WO2009078018A3 - Methods for purifying or depleting molecules or cells of interest - Google Patents

Methods for purifying or depleting molecules or cells of interest Download PDF

Info

Publication number
WO2009078018A3
WO2009078018A3 PCT/IL2008/001631 IL2008001631W WO2009078018A3 WO 2009078018 A3 WO2009078018 A3 WO 2009078018A3 IL 2008001631 W IL2008001631 W IL 2008001631W WO 2009078018 A3 WO2009078018 A3 WO 2009078018A3
Authority
WO
WIPO (PCT)
Prior art keywords
interest
cell
target molecule
volume
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2008/001631
Other languages
French (fr)
Other versions
WO2009078018A2 (en
Inventor
Jacob Falewich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affisink Biotechnology Ltd
Original Assignee
Affisink Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affisink Biotechnology Ltd filed Critical Affisink Biotechnology Ltd
Priority to US12/808,679 priority Critical patent/US20100311159A1/en
Priority to CA2708836A priority patent/CA2708836A1/en
Priority to EP08863008A priority patent/EP2244800A2/en
Publication of WO2009078018A2 publication Critical patent/WO2009078018A2/en
Publication of WO2009078018A3 publication Critical patent/WO2009078018A3/en
Priority to IL206372A priority patent/IL206372A0/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of separating a target molecule or cell of interest from a sample comprising contacting a sample including the target molecule or cell of interest with a non-immobilized coordinator ion or molecule, and a non-immobilized composition which comprises at least one ligand capable of binding directly or indirectly the target molecule or cell of interest, the at least one ligand being attached to at least two coordinating moieties and the target molecule or cell of interest, applying a gravitational or centrifugal force to concentrate at least 70% of the non-covalent complex in no more than 10 % of the volume as a suspension, resulting in a solute phase separation between the no more than 10 % of the volume and a remaining of the volume, collecting or disopsing the no more than 10 % of the volume, thereby separating the target molecule or cell of interest from the sample.
PCT/IL2008/001631 2007-12-17 2008-12-17 Methods for purifying or depleting molecules or cells of interest Ceased WO2009078018A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/808,679 US20100311159A1 (en) 2007-12-17 2008-12-17 Methods for purifying or depleting molecules or cells of interest
CA2708836A CA2708836A1 (en) 2007-12-17 2008-12-17 Methods for purifying or depleting molecules or cells of interest
EP08863008A EP2244800A2 (en) 2007-12-17 2008-12-17 Methods for purifying or depleting molecules or cells of interest
IL206372A IL206372A0 (en) 2007-12-17 2010-06-14 Methods for purifying or depleting molecules or cells of interest

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US606707P 2007-12-17 2007-12-17
US61/006,067 2007-12-17

Publications (2)

Publication Number Publication Date
WO2009078018A2 WO2009078018A2 (en) 2009-06-25
WO2009078018A3 true WO2009078018A3 (en) 2010-03-11

Family

ID=40795967

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/001631 Ceased WO2009078018A2 (en) 2007-12-17 2008-12-17 Methods for purifying or depleting molecules or cells of interest

Country Status (4)

Country Link
US (1) US20100311159A1 (en)
EP (1) EP2244800A2 (en)
CA (1) CA2708836A1 (en)
WO (1) WO2009078018A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024110444A1 (en) 2022-11-21 2024-05-30 Chreto Aps Method for purification of a target product using affinity purification technology

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840858A (en) * 1993-07-09 1998-11-24 T Cell Sciences, Inc. Protein purification using immobilized metal affinity chromatography for complement receptor proteins
US5935823A (en) * 1990-02-15 1999-08-10 The University Of North Carolina At Chapel Hill Totally synthetic affinity reagents
US20020166815A1 (en) * 2001-03-07 2002-11-14 Macfarlane Ronald D. Density gradient solutions of metal ion chelate complexes
US20060121519A1 (en) * 2003-07-24 2006-06-08 Affisink Biotechnology Ltd. Compositions and methods for purifying and crystallizing molecules of interest

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5935823A (en) * 1990-02-15 1999-08-10 The University Of North Carolina At Chapel Hill Totally synthetic affinity reagents
US5840858A (en) * 1993-07-09 1998-11-24 T Cell Sciences, Inc. Protein purification using immobilized metal affinity chromatography for complement receptor proteins
US20020166815A1 (en) * 2001-03-07 2002-11-14 Macfarlane Ronald D. Density gradient solutions of metal ion chelate complexes
US20060121519A1 (en) * 2003-07-24 2006-06-08 Affisink Biotechnology Ltd. Compositions and methods for purifying and crystallizing molecules of interest

Also Published As

Publication number Publication date
CA2708836A1 (en) 2009-06-25
WO2009078018A2 (en) 2009-06-25
EP2244800A2 (en) 2010-11-03
US20100311159A1 (en) 2010-12-09

Similar Documents

Publication Publication Date Title
WO2013124474A3 (en) Chromatographic isolation of cells and other complex biological materials
WO2009020609A3 (en) Isolation of nucleic acids molecules using modified solid supports
WO2004036189A3 (en) Methods for separating molecules
WO2009083804A3 (en) Improved fibronectin-based binding molecules and their use
WO2009125971A3 (en) Automatic refining apparatus, multi-well plate kit and method for extracting hexane from biological samples
WO2009105670A3 (en) Substrates for multiplexed assays and uses thereof
NZ571352A (en) Separation of nucleic acids from a liquid sample using a liquid flow device
WO2008156124A1 (en) Method for separation of immunoglobulin monomer
WO2008028081A3 (en) Sperm cell separation methods and compositions containing aptamers or nucleic acid sequences for use therein
WO2003035233A1 (en) Separating device, analysis system, separation method and method for manufacture of separating device
FR2925366B1 (en) AGGLOMERATED ZEOLITIC ADSORBENTS, PROCESS FOR THEIR PREPARATION AND USES THEREOF
WO2012145414A3 (en) Apparatus and method for separating and concentrating fluids containing multiple components
EP2412433A3 (en) Grafting method to improve chromatography media performance
WO2009094772A8 (en) Methods and apparatus for particle introduction and recovery
WO2007017085A3 (en) Process for cross-linking cellulose ester membranes
FR2925367B1 (en) AGGLOMERATED ZEOLITIC ADSORBENTS, PROCESS FOR THEIR PREPARATION AND USES THEREOF
WO2009024726A3 (en) Method for purifying or detecting a target protein
EP1903338A3 (en) Method and Apparatus for Isolating and Purifying Nucleic Acid by Single Bead
WO2009010976A3 (en) Compositions and methods for purifying and crystallizing molecules of interest
WO2008066858A3 (en) Method of separating target dna from mixed dna
WO2005073711A3 (en) Chromatographic material for the absorption of proteins at physiological ionic strength
WO2012099949A3 (en) Antibody purification via affinity chromatography
Link et al. Purifying protein complexes for mass spectrometry: applications to protein translation
WO2009005547A3 (en) Method for electrostatic trapping of molecules in nanopores
WO2009061799A3 (en) Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08863008

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2708836

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 12808679

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008863008

Country of ref document: EP