WO2008149365A3 - Systems and methods for focusing particles - Google Patents

Systems and methods for focusing particles Download PDF

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Publication number
WO2008149365A3
WO2008149365A3 PCT/IL2008/000772 IL2008000772W WO2008149365A3 WO 2008149365 A3 WO2008149365 A3 WO 2008149365A3 IL 2008000772 W IL2008000772 W IL 2008000772W WO 2008149365 A3 WO2008149365 A3 WO 2008149365A3
Authority
WO
WIPO (PCT)
Prior art keywords
suspension
particles
flowing
systems
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2008/000772
Other languages
French (fr)
Other versions
WO2008149365A2 (en
Inventor
Alexander Leshansky
Avishay Bransky
Korin Natanel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technion Research and Development Foundation Ltd
Original Assignee
Technion Research and Development Foundation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technion Research and Development Foundation Ltd filed Critical Technion Research and Development Foundation Ltd
Priority to US12/663,492 priority Critical patent/US8642288B2/en
Priority to CN2008801016866A priority patent/CN101772697B/en
Priority to JP2010510958A priority patent/JP5337151B2/en
Priority to EP08763530.6A priority patent/EP2153199B1/en
Publication of WO2008149365A2 publication Critical patent/WO2008149365A2/en
Publication of WO2008149365A3 publication Critical patent/WO2008149365A3/en
Anticipated expiration legal-status Critical
Priority to US14/139,490 priority patent/US9470679B2/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N15/1409—Handling samples, e.g. injecting samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2203/00—Investigating strength properties of solid materials by application of mechanical stress
    • G01N2203/0058—Kind of property studied
    • G01N2203/0092—Visco-elasticity, solidification, curing, cross-linking degree, vulcanisation or strength properties of semi-solid materials
    • G01N2203/0094—Visco-elasticity

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided is a method of focusing particles. The method includes: providing a suspension of the particles in a suspending medium; and flowing the suspension along a channel, such that the flowing suspension occupies a certain volume that has at least one cross-sectional dimension smaller than 100µm. The suspending medium has such viscoelastic properties, that flowing the suspension in the channel directs at least some of the particles towards a focus region, enclosed in said certain volume.
PCT/IL2008/000772 2007-06-07 2008-06-05 Systems and methods for focusing particles Ceased WO2008149365A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/663,492 US8642288B2 (en) 2007-06-07 2008-06-05 Methods for viscoelastic focusing of particles
CN2008801016866A CN101772697B (en) 2007-06-07 2008-06-05 Systems and methods for aggregating particles
JP2010510958A JP5337151B2 (en) 2007-06-07 2008-06-05 System and method for focusing particles
EP08763530.6A EP2153199B1 (en) 2007-06-07 2008-06-05 System and method for focusing particles
US14/139,490 US9470679B2 (en) 2007-06-07 2013-12-23 Systems and methods for focusing particles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92499807P 2007-06-07 2007-06-07
US60/924,998 2007-06-07

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/663,492 A-371-Of-International US8642288B2 (en) 2007-06-07 2008-06-05 Methods for viscoelastic focusing of particles
US14/139,490 Division US9470679B2 (en) 2007-06-07 2013-12-23 Systems and methods for focusing particles

Publications (2)

Publication Number Publication Date
WO2008149365A2 WO2008149365A2 (en) 2008-12-11
WO2008149365A3 true WO2008149365A3 (en) 2009-03-05

Family

ID=39800715

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000772 Ceased WO2008149365A2 (en) 2007-06-07 2008-06-05 Systems and methods for focusing particles

Country Status (5)

Country Link
US (2) US8642288B2 (en)
EP (1) EP2153199B1 (en)
JP (2) JP5337151B2 (en)
CN (2) CN103543093B (en)
WO (1) WO2008149365A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104155210B (en) * 2008-10-02 2017-08-22 彼克斯赛尔医疗科技有限公司 Optical imagery based on viscoelastic focusing
CN103608110B (en) 2011-03-09 2017-06-06 彼克斯赛尔医疗科技有限公司 Disposable cassette for preparing sample fluid containing cells for analysis
GB201113309D0 (en) * 2011-08-02 2011-09-14 Izon Science Ltd Characterisation of particles
IN2015MN00110A (en) * 2012-06-22 2015-10-16 Malvern Instr Ltd
US20150362421A1 (en) * 2012-06-22 2015-12-17 Malvern Instruments Limited Particle characterization
US10509976B2 (en) 2012-06-22 2019-12-17 Malvern Panalytical Limited Heterogeneous fluid sample characterization
US9669403B2 (en) * 2012-07-25 2017-06-06 Sony Corporation Microparticle measurement device and liquid delivery method in microparticle measurement device
US9372144B2 (en) * 2013-10-01 2016-06-21 Owl biomedical, Inc. Particle manipulation system with out-of-plane channel
KR102095617B1 (en) 2013-03-15 2020-03-31 아이리스 인터내셔널 인크. Autofocus systems and methods for particle analysis in blood samples
US9316635B2 (en) 2013-03-15 2016-04-19 Iris International, Inc. Sheath fluid systems and methods for particle analysis in blood samples
CN105705934B (en) * 2013-09-03 2019-01-08 Izon科技有限公司 Method and apparatus for determining the charge of measurement particle
WO2015116990A1 (en) * 2014-01-30 2015-08-06 The General Hospital Corporation Inertio-elastic focusing of particles in microchannels
EP2907574A1 (en) 2014-02-13 2015-08-19 OWL Biobedical, Inc. Microfluidic system with viscoelastic fluid
EP4115978A1 (en) 2015-01-14 2023-01-11 Pixcell Medical Technologies Ltd. Disposable cartridge for sample fluid analysis
WO2016165945A1 (en) * 2015-04-17 2016-10-20 Koninklijke Philips N.V. Dust processing
KR101855490B1 (en) * 2016-01-22 2018-05-08 한국과학기술원 Method For Separating And Washing Of Microparticles Via A Stratified Coflow Of Non-Newtonian And Newtonian Fluids
JP6134402B1 (en) * 2016-01-29 2017-05-24 シスメックス株式会社 Biological sample imaging apparatus and biological sample imaging method
CN106248528A (en) * 2016-09-27 2016-12-21 中国石油天然气股份有限公司 A Method for Evaluating the Elasticity of Polymers
EP3631415B1 (en) * 2017-06-02 2026-03-25 The General Hospital Corporation Oscillatory focusing of particles in channels
CN108627448A (en) * 2018-06-05 2018-10-09 江苏卓微生物科技有限公司 The method of counting micro particles
US11185861B2 (en) 2018-06-13 2021-11-30 International Business Machines Corporation Multistage deterministic lateral displacement device for particle separation
EP4222276A1 (en) * 2020-09-29 2023-08-09 1087 Systems, Inc. Graded bioprocess monitoring and fluid self-modulation
EP4083607A1 (en) 2021-04-26 2022-11-02 ETH Zurich Method and microfluidic device for studying cell deformations
JP7152622B1 (en) * 2022-03-30 2022-10-12 ポーラ化成工業株式会社 Bubble concentration measurement method for ultra-fine bubbles

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040070757A1 (en) * 2000-12-29 2004-04-15 Moore Richard Channing High viscosity sheath reagent for flow cytometry

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS576338A (en) * 1980-06-12 1982-01-13 Kyoto Daiichi Kagaku:Kk Method and device for measuring degree of flocculation of finely divided particles quantitatively
FR2621123B1 (en) * 1987-09-30 1989-12-01 Commissariat Energie Atomique METHOD FOR MANUFACTURING A DEVICE FOR OPTICAL ANALYSIS OF A MICROPARTICLE STREAM AND APPLICATION TO THE MANUFACTURE OF A CYTOFLUORIMETER
JP2813988B2 (en) * 1989-08-08 1998-10-22 東亜医用電子株式会社 Erythrocyte deformability measurement method and apparatus
JP3467310B2 (en) * 1994-04-21 2003-11-17 シスメックス株式会社 Leukocyte analysis reagent and leukocyte classification method
FR2734637B1 (en) * 1995-05-24 1997-08-14 Abx Sa DEVICE FOR OPTICAL INSPECTION OF A FLUID, PARTICULARLY FOR HEMATOLOGICAL ANALYSIS
JPH09126989A (en) * 1995-11-07 1997-05-16 Hitachi Ltd Flow-type particle image analyzer
US5808737A (en) * 1996-02-29 1998-09-15 Sienna Biotech, Inc. Pre-analysis chamber for a flow particle analyzer
JPH09318523A (en) * 1996-05-29 1997-12-12 Toa Medical Electronics Co Ltd Parameter analysis method and apparatus using the same
US6723290B1 (en) * 1998-03-07 2004-04-20 Levine Robert A Container for holding biologic fluid for analysis
IL148088A0 (en) * 1999-08-13 2002-09-12 Us Genomics Inc Methods and apparatuses for stretching polymers
JP2004500562A (en) * 1999-12-29 2004-01-08 ユニオン　バイオメトリカ　インコーポレイテッド High viscosity sheath reagent for flow cytometry
US20030027225A1 (en) * 2001-07-13 2003-02-06 Caliper Technologies Corp. Microfluidic devices and systems for separating components of a mixture
DK2184346T3 (en) * 2001-09-06 2017-06-26 Rapid Micro Biosystems Inc Rapid detection of replicating cells
US6746872B2 (en) * 2002-01-16 2004-06-08 Lifescan, Inc. Control compositions and methods of use for coagulation tests
JP2003302330A (en) * 2002-04-12 2003-10-24 Asahi Kasei Corp Flat plate flow cell device
US6794671B2 (en) * 2002-07-17 2004-09-21 Particle Sizing Systems, Inc. Sensors and methods for high-sensitivity optical particle counting and sizing
US7115230B2 (en) * 2003-06-26 2006-10-03 Intel Corporation Hydrodynamic focusing devices
AU2005220150A1 (en) * 2004-02-13 2005-09-15 The University Of North Carolina At Chapel Hill Functional materials and novel methods for the fabrication of microfluidic devices
JP4528664B2 (en) * 2004-04-22 2010-08-18 興和株式会社 Fluorescent particle counter
ITBO20040420A1 (en) * 2004-07-07 2004-10-07 Type S R L METAL CUTTING AND FORMING MACHINE
US7811438B2 (en) * 2004-12-08 2010-10-12 Palo Alto Research Center Incorporated Bio-enrichment device to enhance sample collection and detection
US8186913B2 (en) * 2007-04-16 2012-05-29 The General Hospital Corporation Systems and methods for particle focusing in microchannels

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040070757A1 (en) * 2000-12-29 2004-04-15 Moore Richard Channing High viscosity sheath reagent for flow cytometry

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRANSKY AVISHAY ET AL: "The rheologic properties of erythrocytes: a study using an automated rheoscope", RHEOLOGICA ACTA, SPRINGER-VERLAG, DE, vol. 46, no. 5, 20 April 2007 (2007-04-20), pages 621 - 627, XP019516525, ISSN: 1435-1528 *
FAIVRE MAGALIE ET AL: "Geometrical focusing of cells in a microfluidic device: an approach to separate blood plasma.", 2006, BIORHEOLOGY 2006, VOL. 43, NR. 2, PAGE(S) 147 - 159, ISSN: 0006-355X, XP002499735 *
ZHIGANG WU ET AL: "Rapid Mixing Using Two-Phase Hydraulic Focusing in Microchannels", BIOMEDICAL MICRODEVICES, KLUWER ACADEMIC PUBLISHERS, BO, vol. 7, no. 1, 1 March 2005 (2005-03-01), pages 13 - 20, XP019205080, ISSN: 1572-8781 *

Also Published As

Publication number Publication date
JP5901582B2 (en) 2016-04-13
WO2008149365A2 (en) 2008-12-11
CN101772697A (en) 2010-07-07
JP5337151B2 (en) 2013-11-06
US20100178666A1 (en) 2010-07-15
EP2153199A2 (en) 2010-02-17
US20140113358A1 (en) 2014-04-24
JP2013217946A (en) 2013-10-24
EP2153199B1 (en) 2016-10-19
CN103543093B (en) 2016-08-10
JP2010529452A (en) 2010-08-26
CN103543093A (en) 2014-01-29
CN101772697B (en) 2013-08-21
US8642288B2 (en) 2014-02-04
US9470679B2 (en) 2016-10-18

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