WO2009082050A1 - Procédé pour purifier de l'acide succinique par cristallisation d'un bouillon de culture - Google Patents

Procédé pour purifier de l'acide succinique par cristallisation d'un bouillon de culture Download PDF

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Publication number
WO2009082050A1
WO2009082050A1 PCT/KR2008/000238 KR2008000238W WO2009082050A1 WO 2009082050 A1 WO2009082050 A1 WO 2009082050A1 KR 2008000238 W KR2008000238 W KR 2008000238W WO 2009082050 A1 WO2009082050 A1 WO 2009082050A1
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WO
WIPO (PCT)
Prior art keywords
succinic acid
culture broth
recovering
succiniciproducens
concentration
Prior art date
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Ceased
Application number
PCT/KR2008/000238
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English (en)
Inventor
Won Hi Hong
Sang Yup Lee
Yeon Ki Hong
Hyo Jin Won
Yun Suk Huh
Hyohak Song
Eun Zoo Lee
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Korea Advanced Institute of Science and Technology KAIST
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Korea Advanced Institute of Science and Technology KAIST
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Application filed by Korea Advanced Institute of Science and Technology KAIST filed Critical Korea Advanced Institute of Science and Technology KAIST
Publication of WO2009082050A1 publication Critical patent/WO2009082050A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
    • C07C51/44—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation by distillation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
    • C07C51/42—Separation; Purification; Stabilisation; Use of additives
    • C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C55/00—Saturated compounds having more than one carboxyl group bound to acyclic carbon atoms
    • C07C55/02—Dicarboxylic acids
    • C07C55/10—Succinic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • Succinic acid which is a divalent organic acid consisting of 4 carbons, has been widely used in producing foods, medicines, cosmetics, solvents and the like, and has a high utility as a precursor of various, industrially important chemical products, and thus, the demand for succinic acid is expected to be dramatically increased (Zeikus et al, Appl. Microbiol. Biotechnol., 51 :545, 1999; Song et al, Enzyme Microbial Technol., 39: 352, 2006; Shekhawat et al, Biores. Technol., 97: 342, 2006; McKinlay et al., Appl. Microbiol. Biotechnol, 76:727, 2007).
  • the present invention relates to a method for recovering succinic acid from a culture broth of a succinic acid-producing microorganism, which comprises the steps of (a) concentrating the culture broth from which a succinic acid- producing microorganism is removed; (b) acidifying the concentrated culture broth; and (c) recovering the crystal of succinic acid by cooling down the acidified culture broth.
  • the separation and purification method according to the present invention enables an increase in the yield and purify of succinic acid up to 74.65% and 99.99% , respectively, thus making it possible to achieve technical effect unprecedented in the prior art, as well as, has advantages in cost reduction and environmental aspects. Therefore, the separation and purification method of the present invention is expected to highly contribute to replace current chemical processes for succinic acid production with bio-based succinic acid production processes.
  • the following examples illustrate only a succinic acid-producing microorganism Mannheimia sp., and fed-batch culture method, but, it is obvious to a person whom skilled in the art that other kinds of succinic acid-producing microorganisms and culture methods can also be used.
  • the crystallization process is preferably performed in the range of pH 1.5-2.0.
  • Table 2 The composition of organic acids present in initial culture broth, and a comparison of dry weight of organic acids including succinic acid between before and after the inventive separation and purification process, based on 1.0L of the culture broth obtained in Example 1.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention se rapporte à un procédé pour séparer et purifier de l'acide succinique avec un haut degré de pureté et un haut rendement par cristallisation d'un bouillon de culture et plus particulièrement un procédé pour recueillir de l'acide succinique avec un haut degré de pureté et un haut rendement qui comprend la concentration d'un bouillon de culture duquel on élimine un micro-organisme produisant de l'acide succinique puis l'addition d'une solution acide à basse température puis la cristallisation directe sans autre procédé de prétraitement. Selon la présente invention, on sépare l'acide succinique et on le purifie en utilisant un bouillon de culture à partir duquel on élimine un micro-organisme produisant de l'acide succinique, sans autre procédé de prétraitement, ce qui permet de faire des économies grâce au procédé simplifié et prévient toute pollution écologique en empêchant la génération de boue pendant le processus de récupération de l'acide succinique. De plus, l'acide succinique a été recueilli avec une grande pureté et un rendement élevé, ce qui a permis d'obtenir un effet technique sans précédent dans l'art antérieur en termes d'économie des coûts.
PCT/KR2008/000238 2007-12-20 2008-01-15 Procédé pour purifier de l'acide succinique par cristallisation d'un bouillon de culture Ceased WO2009082050A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070134710A KR20090066958A (ko) 2007-12-20 2007-12-20 배양액의 결정화에 의한 숙신산 정제방법
KR10-2007-0134710 2007-12-20

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WO2009082050A1 true WO2009082050A1 (fr) 2009-07-02

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KR (1) KR20090066958A (fr)
WO (1) WO2009082050A1 (fr)

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011064151A1 (fr) 2009-11-24 2011-06-03 Dsm Ip Assets B.V. Procédé de cristallisation d'acide succinique
EP2371802A1 (fr) 2010-03-30 2011-10-05 DSM IP Assets B.V. Procédé de cristallisation d'acide succinique
US8084626B1 (en) 2010-04-01 2011-12-27 Bioamber S.A.S. Processes for the production of hydrogenated products
US20120021473A1 (en) * 2010-04-01 2012-01-26 Bioamber S.A.S. Processes for producing carboxylic acids from fermentation broths containing their ammonium salts
US8246792B2 (en) 2010-04-01 2012-08-21 Bioamber S.A.S. Processes for producing succinic acid from fermentation broths containing diammonium succinate
WO2012143736A1 (fr) * 2011-04-20 2012-10-26 The University Of Manchester Sélection de souches microbiennes améliorées pour la production d'acide succinique à partir de glycérol
WO2013088239A3 (fr) * 2011-12-13 2014-01-03 Bioamber S.A.S. Procédés de purification de l'acide succinique par distillation
US8624059B2 (en) 2010-03-26 2014-01-07 Bioamber S.A.S. Processes for producing monoammonium succinate from fermentation broths containing diammonium succinate, monoammonium succinate and/or succinic acid, and conversion of monoammonium succinate to succinic acid
US20150005510A1 (en) * 2011-09-01 2015-01-01 Myriant Corporation Method for conversion of diammonium succinate in fermentation broth to 2-pyrrolidone and n-methylpyrrolidone
EP2918574A1 (fr) 2009-12-31 2015-09-16 Groupe Novasep SAS Purification d'acide succinique à partir d'un bouillon de fermentation contenant de succinate d'ammonium
WO2016083749A1 (fr) 2014-11-26 2016-06-02 Roquette Freres Procede de recuperation de cristaux d'acide succinique avec mise en œuvre de tensioactifs au cours de la cristallisation et cristaux obtenus
WO2016202880A1 (fr) * 2015-06-15 2016-12-22 Dsm Ip Assets B.V. Procédé de fermentation
WO2017036934A1 (fr) * 2015-09-02 2017-03-09 Thyssenkrupp Industrial Solutions Ag Procédé et installation de récupération de l'acide carboxylique généré au cours d'un processus de fermentation
JP2020114850A (ja) * 2015-07-14 2020-07-30 ピューラック バイオケム ビー．ブイ． コハク酸を製造する方法
KR20230080039A (ko) * 2021-11-29 2023-06-07 주식회사 엘지화학 3-하이드록시프로피온산염의 결정 및 이의 제조 방법
KR102931738B1 (ko) 2021-11-29 2026-02-25 주식회사 엘지화학 알카리 금속의 수산화물 및 3-하이드록시프로피온산의 회수 공정
KR102931737B1 (ko) 2021-11-29 2026-02-25 주식회사 엘지화학 알카리 금속의 수산화물 및 3-하이드록시프로피온산의 회수 공정
KR102931736B1 (ko) 2021-11-29 2026-02-25 주식회사 엘지화학 알카리 금속의 탄산염 및 3-하이드록시프로피온산의 회수 공정

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101051582B1 (ko) * 2009-05-13 2011-07-22 삼성석유화학(주) 발효액으로부터 숙신산을 분리 및 정제하는 방법
TW201538477A (zh) * 2013-12-06 2015-10-16 Myriant Corp 製備丁二酸酯之方法
KR102831417B1 (ko) * 2023-02-07 2025-07-09 씨제이제일제당 주식회사 입도가 조절된 l-시스테인 염산염 수화물 결정의 제조 방법

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265190B1 (en) * 1997-08-18 2001-07-24 Michigan State University Succinic acid production and purification
WO2005030973A1 (fr) * 2003-09-30 2005-04-07 Ajinomoto Co., Inc. Procede de purification d'acide succinique a partir d'un liquide de fermentation
JP2005333886A (ja) * 2004-05-27 2005-12-08 Showa Denko Kk 微生物によるコハク酸の製造方法
KR20060083729A (ko) * 2005-01-18 2006-07-21 한국과학기술원 숙신산 정제 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265190B1 (en) * 1997-08-18 2001-07-24 Michigan State University Succinic acid production and purification
WO2005030973A1 (fr) * 2003-09-30 2005-04-07 Ajinomoto Co., Inc. Procede de purification d'acide succinique a partir d'un liquide de fermentation
JP2005333886A (ja) * 2004-05-27 2005-12-08 Showa Denko Kk 微生物によるコハク酸の製造方法
KR20060083729A (ko) * 2005-01-18 2006-07-21 한국과학기술원 숙신산 정제 방법

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150057425A1 (en) * 2009-11-24 2015-02-26 Dsm Ip Assets B.V. Process for the crystallization of succinic acid
EP2504307A1 (fr) 2009-11-24 2012-10-03 DSM IP Assets B.V. Procédé de cristallisation d'acide succinique
WO2011064151A1 (fr) 2009-11-24 2011-06-03 Dsm Ip Assets B.V. Procédé de cristallisation d'acide succinique
US9233906B2 (en) 2009-12-31 2016-01-12 Group Novasep SAS Purification of succinic acid from the fermentation broth containing ammonium succinate
EP2918574A1 (fr) 2009-12-31 2015-09-16 Groupe Novasep SAS Purification d'acide succinique à partir d'un bouillon de fermentation contenant de succinate d'ammonium
US8624059B2 (en) 2010-03-26 2014-01-07 Bioamber S.A.S. Processes for producing monoammonium succinate from fermentation broths containing diammonium succinate, monoammonium succinate and/or succinic acid, and conversion of monoammonium succinate to succinic acid
EP2371802A1 (fr) 2010-03-30 2011-10-05 DSM IP Assets B.V. Procédé de cristallisation d'acide succinique
US8084626B1 (en) 2010-04-01 2011-12-27 Bioamber S.A.S. Processes for the production of hydrogenated products
US20120021473A1 (en) * 2010-04-01 2012-01-26 Bioamber S.A.S. Processes for producing carboxylic acids from fermentation broths containing their ammonium salts
US8246792B2 (en) 2010-04-01 2012-08-21 Bioamber S.A.S. Processes for producing succinic acid from fermentation broths containing diammonium succinate
US8466300B2 (en) 2010-04-01 2013-06-18 Bioamber International S.A.R.L. Processes for the production of hydrogenated products
WO2012143736A1 (fr) * 2011-04-20 2012-10-26 The University Of Manchester Sélection de souches microbiennes améliorées pour la production d'acide succinique à partir de glycérol
US9422587B2 (en) 2011-04-20 2016-08-23 The University Of Manchester Selection of improved microbial strains for production of succinic acid from glycerol
US20150005510A1 (en) * 2011-09-01 2015-01-01 Myriant Corporation Method for conversion of diammonium succinate in fermentation broth to 2-pyrrolidone and n-methylpyrrolidone
WO2013088239A3 (fr) * 2011-12-13 2014-01-03 Bioamber S.A.S. Procédés de purification de l'acide succinique par distillation
WO2016083749A1 (fr) 2014-11-26 2016-06-02 Roquette Freres Procede de recuperation de cristaux d'acide succinique avec mise en œuvre de tensioactifs au cours de la cristallisation et cristaux obtenus
WO2016202880A1 (fr) * 2015-06-15 2016-12-22 Dsm Ip Assets B.V. Procédé de fermentation
JP2020114850A (ja) * 2015-07-14 2020-07-30 ピューラック バイオケム ビー．ブイ． コハク酸を製造する方法
JP2022028822A (ja) * 2015-07-14 2022-02-16 ピューラック バイオケム ビー．ブイ． コハク酸を製造する方法
WO2017036934A1 (fr) * 2015-09-02 2017-03-09 Thyssenkrupp Industrial Solutions Ag Procédé et installation de récupération de l'acide carboxylique généré au cours d'un processus de fermentation
KR20230080039A (ko) * 2021-11-29 2023-06-07 주식회사 엘지화학 3-하이드록시프로피온산염의 결정 및 이의 제조 방법
KR102921587B1 (ko) 2021-11-29 2026-01-30 주식회사 엘지화학 3-하이드록시프로피온산염의 결정 및 이의 제조 방법
KR102931738B1 (ko) 2021-11-29 2026-02-25 주식회사 엘지화학 알카리 금속의 수산화물 및 3-하이드록시프로피온산의 회수 공정
KR102931737B1 (ko) 2021-11-29 2026-02-25 주식회사 엘지화학 알카리 금속의 수산화물 및 3-하이드록시프로피온산의 회수 공정
KR102931736B1 (ko) 2021-11-29 2026-02-25 주식회사 엘지화학 알카리 금속의 탄산염 및 3-하이드록시프로피온산의 회수 공정

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