WO2008086381A3 - Isothermal dna amplification - Google Patents

Isothermal dna amplification Download PDF

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Publication number
WO2008086381A3
WO2008086381A3 PCT/US2008/050555 US2008050555W WO2008086381A3 WO 2008086381 A3 WO2008086381 A3 WO 2008086381A3 US 2008050555 W US2008050555 W US 2008050555W WO 2008086381 A3 WO2008086381 A3 WO 2008086381A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna amplification
isothermal dna
endonuclease
target dna
isothermal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/050555
Other languages
French (fr)
Other versions
WO2008086381A2 (en
Inventor
John R Nelson
Robert Scott Duthie
Carl Williams Fuller
Gregory A Grossmann
Anuradha Sekher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Priority to EP08705788.1A priority Critical patent/EP2106452B1/en
Publication of WO2008086381A2 publication Critical patent/WO2008086381A2/en
Publication of WO2008086381A3 publication Critical patent/WO2008086381A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA.
PCT/US2008/050555 2007-01-10 2008-01-09 Isothermal dna amplification Ceased WO2008086381A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08705788.1A EP2106452B1 (en) 2007-01-10 2008-01-09 Isothermal dna amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/621,703 2007-01-10
US11/621,703 US8202972B2 (en) 2007-01-10 2007-01-10 Isothermal DNA amplification

Publications (2)

Publication Number Publication Date
WO2008086381A2 WO2008086381A2 (en) 2008-07-17
WO2008086381A3 true WO2008086381A3 (en) 2008-09-18

Family

ID=39493389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/050555 Ceased WO2008086381A2 (en) 2007-01-10 2008-01-09 Isothermal dna amplification

Country Status (3)

Country Link
US (2) US8202972B2 (en)
EP (1) EP2106452B1 (en)
WO (1) WO2008086381A2 (en)

Families Citing this family (29)

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US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US9279150B2 (en) 2007-01-10 2016-03-08 General Electric Company Mutant endonuclease V enzymes and applications thereof
US20130323795A1 (en) 2007-01-10 2013-12-05 General Electric Company Endonuclase-assisted isothermal amplification using contamination-free reagents
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8143006B2 (en) 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
US20110003701A1 (en) * 2008-02-27 2011-01-06 454 Life Sciences Corporation System and method for improved processing of nucleic acids for production of sequencable libraries
US20110171649A1 (en) * 2008-09-10 2011-07-14 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
EP2483425B1 (en) 2009-09-28 2016-08-24 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
EP2369325A1 (en) 2010-03-12 2011-09-28 Eppendorf Ag Array analysis for online detection
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
DK2718260T3 (en) 2011-06-08 2018-11-19 Life Technologies Corp DESIGN AND DEVELOPMENT OF NEW DETERGENTS FOR USE IN PCR SYSTEMS
WO2012178187A1 (en) 2011-06-23 2012-12-27 Paul Yager Reagent patterning in capillarity-based analyzers and associated systems and methods
KR102362649B1 (en) 2012-04-09 2022-02-14 엔바이로로직스 인크. Compositions and methods for quantifying a nucleic acid sequence in a sample
US9777319B2 (en) * 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
WO2014004852A2 (en) * 2012-06-29 2014-01-03 General Electric Company Kit for isothermal dna amplification starting from an rna template
CN105050720A (en) 2013-01-22 2015-11-11 华盛顿大学商业化中心 Sequential delivery of fluid volumes and associated devices, systems and methods
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
EP3033431B1 (en) * 2013-08-13 2019-10-02 General Electric Company Endonuclease-assisted isothermal amplification using contamination-free reagents
EP3058100A4 (en) * 2013-10-18 2017-04-19 California Institute of Technology Enhanced nucleic acid identification and detection
CN106458885B (en) 2013-10-25 2019-12-10 生命技术公司 Novel compounds for use in polymerase chain reaction systems and uses thereof
US20150167065A1 (en) * 2013-12-13 2015-06-18 General Electric Company Isothermal amplification of nucleic acids within a porous matrix
US9850525B2 (en) 2014-01-29 2017-12-26 Agilent Technologies, Inc. CAS9-based isothermal method of detection of specific DNA sequence
EP3277837A1 (en) * 2015-03-31 2018-02-07 Qiagen GmbH Efficiency improving ligation methods
WO2018225772A1 (en) * 2017-06-07 2018-12-13 タカラバイオ株式会社 Oligonucleotide preservation method
WO2020092268A2 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Thermokinetically balanced isothermal amplification of nucleic acid sequences
KR102249111B1 (en) * 2019-04-22 2021-05-07 포항공과대학교 산학협력단 Novel Probe Set for Isothermal One-pot Reaction and Uses Thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1167524A1 (en) * 1999-03-19 2002-01-02 Takara Shuzo Co, Ltd. Method for amplifying nucleic acid sequence
WO2002036821A2 (en) * 2000-11-03 2002-05-10 University College Cork - National University Of Ireland, Cork; Method for the amplification and optional characterisation of nucleic acids
US20030104431A1 (en) * 2001-07-15 2003-06-05 Keck Graduate Institute Nucleic acid amplification using nicking agents
WO2004003232A1 (en) * 2002-06-28 2004-01-08 Hibergen Genomics Limited Method for the characterisation of nucleic acid molecules
US20070020639A1 (en) * 2005-07-20 2007-01-25 Affymetrix, Inc. Isothermal locus specific amplification
WO2008013010A1 (en) * 2006-07-26 2008-01-31 Nishikawa Rubber Co., Ltd. Method for amplification of nucleotide sequence

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US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
JP2000516468A (en) * 1996-08-14 2000-12-12 ライフ テクノロジーズ，インコーポレイテッド Stable compositions for nucleic acid amplification and sequencing
US6951722B2 (en) * 1999-03-19 2005-10-04 Takara Bio Inc. Method for amplifying nucleic acid sequence
US7198894B2 (en) * 2000-12-01 2007-04-03 Cornell Research Foundation, Inc. Detection of nucleic acid differences using combined endonuclease cleavage and ligation reactions
US7273730B2 (en) * 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
WO2005017173A1 (en) * 2003-07-29 2005-02-24 Sigma Aldrich Co. Methods and compositions for amplification of dna
EP1883707B1 (en) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US20070148636A1 (en) * 2005-12-23 2007-06-28 Song Min-Sun Method, compositions and kits for preparation of nucleic acids

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1167524A1 (en) * 1999-03-19 2002-01-02 Takara Shuzo Co, Ltd. Method for amplifying nucleic acid sequence
WO2002036821A2 (en) * 2000-11-03 2002-05-10 University College Cork - National University Of Ireland, Cork; Method for the amplification and optional characterisation of nucleic acids
US20030104431A1 (en) * 2001-07-15 2003-06-05 Keck Graduate Institute Nucleic acid amplification using nicking agents
WO2004003232A1 (en) * 2002-06-28 2004-01-08 Hibergen Genomics Limited Method for the characterisation of nucleic acid molecules
US20070020639A1 (en) * 2005-07-20 2007-01-25 Affymetrix, Inc. Isothermal locus specific amplification
WO2008013010A1 (en) * 2006-07-26 2008-01-31 Nishikawa Rubber Co., Ltd. Method for amplification of nucleotide sequence

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 200818, Derwent World Patents Index; AN 2008-C48048, XP002484971 *
TERRANCE WALKER G: "ISOTHERMAL IN VITRO AMPLIFICATION OF DNA BY A RESTRICTION ENZYME/ DNA POLYMERASE SYSTEM", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, vol. 89, no. 1, 1 January 1992 (1992-01-01), pages 392 - 396, XP000368694, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
US20090011472A1 (en) 2009-01-08
EP2106452A2 (en) 2009-10-07
WO2008086381A2 (en) 2008-07-17
US20120196330A1 (en) 2012-08-02
EP2106452B1 (en) 2017-06-21
US8202972B2 (en) 2012-06-19
US9951379B2 (en) 2018-04-24

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