WO2009129086A3 - Endoglycosidases that cleave o-linked glycans - Google Patents

Endoglycosidases that cleave o-linked glycans Download PDF

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Publication number
WO2009129086A3
WO2009129086A3 PCT/US2009/039735 US2009039735W WO2009129086A3 WO 2009129086 A3 WO2009129086 A3 WO 2009129086A3 US 2009039735 W US2009039735 W US 2009039735W WO 2009129086 A3 WO2009129086 A3 WO 2009129086A3
Authority
WO
WIPO (PCT)
Prior art keywords
linked glycans
endoglycosidases
cleave
family
neoglycopeptides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/039735
Other languages
French (fr)
Other versions
WO2009129086A2 (en
Inventor
Dimitris Koutsioulis
Ellen Guthrie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Priority to EP09732089A priority Critical patent/EP2281036B1/en
Priority to US12/988,344 priority patent/US8969034B2/en
Publication of WO2009129086A2 publication Critical patent/WO2009129086A2/en
Publication of WO2009129086A3 publication Critical patent/WO2009129086A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01097—Glycopeptide alpha-N-acetylgalactosaminidase (3.2.1.97)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Methods and compositions have been described that relate to a newly identified polypeptide family wherein each member has 0-glycosidase activity and specified sequence characteristics. This family of enzymes can be used for example for cleaving O-linked glycans and for synthesis of neoglycopeptides or neoglycoproteins.
PCT/US2009/039735 2008-04-18 2009-04-07 Endoglycosidases that cleave o-linked glycans Ceased WO2009129086A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09732089A EP2281036B1 (en) 2008-04-18 2009-04-07 Endoglyosidases that cleave o-linked glycans
US12/988,344 US8969034B2 (en) 2008-04-18 2009-04-07 Endoglycosidases that cleave O-linked glycans

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4612908P 2008-04-18 2008-04-18
US61/046,129 2008-04-18

Publications (2)

Publication Number Publication Date
WO2009129086A2 WO2009129086A2 (en) 2009-10-22
WO2009129086A3 true WO2009129086A3 (en) 2010-01-14

Family

ID=40802335

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/039735 Ceased WO2009129086A2 (en) 2008-04-18 2009-04-07 Endoglycosidases that cleave o-linked glycans

Country Status (3)

Country Link
US (1) US8969034B2 (en)
EP (1) EP2281036B1 (en)
WO (1) WO2009129086A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2814835T3 (en) 2012-02-14 2019-06-11 Univ California Enzymes and methods for cleaving N-glycans from glycoproteins
CN111183220B (en) 2017-05-26 2024-03-22 杰诺维斯公司 Tool for glycan analysis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004106367A2 (en) * 2003-05-30 2004-12-09 Intercell Ag Enterococcus antigens
EP1767645A1 (en) * 2005-09-23 2007-03-28 Ajinomoto Co., Inc. Transglycosylation method and method for producing glycoprotein

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5718895A (en) * 1995-11-16 1998-02-17 Alcon Laboratories, Inc. Enzymes with low isoelectric points for use in contact lens cleaning
CN100510067C (en) 1999-07-09 2009-07-08 诺维信公司 Glucoamylase variants
WO2002057460A2 (en) 2000-12-20 2002-07-25 Bristol-Myers Squibb Company Polynucleotides encoding human phosphatases
WO2003091420A2 (en) 2002-04-26 2003-11-06 Codexis, Inc. Ribulose 1,5-bisphosphate carboxylase/oxygenase polypeptides and related polynucleotides
US6740509B2 (en) * 2002-05-22 2004-05-25 Ikuko Ishii Karakasa Method for the production of mucin-type glycopeptide
JP4629439B2 (en) 2002-09-24 2011-02-09 ノボザイムス，インコーポレイティド Microbial trypsin mutant having chymotrypsin activity and nucleic acid encoding the same
US7148051B2 (en) 2004-08-16 2006-12-12 E. I. Du Pont De Nemours And Company Production of 3-hydroxycarboxylic acid using nitrilase
JP4784224B2 (en) 2004-09-24 2011-10-05 味の素株式会社 Glycosyl transfer method and glycosyltransferase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004106367A2 (en) * 2003-05-30 2004-12-09 Intercell Ag Enterococcus antigens
EP1767645A1 (en) * 2005-09-23 2007-03-28 Ajinomoto Co., Inc. Transglycosylation method and method for producing glycoprotein

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE EPO Proteins [online] 2 April 2007 (2007-04-02), "Sequence 12 from Patent EP1767645.", retrieved from EBI accession no. EPOP:CS499397 Database accession no. CS499397 *
DATABASE Geneseq [online] 24 February 2005 (2005-02-24), "E. faecalis V583 hyperimmune serum reactive antigen protein - SEQ ID 446.", XP002535491, retrieved from EBI accession no. GSP:ADV16751 Database accession no. ADV16751 *
DATABASE UniProt [online] 25 November 2008 (2008-11-25), "SubName: Full=Endo-alpha-N-acetylgalactosaminidase; EC=<A HREF="http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?[enzyme-ECNumber:3.2.1.97]+-e">3.2.1.97</A>; Flags: Fragment;", retrieved from EBI accession no. UNIPROT:B5UB72 Database accession no. B5UB72 *
FUJITA K ET AL: "Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-Glycan-specific enda-alpha-N-acetylgalactosam inidase from Bifidobacterium longum", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM, US, vol. 280, 1 September 2005 (2005-09-01), pages 37415 - 37422, XP002383896, ISSN: 0021-9258 *
GODA HM ET AL: "Molecular cloning, expression, and characterisation of a novel endo-alpha-N-acetylgalactosaminidase from Enterococcus faecalis", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 375, 24 August 2008 (2008-08-24), ELSEVIER, NL, AMSTERDAM, pages 441 - 446, XP002535490 *
KATAYAMA T ET AL: "Novel bifidobacterial glycosidases acting on sugar chains of mucin glycoproteins", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 99, no. 5, 1 January 2005 (2005-01-01), pages 457 - 465, XP004997036, ISSN: 1389-1723 *
KOUTSIOULIS DIMITRIS ET AL: "Novel endo-alpha-N-acetylgalactosaminidases with broader substrate specificity", GLYCOBIOLOGY, vol. 18, no. 10, October 2008 (2008-10-01), pages 799 - 805, XP002535489, ISSN: 0959-6658 *

Also Published As

Publication number Publication date
WO2009129086A2 (en) 2009-10-22
US20110053215A1 (en) 2011-03-03
EP2281036A2 (en) 2011-02-09
US8969034B2 (en) 2015-03-03
EP2281036B1 (en) 2013-02-27

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