WO2008084087A3 - Dbait and its standalone uses thereof - Google Patents

Dbait and its standalone uses thereof Download PDF

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Publication number
WO2008084087A3
WO2008084087A3 PCT/EP2008/050265 EP2008050265W WO2008084087A3 WO 2008084087 A3 WO2008084087 A3 WO 2008084087A3 EP 2008050265 W EP2008050265 W EP 2008050265W WO 2008084087 A3 WO2008084087 A3 WO 2008084087A3
Authority
WO
WIPO (PCT)
Prior art keywords
repair
standalone
discloses
dna
dsb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/050265
Other languages
French (fr)
Other versions
WO2008084087A2 (en
Inventor
Marie Dutreix
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut Curie
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut Curie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Institut Curie filed Critical Centre National de la Recherche Scientifique CNRS
Priority to EA200900989A priority Critical patent/EA016053B1/en
Priority to US12/520,961 priority patent/US8093366B2/en
Priority to CA2673972A priority patent/CA2673972C/en
Priority to AU2008204486A priority patent/AU2008204486C1/en
Priority to JP2009545182A priority patent/JP5462632B2/en
Priority to EP08701411.4A priority patent/EP2102343B1/en
Priority to CN200880002041.7A priority patent/CN101622351B/en
Publication of WO2008084087A2 publication Critical patent/WO2008084087A2/en
Publication of WO2008084087A3 publication Critical patent/WO2008084087A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/13—Decoys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/312—Phosphonates
    • C12N2310/3125—Methylphosphonates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to compositions and methods for interfering with the DNA repair of double strand breaks (DSBs). The invention discloses double-stranded nucleic acid molecules that act as baits and hijack the holocomplex of enzymes responsible of DNA DSB sensing, signaling and/or repair pathways, in particular the non homologous end joining (NHEJ) pathway of DSB repair. The invention discloses the use of these molecules as a standalone anticancer drug in an efficient amount to be introduced in the tumor cell nuclei in order to trigger their death.
PCT/EP2008/050265 2007-01-12 2008-01-11 Dbait and its standalone uses thereof Ceased WO2008084087A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EA200900989A EA016053B1 (en) 2007-01-12 2008-01-11 Use of a nucleic acid molecule comprising a double stranded portion for treating proliferative disorders
US12/520,961 US8093366B2 (en) 2007-01-12 2008-01-11 Dbait and standalone uses thereof
CA2673972A CA2673972C (en) 2007-01-12 2008-01-11 Dbait and its standalone uses thereof
AU2008204486A AU2008204486C1 (en) 2007-01-12 2008-01-11 Dbait and its standalone uses thereof
JP2009545182A JP5462632B2 (en) 2007-01-12 2008-01-11 Dbait and its single use
EP08701411.4A EP2102343B1 (en) 2007-01-12 2008-01-11 Dbait and its standalone uses thereof
CN200880002041.7A CN101622351B (en) 2007-01-12 2008-01-11 Dbait and its standalone uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07300728.8 2007-01-12
EP07300728A EP1944369A1 (en) 2007-01-12 2007-01-12 Dbait and its standalone uses thereof

Publications (2)

Publication Number Publication Date
WO2008084087A2 WO2008084087A2 (en) 2008-07-17
WO2008084087A3 true WO2008084087A3 (en) 2009-01-08

Family

ID=38515555

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/050265 Ceased WO2008084087A2 (en) 2007-01-12 2008-01-11 Dbait and its standalone uses thereof

Country Status (8)

Country Link
US (1) US8093366B2 (en)
EP (2) EP1944369A1 (en)
JP (1) JP5462632B2 (en)
CN (1) CN101622351B (en)
AU (1) AU2008204486C1 (en)
CA (1) CA2673972C (en)
EA (1) EA016053B1 (en)
WO (1) WO2008084087A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109321585B (en) * 2018-09-30 2021-08-06 山东大学 A method for improving the mutagenesis breeding efficiency of prokaryotic microorganisms by using T4 deoxyribonucleic acid ligase

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5860874B2 (en) 2010-06-22 2016-02-16 デエヌア・テラプーティックＤｎａｔｈｅｒａｐｅｕｔｉｃｓ Optimized in vivo delivery system using endosomal lytic agents for nucleic acid conjugates
AU2015202211B2 (en) * 2010-06-22 2016-09-01 Centre National De La Recherche Scientifique Optimized in vivo delivery system with endosomolytic agents for nucleic acid conjugates
EP2527440A1 (en) * 2011-05-27 2012-11-28 Institut Curie Cancer treatment by combining DNA molecules mimicking double strand breaks with hyperthermia
AU2016296905B2 (en) 2015-07-23 2018-07-05 Centre National De La Recherche Scientifique Use of a combination of Dbait molecule and parp inhibitors to treat cancer
KR102441432B1 (en) * 2016-03-01 2022-09-07 옹쎄오 Cancer treatment by systemic administration of DBD molecules
WO2017186882A1 (en) * 2016-04-29 2017-11-02 Onxeo A method of predicting a response to an anti-tumor treatment by means of signal interfering dna molecules
WO2018162439A1 (en) 2017-03-08 2018-09-13 Onxeo New predictive biomarker for the sensitivity to a treatment of cancer with a dbait molecule
WO2018231752A1 (en) * 2017-06-12 2018-12-20 University Of Miami Sting-dependent activators for treatment of disease
CN107793468A (en) * 2017-06-26 2018-03-13 上海悦良生物科技有限公司 A kind of method for detecting DNA damage
EP3461488A1 (en) 2017-09-27 2019-04-03 Onxeo Combination of a dbait molecule and a hdac inhibitor for treating cancer
CA3092779A1 (en) * 2018-03-13 2019-09-19 Onxeo A dbait molecule against acquired resistance in the treatment of cancer
BR112021012066A2 (en) 2018-12-21 2021-11-03 Onxeo New conjugated nucleic acid molecules and their uses
EA202192575A1 (en) 2019-03-21 2022-01-14 Онксео DBAIT COMPOUNDS IN COMBINATION WITH KINASE INHIBITORS FOR CANCER TREATMENT
EP4075980B1 (en) 2019-12-18 2025-07-23 Stinginn LLC Substituted 1,2, 4-triazoles and methods of use
WO2021148581A1 (en) 2020-01-22 2021-07-29 Onxeo Novel dbait molecule and its use
US20230095192A1 (en) * 2020-03-03 2023-03-30 University Of Washington Synthetic agonists of dna-pk and their use
US11897888B1 (en) 2020-04-30 2024-02-13 Stinginn Llc Small molecular inhibitors of sting signaling compositions and methods of use
TW202210633A (en) 2020-06-05 2022-03-16 法商昂席歐公司 A dbait molecule in combination with kras inhibitor for the treatment of cancer
TW202214857A (en) 2020-06-19 2022-04-16 法商昂席歐公司 New conjugated nucleic acid molecules and their uses
KR20240133795A (en) 2021-12-16 2024-09-04 발레리오 테라퓨틱스 Novel conjugated nucleic acid molecules and uses thereof
WO2024240858A1 (en) 2023-05-23 2024-11-28 Valerio Therapeutics Protac molecules directed against dna damage repair system and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005040378A1 (en) * 2003-10-24 2005-05-06 Institut Curie Nucleic acids useful for triggering tumor cell lethality

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005247807A (en) * 2004-03-08 2005-09-15 Japan Science & Technology Agency Cancer treatment composition using NSAID
US20060276423A1 (en) * 2005-04-18 2006-12-07 Rachel Altura Survivin-directed RNA interference-compositions and methods

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005040378A1 (en) * 2003-10-24 2005-05-06 Institut Curie Nucleic acids useful for triggering tumor cell lethality

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LI Z. ET AL.: "Targeting Ku protein for sensitizing of breast cancer cells to DNA damage", INTERNATIONAL JOURNAL OF MEDICINE, vol. 14, no. 2, August 2004 (2004-08-01), pages 153 - 159, XP008084143 *
YOO S. AND DYNAN W.S.: "Characterization of the RNA binding properties of Ku protein", BIOCHEMISTRY, vol. 37, 1998, pages 1336 - 1343, XP002452541 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109321585B (en) * 2018-09-30 2021-08-06 山东大学 A method for improving the mutagenesis breeding efficiency of prokaryotic microorganisms by using T4 deoxyribonucleic acid ligase

Also Published As

Publication number Publication date
AU2008204486C1 (en) 2013-05-16
EA200900989A1 (en) 2009-12-30
EP2102343B1 (en) 2015-07-08
AU2008204486B2 (en) 2012-11-29
EP1944369A1 (en) 2008-07-16
CA2673972A1 (en) 2008-07-17
WO2008084087A2 (en) 2008-07-17
EP2102343A2 (en) 2009-09-23
CA2673972C (en) 2016-03-22
CN101622351B (en) 2014-05-14
US8093366B2 (en) 2012-01-10
JP5462632B2 (en) 2014-04-02
EP1944369A9 (en) 2008-10-15
AU2008204486A1 (en) 2008-07-17
EA016053B1 (en) 2012-01-30
JP2010515707A (en) 2010-05-13
CN101622351A (en) 2010-01-06
US20100022622A1 (en) 2010-01-28

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