WO2009012428A3 - Differentiation of human embryonic stem cells - Google Patents

Differentiation of human embryonic stem cells Download PDF

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Publication number
WO2009012428A3
WO2009012428A3 PCT/US2008/070418 US2008070418W WO2009012428A3 WO 2009012428 A3 WO2009012428 A3 WO 2009012428A3 US 2008070418 W US2008070418 W US 2008070418W WO 2009012428 A3 WO2009012428 A3 WO 2009012428A3
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WO
WIPO (PCT)
Prior art keywords
differentiation
stem cells
embryonic stem
human embryonic
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/070418
Other languages
French (fr)
Other versions
WO2009012428A2 (en
Inventor
Alireza Rezania
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LifeScan Inc
Original Assignee
LifeScan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP21178371.7A priority Critical patent/EP3957716A1/en
Priority to HK10109459.3A priority patent/HK1142926B/en
Priority to MX2010000746A priority patent/MX2010000746A/en
Priority to BRPI0814425-7A2A priority patent/BRPI0814425A2/en
Priority to EP08796270.0A priority patent/EP2185695B1/en
Priority to CA2693156A priority patent/CA2693156C/en
Priority to ES08796270.0T priority patent/ES2537578T3/en
Priority to EP12172814.1A priority patent/EP2562248B1/en
Priority to CN200880107548A priority patent/CN101861386A/en
Application filed by LifeScan Inc filed Critical LifeScan Inc
Priority to US12/669,289 priority patent/US20100255580A1/en
Priority to JP2010517176A priority patent/JP5738591B2/en
Priority to PL08796270T priority patent/PL2185695T3/en
Publication of WO2009012428A2 publication Critical patent/WO2009012428A2/en
Publication of WO2009012428A3 publication Critical patent/WO2009012428A3/en
Anticipated expiration legal-status Critical
Priority to ZA2010/01158A priority patent/ZA201001158B/en
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Diabetes (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Emergency Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides an improved method for the formation of pancreatic endoderm, pancreatic hormone expressing cells and pancreatic hormone secreting cells. The present invention also provides methods to promote the differentiation of pluripotent stem cells without the use of a feeder cell layer.
PCT/US2008/070418 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells Ceased WO2009012428A2 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
CN200880107548A CN101861386A (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
MX2010000746A MX2010000746A (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells.
BRPI0814425-7A2A BRPI0814425A2 (en) 2007-07-18 2008-07-18 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS
EP08796270.0A EP2185695B1 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
CA2693156A CA2693156C (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
ES08796270.0T ES2537578T3 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
EP12172814.1A EP2562248B1 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
EP21178371.7A EP3957716A1 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
US12/669,289 US20100255580A1 (en) 2007-07-18 2008-07-18 Differentiation of Human Embryonic Stem Cells
HK10109459.3A HK1142926B (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
JP2010517176A JP5738591B2 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
PL08796270T PL2185695T3 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells
ZA2010/01158A ZA201001158B (en) 2007-07-18 2010-02-17 Diferentiation of human embryonic stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77931107A 2007-07-18 2007-07-18
US11/779,311 2007-07-18

Publications (2)

Publication Number Publication Date
WO2009012428A2 WO2009012428A2 (en) 2009-01-22
WO2009012428A3 true WO2009012428A3 (en) 2009-04-02

Family

ID=40260391

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/070418 Ceased WO2009012428A2 (en) 2007-07-18 2008-07-18 Differentiation of human embryonic stem cells

Country Status (14)

Country Link
US (1) US20100255580A1 (en)
EP (3) EP3957716A1 (en)
JP (1) JP5738591B2 (en)
KR (1) KR101555824B1 (en)
CN (2) CN101861386A (en)
BR (1) BRPI0814425A2 (en)
CA (1) CA2693156C (en)
DK (1) DK2562248T3 (en)
ES (1) ES2537578T3 (en)
MX (1) MX2010000746A (en)
PL (1) PL2185695T3 (en)
RU (1) RU2465323C2 (en)
WO (1) WO2009012428A2 (en)
ZA (1) ZA201001158B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
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US11981929B2 (en) 2014-05-16 2024-05-14 Janssen Biotech, Inc. Use of small molecules to enhance MAFA expression in pancreatic endocrine cells

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US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
JP2009500297A (en) 2005-06-08 2009-01-08 セントカー・インコーポレーテツド Cell therapy for ocular degeneration
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
DK2185693T3 (en) 2007-07-31 2019-09-23 Lifescan Inc DIFFERENTIZING HUMAN EMBRYONIC STEM CELLS
CA2954431C (en) 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
SG154367A1 (en) * 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
JP5733986B2 (en) 2008-02-21 2015-06-10 ヤンセン バイオテツク，インコーポレーテツド Methods, surface modified plates, and compositions for cell attachment, culture and detachment
US8623648B2 (en) * 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US7939322B2 (en) * 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
EP2942392B1 (en) 2008-06-30 2018-10-03 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
EP2350265B1 (en) * 2008-10-31 2019-04-17 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
US9234178B2 (en) 2008-10-31 2016-01-12 Janssen Biotech, Inc. Differentiation of human pluripotent stem cells
KR101687344B1 (en) 2008-11-20 2016-12-16 얀센 바이오테크 인코포레이티드 Methods and compositions for cell attachment and cultivation on planar substrates
KR101774546B1 (en) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 Pluripotent stem cell culture on micro-carriers
JP5694946B2 (en) * 2008-12-03 2015-04-01 インターナショナル ステム セル コーポレイション Method for obtaining differentiated cells from stem cells
EP2393917B1 (en) 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells.
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
US20120135519A1 (en) * 2009-05-29 2012-05-31 Cellartis Ab INDUCED DERIVATION OF SPECIFIC ENDODERM FROM hPS CELL-DERIVED DEFINITIVE ENDODERM
CN103952372B (en) 2009-07-20 2016-10-05 詹森生物科技公司 The differentiation of human embryo stem cell
US8785184B2 (en) * 2009-07-20 2014-07-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN102482643B (en) 2009-07-20 2016-06-29 詹森生物科技公司 The differentiation of human embryo stem cell
SG10201408552YA (en) 2009-12-23 2015-02-27 Janssen Biotech Inc Differentiation of human embryonic stem cells
CN102712902B (en) 2009-12-23 2019-01-08 詹森生物科技公司 Differentiation of Human Embryonic Stem Cells
CA2785966C (en) * 2009-12-29 2020-10-27 Takeda Pharmaceutical Company Limited Method for manufacturing pancreatic-hormone-producing cells
PH12012501686A1 (en) 2010-03-01 2020-10-19 Janssen Biotech Inc Methods for purifying cells derived from pluripotent stem cells
US9394522B2 (en) 2010-03-22 2016-07-19 Takara Bio Europe Ab Directed differentiation and maturation of pluripotent cells into hepatocyte like cells by modulation of Wnt-signalling pathway
RU2587634C2 (en) 2010-05-12 2016-06-20 Янссен Байотек, Инк. Differentiation of human embryo stem cells
KR101877077B1 (en) * 2010-08-09 2018-07-10 다케다 야쿠힌 고교 가부시키가이샤 Method of producing pancreatic hormone-producing cells
GB201014169D0 (en) 2010-08-25 2010-10-06 Cambridge Entpr Ltd In vitro hepatic differentiation
PL2853589T3 (en) * 2010-08-31 2018-05-30 Janssen Biotech, Inc Differentiation of human embryonic stem cells
EP3372672A1 (en) * 2010-08-31 2018-09-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN103154237B (en) 2010-08-31 2016-03-16 詹森生物科技公司 The differentiation of multipotential stem cell
EP2652125B1 (en) * 2010-12-15 2017-04-26 Kadimastem Ltd. Insulin producing cells derived from pluripotent stem cells
EP2723852B1 (en) * 2011-06-21 2021-11-17 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
CA2840192A1 (en) 2011-06-23 2012-12-27 The Children's Hospital Of Philadelphia Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof
FI2794857T3 (en) 2011-12-22 2026-03-25 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
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BR112014027783A2 (en) * 2012-05-07 2017-06-27 Janssen Biotech Inc differentiation of human embryonic stem cells into pancreatic endoderm
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