WO2009100178A3 - New brassica ogura restorer lines with shortened raphanus fragment (srf) - Google Patents

New brassica ogura restorer lines with shortened raphanus fragment (srf) Download PDF

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Publication number
WO2009100178A3
WO2009100178A3 PCT/US2009/033153 US2009033153W WO2009100178A3 WO 2009100178 A3 WO2009100178 A3 WO 2009100178A3 US 2009033153 W US2009033153 W US 2009033153W WO 2009100178 A3 WO2009100178 A3 WO 2009100178A3
Authority
WO
WIPO (PCT)
Prior art keywords
new
srf
lines
raphanus fragment
restorer lines
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/033153
Other languages
French (fr)
Other versions
WO2009100178A2 (en
Inventor
Yongping Zhang
Jayantilal D. Patel
Lomas Tulsieram
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Priority to EA201070928A priority Critical patent/EA201070928A1/en
Priority to AU2009212420A priority patent/AU2009212420B2/en
Priority to CN200980109801.9A priority patent/CN102083989B/en
Priority to EP09708971A priority patent/EP2247734A2/en
Priority to CA2714400A priority patent/CA2714400C/en
Publication of WO2009100178A2 publication Critical patent/WO2009100178A2/en
Publication of WO2009100178A3 publication Critical patent/WO2009100178A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

New Brassica Ogura fertility restorer lines with a shortened Raphanus fragment are provided. The new lines lack the OPC2 marker and are capable of fully restoring fertility in Ogura cytoplasmic male sterile (cms) plants. The improved lines were developed using a new breeding method. The new breeding method can be used to shorten an exotic insertion comprising a gene of interest in any plant.
PCT/US2009/033153 2008-02-06 2009-02-05 New brassica ogura restorer lines with shortened raphanus fragment (srf) Ceased WO2009100178A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EA201070928A EA201070928A1 (en) 2008-02-06 2009-02-05 NEW RESTORATOR-CONTAINING LINES BRASSICA OGURA WITH RAPHANUS CROPPED FRAGMENT (SRF)
AU2009212420A AU2009212420B2 (en) 2008-02-06 2009-02-05 New brassica ogura restorer lines with shortened Raphanus fragment (SRF)
CN200980109801.9A CN102083989B (en) 2008-02-06 2009-02-05 A new restorer line of Brassica Ogura with a shortened Raphanus fragment (SRF)
EP09708971A EP2247734A2 (en) 2008-02-06 2009-02-05 New brassica ogura restorer lines with shortened raphanus fragment (srf)
CA2714400A CA2714400C (en) 2008-02-06 2009-02-05 Brassica ogura restorer line r1931 with shortened raphanus fragment (srf)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US2660408P 2008-02-06 2008-02-06
US61/026,604 2008-02-06
US5485708P 2008-05-21 2008-05-21
US61/054,857 2008-05-21

Publications (2)

Publication Number Publication Date
WO2009100178A2 WO2009100178A2 (en) 2009-08-13
WO2009100178A3 true WO2009100178A3 (en) 2009-11-12

Family

ID=40546050

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/033153 Ceased WO2009100178A2 (en) 2008-02-06 2009-02-05 New brassica ogura restorer lines with shortened raphanus fragment (srf)

Country Status (7)

Country Link
US (3) US8466347B2 (en)
EP (1) EP2247734A2 (en)
CN (2) CN104041403A (en)
AU (1) AU2009212420B2 (en)
CA (1) CA2714400C (en)
EA (1) EA201070928A1 (en)
WO (1) WO2009100178A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102640700A (en) * 2012-05-04 2012-08-22 湖南省作物研究所 Cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and transformation method and application thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016307234C1 (en) * 2015-08-11 2024-09-26 Limagrain Europe Brassica plant with pod shattering tolerance
CN114350832B (en) * 2017-08-24 2023-09-26 湖南省作物研究所 Exogenous radish fragment specific marker and preparation method and application thereof
EP4262358A1 (en) 2020-12-21 2023-10-25 Basf Agricultural Solutions Seed Us Llc Brassica napus plants comprising an improved fertility restorer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997002737A1 (en) * 1995-07-07 1997-01-30 Zenco (No.4) Limited Cytoplasmic male sterility system producing canola hybrids
WO1998056948A1 (en) * 1997-06-10 1998-12-17 Pioneer Hi-Bred International, Inc. USE OF MOLECULAR MARKERS FOR GENOTYPE DETERMINATION OF THE OGURA Rf GENE IN BRASSICA NAPUS
WO2000054574A2 (en) * 1999-03-18 2000-09-21 The University Of Chicago Chemical and physical treatment that stimulate recombination
EP1493328A1 (en) * 2003-07-04 2005-01-05 Institut National De La Recherche Agronomique Method of producing double low restorer lines of brassica napus having a good agronomic value

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2667078B1 (en) * 1990-09-21 1994-09-16 Agronomique Inst Nat Rech DNA SEQUENCE GIVING MALE CYTOPLASMIC STERILITY, MITOCHONDRIAL, MITOCHONDRIA AND PLANT CONTAINING THE SAME, AND PROCESS FOR THE PREPARATION OF HYBRIDS.
CA2193938A1 (en) * 1996-12-24 1998-06-24 David G. Charne Oilseed brassica containing an improved fertility restorer gene for ogura cytoplasmic male sterility
US7314971B2 (en) * 2001-07-12 2008-01-01 Basf Plant Science Gmbh Nuclear fertility restorer genes and methods of use in plants
CA2452633A1 (en) * 2001-07-12 2003-01-23 Mcgill University Nuclear fertility restorer genes and methods of use in plants
GB0402106D0 (en) * 2004-01-30 2004-03-03 Syngenta Participations Ag Improved fertility restoration for ogura cytoplasmic male sterile brassica and method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997002737A1 (en) * 1995-07-07 1997-01-30 Zenco (No.4) Limited Cytoplasmic male sterility system producing canola hybrids
WO1998056948A1 (en) * 1997-06-10 1998-12-17 Pioneer Hi-Bred International, Inc. USE OF MOLECULAR MARKERS FOR GENOTYPE DETERMINATION OF THE OGURA Rf GENE IN BRASSICA NAPUS
WO2000054574A2 (en) * 1999-03-18 2000-09-21 The University Of Chicago Chemical and physical treatment that stimulate recombination
EP1493328A1 (en) * 2003-07-04 2005-01-05 Institut National De La Recherche Agronomique Method of producing double low restorer lines of brassica napus having a good agronomic value

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 2 February 2006 (2006-02-02), "Strongylocentrotus purpuratus clone R3-3024G19, WORKING DRAFT SEQUENCE, 16 unordered pieces.", XP002543896, retrieved from EBI accession no. EMBL:AC180800 Database accession no. AC180800 *
DELOURME R ET AL: "IDENTIFICATION OF RAPD MARKERS LINKED TO A FERTILITY RESTORER GENE FOR THE OGURA RADISH CYTOPLASMIC MALE STERILITY OF RAPESEED (BRASSICA NAPUS L.)", THEORETICAL AND APPLIED GENETICS, SPRINGER, BERLIN, DE, vol. 88, no. 6/07, 1 January 1994 (1994-01-01), pages 741 - 748, XP002043620, ISSN: 0040-5752 *
HONG DENGFENG ET AL: "AFLP and SCAR markers linked to the suppressor gene (Rf) of a dominant genetic male sterility in rapeseed (Brassica napus L.)", EUPHYTICA, KLUWER ACADEMIC PRESS, AMSTERDAM, NL, vol. 151, no. 3, 1 October 2006 (2006-10-01), pages 401 - 409, XP002463859, ISSN: 0014-2336 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102640700A (en) * 2012-05-04 2012-08-22 湖南省作物研究所 Cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and transformation method and application thereof

Also Published As

Publication number Publication date
US8466347B2 (en) 2013-06-18
AU2009212420B2 (en) 2014-07-03
WO2009100178A2 (en) 2009-08-13
CA2714400C (en) 2019-03-19
EA201070928A1 (en) 2011-02-28
CA2714400A1 (en) 2009-08-13
CN104041403A (en) 2014-09-17
AU2009212420A1 (en) 2009-08-13
EP2247734A2 (en) 2010-11-10
US8766040B2 (en) 2014-07-01
CN102083989B (en) 2014-07-02
CN102083989A (en) 2011-06-01
US20140345005A1 (en) 2014-11-20
US20090205065A1 (en) 2009-08-13
US20130254946A1 (en) 2013-09-26

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