WO2009072593A1 - 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法 - Google Patents

乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法 Download PDF

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Publication number
WO2009072593A1
WO2009072593A1 PCT/JP2008/072129 JP2008072129W WO2009072593A1 WO 2009072593 A1 WO2009072593 A1 WO 2009072593A1 JP 2008072129 W JP2008072129 W JP 2008072129W WO 2009072593 A1 WO2009072593 A1 WO 2009072593A1
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WIPO (PCT)
Prior art keywords
lactic acid
expression cassette
lactate dehydrogenase
yeast
producing lactic
Prior art date
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Ceased
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PCT/JP2008/072129
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English (en)
French (fr)
Inventor
Kenji Sawai
Hideki Sawai
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Toray Industries Inc
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Toray Industries Inc
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Publication date
Application filed by Toray Industries Inc filed Critical Toray Industries Inc
Priority to BRPI0818979-0A2A priority Critical patent/BRPI0818979A2/pt
Priority to CN200880126352.4A priority patent/CN101939426B/zh
Priority to EP08856191.5A priority patent/EP2221373B1/en
Priority to ES08856191.5T priority patent/ES2616428T3/es
Priority to JP2009504930A priority patent/JP5532919B2/ja
Priority to US12/746,642 priority patent/US8859260B2/en
Priority to AU2008332348A priority patent/AU2008332348B2/en
Priority to CA2707687A priority patent/CA2707687A1/en
Publication of WO2009072593A1 publication Critical patent/WO2009072593A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　高い光学純度、乳酸収率ならびに乳酸生産速度を同時に達成することが可能となる、乳酸生産能力を有する酵母を分離膜で濾過しながら行う連続培養において、乳酸収率ならびに乳酸生産速度の低下を防ぐために必要となる乳酸脱水素酵素をコードする遺伝子発現カセット、該カセットを有する酵母ならびに該酵母を培養することによる乳酸の製造方法を提供する。 　本発明の乳酸脱水素酵素発現カセットは、プロモーターの下流に乳酸脱水素酵素をコードする遺伝子が連結された乳酸脱水素酵素発現カセットであって、乳酸生産能力を有する酵母を分離膜で濾過しながら行う連続培養において、前記プロモーターは、培養開始後５０時間以降における遺伝子発現量が全遺伝子の平均相対発現量の５倍以上である遺伝子のプロモーターである、乳酸脱水素酵素発現カセットである。
PCT/JP2008/072129 2007-12-07 2008-12-05 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法 Ceased WO2009072593A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BRPI0818979-0A2A BRPI0818979A2 (pt) 2007-12-07 2008-12-05 "cassete de expressão de lactato desidrogenase, cepa de levedura transformante e método de produzir ácido láctico"
CN200880126352.4A CN101939426B (zh) 2007-12-07 2008-12-05 乳酸脱氢酶表达盒、转化酵母和乳酸的制造方法
EP08856191.5A EP2221373B1 (en) 2007-12-07 2008-12-05 Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
ES08856191.5T ES2616428T3 (es) 2007-12-07 2008-12-05 Casete de expresión para lactasa deshidrogenasa, levadura transformada y método para producir ácido láctico
JP2009504930A JP5532919B2 (ja) 2007-12-07 2008-12-05 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法
US12/746,642 US8859260B2 (en) 2007-12-07 2008-12-05 Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
AU2008332348A AU2008332348B2 (en) 2007-12-07 2008-12-05 Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
CA2707687A CA2707687A1 (en) 2007-12-07 2008-12-05 Lactate dehydrogenase-expressing cassette, transformant yeast strain and method of producing lactic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-317566 2007-12-07
JP2007317566 2007-12-07

Publications (1)

Publication Number Publication Date
WO2009072593A1 true WO2009072593A1 (ja) 2009-06-11

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Family Applications (1)

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PCT/JP2008/072129 Ceased WO2009072593A1 (ja) 2007-12-07 2008-12-05 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法

Country Status (10)

Country Link
US (1) US8859260B2 (ja)
EP (1) EP2221373B1 (ja)
JP (1) JP5532919B2 (ja)
KR (1) KR20100094525A (ja)
CN (1) CN101939426B (ja)
AU (1) AU2008332348B2 (ja)
BR (1) BRPI0818979A2 (ja)
CA (1) CA2707687A1 (ja)
ES (1) ES2616428T3 (ja)
WO (1) WO2009072593A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8822195B2 (en) 2009-06-03 2014-09-02 Toray Industries, Inc. Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid
JP2017023953A (ja) * 2015-07-23 2017-02-02 株式会社東芝 処理装置及び処理方法
CN113699090A (zh) * 2021-09-09 2021-11-26 浙江华睿生物技术有限公司 一种构建丙酸生产菌的方法

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KR101483470B1 (ko) * 2008-02-04 2015-01-16 도레이 카부시키가이샤 연속 발효에 의한 유산의 제조방법
KR20130001103A (ko) * 2011-06-24 2013-01-03 삼성전자주식회사 젖산의 고효율 생산을 위한 변형 미생물
US9150835B2 (en) 2011-06-24 2015-10-06 Samsung Electronics Co., Ltd. Modified microorganism for highly efficient production of lactic acid
FR2988733B1 (fr) * 2012-03-27 2016-02-05 Carbios Microorganisme recombinant
US9593350B2 (en) 2014-02-14 2017-03-14 Samsung Electronics Co., Ltd. Lactate dehydrogenase mutant, polynucleotide coding for the mutant, yeast cell including the polynucleotide, method of preparing the mutant, and method of producing the lactate using the same
LT3143075T (lt) 2014-05-16 2022-09-12 Carbios Mišrių pet plastiko gaminių perdirbimo būdas
PL3209771T3 (pl) 2014-10-21 2021-05-31 Carbios Polipeptyd wykazujący działanie rozkładające poliester i jego zastosowania
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
EP3268469A1 (en) 2015-03-13 2018-01-17 Carbios New polypeptide having a polyester degrading activity and uses thereof
CN107709457B (zh) 2015-06-12 2021-05-25 卡比奥斯公司 可生物降解聚酯组合物及其用途
US10717996B2 (en) 2015-12-21 2020-07-21 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
CN116199935A (zh) 2016-05-19 2023-06-02 卡比奥斯公司 一种降解塑料产品的方法
CN109943492B (zh) * 2019-04-03 2022-08-16 广东省微生物研究所（广东省微生物分析检测中心） 一种重组酵母菌株及其应用

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JP2007020538A (ja) * 2005-07-21 2007-02-01 Gekkeikan Sake Co Ltd タンパク質の高発現システム
JP2007082490A (ja) * 2005-09-22 2007-04-05 Toyota Central Res & Dev Lab Inc 有機酸の抽出発酵による生産方法及び該生産方法に用いる有機酸生産酵母
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8822195B2 (en) 2009-06-03 2014-09-02 Toray Industries, Inc. Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid
JP2017023953A (ja) * 2015-07-23 2017-02-02 株式会社東芝 処理装置及び処理方法
CN113699090A (zh) * 2021-09-09 2021-11-26 浙江华睿生物技术有限公司 一种构建丙酸生产菌的方法
CN113699090B (zh) * 2021-09-09 2023-06-20 浙江华睿生物技术有限公司 一种构建丙酸生产菌的方法

Also Published As

Publication number Publication date
EP2221373B1 (en) 2017-02-15
CA2707687A1 (en) 2009-06-11
BRPI0818979A2 (pt) 2014-10-14
EP2221373A1 (en) 2010-08-25
CN101939426A (zh) 2011-01-05
AU2008332348B2 (en) 2014-06-26
US8859260B2 (en) 2014-10-14
US20100273225A1 (en) 2010-10-28
JP5532919B2 (ja) 2014-06-25
JPWO2009072593A1 (ja) 2011-04-28
AU2008332348A1 (en) 2009-06-11
KR20100094525A (ko) 2010-08-26
ES2616428T3 (es) 2017-06-13
EP2221373A4 (en) 2011-07-06
CN101939426B (zh) 2014-01-08

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