WO2009041415A1 - 新規グルコース脱水素酵素 - Google Patents

新規グルコース脱水素酵素 Download PDF

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Publication number
WO2009041415A1
WO2009041415A1 PCT/JP2008/067154 JP2008067154W WO2009041415A1 WO 2009041415 A1 WO2009041415 A1 WO 2009041415A1 JP 2008067154 W JP2008067154 W JP 2008067154W WO 2009041415 A1 WO2009041415 A1 WO 2009041415A1
Authority
WO
WIPO (PCT)
Prior art keywords
nadp
coenzyme
glucose dehydrogenase
gene
nad
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/067154
Other languages
English (en)
French (fr)
Inventor
Shigeru Kawano
Yoshihiko Yasohara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaneka Corp
Original Assignee
Kaneka Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp filed Critical Kaneka Corp
Priority to EP08833102.0A priority Critical patent/EP2202303B1/en
Priority to JP2009534326A priority patent/JP5603076B2/ja
Priority to US12/680,050 priority patent/US9303280B2/en
Priority to DK08833102.0T priority patent/DK2202303T3/en
Publication of WO2009041415A1 publication Critical patent/WO2009041415A1/ja
Anticipated expiration legal-status Critical
Priority to US15/051,813 priority patent/US9587228B2/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Emergency Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　本発明は、補酵素としてＮＡＤＰを用いた際の酵素活性値を、補酵素としてＮＡＤを用いた際の酵素活性値で割った値（ＮＡＤＰ／ＮＡＤ活性比）が３００以上であるグルコース脱水素酵素、その遺伝子、当該遺伝子を導入された形質転換体に関する。本発明の酵素はＮＡＤＰに対する特異性が極めて高く、ＮＡＤＰＨの生産、酵素的還元反応における補酵素再生、および、グルコース濃度を測定するためのバイオセンサー等に好適に利用し得る。
PCT/JP2008/067154 2007-09-26 2008-09-24 新規グルコース脱水素酵素 Ceased WO2009041415A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP08833102.0A EP2202303B1 (en) 2007-09-26 2008-09-24 Novel glucose dehydrogenase
JP2009534326A JP5603076B2 (ja) 2007-09-26 2008-09-24 新規グルコース脱水素酵素
US12/680,050 US9303280B2 (en) 2007-09-26 2008-09-24 DNA coding for a novel glucose dehydrogenase
DK08833102.0T DK2202303T3 (en) 2007-09-26 2008-09-24 NEW GLUCOSEDE HYDROGENASE
US15/051,813 US9587228B2 (en) 2007-09-26 2016-02-24 Vector containing a DNA coding for a novel glucose dehydrogenase and method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-249118 2007-09-26
JP2007249118 2007-09-26
JP2008037811 2008-02-19
JP2008-037811 2008-02-19

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/680,050 A-371-Of-International US9303280B2 (en) 2007-09-26 2008-09-24 DNA coding for a novel glucose dehydrogenase
US15/051,813 Division US9587228B2 (en) 2007-09-26 2016-02-24 Vector containing a DNA coding for a novel glucose dehydrogenase and method

Publications (1)

Publication Number Publication Date
WO2009041415A1 true WO2009041415A1 (ja) 2009-04-02

Family

ID=40511299

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/067154 Ceased WO2009041415A1 (ja) 2007-09-26 2008-09-24 新規グルコース脱水素酵素

Country Status (5)

Country Link
US (2) US9303280B2 (ja)
EP (1) EP2202303B1 (ja)
JP (1) JP5603076B2 (ja)
DK (1) DK2202303T3 (ja)
WO (1) WO2009041415A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012063843A1 (ja) 2010-11-09 2012-05-18 株式会社カネカ ハロゲン化インデノン類及びそれを用いた光学活性インダノン類又は光学活性インダノール類の製造方法
WO2013002277A1 (ja) 2011-06-28 2013-01-03 株式会社カネカ 酵素機能改変方法及びその変異体

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015060150A1 (ja) * 2013-10-21 2015-04-30 東洋紡株式会社 新規なグルコースデヒドロゲナーゼ
US10913971B2 (en) 2015-04-09 2021-02-09 Toyobo Co., Ltd. Enzyme preparation for use in measurement of glucose

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63109773A (ja) 1986-05-02 1988-05-14 Toyo Jozo Co Ltd グルコースデヒドロゲナーゼの製造方法
WO1994003613A1 (fr) 1992-08-10 1994-02-17 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Adn codant pour la decarbamylase a thermostabilite accrue et utilisation de cet adn
WO1998035025A1 (en) 1997-02-07 1998-08-13 Kaneka Corporation Novel carbonyl reductase, gene that encodes the same, and method of utilizing these
WO2002010399A1 (en) * 2000-08-01 2002-02-07 Kaneka Corporation Novel carbonyl reductase, gene thereof and method of using the same
WO2005123921A1 (ja) 2004-06-21 2005-12-29 Kaneka Corporation 新規グリセロール脱水素酵素、その遺伝子、及びその利用法
WO2006046455A1 (ja) * 2004-10-27 2006-05-04 Kaneka Corporation 新規カルボニル還元酵素、その遺伝子、およびその利用法
WO2006090814A1 (ja) 2005-02-25 2006-08-31 Kaneka Corporation 光学活性２級アルコールの製造方法
JP2006262767A (ja) 2005-03-24 2006-10-05 Kaneka Corp 新規グルコース脱水素酵素

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1235069B1 (en) * 1999-11-16 2006-06-28 Matsushita Electric Industrial Co., Ltd. Biosensor

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63109773A (ja) 1986-05-02 1988-05-14 Toyo Jozo Co Ltd グルコースデヒドロゲナーゼの製造方法
WO1994003613A1 (fr) 1992-08-10 1994-02-17 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Adn codant pour la decarbamylase a thermostabilite accrue et utilisation de cet adn
WO1998035025A1 (en) 1997-02-07 1998-08-13 Kaneka Corporation Novel carbonyl reductase, gene that encodes the same, and method of utilizing these
WO2002010399A1 (en) * 2000-08-01 2002-02-07 Kaneka Corporation Novel carbonyl reductase, gene thereof and method of using the same
WO2005123921A1 (ja) 2004-06-21 2005-12-29 Kaneka Corporation 新規グリセロール脱水素酵素、その遺伝子、及びその利用法
WO2006046455A1 (ja) * 2004-10-27 2006-05-04 Kaneka Corporation 新規カルボニル還元酵素、その遺伝子、およびその利用法
WO2006090814A1 (ja) 2005-02-25 2006-08-31 Kaneka Corporation 光学活性２級アルコールの製造方法
JP2006262767A (ja) 2005-03-24 2006-10-05 Kaneka Corp 新規グルコース脱水素酵素

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
"Basic Courses in Microbiology 8: Genetic Engineering", KYORITSU SHUPPAN, article "Biseibutsugaku Kiso Koza 8: Idensikogaku"
"Current Protocols in Molecular Biology", 1989, JOHN WILEY AND SONS, INC.
"Current Protocols in Molecular Biology", GREENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
"Koso Handobukku", 1982, ASAKURA
"Molecular Cloning", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Molecular Cloning, A laboratory manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Program Manual for The Wisconsin Package", September 1994, GENETICS COMPUTER GROUP
ARCH. BIOCHEM. BIOPHYS., vol. 228, no. 1, 1984, pages 113 - 119
HOKKAIDO INDUSTRIAL RESEARCH INSTITUTE REPORT, vol. 293, 1994, pages 141 - 145
J. BACTERIOL., vol. 184, no. 3, 2002, pages 672 - 678
J. BACTERIOL., vol. 90, no. 3, 1965, pages 653 - 660
METHODS ENZYMOL., vol. 89, 1982, pages 159 - 163
NUCL. ACIDS RES., vol. 16, 1988, pages 8186
RICE, P.; PETER RICE: "Program Manual for EGCG Package", 1996, HINXTON HALL
VISSER ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 53, 2000, pages 415
YASOHARA Y.: "Bio Fusei Kangen System", KAGAKU TO SEIBUTSU, vol. 44, no. 9, 2006, pages 629 - 632 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012063843A1 (ja) 2010-11-09 2012-05-18 株式会社カネカ ハロゲン化インデノン類及びそれを用いた光学活性インダノン類又は光学活性インダノール類の製造方法
WO2013002277A1 (ja) 2011-06-28 2013-01-03 株式会社カネカ 酵素機能改変方法及びその変異体

Also Published As

Publication number Publication date
JPWO2009041415A1 (ja) 2011-01-27
EP2202303B1 (en) 2016-10-26
EP2202303A4 (en) 2010-11-10
US9303280B2 (en) 2016-04-05
US20130183716A1 (en) 2013-07-18
US9587228B2 (en) 2017-03-07
JP5603076B2 (ja) 2014-10-08
US20160168544A1 (en) 2016-06-16
DK2202303T3 (en) 2017-02-06
EP2202303A1 (en) 2010-06-30

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