WO2009025209A1 - 組換えｄｎａの調製方法 - Google Patents

組換えｄｎａの調製方法 Download PDF

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Publication number
WO2009025209A1
WO2009025209A1 PCT/JP2008/064482 JP2008064482W WO2009025209A1 WO 2009025209 A1 WO2009025209 A1 WO 2009025209A1 JP 2008064482 W JP2008064482 W JP 2008064482W WO 2009025209 A1 WO2009025209 A1 WO 2009025209A1
Authority
WO
WIPO (PCT)
Prior art keywords
dna
interest
site
reaction
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/064482
Other languages
English (en)
French (fr)
Inventor
Takeharu Nagai
Ippei Kotera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hokkaido University NUC
Original Assignee
Hokkaido University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hokkaido University NUC filed Critical Hokkaido University NUC
Priority to US12/674,160 priority Critical patent/US20110244521A1/en
Priority to EP08792415A priority patent/EP2202313A4/en
Priority to JP2009529010A priority patent/JPWO2009025209A1/ja
Publication of WO2009025209A1 publication Critical patent/WO2009025209A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　【課題】 本発明の課題は、簡略化され、かつ効率が改善されたＤＮＡ組換え方法を提供することにある。 【解決手段】 上記課題は、ベクターＤＮＡに目的ＤＮＡ断片を挿入する組換えＤＮＡの調製方法であって、（a）ＤＮＡ認識部位とＤＮＡ切断部位とが異なる制限酵素の存在下、該ベクターＤＮＡの目的ＤＮＡ断片挿入部位と該目的ＤＮＡ断片を含むＤＮＡとを同時に切断する反応と、（b）ＤＮＡ連結酵素の存在下、該目的ＤＮＡ断片を該ベクターＤＮＡに挿入する反応を、同一の反応場所において実質的に同時に行う工程を含む、組換えＤＮＡの調製方法によって解決される。
PCT/JP2008/064482 2007-08-21 2008-08-12 組換えｄｎａの調製方法 Ceased WO2009025209A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/674,160 US20110244521A1 (en) 2007-08-21 2008-08-12 Method for preparation of recobinant dna
EP08792415A EP2202313A4 (en) 2007-08-21 2008-08-12 METHOD FOR PRODUCING RECOMBINANT DNA
JP2009529010A JPWO2009025209A1 (ja) 2007-08-21 2008-08-12 組換えｄｎａの調製方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-215238 2007-08-21
JP2007215238 2007-08-21

Publications (1)

Publication Number Publication Date
WO2009025209A1 true WO2009025209A1 (ja) 2009-02-26

Family

ID=40378115

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/064482 Ceased WO2009025209A1 (ja) 2007-08-21 2008-08-12 組換えｄｎａの調製方法

Country Status (4)

Country Link
US (1) US20110244521A1 (ja)
EP (1) EP2202313A4 (ja)
JP (1) JPWO2009025209A1 (ja)
WO (1) WO2009025209A1 (ja)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
US10253321B2 (en) 2013-05-01 2019-04-09 Dna2.0, Inc. Methods, compositions and kits for a one-step DNA cloning system
US9206433B2 (en) * 2013-05-01 2015-12-08 Dna Twopointo, Inc. Methods, compositions and kits for a one-step DNA cloning system
CN106795522A (zh) * 2014-10-17 2017-05-31 上海斯丹赛生物技术有限公司 定向克隆的方法
WO2016135300A1 (en) * 2015-02-26 2016-09-01 Qiagen Gmbh Efficiency improving methods for gene library generation

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998038326A1 (en) * 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
WO2001059099A1 (en) * 2000-02-14 2001-08-16 Hosch Stefan B A method of preparing recombinant nucleic acids
WO2005014796A2 (en) * 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
JP2007508012A (ja) 2003-10-03 2007-04-05 プロメガ　コーポレイション 方向性クローニング用ベクター
WO2008095927A1 (en) * 2007-02-05 2008-08-14 Philipps-Universität Marburg Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
WO1998038326A1 (en) * 1997-02-28 1998-09-03 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
WO2001059099A1 (en) * 2000-02-14 2001-08-16 Hosch Stefan B A method of preparing recombinant nucleic acids
WO2005014796A2 (en) * 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
JP2007508012A (ja) 2003-10-03 2007-04-05 プロメガ　コーポレイション 方向性クローニング用ベクター
WO2008095927A1 (en) * 2007-02-05 2008-08-14 Philipps-Universität Marburg Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"Molecular Cloning: A laboratory Manual", 2001, COLD SPRING HARBOR LABORATORY
FROMME T AND KLINGENSPOR M: "Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase", J. BIOL. ENG., vol. 1, no. 1, 26 November 2007 (2007-11-26), XP021040813 *
REBATCHOUK D ET AL: "NOMAD: A versatile strategy for in vitro DNA manipulation applied to promoter analysis and vector design", PROC. NATL. ACAD. SCI., vol. 93, 1996, USA, pages 10891 - 10896, XP002482732 *
See also references of EP2202313A4 *
SZYNBALSKI, W., GENE, vol. 40, 1985, pages 169

Also Published As

Publication number Publication date
EP2202313A4 (en) 2011-03-23
EP2202313A1 (en) 2010-06-30
US20110244521A1 (en) 2011-10-06
JPWO2009025209A1 (ja) 2010-11-25

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