WO2009013160A3 - Butanol production in a eukaryotic cell - Google Patents

Butanol production in a eukaryotic cell Download PDF

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Publication number
WO2009013160A3
WO2009013160A3 PCT/EP2008/059120 EP2008059120W WO2009013160A3 WO 2009013160 A3 WO2009013160 A3 WO 2009013160A3 EP 2008059120 W EP2008059120 W EP 2008059120W WO 2009013160 A3 WO2009013160 A3 WO 2009013160A3
Authority
WO
WIPO (PCT)
Prior art keywords
butanol
ethanol
eukaryotic cell
water mixture
separating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2008/059120
Other languages
French (fr)
Other versions
WO2009013160A2 (en
Inventor
Einte-Karst Dijk
Lourina Madeleine Raamsdonk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Priority to US12/669,251 priority Critical patent/US20100205857A1/en
Priority to CN2008801004375A priority patent/CN101970673A/en
Priority to BRPI0814640-3A2A priority patent/BRPI0814640A2/en
Priority to EP08786098A priority patent/EP2171075A2/en
Publication of WO2009013160A2 publication Critical patent/WO2009013160A2/en
Publication of WO2009013160A3 publication Critical patent/WO2009013160A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D3/00—Distillation or related exchange processes in which liquids are contacted with gaseous media, e.g. stripping
    • B01D3/001—Processes specially adapted for distillation or rectification of fermented solutions
    • B01D3/002—Processes specially adapted for distillation or rectification of fermented solutions by continuous methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to a eukaryotic cell capable of producing butanol and ethanol at a ratio butanol: ethanol of between 1:2 to 1:100. The present invention further relates to a process for the preparation of butanol and ethanol comprising fermenting a eukaryotic cell in a suitable fermentation broth, wherein butanol and ethanol are produced at a ratio butanol: hethanol of between 1:2 to 1:100 and a process for the recovery of butanol and ethanol from an aqueous solution comprising butanol and ethanol wherein the ratio butanol: ethanol is between 1:2 to 1:100 comprising, separating an ethanol/butanol/water mixture from the aqueous solution; separating an ethanol/water mixture from the ethanol/butanol/water mixture; separating a butanol/water mixture from the ethanol/butanol/water mixture; and recovering of butanol and ethanol.
PCT/EP2008/059120 2007-07-23 2008-07-11 Butanol production in a eukaryotic cell Ceased WO2009013160A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/669,251 US20100205857A1 (en) 2007-07-23 2008-07-11 Butanol production in a eukaryotic cell
CN2008801004375A CN101970673A (en) 2007-07-23 2008-07-11 Butanol Production in Eukaryotic Cells
BRPI0814640-3A2A BRPI0814640A2 (en) 2007-07-23 2008-07-11 BUTANOL AND ETHANOL PREPARATION PROCESS
EP08786098A EP2171075A2 (en) 2007-07-23 2008-07-11 Butanol production in a eukaryotic cell

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
US93503107P 2007-07-23 2007-07-23
EP07112956 2007-07-23
US60/935,031 2007-07-23
EP07112956.3 2007-07-23
EP07123976 2007-12-21
EP07123976.8 2007-12-21
US6411808P 2008-02-19 2008-02-19
US6412008P 2008-02-19 2008-02-19
EP08101748.5 2008-02-19
EP08101747.7 2008-02-19
EP08101748 2008-02-19
US61/064,118 2008-02-19
US61/064,120 2008-02-19
EP08101747 2008-02-19
US4870008P 2008-04-29 2008-04-29
EP08155396.8 2008-04-29
US61/048,700 2008-04-29
EP08155396 2008-04-29

Publications (2)

Publication Number Publication Date
WO2009013160A2 WO2009013160A2 (en) 2009-01-29
WO2009013160A3 true WO2009013160A3 (en) 2009-04-02

Family

ID=39717707

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/059120 Ceased WO2009013160A2 (en) 2007-07-23 2008-07-11 Butanol production in a eukaryotic cell

Country Status (5)

Country Link
US (1) US20100205857A1 (en)
EP (1) EP2171075A2 (en)
CN (1) CN101970673A (en)
BR (1) BRPI0814640A2 (en)
WO (1) WO2009013160A2 (en)

Families Citing this family (30)

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WO2009013159A2 (en) * 2007-07-23 2009-01-29 Dsm Ip Assets B.V. Acetyl-coa producing enzymes in yeast
DE102008004253B4 (en) * 2008-01-14 2011-07-28 Butalco Gmbh Increased production of acetyl coenzyme A
CA2712779C (en) 2008-01-22 2021-03-16 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
DK2262901T3 (en) 2008-03-05 2019-01-21 Genomatica Inc ORGANISMS PRODUCING PRIMARY ALCOHOL
ES2656790T3 (en) 2008-03-27 2018-02-28 Genomatica, Inc. Microorganisms for the production of adipic acid and other compounds
WO2009135074A2 (en) 2008-05-01 2009-11-05 Genomatica, Inc. Microorganisms for the production of methacrylic acid
EP2373781A4 (en) 2008-12-16 2012-10-10 Genomatica Inc Microorganisms and methods for conversion of syngas and other carbon sources to useful products
MY176050A (en) 2009-04-30 2020-07-22 Genomatica Inc Organisms for the production of 1,3-butanediol
BR112012000659A2 (en) * 2009-06-26 2019-09-24 Gevo Inc recovery of higher alcohols from dilute aqueous solutions
US8968523B2 (en) * 2009-07-15 2015-03-03 Butamax Advanced Biofuels Llc Recovery of butanol isomers from a mixture of butanol isomers, water, and an organic extractant
US8968522B2 (en) 2009-07-15 2015-03-03 Butamax Advanced Biofuels Llc Recovery of butanol isomers from a mixture of butanol isomers, water, and an organic extractant
US8373008B2 (en) 2009-11-23 2013-02-12 Butamax (Tm) Advanced Biofuels Llc Recovery of butanol from a mixture of butanol, water, and an organic extractant
US8373009B2 (en) 2009-11-23 2013-02-12 Butamax (Tm) Advanced Biofuels Llc Recovery of butanol from a mixture of butanol, water, and an organic extractant
KR20120120493A (en) * 2009-12-10 2012-11-01 게노마티카 인코포레이티드 Methods and organisms for converting synthesis gas or other gaseous carbon sources and methanol to 1,3-butanediol
JP2013517796A (en) 2010-01-29 2013-05-20 ジェノマティカ・インコーポレイテッド Method and microorganism for biosynthesis of p-toluic acid and terephthalic acid
US8048661B2 (en) 2010-02-23 2011-11-01 Genomatica, Inc. Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes
US8445244B2 (en) 2010-02-23 2013-05-21 Genomatica, Inc. Methods for increasing product yields
US9023636B2 (en) 2010-04-30 2015-05-05 Genomatica, Inc. Microorganisms and methods for the biosynthesis of propylene
CA2797409C (en) 2010-05-05 2019-12-24 Genomatica, Inc. Microorganisms and methods for the biosynthesis of butadiene
US9605269B2 (en) 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
PH12013500158A1 (en) 2010-07-26 2013-03-11 Genomatica Inc Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
CA2838519A1 (en) * 2011-06-17 2012-12-20 Butamax Advanced Biofuels Llc Lignocellulosic hydrolysates as feedstocks for isobutanol fermentation
US20130109079A1 (en) * 2011-11-02 2013-05-02 Shell Oil Company Method for separating an organic component from a mixture containing the organic component
EP2844730A1 (en) 2012-05-04 2015-03-11 Butamax Advanced Biofuels LLC Processes and systems for alcohol production and recovery
US9024090B2 (en) 2012-12-19 2015-05-05 Celanese International Corporation Catalysts and processes for producing butanol
US8962897B2 (en) 2012-12-19 2015-02-24 Celanese International Corporation Catalysts and processes for producing butanol
US9765182B2 (en) * 2013-09-20 2017-09-19 The United States Of America, As Represented By The Secretary Of Agriculture Elastomer derived from epoxidized vegetable oil
US20150087041A1 (en) * 2013-09-26 2015-03-26 E I Du Pont De Nemours And Company Production of ethanol with reduced contaminants in a cellulosic biomass based process
US9018426B1 (en) 2013-12-19 2015-04-28 Celanese International Corporation Processes for producing multi-carbon alcohols
BR112021002573A2 (en) 2018-08-10 2021-05-11 Metabolic Explorer microorganisms with production of 1,3-propanediol and improved butyric acid

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2007041269A2 (en) * 2005-09-29 2007-04-12 E. I. Du Pont De Nemours And Company Fermentive production of four carbon alcohols

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
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BOYNTON ZHUANG L ET AL: "Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824", JOURNAL OF BACTERIOLOGY,, vol. 178, no. 11, 1 January 1996 (1996-01-01), pages 3015 - 3024, XP002486291 *
DREWKE C ET AL: "Ethanol formation in adh0 mutants reveals the existence of a novel acetaldehyde-reducing activity in Saccharomyces cerevisiae", JOURNAL OF BACTERIOLOGY JUL 1990,, vol. 172, no. 7, 1 July 1990 (1990-07-01), pages 3909 - 3917, XP002486946 *
EZEJI T C ET AL: "Acetone butanol ethanol (ABE) production from concentrated substrate: Reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 63, no. 6, February 2004 (2004-02-01), pages 653 - 658, XP002512560, ISSN: 0175-7598 *
LIU F ET AL: "Separation of acetone-butanol-ethanol (ABE) from dilute aqueous solutions by pervaporation", SEPARATION AND PURIFICATION TECHNOLOGY, ELSEVIER SCIENCE, AMSTERDAM, NL, vol. 42, no. 3, 1 April 2005 (2005-04-01), pages 273 - 282, XP004773276, ISSN: 1383-5866 *
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Also Published As

Publication number Publication date
BRPI0814640A2 (en) 2014-10-14
US20100205857A1 (en) 2010-08-19
CN101970673A (en) 2011-02-09
EP2171075A2 (en) 2010-04-07
WO2009013160A2 (en) 2009-01-29

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