WO2008114656A1 - リパーゼ粉末製剤、その製造方法及び使用 - Google Patents
リパーゼ粉末製剤、その製造方法及び使用 Download PDFInfo
- Publication number
- WO2008114656A1 WO2008114656A1 PCT/JP2008/054448 JP2008054448W WO2008114656A1 WO 2008114656 A1 WO2008114656 A1 WO 2008114656A1 JP 2008054448 W JP2008054448 W JP 2008054448W WO 2008114656 A1 WO2008114656 A1 WO 2008114656A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- production
- lipase
- lipase preparation
- powdery
- powdery lipase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6454—Glycerides by esterification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fats And Perfumes (AREA)
Abstract
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW097108453A TWI438279B (zh) | 2007-03-16 | 2008-03-11 | 脂肪酶粉末製劑,其製造方法及使用 |
| CA2680863A CA2680863C (en) | 2007-03-16 | 2008-03-12 | Powdery lipase preparation, method for producing the same and use thereof |
| EP08721864.0A EP2141229B1 (en) | 2007-03-16 | 2008-03-12 | Powdery lipase preparation, method for production thereof, and use thereof |
| CN200880014725A CN101675159A (zh) | 2007-03-16 | 2008-03-12 | 脂肪酶粉末制剂、其制备方法及应用 |
| DK08721864.0T DK2141229T3 (en) | 2007-03-16 | 2008-03-12 | Powdery lipase preparation, process for their preparation and use thereof |
| US12/531,327 US8921081B2 (en) | 2007-03-16 | 2008-03-12 | Powdery lipase preparation, method for producing the same and use thereof |
| AU2008227690A AU2008227690B2 (en) | 2007-03-16 | 2008-03-12 | Powdery lipase preparation, method for producing the same and use thereof |
| ES08721864.0T ES2537325T3 (es) | 2007-03-16 | 2008-03-12 | Preparación de lipasa en polvo, método para la producción de la misma y uso de la misma |
| JP2009505150A JP5145609B2 (ja) | 2007-03-16 | 2008-03-12 | リパーゼ粉末製剤、その製造方法及び使用 |
| US14/573,383 US20150104839A1 (en) | 2007-03-16 | 2014-12-17 | Powdery lipase preparation, method for producing the same and use thereof |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007-069189 | 2007-03-16 | ||
| JP2007069189 | 2007-03-16 |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US12/531,327 A-371-Of-International US8921081B2 (en) | 2007-03-16 | 2008-03-12 | Powdery lipase preparation, method for producing the same and use thereof |
| US14/573,383 Continuation US20150104839A1 (en) | 2007-03-16 | 2014-12-17 | Powdery lipase preparation, method for producing the same and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2008114656A1 true WO2008114656A1 (ja) | 2008-09-25 |
Family
ID=39765764
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/JP2008/054448 Ceased WO2008114656A1 (ja) | 2007-03-16 | 2008-03-12 | リパーゼ粉末製剤、その製造方法及び使用 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US8921081B2 (ja) |
| EP (1) | EP2141229B1 (ja) |
| JP (1) | JP5145609B2 (ja) |
| KR (1) | KR101213082B1 (ja) |
| CN (2) | CN101675159A (ja) |
| AU (1) | AU2008227690B2 (ja) |
| CA (1) | CA2680863C (ja) |
| DK (1) | DK2141229T3 (ja) |
| ES (1) | ES2537325T3 (ja) |
| MY (1) | MY151041A (ja) |
| RU (1) | RU2447148C2 (ja) |
| TW (1) | TWI438279B (ja) |
| WO (1) | WO2008114656A1 (ja) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010089967A1 (ja) * | 2009-02-04 | 2010-08-12 | 不二製油株式会社 | 乳化粉末リパーゼ製剤の製造方法 |
| WO2011068076A1 (ja) * | 2009-12-01 | 2011-06-09 | 日清オイリオグループ株式会社 | リパーゼ粉末製剤及びその使用 |
| JP2012017284A (ja) * | 2010-07-07 | 2012-01-26 | Adeka Corp | リパーゼ製剤及びその製造方法 |
| JP2013039109A (ja) * | 2011-08-19 | 2013-02-28 | Nisshin Oillio Group Ltd | リパーゼ活性の回復方法 |
| JP2013049803A (ja) * | 2011-08-31 | 2013-03-14 | Nisshin Oillio Group Ltd | エステル交換油脂の製造方法及びその装置 |
| JP2015216846A (ja) * | 2014-05-14 | 2015-12-07 | 長谷川香料株式会社 | 大豆食品の風味付与剤 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3622043B1 (en) * | 2017-05-08 | 2025-04-30 | Bunge Loders Croklaan B.V. | EMULSIFIERS |
| CN116179620B (zh) * | 2022-12-19 | 2025-06-17 | 河南工业大学 | 一种含有中链或短链脂肪酸的结构脂的生产方法 |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6098984A (ja) | 1983-09-05 | 1985-06-01 | ノボ ノルディスク アクティーゼルスカブ | 固定化リパ−ゼ調製品の製造方法 |
| JPS61202688A (ja) | 1985-02-27 | 1986-09-08 | ノボ ノルディスク アクティーゼルスカブ | 固定化リパーゼ調製品の製造方法 |
| JPS62269685A (ja) * | 1986-05-15 | 1987-11-24 | Showa Denko Kk | 酵素粒剤 |
| JPH01262795A (ja) | 1988-04-11 | 1989-10-19 | Kao Corp | 固定化酵素の製造方法 |
| JPH02138986A (ja) | 1987-12-22 | 1990-05-28 | Unilever Nv | 脂肪酸エステルの製造方法 |
| JPH0361485A (ja) | 1989-07-31 | 1991-03-18 | Ajinomoto Co Inc | 固定化リパーゼ酵素剤 |
| JP2668187B2 (ja) | 1993-09-17 | 1997-10-27 | 日清製油株式会社 | リパーゼ粉末を用いたエステル交換法 |
| JPH11246893A (ja) | 1998-03-04 | 1999-09-14 | Kao Corp | 酵素固形製剤の製造方法 |
| JP2000106873A (ja) | 1998-10-06 | 2000-04-18 | Nisshin Oil Mills Ltd:The | 熱安定性酵素およびその製造法 |
| JP3403202B2 (ja) | 1995-06-27 | 2003-05-06 | ユニリーバー・ナームローゼ・ベンノートシャープ | 固定された酵素及びトリグリセリド油の加工への使用 |
| WO2006030889A1 (ja) * | 2004-09-16 | 2006-03-23 | The Nisshin Oillio Group, Ltd. | リパーゼ粉末、その製造方法及びその使用 |
| JP2006325465A (ja) * | 2005-05-25 | 2006-12-07 | Nisshin Oillio Group Ltd | 官能基交換反応用リパーゼ含有組成物、その製造方法及びその使用 |
| JP2007068426A (ja) * | 2005-09-05 | 2007-03-22 | Nisshin Oillio Group Ltd | リパーゼ粉末製剤、その製造方法及び使用 |
| WO2008010543A1 (en) * | 2006-07-19 | 2008-01-24 | The Nisshin Oillio Group, Ltd. | Process for production of hard butter suitable for chocolate product |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3660571A (en) * | 1968-03-28 | 1972-05-02 | Takeda Chemical Industries Ltd | Disease control composition for silkworms |
| JPH0640822B2 (ja) | 1985-12-13 | 1994-06-01 | 旭電化工業株式会社 | 微生物リポプロテインリパ−ゼの製造方法 |
| JP3690951B2 (ja) * | 1999-12-22 | 2005-08-31 | 日清オイリオグループ株式会社 | 油脂のエステル交換反応方法 |
| RU2233325C1 (ru) * | 2003-01-08 | 2004-07-27 | Государственное образовательное учреждение Воронежская государственная технологическая академия | Способ получения комплексного ферментного препарата липазы и липоксигеназы |
| WO2004105495A2 (en) * | 2003-05-29 | 2004-12-09 | Micro Baked Close Corporation | Bread premix and bread produced utilizing same |
| MY142014A (en) * | 2004-04-08 | 2010-08-16 | Nisshin Oillio Group Ltd | A lipase powder, methods for producing the same and use thereof |
| TW200538464A (en) | 2004-04-08 | 2005-12-01 | Nisshin Oillio Group Ltd | A 1,3-specific lipase powder, methods for producing the same and use thereof |
| JP4394598B2 (ja) * | 2004-08-24 | 2010-01-06 | 日清オイリオグループ株式会社 | リパーゼ粉末組成物及びそれを用いたエステル化物の製造方法 |
| WO2006098399A1 (ja) | 2005-03-16 | 2006-09-21 | Fuji Oil Company, Limited | 大豆パフの製造法 |
-
2008
- 2008-03-11 TW TW097108453A patent/TWI438279B/zh active
- 2008-03-12 CA CA2680863A patent/CA2680863C/en not_active Expired - Fee Related
- 2008-03-12 ES ES08721864.0T patent/ES2537325T3/es active Active
- 2008-03-12 RU RU2009138225/10A patent/RU2447148C2/ru not_active IP Right Cessation
- 2008-03-12 AU AU2008227690A patent/AU2008227690B2/en not_active Ceased
- 2008-03-12 MY MYPI20093853A patent/MY151041A/en unknown
- 2008-03-12 CN CN200880014725A patent/CN101675159A/zh active Pending
- 2008-03-12 DK DK08721864.0T patent/DK2141229T3/en active
- 2008-03-12 CN CN201510369736.0A patent/CN104962535B/zh active Active
- 2008-03-12 US US12/531,327 patent/US8921081B2/en active Active
- 2008-03-12 KR KR1020097019379A patent/KR101213082B1/ko active Active
- 2008-03-12 JP JP2009505150A patent/JP5145609B2/ja active Active
- 2008-03-12 WO PCT/JP2008/054448 patent/WO2008114656A1/ja not_active Ceased
- 2008-03-12 EP EP08721864.0A patent/EP2141229B1/en active Active
-
2014
- 2014-12-17 US US14/573,383 patent/US20150104839A1/en not_active Abandoned
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6098984A (ja) | 1983-09-05 | 1985-06-01 | ノボ ノルディスク アクティーゼルスカブ | 固定化リパ−ゼ調製品の製造方法 |
| JPS61202688A (ja) | 1985-02-27 | 1986-09-08 | ノボ ノルディスク アクティーゼルスカブ | 固定化リパーゼ調製品の製造方法 |
| JPS62269685A (ja) * | 1986-05-15 | 1987-11-24 | Showa Denko Kk | 酵素粒剤 |
| JPH02138986A (ja) | 1987-12-22 | 1990-05-28 | Unilever Nv | 脂肪酸エステルの製造方法 |
| JPH01262795A (ja) | 1988-04-11 | 1989-10-19 | Kao Corp | 固定化酵素の製造方法 |
| JPH0361485A (ja) | 1989-07-31 | 1991-03-18 | Ajinomoto Co Inc | 固定化リパーゼ酵素剤 |
| JP2668187B2 (ja) | 1993-09-17 | 1997-10-27 | 日清製油株式会社 | リパーゼ粉末を用いたエステル交換法 |
| JP3403202B2 (ja) | 1995-06-27 | 2003-05-06 | ユニリーバー・ナームローゼ・ベンノートシャープ | 固定された酵素及びトリグリセリド油の加工への使用 |
| JPH11246893A (ja) | 1998-03-04 | 1999-09-14 | Kao Corp | 酵素固形製剤の製造方法 |
| JP2000106873A (ja) | 1998-10-06 | 2000-04-18 | Nisshin Oil Mills Ltd:The | 熱安定性酵素およびその製造法 |
| WO2006030889A1 (ja) * | 2004-09-16 | 2006-03-23 | The Nisshin Oillio Group, Ltd. | リパーゼ粉末、その製造方法及びその使用 |
| JP2006325465A (ja) * | 2005-05-25 | 2006-12-07 | Nisshin Oillio Group Ltd | 官能基交換反応用リパーゼ含有組成物、その製造方法及びその使用 |
| JP2007068426A (ja) * | 2005-09-05 | 2007-03-22 | Nisshin Oillio Group Ltd | リパーゼ粉末製剤、その製造方法及び使用 |
| WO2008010543A1 (en) * | 2006-07-19 | 2008-01-24 | The Nisshin Oillio Group, Ltd. | Process for production of hard butter suitable for chocolate product |
Non-Patent Citations (1)
| Title |
|---|
| See also references of EP2141229A4 |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010089967A1 (ja) * | 2009-02-04 | 2010-08-12 | 不二製油株式会社 | 乳化粉末リパーゼ製剤の製造方法 |
| JP5585454B2 (ja) * | 2009-02-04 | 2014-09-10 | 不二製油株式会社 | 乳化粉末リパーゼ製剤の製造方法 |
| WO2011068076A1 (ja) * | 2009-12-01 | 2011-06-09 | 日清オイリオグループ株式会社 | リパーゼ粉末製剤及びその使用 |
| CN102575238A (zh) * | 2009-12-01 | 2012-07-11 | 日清奥利友集团株式会社 | 脂肪酶粉末制剂及其用途 |
| EP2508598A4 (en) * | 2009-12-01 | 2013-05-29 | Nisshin Oillio Group Ltd | PREPARATION OF LIPASE POWDER AND USE THEREOF |
| JP2012017284A (ja) * | 2010-07-07 | 2012-01-26 | Adeka Corp | リパーゼ製剤及びその製造方法 |
| JP2013039109A (ja) * | 2011-08-19 | 2013-02-28 | Nisshin Oillio Group Ltd | リパーゼ活性の回復方法 |
| WO2013027697A1 (ja) * | 2011-08-19 | 2013-02-28 | 日清オイリオグループ株式会社 | リパーゼ活性の回復方法 |
| JP2013049803A (ja) * | 2011-08-31 | 2013-03-14 | Nisshin Oillio Group Ltd | エステル交換油脂の製造方法及びその装置 |
| JP2015216846A (ja) * | 2014-05-14 | 2015-12-07 | 長谷川香料株式会社 | 大豆食品の風味付与剤 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2141229A1 (en) | 2010-01-06 |
| MY151041A (en) | 2014-03-31 |
| CA2680863C (en) | 2012-05-29 |
| RU2009138225A (ru) | 2011-04-27 |
| JPWO2008114656A1 (ja) | 2010-07-01 |
| TW200848513A (en) | 2008-12-16 |
| CN104962535A (zh) | 2015-10-07 |
| CA2680863A1 (en) | 2008-09-25 |
| AU2008227690B2 (en) | 2013-09-05 |
| AU2008227690A1 (en) | 2008-09-25 |
| EP2141229B1 (en) | 2015-05-13 |
| JP5145609B2 (ja) | 2013-02-20 |
| KR101213082B1 (ko) | 2012-12-17 |
| TWI438279B (zh) | 2014-05-21 |
| CN104962535B (zh) | 2018-11-02 |
| EP2141229A4 (en) | 2010-06-02 |
| US8921081B2 (en) | 2014-12-30 |
| US20150104839A1 (en) | 2015-04-16 |
| US20100112650A1 (en) | 2010-05-06 |
| ES2537325T3 (es) | 2015-06-05 |
| RU2447148C2 (ru) | 2012-04-10 |
| DK2141229T3 (en) | 2015-08-03 |
| KR20090122241A (ko) | 2009-11-26 |
| CN101675159A (zh) | 2010-03-17 |
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