WO2009100138A3 - Variants of bacillus stearothermophilus alpha-amylase and uses thereof - Google Patents

Variants of bacillus stearothermophilus alpha-amylase and uses thereof Download PDF

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Publication number
WO2009100138A3
WO2009100138A3 PCT/US2009/033087 US2009033087W WO2009100138A3 WO 2009100138 A3 WO2009100138 A3 WO 2009100138A3 US 2009033087 W US2009033087 W US 2009033087W WO 2009100138 A3 WO2009100138 A3 WO 2009100138A3
Authority
WO
WIPO (PCT)
Prior art keywords
variants
amylase
bacillus stearothermophilus
starch
stearothermophilus alpha
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/033087
Other languages
French (fr)
Other versions
WO2009100138A2 (en
Inventor
Michael J. Pepsin
Sandra W. Ramer
Andrew Shaw
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Priority to BRPI0907750-2A priority Critical patent/BRPI0907750A2/en
Priority to MX2010008358A priority patent/MX2010008358A/en
Priority to JP2010545275A priority patent/JP2011510665A/en
Priority to EP09708204A priority patent/EP2247722A2/en
Priority to CA2713451A priority patent/CA2713451A1/en
Priority to CN2009801041152A priority patent/CN101939421B/en
Publication of WO2009100138A2 publication Critical patent/WO2009100138A2/en
Publication of WO2009100138A3 publication Critical patent/WO2009100138A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Described are variants of Bacillus stearodhermophilus alpha-amylases with mutations at positions 181 or/and 182 having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.
PCT/US2009/033087 2008-02-04 2009-02-04 Variants of bacillus stearothermophilus alpha-amylase and uses thereof Ceased WO2009100138A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BRPI0907750-2A BRPI0907750A2 (en) 2008-02-04 2009-02-04 Bacillus stearothermophilus alpha-amylase variants and uses thereof
MX2010008358A MX2010008358A (en) 2008-02-04 2009-02-04 Variants of bacillus stearothermophilus alpha-amylase and uses thereof.
JP2010545275A JP2011510665A (en) 2008-02-04 2009-02-04 Variants of Bacillus stearothermophilus alpha amylase and their uses
EP09708204A EP2247722A2 (en) 2008-02-04 2009-02-04 Variants of bacillus stearothermophilus alpha-amylase and uses thereof
CA2713451A CA2713451A1 (en) 2008-02-04 2009-02-04 Variants of bacillus stearothermophilus alpha-amylase and uses thereof
CN2009801041152A CN101939421B (en) 2008-02-04 2009-02-04 Variants of bacillus stearothermophilus alpha-amylase and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6363908P 2008-02-04 2008-02-04
US61/063,639 2008-02-04

Publications (2)

Publication Number Publication Date
WO2009100138A2 WO2009100138A2 (en) 2009-08-13
WO2009100138A3 true WO2009100138A3 (en) 2009-12-10

Family

ID=40796285

Family Applications (1)

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PCT/US2009/033087 Ceased WO2009100138A2 (en) 2008-02-04 2009-02-04 Variants of bacillus stearothermophilus alpha-amylase and uses thereof

Country Status (8)

Country Link
US (1) US8722381B2 (en)
EP (1) EP2247722A2 (en)
JP (1) JP2011510665A (en)
CN (1) CN101939421B (en)
BR (1) BRPI0907750A2 (en)
CA (1) CA2713451A1 (en)
MX (1) MX2010008358A (en)
WO (1) WO2009100138A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103608460A (en) * 2010-12-22 2014-02-26 诺维信北美公司 Processes used to produce fermentation products

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9617527B2 (en) 2010-04-14 2017-04-11 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
CA2861678C (en) * 2011-10-11 2021-02-16 Novozymes North America, Inc. Processes for producing fermentation products
IN2014CN04905A (en) * 2011-12-02 2015-09-18 Novozymes As
WO2013181647A2 (en) 2012-06-01 2013-12-05 Danisco Us Inc. Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms
WO2014205355A2 (en) 2013-06-21 2014-12-24 Danisco Us Inc. Compositions and methods for clostridial transformation
US10035973B2 (en) 2013-06-24 2018-07-31 Novozymes A/S Process of extracting oil from thin stillage
US11939552B2 (en) 2013-06-24 2024-03-26 Novozymes A/S Process of recovering oil
WO2017015329A1 (en) * 2015-07-23 2017-01-26 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3421596A4 (en) * 2016-02-26 2019-10-23 Nanjing Bestzyme Bio-engineering Co. Ltd. Alpha amylase variant and use thereof
CN105802940B (en) * 2016-04-18 2019-04-16 广西大学 A kind of bacillus licheniformis high-temperatureα-amylase mutant and its application
CN108841772B (en) * 2018-07-16 2021-03-30 江南大学 A Bacillus subtilis engineering bacterium with high expression of α-amylase
BR112022007697A2 (en) * 2019-10-24 2022-07-12 Danisco Us Inc VARIANT ALPHA-AMYLASE THAT FORMS MALTOPENTAOSE/MALTOHEXAOSE
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Citations (2)

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Publication number Priority date Publication date Assignee Title
US6187576B1 (en) * 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
WO2009061381A2 (en) * 2007-11-05 2009-05-14 Danisco Us Inc., Genencor Division Alpha-amylase variants with altered properties

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US5830837A (en) 1994-11-22 1998-11-03 Novo Nordisk A/S Amylase variants
DK0772684T3 (en) 1994-06-17 2005-12-12 Genencor Int Amylolytic enzymes derived from B. Licheniformis alpha-amylase with improved characteristics
AR000862A1 (en) * 1995-02-03 1997-08-06 Novozymes As VARIANTS OF A MOTHER-AMYLASE, A METHOD TO PRODUCE THE SAME, A DNA STRUCTURE AND A VECTOR OF EXPRESSION, A CELL TRANSFORMED BY SUCH A DNA STRUCTURE AND VECTOR, A DETERGENT ADDITIVE, DETERGENT COMPOSITION, A COMPOSITION FOR AND A COMPOSITION FOR THE ELIMINATION OF
NZ310420A (en) * 1995-06-07 2000-01-28 Bioteknologisk Inst Recombinant hexose oxidase, a method of producing the same and use of such enzyme
ES2432519T3 (en) 1996-04-30 2013-12-04 Novozymes A/S Alpha-amylase mutants
EP1432798B1 (en) * 2001-08-27 2010-11-17 Syngenta Participations AG Self-processing plants and plant parts
CN111484996A (en) * 2003-03-06 2020-08-04 巴斯夫酶有限责任公司 Amylases, nucleic acids encoding them, and methods of making and using them
WO2005092015A2 (en) * 2004-03-19 2005-10-06 Novozymes North America, Inc Liquefaction process
JP2007532117A (en) * 2004-04-08 2007-11-15 ジェネンコー・インターナショナル・インク α-amylase mutant
CA2643809A1 (en) * 2006-02-27 2007-09-07 Edenspace Systems Corporation Energy crops for improved biofuel feedstocks

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6187576B1 (en) * 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
WO2009061381A2 (en) * 2007-11-05 2009-05-14 Danisco Us Inc., Genencor Division Alpha-amylase variants with altered properties

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 19 October 2000 (2000-10-19), "Bacillus amyloliquefaciens clone number 25 protein SEQ ID NO:7.", XP002535926, retrieved from EBI accession no. GSP:AAB12432 Database accession no. AAB12432 *
DATABASE Geneseq [online] 24 March 2005 (2005-03-24), "Alpha-amylase for starch hydrolysis, SEQ ID No:9.", XP002535928, retrieved from EBI accession no. GSP:ADW21539 Database accession no. ADW21539 *
DATABASE Geneseq [online] 4 September 2000 (2000-09-04), "Bacillus amyloliquefaciens Termamyl-like alpha-amylase.", XP002535925, retrieved from EBI accession no. GSP:AAY99606 Database accession no. AAY99606 *
DATABASE UniProt [online] 1 February 2005 (2005-02-01), "SubName: Full=Alpha-amylase; Flags: Fragment;", XP002535927, retrieved from EBI accession no. UNIPROT:Q5MB94 Database accession no. Q5MB94 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103608460A (en) * 2010-12-22 2014-02-26 诺维信北美公司 Processes used to produce fermentation products
CN103608460B (en) * 2010-12-22 2017-08-29 诺维信北美公司 Process for producing fermentation product

Also Published As

Publication number Publication date
JP2011510665A (en) 2011-04-07
CA2713451A1 (en) 2009-08-13
WO2009100138A2 (en) 2009-08-13
US20090226569A1 (en) 2009-09-10
CN101939421B (en) 2013-08-21
CN101939421A (en) 2011-01-05
MX2010008358A (en) 2010-08-30
EP2247722A2 (en) 2010-11-10
US8722381B2 (en) 2014-05-13
BRPI0907750A2 (en) 2015-07-21

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