WO2009028063A1 - Ｆｒｅｔ検出方法及び装置 - Google Patents

Ｆｒｅｔ検出方法及び装置 Download PDF

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Publication number
WO2009028063A1
WO2009028063A1 PCT/JP2007/066850 JP2007066850W WO2009028063A1 WO 2009028063 A1 WO2009028063 A1 WO 2009028063A1 JP 2007066850 W JP2007066850 W JP 2007066850W WO 2009028063 A1 WO2009028063 A1 WO 2009028063A1
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WO
WIPO (PCT)
Prior art keywords
molecule
accepter
fret
laser light
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2007/066850
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English (en)
French (fr)
Inventor
Noriaki Kimura
Shigeyuki Nakada
Kyouji Doi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsui Engineering and Shipbuilding Co Ltd
Original Assignee
Mitsui Engineering and Shipbuilding Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Engineering and Shipbuilding Co Ltd filed Critical Mitsui Engineering and Shipbuilding Co Ltd
Priority to CN2007800537170A priority Critical patent/CN101688837B/zh
Priority to EP07806327.8A priority patent/EP2196795A4/en
Priority to PCT/JP2007/066850 priority patent/WO2009028063A1/ja
Priority to KR1020097027237A priority patent/KR101152614B1/ko
Priority to US12/669,619 priority patent/US20100193703A1/en
Publication of WO2009028063A1 publication Critical patent/WO2009028063A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

　ドナー分子及びアクセプター分子からなるサンプルについて、短時間に多数のサンプルのＦＲＥＴ検出を行うとき、まず、周波数ｆ＋Δｆで強度変調したドナー分子励起用の第１のレーザ光をドナー分子に照射するとともに、周波数ｆで強度変調したアクセプター分子励起用の第２のレーザ光をアクセプター分子に照射し、アクセプター分子が発する蛍光を受光する。受光した蛍光の蛍光信号から、ＦＲＥＴによりアクセプター分子が発する蛍光の第１の信号成分と、第２のレーザ光の照射により励起したアクセプター分子が発する蛍光の第２の信号成分とを抽出する。抽出した第１の信号成分の位相遅れと、抽出した第２の信号成分の位相遅れとを算出し、これらの位相遅れに基づいて、ＦＲＥＴの発生の有無を判定する。
PCT/JP2007/066850 2007-08-30 2007-08-30 Ｆｒｅｔ検出方法及び装置 Ceased WO2009028063A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN2007800537170A CN101688837B (zh) 2007-08-30 2007-08-30 Fret检测方法及装置
EP07806327.8A EP2196795A4 (en) 2007-08-30 2007-08-30 FRET DETECTION METHOD AND DEVICE
PCT/JP2007/066850 WO2009028063A1 (ja) 2007-08-30 2007-08-30 Ｆｒｅｔ検出方法及び装置
KR1020097027237A KR101152614B1 (ko) 2007-08-30 2007-08-30 형광 공명 에너지 이동 검출 방법 및 장치
US12/669,619 US20100193703A1 (en) 2007-08-30 2007-08-30 Fret detection method and device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/066850 WO2009028063A1 (ja) 2007-08-30 2007-08-30 Ｆｒｅｔ検出方法及び装置

Publications (1)

Publication Number Publication Date
WO2009028063A1 true WO2009028063A1 (ja) 2009-03-05

Family

ID=40386807

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/066850 Ceased WO2009028063A1 (ja) 2007-08-30 2007-08-30 Ｆｒｅｔ検出方法及び装置

Country Status (5)

Country Link
US (1) US20100193703A1 (ja)
EP (1) EP2196795A4 (ja)
KR (1) KR101152614B1 (ja)
CN (1) CN101688837B (ja)
WO (1) WO2009028063A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200458306Y1 (ko) 2009-11-13 2012-03-13 한국생명공학연구원 고감도 휴대용 ｆｒｅｔ 형광측정 장치
CN102713577A (zh) * 2010-01-25 2012-10-03 三井造船株式会社 荧光检测装置以及荧光检测方法
US10393736B2 (en) 2016-04-01 2019-08-27 Emory University Anti-fouling saline and siloxane coated particles, substrates, polymers and uses related thereto

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102792147B (zh) * 2010-03-10 2015-05-13 贝克曼考尔特公司 在颗粒分析器中产生脉冲参数
US9501822B2 (en) 2011-01-28 2016-11-22 Cornell University Computer-implemented platform for automated fluorescence imaging and kinetic analysis
JP6166032B2 (ja) 2012-11-06 2017-07-19 浜松ホトニクス株式会社 半導体デバイス検査装置及び半導体デバイス検査方法
WO2016059635A1 (en) * 2014-10-13 2016-04-21 Glusense Ltd. Analyte-sensing device
EP3445872A1 (en) 2016-04-20 2019-02-27 Glusense Ltd. Fret-based glucose-detection molecules
US10679488B2 (en) 2017-04-05 2020-06-09 Microsensor Labs, LLC System and method for improving compliance with one or more protocols including hand hygiene and personal protective equipment protocols
CN115190969A (zh) * 2020-01-16 2022-10-14 耶路撒冷希伯来大学伊森姆研究发展有限公司 用于fret显微镜学中使用的系统和方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07229835A (ja) * 1994-02-16 1995-08-29 Hamamatsu Photonics Kk エネルギー移動検出法およびその装置
JPH10227739A (ja) * 1997-02-18 1998-08-25 Bunshi Bio Photonics Kenkyusho:Kk 被検体検出方法および装置
JPH10332594A (ja) * 1997-05-28 1998-12-18 Bunshi Baiohotonikusu Kenkyusho:Kk 被検体検出方法
JP2005207823A (ja) 2004-01-21 2005-08-04 Olympus Corp 蛍光分光分析の方法

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US5818582A (en) * 1996-09-19 1998-10-06 Ciencia, Inc. Apparatus and method for phase fluorometry
WO1998055026A1 (en) * 1997-06-05 1998-12-10 Kairos Scientific Inc. Calibration of fluorescence resonance energy transfer in microscopy
GB9718477D0 (en) * 1997-09-02 1997-11-05 Photonic Research Systems Limi Luminescence detection
GB9811483D0 (en) * 1998-05-29 1998-07-29 Photonic Research Systems Limi Luminescence assay using cyclical excitation wavelength sequence
CN1142423C (zh) * 2000-04-28 2004-03-17 华中理工大学 定量测量荧光共振能量转移效率的方法
US20030228703A1 (en) * 2002-04-05 2003-12-11 The Regents Of The University Of Michigan Fluorescence resonance energy transfer quantitation and stoichiometry in living cells
GB0416732D0 (en) * 2004-07-27 2004-09-01 Precisense As A method and apparatus for measuring the phase shift induced in a light signal by a sample

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07229835A (ja) * 1994-02-16 1995-08-29 Hamamatsu Photonics Kk エネルギー移動検出法およびその装置
JPH10227739A (ja) * 1997-02-18 1998-08-25 Bunshi Bio Photonics Kenkyusho:Kk 被検体検出方法および装置
JPH10332594A (ja) * 1997-05-28 1998-12-18 Bunshi Baiohotonikusu Kenkyusho:Kk 被検体検出方法
JP2005207823A (ja) 2004-01-21 2005-08-04 Olympus Corp 蛍光分光分析の方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2196795A4

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR200458306Y1 (ko) 2009-11-13 2012-03-13 한국생명공학연구원 고감도 휴대용 ｆｒｅｔ 형광측정 장치
CN102713577A (zh) * 2010-01-25 2012-10-03 三井造船株式会社 荧光检测装置以及荧光检测方法
US10393736B2 (en) 2016-04-01 2019-08-27 Emory University Anti-fouling saline and siloxane coated particles, substrates, polymers and uses related thereto

Also Published As

Publication number Publication date
US20100193703A1 (en) 2010-08-05
KR101152614B1 (ko) 2012-06-05
KR20100017945A (ko) 2010-02-16
CN101688837B (zh) 2011-09-28
EP2196795A1 (en) 2010-06-16
EP2196795A4 (en) 2014-04-02
CN101688837A (zh) 2010-03-31

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