WO2008129997A1 - 可溶化羊膜組成物及びその用途 - Google Patents

可溶化羊膜組成物及びその用途 Download PDF

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Publication number
WO2008129997A1
WO2008129997A1 PCT/JP2008/057427 JP2008057427W WO2008129997A1 WO 2008129997 A1 WO2008129997 A1 WO 2008129997A1 JP 2008057427 W JP2008057427 W JP 2008057427W WO 2008129997 A1 WO2008129997 A1 WO 2008129997A1
Authority
WO
WIPO (PCT)
Prior art keywords
amnion
composition
cell culture
solubilized
coated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/057427
Other languages
English (en)
French (fr)
Inventor
Norio Sakuragawa
Isao Kamo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SCHOOL JURIDICAL PERSON KITASATO GAKUEN
Original Assignee
SCHOOL JURIDICAL PERSON KITASATO GAKUEN
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SCHOOL JURIDICAL PERSON KITASATO GAKUEN filed Critical SCHOOL JURIDICAL PERSON KITASATO GAKUEN
Priority to EP08740506A priority Critical patent/EP2149601B1/en
Priority to JP2009510852A priority patent/JP5458279B2/ja
Publication of WO2008129997A1 publication Critical patent/WO2008129997A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662Stem cells
    • C12N5/0663Bone marrow mesenchymal stem cells (BM-MSC)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C12N2533/92Amnion; Decellularised dermis or mucosa

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

羊膜自体をコーティングした培養ディッシュよりも取り扱い性に優れ、より細胞培養に適した安価な羊膜組成物およびそれを用いたコーティング材、特に幹細胞などの再生医療に適した安全な細胞培養用コーティング材が開示されている。該羊膜組成物は、加熱下での酸処理により可溶化した羊膜の可溶化物を媒体中に含む。また、該コーティング材は、該羊膜組成物を基体上にコーティングし、乾燥して成る。これらにより、羊膜自体よりも取り扱い性に優れ、細胞の培養に適した安全で安価な材料を提供し、該材料を用いることで細胞の増殖能を高めることが可能である。
PCT/JP2008/057427 2007-04-17 2008-04-16 可溶化羊膜組成物及びその用途 Ceased WO2008129997A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08740506A EP2149601B1 (en) 2007-04-17 2008-04-16 Composition of solubilized amnion and use thereof
JP2009510852A JP5458279B2 (ja) 2007-04-17 2008-04-16 可溶化羊膜組成物及びその用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-108077 2007-04-17
JP2007108077 2007-04-17

Publications (1)

Publication Number Publication Date
WO2008129997A1 true WO2008129997A1 (ja) 2008-10-30

Family

ID=39875525

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057427 Ceased WO2008129997A1 (ja) 2007-04-17 2008-04-16 可溶化羊膜組成物及びその用途

Country Status (3)

Country Link
EP (1) EP2149601B1 (ja)
JP (1) JP5458279B2 (ja)
WO (1) WO2008129997A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150138157A (ko) * 2013-01-09 2015-12-09 아이에스이 프로페셔널 테스팅 앤드 컨설팅 서비시즈, 인코퍼레이티드 비-포유류 조직으로부터의 세포제거된 생체재료

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117298343A (zh) * 2022-06-24 2023-12-29 济南金泉生物科技有限公司 一种复合型脱细胞羊膜组织诱导再生活性材料与其制备方法及用途

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001161353A (ja) * 1999-12-09 2001-06-19 Japan Ophthalmic Consultants:Kk 移植用細胞片及びその作成方法
WO2004076631A2 (en) * 2003-02-21 2004-09-10 The Uab Research Foundation Biologically active native biomatrix composition

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5234535B2 (ja) * 2007-06-25 2013-07-10 株式会社ニデック 哺乳動物未成熟卵子の体外成熟培養用添加剤及びそれを用いた成熟卵子の作出方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001161353A (ja) * 1999-12-09 2001-06-19 Japan Ophthalmic Consultants:Kk 移植用細胞片及びその作成方法
WO2004076631A2 (en) * 2003-02-21 2004-09-10 The Uab Research Foundation Biologically active native biomatrix composition

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KOBAYASHI M. ET AL.: "Enhancement of mesenchymal cell proliferation by using solubilized amniotic membrane as scaffold", HUM. CELL, vol. 21, no. 1, 2008, pages A5 + ABSTR. NO. O-02, XP008123513 *
KUMAR T.R ET AL.: "Biocompatible collagen scaffolds from a human amniotic membrane: physicochemical and in vitro culture characteristics", J. BIOMATER. SCI. POLYMER EDN., vol. 14, no. 7, 2003, pages 689 - 706, XP009117131 *
MORIO UENO ET AL., PNAS, vol. 103, no. 25, 20 June 2006 (2006-06-20), pages 9554 - 9559
See also references of EP2149601A4 *
T. R. KUMAR ET AL., J. BIOMATER. SCI. POLYMER EDN., vol. 14, no. 7, 2003, pages 689 - 706

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150138157A (ko) * 2013-01-09 2015-12-09 아이에스이 프로페셔널 테스팅 앤드 컨설팅 서비시즈, 인코퍼레이티드 비-포유류 조직으로부터의 세포제거된 생체재료
JP2016504932A (ja) * 2013-01-09 2016-02-18 アイエスイー プロフェッショナル テスティング アンド コンサルティング サービシズ,インコーポレーテッドIse Professional Testing & Consulting Services,Inc. 非哺乳動物組織からの脱細胞化生体材料
JP2019076093A (ja) * 2013-01-09 2019-05-23 アイエスイー プロフェッショナル テスティング アンド コンサルティング サービシズ,インコーポレーテッドIse Professional Testing & Consulting Services,Inc. 非哺乳動物組織からの脱細胞化生体材料
US10617790B2 (en) 2013-01-09 2020-04-14 Ise Professional Testing & Consulting Services, Inc. Decellularized biomaterial from non-mammalian tissue
KR102284982B1 (ko) 2013-01-09 2021-08-03 넥스트젠 바이오로직스, 아이엔씨. 비-포유류 조직으로부터의 세포제거된 생체재료
US11660376B2 (en) 2013-01-09 2023-05-30 NeXtGen Biologies, Inc. Decellularized biomaterial from non-mammalian tissue
US12311079B2 (en) 2013-01-09 2025-05-27 Nextgen Biologics, Inc. Decellularized biomaterial from non-mammalian tissue

Also Published As

Publication number Publication date
EP2149601A1 (en) 2010-02-03
JP5458279B2 (ja) 2014-04-02
EP2149601A4 (en) 2010-05-05
EP2149601B1 (en) 2012-12-05
JPWO2008129997A1 (ja) 2010-07-22

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