WO2009028582A1 - イソプロパノール生産能を有する形質転換体 - Google Patents

イソプロパノール生産能を有する形質転換体 Download PDF

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Publication number
WO2009028582A1
WO2009028582A1 PCT/JP2008/065355 JP2008065355W WO2009028582A1 WO 2009028582 A1 WO2009028582 A1 WO 2009028582A1 JP 2008065355 W JP2008065355 W JP 2008065355W WO 2009028582 A1 WO2009028582 A1 WO 2009028582A1
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enzyme
gene encoding
foreign gene
activity
isopropanol
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Ceased
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English (en)
French (fr)
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Hideaki Yukawa
Masayuki Inui
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Honda Motor Co Ltd
Research Institute of Innovative Technology for the Earth RITE
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Honda Motor Co Ltd
Research Institute of Innovative Technology for the Earth RITE
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Priority to US12/733,366 priority Critical patent/US8426172B2/en
Priority to BRPI0816161-5A2A priority patent/BRPI0816161A2/pt
Priority to EP08828643A priority patent/EP2182051A4/en
Priority to JP2009530164A priority patent/JP5395667B2/ja
Publication of WO2009028582A1 publication Critical patent/WO2009028582A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

　以下の（ａ）～（ｄ）の遺伝子を好気性細菌または通性嫌気性細菌に導入したことを特徴とする、イソプロパノール生産能を有する形質転換体。 （ａ）アセチル-CoA アセチルトランスフェラーゼ（Acetyl-CoA acetyltransferase）活性を有する酵素をコードする外来性遺伝子 （ｂ）アセトアセチル-CoA：アセテート CoA-トランスフェラーゼ（Acetoacetyl CoA:acetate CoA transferase）活性を有する酵素をコードする外来性遺伝子 （ｃ）アセトアセテート デカルボキシラーゼ（Acetoacetate decarboxylase）活性を有する酵素をコードする外来性遺伝子 （ｄ）イソプロパノール デヒドロゲナーゼ（Isopropanol dehydrogenase）活性を有する酵素をコードする外来性遺伝子
PCT/JP2008/065355 2007-08-29 2008-08-28 イソプロパノール生産能を有する形質転換体 Ceased WO2009028582A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/733,366 US8426172B2 (en) 2007-08-29 2008-08-28 Transformant capable of producing isopropanol
BRPI0816161-5A2A BRPI0816161A2 (pt) 2007-08-29 2008-08-28 Transformante capaz de produzir isopropanol
EP08828643A EP2182051A4 (en) 2007-08-29 2008-08-28 FOR THE MANUFACTURE OF ISOPROPANOL SUITABLE TRANSFORMERS
JP2009530164A JP5395667B2 (ja) 2007-08-29 2008-08-28 イソプロパノール生産能を有する形質転換体

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007222633 2007-08-29
JP2007-222633 2007-08-29

Publications (1)

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WO2009028582A1 true WO2009028582A1 (ja) 2009-03-05

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PCT/JP2008/065355 Ceased WO2009028582A1 (ja) 2007-08-29 2008-08-28 イソプロパノール生産能を有する形質転換体

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US (1) US8426172B2 (ja)
EP (1) EP2182051A4 (ja)
JP (1) JP5395667B2 (ja)
BR (1) BRPI0816161A2 (ja)
WO (1) WO2009028582A1 (ja)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011031897A1 (en) * 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
WO2011111638A1 (ja) 2010-03-09 2011-09-15 三井化学株式会社 生産性の高いイソプロピルアルコール生産細菌
WO2011143592A1 (en) * 2010-05-13 2011-11-17 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of propanol
WO2011142027A1 (ja) 2010-05-14 2011-11-17 トヨタ自動車株式会社 イソプロパノールの製造方法及びイソプロパノール生産能を有する組換え酵母
JP2011239692A (ja) * 2010-05-14 2011-12-01 Toyota Motor Corp ２−ブタノールの製造方法及び２−ブタノール生産能を有する組換え微生物
WO2012020833A1 (ja) 2010-08-12 2012-02-16 三井化学株式会社 ｇｎｔＲを破壊することによって生産性が向上したイソプロピルアルコール生産細菌
JP2012524529A (ja) * 2009-04-23 2012-10-18 エボニック デグサ ゲーエムベーハー アセトン生成細胞およびアセトンを生成する方法
JP2012525156A (ja) * 2009-04-30 2012-10-22 ゲノマチカ， インク． イソプロパノール、ｎ−ブタノール、及びイソブタノールの産生のための微生物
JP2013544083A (ja) * 2010-10-29 2013-12-12 ノボザイムス アクティーゼルスカブ 組み換えｎ−プロパノール及びイソプロパノール生産
US9217183B2 (en) 2010-05-14 2015-12-22 Toyota Jidosha Kabushiki Kaisha Method for producing isopropanol and recombinant yeast capable of producing isopropanol
US9909150B2 (en) 2012-11-05 2018-03-06 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,2-propanediol, n-propanol, 1,3-propanediol, or glycerol related thereto

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2712779C (en) 2008-01-22 2021-03-16 Genomatica, Inc. Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
WO2012034023A2 (en) * 2010-09-10 2012-03-15 University Of Delaware Recombinant clostridia that fix co2 and co and uses thereof
FR2981089B1 (fr) * 2011-10-11 2016-05-20 Ifp Energies Now Production d'isopropanol par des souches recombinantes ameliorees
CN106554931B (zh) * 2015-09-30 2020-03-17 中国石油化工股份有限公司 一株拜氏羧菌及其应用

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JPS62166890A (ja) 1986-01-20 1987-07-23 Asahi Chem Ind Co Ltd イソクエン酸デヒドロゲナ−ゼ産生遺伝子を含むｄｎａ断片
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JP2012524529A (ja) * 2009-04-23 2012-10-18 エボニック デグサ ゲーエムベーハー アセトン生成細胞およびアセトンを生成する方法
JP2012525156A (ja) * 2009-04-30 2012-10-22 ゲノマチカ， インク． イソプロパノール、ｎ−ブタノール、及びイソブタノールの産生のための微生物
EP2933338A3 (en) * 2009-09-09 2016-01-06 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
WO2011031897A1 (en) * 2009-09-09 2011-03-17 Genomatica, Inc. Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
EP2475775A4 (en) * 2009-09-09 2013-03-20 Genomatica Inc MICROORGANISMS AND METHOD FOR THE SIMULTANEOUS PREPARATION OF ISOPROPANOL AND PRIMARY ALCOHOLS, DIOLES AND ACIDS
JP5628288B2 (ja) * 2010-03-09 2014-11-19 三井化学株式会社 生産性の高いイソプロピルアルコール生産細菌
WO2011111638A1 (ja) 2010-03-09 2011-09-15 三井化学株式会社 生産性の高いイソプロピルアルコール生産細菌
CN102791850B (zh) * 2010-03-09 2018-01-09 三井化学株式会社 生产能力高的异丙醇生产细菌
CN102791850A (zh) * 2010-03-09 2012-11-21 三井化学株式会社 生产能力高的异丙醇生产细菌
US8932845B2 (en) 2010-03-09 2015-01-13 Mitsui Chemicals, Inc. Highly productive isopropyl alcohol-producing bacterium
WO2011143592A1 (en) * 2010-05-13 2011-11-17 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of propanol
US9217183B2 (en) 2010-05-14 2015-12-22 Toyota Jidosha Kabushiki Kaisha Method for producing isopropanol and recombinant yeast capable of producing isopropanol
US8828693B2 (en) 2010-05-14 2014-09-09 Toyora Jidosha Kabushiki Kaisha Method for producing isopropanol and recombinant yeast capable of producing isopropanol
JP5630501B2 (ja) * 2010-05-14 2014-11-26 トヨタ自動車株式会社 イソプロパノールの製造方法及びイソプロパノール生産能を有する組換え酵母
JP2011239692A (ja) * 2010-05-14 2011-12-01 Toyota Motor Corp ２−ブタノールの製造方法及び２−ブタノール生産能を有する組換え微生物
WO2011142027A1 (ja) 2010-05-14 2011-11-17 トヨタ自動車株式会社 イソプロパノールの製造方法及びイソプロパノール生産能を有する組換え酵母
CN103068968B (zh) * 2010-08-12 2014-07-23 三井化学株式会社 通过破坏gntR而提高了生产能力的异丙醇生产细菌
CN103068968A (zh) * 2010-08-12 2013-04-24 三井化学株式会社 通过破坏gntR而提高了生产能力的异丙醇生产细菌
JP5674789B2 (ja) * 2010-08-12 2015-02-25 三井化学株式会社 ｇｎｔＲを破壊することによって生産性が向上したイソプロピルアルコール生産細菌
WO2012020833A1 (ja) 2010-08-12 2012-02-16 三井化学株式会社 ｇｎｔＲを破壊することによって生産性が向上したイソプロピルアルコール生産細菌
US9267156B2 (en) 2010-08-12 2016-02-23 Mitsui Chemicals, Inc. Isopropyl alcohol-producing bacterium having improved productivity by GntR destruction
JP2013544083A (ja) * 2010-10-29 2013-12-12 ノボザイムス アクティーゼルスカブ 組み換えｎ−プロパノール及びイソプロパノール生産
US9909150B2 (en) 2012-11-05 2018-03-06 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,2-propanediol, n-propanol, 1,3-propanediol, or glycerol related thereto
US11629363B2 (en) 2012-11-05 2023-04-18 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,2-propanediol, n-propanol, 1,3-propanediol, or glycerol related thereto

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US20100203604A1 (en) 2010-08-12
BRPI0816161A2 (pt) 2014-10-14
EP2182051A1 (en) 2010-05-05
US8426172B2 (en) 2013-04-23
EP2182051A4 (en) 2010-12-29
JP5395667B2 (ja) 2014-01-22
JPWO2009028582A1 (ja) 2010-12-02

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