WO2009017233A1 - 形質転換用酵母、形質転換方法及び物質生産方法 - Google Patents

形質転換用酵母、形質転換方法及び物質生産方法 Download PDF

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Publication number
WO2009017233A1
WO2009017233A1 PCT/JP2008/063900 JP2008063900W WO2009017233A1 WO 2009017233 A1 WO2009017233 A1 WO 2009017233A1 JP 2008063900 W JP2008063900 W JP 2008063900W WO 2009017233 A1 WO2009017233 A1 WO 2009017233A1
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WIPO (PCT)
Prior art keywords
transformation
yeast
transferred
target gene
producing substance
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Ceased
Application number
PCT/JP2008/063900
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English (en)
French (fr)
Inventor
Satoshi Saito
Kazushi Takahashi
Kayo Miyata
Akihiko Kondo
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Toyota Motor Corp
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Toyota Motor Corp
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Application filed by Toyota Motor Corp filed Critical Toyota Motor Corp
Priority to EP08792113A priority Critical patent/EP2186891A4/en
Priority to US12/671,459 priority patent/US20100190223A1/en
Publication of WO2009017233A1 publication Critical patent/WO2009017233A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　より多くのコピー数で目的遺伝子を導入することができる、或いはより多種類の目的遺伝子を導入することができる形質転換用酵母を提供する。 　ホモタリック性を有してなり、複数の選択マーカーを付与した形質転換用酵母に対して目的遺伝子を導入する工程と、上記形質転換用酵母における選択マーカーに従って、目的遺伝子が導入された株を選択する工程とを含み、上記形質転換用酵母におけるホモタリック性により上記目的遺伝子を多重に導入する。
PCT/JP2008/063900 2007-08-01 2008-08-01 形質転換用酵母、形質転換方法及び物質生産方法 Ceased WO2009017233A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08792113A EP2186891A4 (en) 2007-08-01 2008-08-01 YEAST FOR TRANSFORMATION, PROCESS FOR TRANSFORMATION AND PROCESS FOR PRODUCTION OF SUBSTANCE
US12/671,459 US20100190223A1 (en) 2007-08-01 2008-08-01 Yeast for transformation, transformation method, and method for producing substance

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-200978 2007-08-01
JP2007200978A JP2009034036A (ja) 2007-08-01 2007-08-01 形質転換用酵母、形質転換方法及び物質生産方法

Publications (1)

Publication Number Publication Date
WO2009017233A1 true WO2009017233A1 (ja) 2009-02-05

Family

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PCT/JP2008/063900 Ceased WO2009017233A1 (ja) 2007-08-01 2008-08-01 形質転換用酵母、形質転換方法及び物質生産方法

Country Status (4)

Country Link
US (1) US20100190223A1 (ja)
EP (1) EP2186891A4 (ja)
JP (1) JP2009034036A (ja)
WO (1) WO2009017233A1 (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009139349A1 (ja) * 2008-05-14 2009-11-19 Ｂｉｏ－ｅｎｅｒｇｙ株式会社 酵母細胞に遺伝子を導入する方法およびそのためのベクター
JP5845210B2 (ja) 2013-06-13 2016-01-20 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
IL241462A0 (en) 2015-09-10 2015-11-30 Yeda Res & Dev Heterologous engineering of betalain pigments in plants
JP6447583B2 (ja) 2016-06-24 2019-01-09 トヨタ自動車株式会社 組換え酵母、及びそれを用いたエタノールの製造方法
CA3111386A1 (en) 2018-09-06 2020-03-12 Yeda Research And Development Co. Ltd. Cellulose-synthase-like enzymes and uses thereof
JP7078900B2 (ja) 2018-10-05 2022-06-01 トヨタ自動車株式会社 形質転換酵母及びこれを用いたエタノールの製造方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07114689B2 (ja) 1989-12-22 1995-12-13 国税庁長官 栄養要求性実用醸造酵母
JP2003164295A (ja) * 2001-09-20 2003-06-10 Toyota Motor Corp 遺伝子高発現系
JP2003164294A (ja) * 2001-09-20 2003-06-10 Toyota Motor Corp Ｐｄｃ１プロモーター

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1437405B1 (en) * 2001-09-20 2009-12-23 Toyota Jidosha Kabushiki Kaisha Gene overexpression system

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07114689B2 (ja) 1989-12-22 1995-12-13 国税庁長官 栄養要求性実用醸造酵母
JP2003164295A (ja) * 2001-09-20 2003-06-10 Toyota Motor Corp 遺伝子高発現系
JP2003164294A (ja) * 2001-09-20 2003-06-10 Toyota Motor Corp Ｐｄｃ１プロモーター

Non-Patent Citations (22)

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"Methods in yeast genetics", 2000, A COLD SPRING HARBOR LABORATORY
"Screening for yeast having strong alcohol fermentation ability", JOURNAL OF THE BREWING SOCIETY OF JAPAN, vol. 82, no. 6, 1987, pages 439 - 443
"Various properties of alcohol yeast", SHU-KEN KAIHO, 1998, pages 18 - 22
AKADA ET AL., YEAST, vol. 23, 2006, pages 399 - 405
AKADA R. ET AL.: "PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae", YEAST, vol. 23, 2006, pages 399 - 405, XP009138985 *
AKADA R. ET AL.: "Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast", YEAST, vol. 19, 2002, pages 393 - 402, XP009138986 *
ALANI E. ET AL.: "A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains", GENETICS, vol. 116, 1987, pages 541 - 545, XP002108451 *
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 2005 (2005-05-01), pages 2789 - 2792
J. BACTERIOLOGY, vol. 153, 1983, pages 163
JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 81, no. 2, 1996, pages 98 - 103
KITAMOTO K. ET AL.: "Construction of uracil and tryptophan auxotrophic mutants from sake yeasts by disruption of URA3 and TRP1 genes", AGRIC. BIOL. CHEM., vol. 54, 1990, pages 2979 - 2987, XP009123603 *
METH. ENZYM., vol. 194, 1990, pages 182
MURAI, T ET AL.: "Assimilation of cellooligosaccharides by a cell surface-engineered yeast expressing [-glucosidase and carboxymethylcellulase from Aspergillus aculeatus", APPL. ENVIRON. MICROBIOL., vol. 64, 1998, pages 4857 - 4861
PROC. NATL. ACAD. SCI. U.S.A., vol. 75, 1978, pages 1929
SAITO ET AL., JOURNAL OF FERMENT. BIOENG., vol. 81, 1996, pages 98 - 103
SAITO S. ET AL.: "Breeding of a new type of baker's yeast by delta-integration for overproduction of glucoamylase using a homothallic yeast", J. FERMENT. BIOENG., vol. 81, 1996, pages 98 - 103, XP008131056 *
SAKAI A. ET AL.: "Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty", APPL. MICROBIOL. BIOTECHNOL., vol. 33, 1990, pages 302 - 306, XP009138993 *
See also references of EP2186891A4 *
YASUYA FUJITA ET AL.: "Direct and Efficient Production of Ethanol from Cellulosic Material with a Yeast Strain Displaying Cellulolytic Enzymes", APPL ENVIRON MICROBIOL., vol. 68, no. 10, October 2002 (2002-10-01), pages 5136 - 5141
YEAST, vol. 23, no. 5, pages 399 - 405
YIP C.W. ET AL.: "Development of a vector system for the cloning of the glucoamylase gene of Endomyces fibuliger", MICROBIAL UTILIZATION OF RENEWABLE RESOURCES, vol. 9, 1996, pages 334 - 341, XP009138990 *

Also Published As

Publication number Publication date
EP2186891A4 (en) 2010-11-03
US20100190223A1 (en) 2010-07-29
JP2009034036A (ja) 2009-02-19
EP2186891A1 (en) 2010-05-19

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