WO2009011426A1 - γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法 - Google Patents

γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法 Download PDF

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Publication number
WO2009011426A1
WO2009011426A1 PCT/JP2008/063037 JP2008063037W WO2009011426A1 WO 2009011426 A1 WO2009011426 A1 WO 2009011426A1 JP 2008063037 W JP2008063037 W JP 2008063037W WO 2009011426 A1 WO2009011426 A1 WO 2009011426A1
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met
secretase
screening method
method utilizing
novel substrate
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PCT/JP2008/063037
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English (en)
French (fr)
Inventor
Eiji Inoue
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Eisai R&D Management Co Ltd
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Eisai R&D Management Co Ltd
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Priority to EP08791346A priority Critical patent/EP2177623A4/en
Priority to JP2009523688A priority patent/JPWO2009011426A1/ja
Publication of WO2009011426A1 publication Critical patent/WO2009011426A1/ja
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　本発明は、γ-セクレターゼによるc-Metのプロセシングに影響を与える化合物をスクリーニングする方法であって、以下のステップ： 　　(i)候補化合物の存在下および非存在下で、γ-セクレターゼまたはその生物学的に活性な断片を含む第１の生物学的組成物と、c-Metを含む第２の生物学的組成物とを接触させ； 　　(ii)当該候補化合物の存在下および非存在下における当該c-Metの開裂をそれぞれ測定し； 　　(iii)γ-セクレターゼによる当該c-Metの開裂に影響を与える候補化合物を選択し；そして 　　(iv)前記(iii)で得られた候補化合物を、γ-セクレターゼによるc-Metのプロセシングに影響を与える化合物であると同定する、 ことを含む、上記方法。
PCT/JP2008/063037 2007-07-19 2008-07-18 γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法 Ceased WO2009011426A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08791346A EP2177623A4 (en) 2007-07-19 2008-07-18 SCREENING METHOD USING C-MET, A NEW SUBSTRATE FOR GAMMA SECRETASE
JP2009523688A JPWO2009011426A1 (ja) 2007-07-19 2008-07-18 γ−セクレターゼの新規基質ｃ−Ｍｅｔを利用したスクリーニング方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US95062107P 2007-07-19 2007-07-19
US60/950,621 2007-07-19
US95149307P 2007-07-24 2007-07-24
US60/951,493 2007-07-24

Publications (1)

Publication Number Publication Date
WO2009011426A1 true WO2009011426A1 (ja) 2009-01-22

Family

ID=40259752

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063037 Ceased WO2009011426A1 (ja) 2007-07-19 2008-07-18 γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法

Country Status (4)

Country Link
US (1) US20090191580A1 (ja)
EP (1) EP2177623A4 (ja)
JP (1) JPWO2009011426A1 (ja)
WO (1) WO2009011426A1 (ja)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2166110B1 (en) * 2007-06-08 2012-02-22 Eisai R&D Management Co., Ltd. Screening method utilizing novel substrate epha4 for gamma-secretase
WO2009017234A1 (ja) * 2007-08-01 2009-02-05 Eisai R & D Management Co., Ltd. γ-セクレターゼの新規基質c-Retを利用したスクリーニング方法
US8530181B2 (en) * 2007-11-15 2013-09-10 Eisai R&D Management Co., Ltd. Method of screening for compounds which affect the cleavage of EphA7 byγ-secretase
US7892769B2 (en) * 2007-11-30 2011-02-22 Eisai R&D Management Co., Ltd. Processing of EphA4 polypeptide by γ-secretase activity
EP3165538A1 (en) 2010-11-03 2017-05-10 Argen-X Nv Anti c-met antibodies
WO2012081502A1 (ja) 2010-12-17 2012-06-21 エーザイ・アール・アンド・ディー・マネジメント株式会社 ゼラチナーゼによるEphA4の切断反応を指標としたスクリーニング方法
US9784751B2 (en) 2011-04-25 2017-10-10 Eisai R&D Management Co., Ltd. Method for detecting neurological disease associated with cognitive impairment by measuring EphA4 extracellular domain
EP3995582B1 (en) 2019-07-01 2026-04-22 Eisai R&D Management Co., Ltd. Anti-epha4 antibody

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Publication number Priority date Publication date Assignee Title
US20020068361A1 (en) * 1997-04-08 2002-06-06 Douglas Clary Methods of evaluating specific cellular functions of receptor protein tyrosine kinases in a ligand independent manner
US20070026409A1 (en) * 2001-08-14 2007-02-01 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
AU2002324700A1 (en) * 2001-08-14 2003-03-03 Bayer Ag Nucleic acid and amino acid sequences involved in pain
US20060241074A1 (en) * 2001-08-14 2006-10-26 The General Hospital Corporation Methods for treatment of pain
JP2004000060A (ja) * 2002-05-31 2004-01-08 Otsuka Pharmaceut Co Ltd アミロイドβ産生に影響を与える化合物のスクリーニング方法
EP1662259A1 (en) * 2004-11-25 2006-05-31 Cellzome Ag Use of Eph receptor inhibitors for the treatment of neurodegenerative diseases
EP1947193A1 (en) * 2007-01-17 2008-07-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Screening method for anti-diabetic compounds
EP2166110B1 (en) * 2007-06-08 2012-02-22 Eisai R&D Management Co., Ltd. Screening method utilizing novel substrate epha4 for gamma-secretase
EP2183598A1 (en) * 2007-07-13 2010-05-12 Elan Pharmaceuticals Inc. Compositions and methods for identifying substrate specificity of inhibitors of gamma secretase
WO2009017234A1 (ja) * 2007-08-01 2009-02-05 Eisai R & D Management Co., Ltd. γ-セクレターゼの新規基質c-Retを利用したスクリーニング方法
US20090163594A1 (en) * 2007-10-31 2009-06-25 Elan Pharmaceuticals, Inc. Triple Assay System for Identifying Substrate Selectivity of Gamma Secretase Inhibitors
US8530181B2 (en) * 2007-11-15 2013-09-10 Eisai R&D Management Co., Ltd. Method of screening for compounds which affect the cleavage of EphA7 byγ-secretase
US7892769B2 (en) * 2007-11-30 2011-02-22 Eisai R&D Management Co., Ltd. Processing of EphA4 polypeptide by γ-secretase activity
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Non-Patent Citations (10)

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Title
AKIMOTO M, BABA A, IKEDA-MATSUO Y, YAMADA MK, ITAMURA R, NISHIYAMA N, IKEGAYA Y, MATSUKI N.: "Hepatocyte growth factor as an enhancer of nmda currents and synaptic plasticity in the hippocampus", NEUROSCIENCE, vol. 128, no. 1, 2004, pages 155 - 62
GEORGAKOPOULOS A. ET AL.: "Metalloproteinase/Presenilin1 processing of ephrinB regulates EphB-induced Src phosphorylation and signaling", EMBO J., vol. 25, March 2006 (2006-03-01), pages 1242 - 1252, XP008129422 *
KOPAN R. ET AL.: "Gamma-secretase: proteasome of the membrane?", NAT. REV. MOL. CELL BIOL., vol. 5, June 2004 (2004-06-01), pages 499 - 504, XP008061885 *
LANDMAN ET AL., CYTOKINE & GROWTH FACTOR REVIEW, vol. 15, 2004, pages 337 - 351
NATH D. ET AL.: "Shedding of c-Met is regulated by crosstalk between a G-protein coupled receptor and the EGF receptor and is mediated by a TIMP-3 sensitive metalloproteinase", J. CELL SCI., vol. 114, March 2001 (2001-03-01), pages 1213 - 1220, XP008129432 *
POZNER-MOULIS ET AL., CANCER RESEARCH, vol. 66, 2006, pages 7976 - 7982
SAURA CA, CHOI SY, BEGLOPOULOS V, MALKANI S, ZHANG D, SHANKARANARAYANA RAO BS, CHATTAGI S, KELLEHER RJ 3RD, KANDEL ER, DUFF K: "Loss of presenilin function causes impairments of memory and synaptic plasticity followed by age-dependent neurodegeneration", NEURON, vol. 42, no. 1, 8 April 2004 (2004-04-08), pages 23 - 36
See also references of EP2177623A4
TYNDALL SJ, WALIKONIS RS.: "The receptor tyrosine kinase Met and its ligand hepatocyte growth factor are clustered at excitatory synapses and can enhance clustering of synaptic proteins", CELL CYCLE, vol. 5, no. 14, 17 July 2006 (2006-07-17), pages 1560 - 8
VIDAL G.A. ET AL.: "Presenilin-dependent gamma-secretase processing regulates multiple ERBB4/HER4 activities", J. BIOL. CHEM., vol. 280, no. 20, May 2005 (2005-05-01), pages 19777 - 19783, XP008129417 *

Also Published As

Publication number Publication date
EP2177623A4 (en) 2010-12-29
EP2177623A1 (en) 2010-04-21
US20090191580A1 (en) 2009-07-30
JPWO2009011426A1 (ja) 2010-09-24

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