688.8T ES2538986T3 (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
EP07868688.8A EP2078071B1 (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
RU2009114855/10A RU2520810C2 (en) 2006-11-08 2007-11-07 Rationally developed media for cell cultivation
AU2007323978A AU2007323978B2 (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
SI200731638T SI2078071T1 (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
BRPI0718713A BRPI0718713B1 (en) 2006-11-08 2007-11-07 method of producing a polypeptide in a cell culture; polypeptide produced according to the method; pharmaceutical composition comprising the polypeptide; cell culture medium; cell culture medium for use in the production of a polypeptide of interest; method for determining an optimized concentration of an amino acid used in a cell culture medium for the production of a polypeptide of interest in a cell culture
JP2009536462A JP5564259B2 (en) 2006-11-08 2007-11-07 Reasonably designed medium for cell culture
MX2009004974A MX2009004974A (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture.
PL07868688T PL2078071T3 (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
KR1020097011505A KR101523782B1 (en) 2006-11-08 2007-11-07 Reasonably designed media for cell culture
HK09112222.6A HK1132300B (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture
NO20091607A NO20091607L (en) 2006-11-08 2009-04-22 Rationally designed media for cell culture
IL198486A IL198486A (en) 2006-11-08 2009-04-30 Rationally designed media for cell culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US85828906P 2006-11-08 2006-11-08
US60/858,289 2006-11-08

Publications (2)

Publication Number Publication Date
WO2008063892A2 WO2008063892A2 (en) 2008-05-29
WO2008063892A3 true WO2008063892A3 (en) 2008-08-28

Family

ID=39430451

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/083947 Ceased WO2008063892A2 (en) 2006-11-08 2007-11-07 Rationally designed media for cell culture

Country Status (25)

Country Link
US (2) US8232075B2 (en)
EP (2) EP2921552A1 (en)
JP (2) JP5564259B2 (en)
KR (1) KR101523782B1 (en)
CN (2) CN101535469A (en)
AR (1) AR063623A1 (en)
AU (1) AU2007323978B2 (en)
BR (1) BRPI0718713B1 (en)
CA (1) CA2668771C (en)
CL (1) CL2007003221A1 (en)
CR (1) CR10781A (en)
DK (1) DK2078071T3 (en)
EC (1) ECSP099313A (en)
ES (1) ES2538986T3 (en)
IL (1) IL198486A (en)
MX (1) MX2009004974A (en)
NO (1) NO20091607L (en)
PE (1) PE20081655A1 (en)
PL (1) PL2078071T3 (en)
PT (1) PT2078071E (en)
RU (1) RU2520810C2 (en)
SI (1) SI2078071T1 (en)
TW (1) TW200827445A (en)
WO (1) WO2008063892A2 (en)
ZA (1) ZA200903170B (en)

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US11225513B2 (en) 2009-08-06 2022-01-18 Genentech, Inc. Method to improve virus filtration capacity

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CA2668771C (en) * 2006-11-08 2016-03-15 Wyeth Rationally designed media for cell culture
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EP2188371B1 (en) 2007-08-09 2017-12-20 Wyeth LLC Use of perfusion to enhance production of fed-batch cell culture in bioreactors
US8178318B2 (en) * 2008-08-06 2012-05-15 Praxair Technology, Inc. Method for controlling pH, osmolality and dissolved carbon dioxide levels in a mammalian cell culture process to enhance cell viability and biologic product yield
JP2011530290A (en) * 2008-08-06 2011-12-22 プラクスエア・テクノロジー・インコーポレイテッド Systems and methods for controlling mammalian cell culture processes
US20110262965A1 (en) * 2010-04-23 2011-10-27 Life Technologies Corporation Cell culture medium comprising small peptides
CA2795461C (en) 2010-04-26 2018-04-24 Novartis Ag Improved cell cultivation process
PT2563904E (en) * 2010-04-26 2015-05-12 Novartis Ag Improved cell culture medium
ES2434737T3 (en) * 2010-10-27 2013-12-17 Lonza Biologics Plc. Rapid targeted cell selection (cell lines) procedure
PT105484A (en) 2011-01-14 2012-07-16 Univ Nova De Lisboa A FUNCTIONAL ENVIRONMENTAL METHOD FOR CELLULAR CULTURAL MEDIA ENGINEERING
KR101662412B1 (en) 2011-07-01 2016-10-04 암젠 인크 Mammalian cell culture
WO2013009648A2 (en) 2011-07-08 2013-01-17 Momenta Pharmaceuticals, Inc. Cell culture process
AU2014240596B2 (en) * 2013-03-14 2018-09-27 Amgen Inc. Removal of leaked affinity purification ligand
AR095196A1 (en) 2013-03-15 2015-09-30 Regeneron Pharma SERUM FREE CELL CULTIVATION MEDIA
TWI870789B (en) 2015-08-04 2025-01-21 美商再生元醫藥公司 Taurine supplemented cell culture medium and methods of use
EP3377613A1 (en) 2015-11-17 2018-09-26 Pfizer Inc Media and fermentation methods for producing polysaccharides in bacterial cell culture
CA3019753C (en) * 2016-04-05 2021-10-26 Pfizer Inc. Cell culture process
CN106096077B (en) * 2016-05-26 2019-05-21 新乡医学院 Media Optimization Methods
JP6314201B1 (en) * 2016-11-21 2018-04-18 テラファーマ株式会社 Dendritic cell washing solution, dendritic cell washing method using the same, and method for preparing dendritic cell-containing composition
RU2759602C2 (en) * 2017-02-08 2021-11-16 Пфайзер Инк. Process of large-scale production of capped and uncapped antibody cysteines and their application for therapeutic protein conjugation
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
SG11202001127TA (en) * 2017-10-16 2020-03-30 Regeneron Pharma In situ raman spectroscopy systems and methods for controlling process variables in cell cultures
US12480091B2 (en) * 2017-11-16 2025-11-25 Life Technologies Corporation Streamlined methods for making liquid media
JP6385607B2 (en) * 2018-02-13 2018-09-05 テラファーマ株式会社 Dendritic cell vaccine
RS65472B1 (en) 2018-07-03 2024-05-31 Bristol Myers Squibb Co Methods of producing recombinant proteins
CN108823173A (en) * 2018-07-24 2018-11-16 郑州伊美诺生物技术有限公司 Supplemented medium and preparation method thereof for Hybridoma Cell Culture
CN109576203B (en) * 2018-12-20 2021-03-23 吉林省浦生泰生物技术有限责任公司 Composite probiotic culture medium based on traditional Chinese medicine additive and preparation method thereof
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
CN111748527B (en) * 2020-05-15 2021-01-29 上海多宁生物科技有限公司 Chemical component limited efficient feeding culture medium and preparation method and application thereof
CN113450882B (en) * 2020-09-27 2022-03-01 深圳太力生物技术有限责任公司 Artificial intelligence-based basic culture medium formula development method and system
JP7822149B2 (en) 2020-10-02 2026-03-02 ファイザー・インク Cell culture process for RSV F protein production
GB202105424D0 (en) * 2021-04-16 2021-06-02 UCB Biopharma SRL Cell culture processes
WO2022254319A1 (en) 2021-06-01 2022-12-08 Pfizer Inc. Cell culture method for producing sfgfr3 polypeptide
JP7190534B1 (en) * 2021-06-14 2022-12-15 アサヒ飲料株式会社 Microorganism detection method
JP7820943B2 (en) * 2021-10-28 2026-02-26 花王株式会社 Method for producing VHH antibody
EP4582528A4 (en) * 2022-10-17 2025-12-17 Phc Holdings Corp CULTURE CONTROL DEVICE AND CULTURE CONTROL METHOD
CN116334007A (en) * 2023-04-10 2023-06-27 珠海市丽珠单抗生物技术有限公司 A kind of packaging method of lentivirus
KR20240177497A (en) * 2023-06-20 2024-12-27 (주)엑셀세라퓨틱스 A platform system for designing medium formulations tailored to cell-specific characteristics

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Publication number Priority date Publication date Assignee Title
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Also Published As

Publication number Publication date
SI2078071T1 (en) 2015-05-29
NO20091607L (en) 2009-05-28
CN104152395A (en) 2014-11-19
KR101523782B1 (en) 2015-05-28
ZA200903170B (en) 2010-03-31
IL198486A0 (en) 2011-08-01
BRPI0718713A2 (en) 2013-11-26
DK2078071T3 (en) 2015-04-20
EP2078071B1 (en) 2015-03-18
PE20081655A1 (en) 2008-12-03
RU2009114855A (en) 2010-12-20
HK1132300A1 (en) 2010-02-19
JP2014128276A (en) 2014-07-10
BRPI0718713B1 (en) 2019-12-24
CA2668771A1 (en) 2008-05-29
CA2668771C (en) 2016-03-15
AU2007323978B2 (en) 2012-08-16
CR10781A (en) 2009-06-09
RU2520810C2 (en) 2014-06-27
KR20090079254A (en) 2009-07-21
PL2078071T3 (en) 2015-07-31
AU2007323978A1 (en) 2008-05-29
US20130084593A1 (en) 2013-04-04
PT2078071E (en) 2015-07-16
CL2007003221A1 (en) 2008-05-16
IL198486A (en) 2015-06-30
CN104152395B (en) 2020-03-10
US8232075B2 (en) 2012-07-31
WO2008063892A2 (en) 2008-05-29
ECSP099313A (en) 2009-06-30
JP2010508853A (en) 2010-03-25
US20080108553A1 (en) 2008-05-08
MX2009004974A (en) 2009-05-20
EP2921552A1 (en) 2015-09-23
EP2078071A2 (en) 2009-07-15
AR063623A1 (en) 2009-02-04
JP5564259B2 (en) 2014-07-30
CN101535469A (en) 2009-09-16
ES2538986T3 (en) 2015-06-25
TW200827445A (en) 2008-07-01

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